(Circular) (Six-base) MAPSORT of: pF1KE0467.seq  Check: 1817  from: 1  to: 3764



pF1KE0467  3764 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AccI GT'mk_AC

Cuts at:    802     802 
   Size:       3764
 
Acc65I G'GTAC_C

Cuts at:    780     780 
   Size:       3764
 
AclI AA'CG_TT

Cuts at:   3604    3604 
   Size:       3764
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    595     724    1589    2021    2768    3036     595 
   Size:        129     865     432     747     268    1323

  Fragments arranged by size: 

                1323     865     747     432     268     129
 
AflIII A'CryG_T

Cuts at:   1116    2220    3197    1116 
   Size:       1104     977    1683

  Fragments arranged by size: 

                1683    1104     977
 
AgeI A'CCGG_T

Cuts at:   2152    2152 
   Size:       3764
 
AlwNI CAG_nnn'CTG

Cuts at:   1069    2636    1069 
   Size:       1567    2197
 
ApaI G_GGCC'C

Cuts at:    165     165 
   Size:       3764
 
ApaLI G'TGCA_C

Cuts at:   2534    2534 
   Size:       3764
 
ApoI r'AATT_y

Cuts at:    625     768    3117    3373     625 
   Size:        143    2349     256    1016

  Fragments arranged by size: 

                2349    1016     256     143
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3764
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3764
 
AvaI C'yCGr_G

Cuts at:    156     493     784     156 
   Size:        337     291    3136

  Fragments arranged by size: 

                3136     337     291
 
BamHI G'GATC_C

Cuts at:    789     789 
   Size:       3764
 
BanI G'GyrC_C

Cuts at:    505     780    1194    1442    1477     505 
   Size:        275     414     248      35    2792

  Fragments arranged by size: 

                2792     414     275     248      35
 
BanII G_rGCy'C

Cuts at:    165     778    1808     165 
   Size:        613    1030    2121

  Fragments arranged by size: 

                2121    1030     613
 
BbeI G_GCGC'C

Cuts at:   1198    1446    1198 
   Size:        248    3516
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    655     689     655 
   Size:         34    3730
 
BciVI GTATCCnnnnn_n'

Cuts at:   1662    2423    3764    1662 
   Size:        761    1341    1662

  Fragments arranged by size: 

                1662    1341     761
 
BclI T'GATC_A

Cuts at:   1284    1284 
   Size:       3764
 
BglI GCCn_nnn'nGGC

Cuts at:    124     124 
   Size:       3764
 
BglII A'GATC_T

Cuts at:   1279    3316    1279 
   Size:       2037    1727
 
BlpI GC'TnA_GC

Cuts at:    876     876 
   Size:       3764
 
Bme1580I G_kGCm'C

Cuts at:    165    1389    1482    2538     165 
   Size:       1224      93    1056    1391

  Fragments arranged by size: 

                1391    1224    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    481     976    1057    1392    3536     481 
   Size:        495      81     335    2144     709

  Fragments arranged by size: 

                2144     709     495     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2131    2131 
   Size:       3764
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    671     671 
   Size:       3764
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    664     940    1819    2311    2609     664 
   Size:        276     879     492     298    1819

  Fragments arranged by size: 

                1819     879     492     298     276
 
BsaI GGTCTCn'nnnn_

Cuts at:   3468    3468 
   Size:       3764
 
BsaAI yAC'GTr

Cuts at:   1117    1117 
   Size:       3764
 
BsaBI GATnn'nnATC

Cuts at:      6    1278    3315       6 
   Size:       1272    2037     455

  Fragments arranged by size: 

                2037    1272     455
 
BsaHI Gr'CG_yC

Cuts at:   1195    1443    2145    3641    1195 
   Size:        248     702    1496    1318

  Fragments arranged by size: 

                1496    1318     702     248
 
BsaWI w'CCGG_w

Cuts at:    953    1474    2152    2426    2573    2984     953 
   Size:        521     678     274     147     411    1733

  Fragments arranged by size: 

                1733     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    595     595 
   Size:       3764
 
BseYI C'CCAG_C

Cuts at:    459    1189    1947    2524    3020     459 
   Size:        730     758     577     496    1203

  Fragments arranged by size: 

                1203     758     730     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    501     501 
   Size:       3764
 
BsiEI CG_ry'CG

Cuts at:    104    1306    1352    2560     104 
   Size:       1202      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1202      46
 
BsiHKAI G_wGCw'C

Cuts at:    650     778    1556    1746    2538     650 
   Size:        128     778     190     792    1876

  Fragments arranged by size: 

                1876     792     778     190     128
 
Bsp1286I G_dGCh'C

Cuts at:    165     650     778    1389    1482    1556    1746    1808    2538 
   Size:        485     128     611      93      74     190      62     730

Cuts at:   2538     165 
   Size:       1391

  Fragments arranged by size: 

                1391     730     611     485     190     128      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   2984    2984 
   Size:       3764
 
BspHI T'CATG_A

Cuts at:   2940    2940 
   Size:       3764
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    814    1028    1330    1711     814 
   Size:        214     302     381    2867

  Fragments arranged by size: 

                2867     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2056    2110    3594    3759      50 
   Size:       2006      54    1484     165      55

  Fragments arranged by size: 

                2006    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1676    1676 
   Size:       3764
 
BsrFI r'CCGG_y

Cuts at:   1762    1943    2152    1762 
   Size:        181     209    3374

  Fragments arranged by size: 

                3374     209     181
 
BssSI C'ACGA_G

Cuts at:   2035    2393    2035 
   Size:        358    3406
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1141    1141 
   Size:       3764
 
BstBI TT'CG_AA

Cuts at:   2125    2125 
   Size:       3764
 
BstYI r'GATC_y

Cuts at:      1     789    1256    1279    1614    1860    2872    2958    2970 
   Size:        788     467      23     335     246    1012      86      12

Cuts at:   2970    3316       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     788     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    509     521     509 
   Size:         12    3752
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1714    1895    2058    1714 
   Size:        181     163    3420

  Fragments arranged by size: 

                3420     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1007    1007 
   Size:       3764
 
DraI TTT'AAA

Cuts at:    763     763 
   Size:       3764
 
DrdI GACnn_nn'nnGTC

Cuts at:   1470    2328    1470 
   Size:        858    2906
 
EaeI y'GGCC_r

Cuts at:    427    1349    1523    1914    1941    3286     427 
   Size:        922     174     391      27    1345     905

  Fragments arranged by size: 

                1345     922     905     391     174      27
 
EagI C'GGCC_G

Cuts at:   1349    1349 
   Size:       3764
 
EarI CTCTTCn'nnn_

Cuts at:    527     632     700    1787    1997     527 
   Size:        105      68    1087     210    2294

  Fragments arranged by size: 

                2294    1087     210     105      68
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1972    2282    2428    3574    1972 
   Size:        310     146    1146    2162

  Fragments arranged by size: 

                2162    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    776     776 
   Size:       3764
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    595     671     724    1589    2021    2768    3036     595 
   Size:         76      53     865     432     747     268    1323

  Fragments arranged by size: 

                1323     865     747     432     268      76      53
 
EcoO109I rG'GnC_Cy

Cuts at:    161     585     903     161 
   Size:        424     318    3022

  Fragments arranged by size: 

                3022     424     318
 
EcoRI G'AATT_C

Cuts at:    768     768 
   Size:       3764
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1783    1783 
   Size:       3764
 
FspI TGC'GCA

Cuts at:   1545    1545 
   Size:       3764
 
HaeII r_GCGC'y

Cuts at:   1198    1446    2468    3447    1198 
   Size:        248    1022     979    1515

  Fragments arranged by size: 

                1515    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1606    1636    1638    1668    1606 
   Size:         30       2      30    3702

  Fragments arranged by size: 

                3702      30      30       2
 
HincII GTy'rAC

Cuts at:    803     803 
   Size:       3764
 
KasI G'GCGC_C

Cuts at:   1194    1442    1194 
   Size:        248    3516
 
KpnI G_GTAC'C

Cuts at:    784     784 
   Size:       3764
 
MluI A'CGCG_T

Cuts at:   3197    3197 
   Size:       3764
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    313    2435    2619     313 
   Size:       2122     184    1458

  Fragments arranged by size: 

                2122    1458     184
 
MscI TGG'CCA

Cuts at:   1525    1525 
   Size:       3764
 
MslI CAynn'nnrTG

Cuts at:   1753    1880    1753 
   Size:        127    3637
 
MspA1I CmG'CkG

Cuts at:    250     615     875    1189    1549    2562    2807     250 
   Size:        365     260     314     360    1013     245    1207

  Fragments arranged by size: 

                1207    1013     365     360     314     260     245
 
NaeI GCC'GGC

Cuts at:   1945    1945 
   Size:       3764
 
NarI GG'CG_CC

Cuts at:   1195    1443    1195 
   Size:        248    3516
 
NcoI C'CATG_G

Cuts at:    509     509 
   Size:       3764
 
NgoMIV G'CCGG_C

Cuts at:   1943    1943 
   Size:       3764
 
NspI r_CATG'y

Cuts at:    817    1317    2224     817 
   Size:        500     907    2357

  Fragments arranged by size: 

                2357     907     500
 
PciI A'CATG_T

Cuts at:   2220    2220 
   Size:       3764
 
PmeI GTTT'AAAC

Cuts at:    763     763 
   Size:       3764
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2903    2935    2903 
   Size:         32    3732
 
PpuMI rG'GwC_Cy

Cuts at:    585     585 
   Size:       3764
 
PshAI GACnn'nnGTC

Cuts at:    340     340 
   Size:       3764
 
PspOMI G'GGCC_C

Cuts at:    161     161 
   Size:       3764
 
PstI C_TGCA'G

Cuts at:    811    1496     811 
   Size:        685    3079
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3764
 
PvuII CAG'CTG

Cuts at:    250     615    1189    1549     250 
   Size:        365     574     360    2465

  Fragments arranged by size: 

                2465     574     365     360
 
RsrII CG'GwC_CG

Cuts at:   1959    1959 
   Size:       3764
 
SacI G_AGCT'C

Cuts at:    778     778 
   Size:       3764
 
SalI G'TCGA_C

Cuts at:    801     801 
   Size:       3764
 
SapI GCTCTTCn'nnn_

Cuts at:    632     700    1787    1997     632 
   Size:         68    1087     210    2399

  Fragments arranged by size: 

                2399    1087     210      68
 
SbfI CC_TGCA'GG

Cuts at:    811     811 
   Size:       3764
 
ScaI AGT'ACT

Cuts at:   3281    3281 
   Size:       3764
 
SfcI C'TryA_G

Cuts at:     33     807    1492    2485    2676      33 
   Size:        774     685     993     191    1121

  Fragments arranged by size: 

                1121     993     774     685     191
 
SfoI GGC'GCC

Cuts at:   1196    1444    1196 
   Size:        248    3516
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3764
 
SmaI CCC'GGG

Cuts at:    158     786     158 
   Size:        628    3136
 
SmlI C'TyrA_G

Cuts at:    643     919    1834    2326    2588     643 
   Size:        276     915     492     262    1819

  Fragments arranged by size: 

                1819     915     492     276     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3764
 
SphI G_CATG'C

Cuts at:    817    1317     817 
   Size:        500    3264
 
StuI AGG'CCT

Cuts at:    662     662 
   Size:       3764
 
StyI C'CwwG_G

Cuts at:    509     898    1252     509 
   Size:        389     354    3021

  Fragments arranged by size: 

                3021     389     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1347    1480    1939    2148    1347 
   Size:        133     459     209    2963

  Fragments arranged by size: 

                2963     459     209     133
 
TatI w'GTAC_w

Cuts at:   3279    3279 
   Size:       3764
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    489     521     716     748    3281    3313     489 
   Size:         32     195      32    2533      32     940

  Fragments arranged by size: 

                2533     940     195      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1561    1561 
   Size:       3764
 
XbaI T'CTAG_A

Cuts at:    795     795 
   Size:       3764
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1259    1259 
   Size:       3764
 
XmaI C'CCGG_G

Cuts at:    156     784     156 
   Size:        628    3136
 
XmnI GAAnn'nnTTC

Cuts at:    394     608     394 
   Size:        214    3550
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PmeI     PpiI    PpuMI    PshAI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI    BmgBI     BmtI
   Bpu10I    BsaXI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI     PsiI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XhoI     ZraI