Restriction map for
pF1KE0458
(Circular) (Six-base) MAPSORT of: pF1KE0458.seq Check: 1778 from: 1 to: 3791
pF1KE0458 3791 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 829 829
Size: 3791
Acc65I G'GTAC_C
Cuts at: 807 807
Size: 3791
AclI AA'CG_TT
Cuts at: 3631 3631
Size: 3791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2048 2795 3063 1616
Size: 432 747 268 2344
Fragments arranged by size:
2344 747 432 268
AfeI AGC'GCT
Cuts at: 389 389
Size: 3791
AflIII A'CryG_T
Cuts at: 1143 2247 3224 1143
Size: 1104 977 1710
Fragments arranged by size:
1710 1104 977
AgeI A'CCGG_T
Cuts at: 2179 2179
Size: 3791
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 136 168 232 264 566 598 136
Size: 32 64 32 302 32 3329
Fragments arranged by size:
3329 302 64 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1096 2663 1096
Size: 1567 2224
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 795 3144 3400 795
Size: 2349 256 1186
Fragments arranged by size:
2349 1186 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 256 811 256
Size: 555 3236
BamHI G'GATC_C
Cuts at: 816 816
Size: 3791
BanI G'GyrC_C
Cuts at: 628 807 1221 1469 1504 628
Size: 179 414 248 35 2915
Fragments arranged by size:
2915 414 248 179 35
BanII G_rGCy'C
Cuts at: 173 805 1835 173
Size: 632 1030 2129
Fragments arranged by size:
2129 1030 632
BbeI G_GCGC'C
Cuts at: 1225 1473 1225
Size: 248 3543
BbsI GAAGACnn'nnnn_
Cuts at: 516 516
Size: 3791
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 288 322 337 371 288
Size: 34 15 34 3708
Fragments arranged by size:
3708 34 34 15
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 1311 1311
Size: 3791
BglII A'GATC_T
Cuts at: 1306 3343 1306
Size: 2037 1754
BlpI GC'TnA_GC
Cuts at: 722 903 722
Size: 181 3610
Bme1580I G_kGCm'C
Cuts at: 1416 1509 2565 1416
Size: 93 1056 2642
Fragments arranged by size:
2642 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 361 1003 1084 1419 3563 361
Size: 642 81 335 2144 589
Fragments arranged by size:
2144 642 589 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 3791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 733 494
Size: 239 3552
Bpu10I CC'TnA_GC
Cuts at: 289 289
Size: 3791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1846 2338 2636 967
Size: 879 492 298 2122
Fragments arranged by size:
2122 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3495 3495
Size: 3791
BsaAI yAC'GTr
Cuts at: 1144 1144
Size: 3791
BsaBI GATnn'nnATC
Cuts at: 6 1305 3342 6
Size: 1299 2037 455
Fragments arranged by size:
2037 1299 455
BsaHI Gr'CG_yC
Cuts at: 1222 1470 2172 3668 1222
Size: 248 702 1496 1345
Fragments arranged by size:
1496 1345 702 248
BsaWI w'CCGG_w
Cuts at: 980 1501 2179 2453 2600 3011 980
Size: 521 678 274 147 411 1760
Fragments arranged by size:
1760 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 549 594 698 1216 1974 2551 3047 549
Size: 45 104 518 758 577 496 1293
Fragments arranged by size:
1293 758 577 518 496 104 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 636 636
Size: 3791
BsiEI CG_ry'CG
Cuts at: 104 1333 1379 2587 104
Size: 1229 46 1208 1308
Fragments arranged by size:
1308 1229 1208 46
BsiHKAI G_wGCw'C
Cuts at: 665 805 1583 1773 2565 665
Size: 140 778 190 792 1891
Fragments arranged by size:
1891 792 778 190 140
Bsp1286I G_dGCh'C
Cuts at: 173 665 805 1416 1509 1583 1773 1835 2565
Size: 492 140 611 93 74 190 62 730
Cuts at: 2565 173
Size: 1399
Fragments arranged by size:
1399 730 611 492 190 140 93 74
62
BspEI T'CCGG_A
Cuts at: 3011 3011
Size: 3791
BspHI T'CATG_A
Cuts at: 2967 2967
Size: 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 428 789 841 1055 1357 1738 428
Size: 361 52 214 302 381 2481
Fragments arranged by size:
2481 381 361 302 214 52
BsrBI CCG'CTC
Cuts at: 50 2083 2137 3621 3786 50
Size: 2033 54 1484 165 55
Fragments arranged by size:
2033 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1703 1703
Size: 3791
BsrFI r'CCGG_y
Cuts at: 1789 1970 2179 1789
Size: 181 209 3401
Fragments arranged by size:
3401 209 181
BssHII G'CGCG_C
Cuts at: 268 268
Size: 3791
BssSI C'ACGA_G
Cuts at: 2062 2420 2062
Size: 358 3433
BstAPI GCAn_nnn'nTGC
Cuts at: 693 1168 693
Size: 475 3316
BstBI TT'CG_AA
Cuts at: 2152 2152
Size: 3791
BstXI CCAn_nnnn'nTGG
Cuts at: 639 639
Size: 3791
BstYI r'GATC_y
Cuts at: 1 126 377 440 816 1283 1306 1641 1887
Size: 125 251 63 376 467 23 335 246
Cuts at: 1887 2899 2985 2997 3343 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 376 346 335 251 246
125 86 63 23 12
BtgI C'CryG_G
Cuts at: 107 157 624 107
Size: 50 467 3274
Fragments arranged by size:
3274 467 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1741 1922 2085 1741
Size: 181 163 3447
Fragments arranged by size:
3447 181 163
BtsI GCAGTG_nn'
Cuts at: 1034 1034
Size: 3791
DraI TTT'AAA
Cuts at: 790 790
Size: 3791
DrdI GACnn_nn'nnGTC
Cuts at: 520 1497 2355 520
Size: 977 858 1956
Fragments arranged by size:
1956 977 858
EaeI y'GGCC_r
Cuts at: 1376 1550 1941 1968 3313 1376
Size: 174 391 27 1345 1854
Fragments arranged by size:
1854 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1376 1376
Size: 3791
EarI CTCTTCn'nnn_
Cuts at: 190 1814 2024 190
Size: 1624 210 1957
Fragments arranged by size:
1957 1624 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1999 2309 2455 3601 127
Size: 1872 310 146 1146 317
Fragments arranged by size:
1872 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 803 803
Size: 3791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 733 1616 2048 2795 3063 494
Size: 239 883 432 747 268 1222
Fragments arranged by size:
1222 883 747 432 268 239
EcoO109I rG'GnC_Cy
Cuts at: 248 599 620 930 248
Size: 351 21 310 3109
Fragments arranged by size:
3109 351 310 21
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
EcoRV GAT'ATC
Cuts at: 561 561
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1810 1810
Size: 3791
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 391 1225 1473 2495 3474 391
Size: 834 248 1022 979 708
Fragments arranged by size:
1022 979 834 708 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 130 150 162 172 204 501 533 1633
Size: 12 20 12 10 32 297 32 1100
Cuts at: 1633 1663 1665 1695 118
Size: 30 2 30 2214
Fragments arranged by size:
2214 1100 297 32 32 30 30 20
12 12 10 2
HincII GTy'rAC
Cuts at: 747 830 747
Size: 83 3708
KasI G'GCGC_C
Cuts at: 1221 1469 1221
Size: 248 3543
KpnI G_GTAC'C
Cuts at: 811 811
Size: 3791
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 122 630 732 2462 2646 122
Size: 508 102 1730 184 1267
Fragments arranged by size:
1730 1267 508 184 102
MscI TGG'CCA
Cuts at: 1552 1552
Size: 3791
MslI CAynn'nnrTG
Cuts at: 1780 1907 1780
Size: 127 3664
MspA1I CmG'CkG
Cuts at: 159 472 726 902 1216 1576 2589 2834 159
Size: 313 254 176 314 360 1013 245 1116
Fragments arranged by size:
1116 1013 360 314 313 254 245 176
NaeI GCC'GGC
Cuts at: 1972 1972
Size: 3791
NarI GG'CG_CC
Cuts at: 1222 1470 1222
Size: 248 3543
NcoI C'CATG_G
Cuts at: 107 624 107
Size: 517 3274
NgoMIV G'CCGG_C
Cuts at: 1970 1970
Size: 3791
NspI r_CATG'y
Cuts at: 223 844 1344 2251 223
Size: 621 500 907 1763
Fragments arranged by size:
1763 907 621 500
PciI A'CATG_T
Cuts at: 2247 2247
Size: 3791
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3759
PpuMI rG'GwC_Cy
Cuts at: 248 599 248
Size: 351 3440
PstI C_TGCA'G
Cuts at: 395 838 1523 395
Size: 443 685 2663
Fragments arranged by size:
2663 685 443
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 472 1216 1576 472
Size: 744 360 2687
Fragments arranged by size:
2687 744 360
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 805 805
Size: 3791
SacII CC_GC'GG
Cuts at: 160 160
Size: 3791
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SanDI GG'GwC_CC
Cuts at: 248 599 248
Size: 351 3440
SapI GCTCTTCn'nnn_
Cuts at: 190 1814 2024 190
Size: 1624 210 1957
Fragments arranged by size:
1957 1624 210
SbfI CC_TGCA'GG
Cuts at: 838 838
Size: 3791
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SexAI A'CCwGG_T
Cuts at: 542 542
Size: 3791
SfcI C'TryA_G
Cuts at: 33 391 753 834 1519 2512 2703 33
Size: 358 362 81 685 993 191 1121
Fragments arranged by size:
1121 993 685 362 358 191 81
SfoI GGC'GCC
Cuts at: 1223 1471 1223
Size: 248 3543
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SmaI CCC'GGG
Cuts at: 813 813
Size: 3791
SmlI C'TyrA_G
Cuts at: 946 1861 2353 2615 946
Size: 915 492 262 2122
Fragments arranged by size:
2122 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 844 1344 844
Size: 500 3291
StyI C'CwwG_G
Cuts at: 107 624 925 1279 107
Size: 517 301 354 2619
Fragments arranged by size:
2619 517 354 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 343 1374 1507 1966 2175 343
Size: 1031 133 459 209 1959
Fragments arranged by size:
1959 1031 459 209 133
TatI w'GTAC_w
Cuts at: 3306 3306
Size: 3791
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 3759
Tth111I GACn'n_nGTC
Cuts at: 1588 1588
Size: 3791
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1286 1286
Size: 3791
XmaI C'CCGG_G
Cuts at: 811 811
Size: 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI