Restriction map for
pF1KE0461
(Circular) (Six-base) MAPSORT of: pF1KE0461.seq Check: 9254 from: 1 to: 3758
pF1KE0461 3758 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 796 796
Size: 3758
Acc65I G'GTAC_C
Cuts at: 774 774
Size: 3758
AclI AA'CG_TT
Cuts at: 3598 3598
Size: 3758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 674 1583 2015 2762 3030 557
Size: 117 909 432 747 268 1285
Fragments arranged by size:
1285 909 747 432 268 117
AflII C'TTAA_G
Cuts at: 411 411
Size: 3758
AflIII A'CryG_T
Cuts at: 1110 2214 3191 1110
Size: 1104 977 1677
Fragments arranged by size:
1677 1104 977
AgeI A'CCGG_T
Cuts at: 2146 2146
Size: 3758
AlwNI CAG_nnn'CTG
Cuts at: 1063 2630 1063
Size: 1567 2191
ApaLI G'TGCA_C
Cuts at: 2528 2528
Size: 3758
ApoI r'AATT_y
Cuts at: 211 247 762 3111 3367 211
Size: 36 515 2349 256 602
Fragments arranged by size:
2349 602 515 256 36
AseI AT'TA_AT
Cuts at: 20 20
Size: 3758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
AvaI C'yCGr_G
Cuts at: 778 778
Size: 3758
BamHI G'GATC_C
Cuts at: 783 783
Size: 3758
BanI G'GyrC_C
Cuts at: 774 1188 1436 1471 774
Size: 414 248 35 3061
Fragments arranged by size:
3061 414 248 35
BanII G_rGCy'C
Cuts at: 369 772 1802 369
Size: 403 1030 2325
Fragments arranged by size:
2325 1030 403
BbeI G_GCGC'C
Cuts at: 1192 1440 1192
Size: 248 3510
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 550 584 550
Size: 34 3724
BciVI GTATCCnnnnn_n'
Cuts at: 297 1656 2417 3758 297
Size: 1359 761 1341 297
Fragments arranged by size:
1359 1341 761 297
BclI T'GATC_A
Cuts at: 1278 1278
Size: 3758
BglII A'GATC_T
Cuts at: 1273 3310 1273
Size: 2037 1721
BlpI GC'TnA_GC
Cuts at: 870 870
Size: 3758
Bme1580I G_kGCm'C
Cuts at: 1383 1476 2532 1383
Size: 93 1056 2609
Fragments arranged by size:
2609 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 970 1051 1386 3530 970
Size: 81 335 2144 1198
Fragments arranged by size:
2144 1198 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 3758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 934 1813 2305 2603 625
Size: 309 879 492 298 1780
Fragments arranged by size:
1780 879 492 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 3462 3462
Size: 3758
BsaAI yAC'GTr
Cuts at: 1111 1111
Size: 3758
BsaBI GATnn'nnATC
Cuts at: 6 1272 3309 6
Size: 1266 2037 455
Fragments arranged by size:
2037 1266 455
BsaHI Gr'CG_yC
Cuts at: 1189 1437 2139 3635 1189
Size: 248 702 1496 1312
Fragments arranged by size:
1496 1312 702 248
BsaWI w'CCGG_w
Cuts at: 947 1468 2146 2420 2567 2978 947
Size: 521 678 274 147 411 1727
Fragments arranged by size:
1727 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 572 602 572
Size: 30 3728
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 368 368
Size: 3758
BseYI C'CCAG_C
Cuts at: 1183 1941 2518 3014 1183
Size: 758 577 496 1927
Fragments arranged by size:
1927 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 101
Size: 3758
BsiEI CG_ry'CG
Cuts at: 104 1300 1346 2554 104
Size: 1196 46 1208 1308
Fragments arranged by size:
1308 1208 1196 46
BsiHKAI G_wGCw'C
Cuts at: 369 772 1550 1740 2532 369
Size: 403 778 190 792 1595
Fragments arranged by size:
1595 792 778 403 190
BsmI GAATG_Cn'
Cuts at: 663 663
Size: 3758
Bsp1286I G_dGCh'C
Cuts at: 369 772 1383 1476 1550 1740 1802 2532 369
Size: 403 611 93 74 190 62 730 1595
Fragments arranged by size:
1595 730 611 403 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2978 2978
Size: 3758
BspHI T'CATG_A
Cuts at: 2934 2934
Size: 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 808 1022 1324 1705 808
Size: 214 302 381 2861
Fragments arranged by size:
2861 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2050 2104 3588 3753 50
Size: 2000 54 1484 165 55
Fragments arranged by size:
2000 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1670 1670
Size: 3758
BsrFI r'CCGG_y
Cuts at: 707 1756 1937 2146 707
Size: 1049 181 209 2319
Fragments arranged by size:
2319 1049 209 181
BsrGI T'GTAC_A
Cuts at: 675 675
Size: 3758
BssSI C'ACGA_G
Cuts at: 2029 2387 2029
Size: 358 3400
BstAPI GCAn_nnn'nTGC
Cuts at: 1135 1135
Size: 3758
BstBI TT'CG_AA
Cuts at: 2119 2119
Size: 3758
BstYI r'GATC_y
Cuts at: 1 651 783 1250 1273 1608 1854 2866 2952
Size: 650 132 467 23 335 246 1012 86
Cuts at: 2952 2964 3310 1
Size: 12 346 449
Fragments arranged by size:
1012 650 467 449 346 335 246 132
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3758
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1708 1889 2052 1708
Size: 181 163 3414
Fragments arranged by size:
3414 181 163
BtsI GCAGTG_nn'
Cuts at: 1001 1001
Size: 3758
DraI TTT'AAA
Cuts at: 144 757 144
Size: 613 3145
DrdI GACnn_nn'nnGTC
Cuts at: 1464 2322 1464
Size: 858 2900
EaeI y'GGCC_r
Cuts at: 276 1343 1517 1908 1935 3280 276
Size: 1067 174 391 27 1345 754
Fragments arranged by size:
1345 1067 754 391 174 27
EagI C'GGCC_G
Cuts at: 1343 1343
Size: 3758
EarI CTCTTCn'nnn_
Cuts at: 1781 1991 1781
Size: 210 3548
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1966 2276 2422 3568 1966
Size: 310 146 1146 2156
Fragments arranged by size:
2156 1146 310 146
EcoICRI GAG'CTC
Cuts at: 367 770 367
Size: 403 3355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 674 1583 2015 2762 3030 557
Size: 117 909 432 747 268 1285
Fragments arranged by size:
1285 909 747 432 268 117
EcoO109I rG'GnC_Cy
Cuts at: 897 897
Size: 3758
EcoRI G'AATT_C
Cuts at: 762 762
Size: 3758
EcoRV GAT'ATC
Cuts at: 384 384
Size: 3758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1777 1777
Size: 3758
FspI TGC'GCA
Cuts at: 1539 1539
Size: 3758
HaeII r_GCGC'y
Cuts at: 1192 1440 2462 3441 1192
Size: 248 1022 979 1509
Fragments arranged by size:
1509 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1600 1630 1632 1662 1600
Size: 30 2 30 3696
Fragments arranged by size:
3696 30 30 2
HincII GTy'rAC
Cuts at: 425 797 425
Size: 372 3386
HindIII A'AGCT_T
Cuts at: 645 645
Size: 3758
KasI G'GCGC_C
Cuts at: 1188 1436 1188
Size: 248 3510
KpnI G_GTAC'C
Cuts at: 778 778
Size: 3758
MfeI C'AATT_G
Cuts at: 236 236
Size: 3758
MluI A'CGCG_T
Cuts at: 3191 3191
Size: 3758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2429 2613 2429
Size: 184 3574
MscI TGG'CCA
Cuts at: 278 1519 278
Size: 1241 2517
MslI CAynn'nnrTG
Cuts at: 1747 1874 1747
Size: 127 3631
MspA1I CmG'CkG
Cuts at: 419 869 1183 1543 2556 2801 419
Size: 450 314 360 1013 245 1376
Fragments arranged by size:
1376 1013 450 360 314 245
NaeI GCC'GGC
Cuts at: 709 1939 709
Size: 1230 2528
NarI GG'CG_CC
Cuts at: 1189 1437 1189
Size: 248 3510
NcoI C'CATG_G
Cuts at: 107 107
Size: 3758
NdeI CA'TA_TG
Cuts at: 513 513
Size: 3758
NgoMIV G'CCGG_C
Cuts at: 707 1937 707
Size: 1230 2528
NspI r_CATG'y
Cuts at: 811 1311 2218 811
Size: 500 907 2351
Fragments arranged by size:
2351 907 500
PciI A'CATG_T
Cuts at: 2214 2214
Size: 3758
PmeI GTTT'AAAC
Cuts at: 757 757
Size: 3758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 3726
PstI C_TGCA'G
Cuts at: 296 805 1490 296
Size: 509 685 2564
Fragments arranged by size:
2564 685 509
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3758
PvuII CAG'CTG
Cuts at: 419 1183 1543 419
Size: 764 360 2634
Fragments arranged by size:
2634 764 360
RsrII CG'GwC_CG
Cuts at: 1953 1953
Size: 3758
SacI G_AGCT'C
Cuts at: 369 772 369
Size: 403 3355
SalI G'TCGA_C
Cuts at: 795 795
Size: 3758
SapI GCTCTTCn'nnn_
Cuts at: 1781 1991 1781
Size: 210 3548
SbfI CC_TGCA'GG
Cuts at: 805 805
Size: 3758
ScaI AGT'ACT
Cuts at: 3275 3275
Size: 3758
SfcI C'TryA_G
Cuts at: 33 292 348 801 1486 2479 2670 33
Size: 259 56 453 685 993 191 1121
Fragments arranged by size:
1121 993 685 453 259 191 56
SfoI GGC'GCC
Cuts at: 1190 1438 1190
Size: 248 3510
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
SmaI CCC'GGG
Cuts at: 780 780
Size: 3758
SmlI C'TyrA_G
Cuts at: 411 604 913 1828 2320 2582 411
Size: 193 309 915 492 262 1587
Fragments arranged by size:
1587 915 492 309 262 193
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3758
SphI G_CATG'C
Cuts at: 811 1311 811
Size: 500 3258
StyI C'CwwG_G
Cuts at: 107 892 1246 107
Size: 785 354 2619
Fragments arranged by size:
2619 785 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1341 1474 1933 2142 1341
Size: 133 459 209 2957
Fragments arranged by size:
2957 459 209 133
TatI w'GTAC_w
Cuts at: 397 675 3273 397
Size: 278 2598 882
Fragments arranged by size:
2598 882 278
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 3726
Tth111I GACn'n_nGTC
Cuts at: 1555 1555
Size: 3758
XbaI T'CTAG_A
Cuts at: 789 789
Size: 3758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1253 1253
Size: 3758
XmaI C'CCGG_G
Cuts at: 778 778
Size: 3758
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI
HpaI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI