Restriction map for
pF1KE0444
(Circular) (Six-base) MAPSORT of: pF1KE0444.seq Check: 420 from: 1 to: 3944
pF1KE0444 3944 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 982 982
Size: 3944
Acc65I G'GTAC_C
Cuts at: 960 960
Size: 3944
AclI AA'CG_TT
Cuts at: 3784 3784
Size: 3944
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1769 2201 2948 3216 1769
Size: 432 747 268 2497
Fragments arranged by size:
2497 747 432 268
AfeI AGC'GCT
Cuts at: 383 383
Size: 3944
AflIII A'CryG_T
Cuts at: 1296 2400 3377 1296
Size: 1104 977 1863
Fragments arranged by size:
1863 1104 977
AgeI A'CCGG_T
Cuts at: 2332 2332
Size: 3944
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 793 825 793
Size: 32 3912
AlwNI CAG_nnn'CTG
Cuts at: 844 1249 2816 844
Size: 405 1567 1972
Fragments arranged by size:
1972 1567 405
ApaLI G'TGCA_C
Cuts at: 2714 2714
Size: 3944
ApoI r'AATT_y
Cuts at: 948 3297 3553 948
Size: 2349 256 1339
Fragments arranged by size:
2349 1339 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3944
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3944
AvaI C'yCGr_G
Cuts at: 377 964 377
Size: 587 3357
BamHI G'GATC_C
Cuts at: 969 969
Size: 3944
BanI G'GyrC_C
Cuts at: 685 960 1374 1622 1657 685
Size: 275 414 248 35 2972
Fragments arranged by size:
2972 414 275 248 35
BanII G_rGCy'C
Cuts at: 958 1988 958
Size: 1030 2914
BbeI G_GCGC'C
Cuts at: 1378 1626 1378
Size: 248 3696
BbsI GAAGACnn'nnnn_
Cuts at: 128 128
Size: 3944
BbvCI CC'TCA_GC
Cuts at: 449 449
Size: 3944
BciVI GTATCCnnnnn_n'
Cuts at: 1842 2603 3944 1842
Size: 761 1341 1842
Fragments arranged by size:
1842 1341 761
BclI T'GATC_A
Cuts at: 207 1464 207
Size: 1257 2687
BfrBI ATG'CAT
Cuts at: 175 175
Size: 3944
BglII A'GATC_T
Cuts at: 1459 3496 1459
Size: 2037 1907
BlpI GC'TnA_GC
Cuts at: 1056 1056
Size: 3944
Bme1580I G_kGCm'C
Cuts at: 1569 1662 2718 1569
Size: 93 1056 2795
Fragments arranged by size:
2795 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1156 1237 1572 3716 1156
Size: 81 335 2144 1384
Fragments arranged by size:
2144 1384 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 555 2311 555
Size: 1756 2188
Bpu10I CC'TnA_GC
Cuts at: 449 449
Size: 3944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1999 2491 2789 1120
Size: 879 492 298 2275
Fragments arranged by size:
2275 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3648 3648
Size: 3944
BsaAI yAC'GTr
Cuts at: 1297 1297
Size: 3944
BsaBI GATnn'nnATC
Cuts at: 6 1458 3495 6
Size: 1452 2037 455
Fragments arranged by size:
2037 1452 455
BsaHI Gr'CG_yC
Cuts at: 1375 1623 2325 3821 1375
Size: 248 702 1496 1498
Fragments arranged by size:
1498 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1133 1654 2332 2606 2753 3164 1133
Size: 521 678 274 147 411 1913
Fragments arranged by size:
1913 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 358 388 358
Size: 30 3914
BseYI C'CCAG_C
Cuts at: 850 1369 2127 2704 3200 850
Size: 519 758 577 496 1594
Fragments arranged by size:
1594 758 577 519 496
BsiEI CG_ry'CG
Cuts at: 104 1486 1532 2740 104
Size: 1382 46 1208 1308
Fragments arranged by size:
1382 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 958 1736 1926 2718 958
Size: 778 190 792 2184
Fragments arranged by size:
2184 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 424 424
Size: 3944
Bsp1286I G_dGCh'C
Cuts at: 958 1569 1662 1736 1926 1988 2718 958
Size: 611 93 74 190 62 730 2184
Fragments arranged by size:
2184 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3164 3164
Size: 3944
BspHI T'CATG_A
Cuts at: 3120 3120
Size: 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 994 1208 1510 1891 994
Size: 214 302 381 3047
Fragments arranged by size:
3047 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2236 2290 3774 3939 50
Size: 2186 54 1484 165 55
Fragments arranged by size:
2186 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1856 1856
Size: 3944
BsrFI r'CCGG_y
Cuts at: 1942 2123 2332 1942
Size: 181 209 3554
Fragments arranged by size:
3554 209 181
BssSI C'ACGA_G
Cuts at: 2215 2573 2215
Size: 358 3586
BstAPI GCAn_nnn'nTGC
Cuts at: 1321 1321
Size: 3944
BstBI TT'CG_AA
Cuts at: 777 2305 777
Size: 1528 2416
BstEII G'GTnAC_C
Cuts at: 534 534
Size: 3944
BstYI r'GATC_y
Cuts at: 1 969 1436 1459 1794 2040 3052 3138 3150
Size: 968 467 23 335 246 1012 86 12
Cuts at: 3150 3496 1
Size: 346 449
Fragments arranged by size:
1012 968 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 649 649
Size: 3944
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 803 1894 2075 2238 803
Size: 1091 181 163 2509
Fragments arranged by size:
2509 1091 181 163
BtsI GCAGTG_nn'
Cuts at: 182 225 1187 182
Size: 43 962 2939
Fragments arranged by size:
2939 962 43
DraI TTT'AAA
Cuts at: 943 943
Size: 3944
DrdI GACnn_nn'nnGTC
Cuts at: 1650 2508 1650
Size: 858 3086
EaeI y'GGCC_r
Cuts at: 1529 1703 2094 2121 3466 1529
Size: 174 391 27 1345 2007
Fragments arranged by size:
2007 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1529 1529
Size: 3944
EarI CTCTTCn'nnn_
Cuts at: 1967 2177 1967
Size: 210 3734
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2152 2462 2608 3754 2152
Size: 310 146 1146 2342
Fragments arranged by size:
2342 1146 310 146
EcoICRI GAG'CTC
Cuts at: 956 956
Size: 3944
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1769 2201 2948 3216 1769
Size: 432 747 268 2497
Fragments arranged by size:
2497 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 917 1083 917
Size: 166 3778
EcoRI G'AATT_C
Cuts at: 948 948
Size: 3944
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 706 1963 706
Size: 1257 2687
FspI TGC'GCA
Cuts at: 1725 1725
Size: 3944
HaeII r_GCGC'y
Cuts at: 385 1378 1626 2648 3627 385
Size: 993 248 1022 979 702
Fragments arranged by size:
1022 993 979 702 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 341 358 373 390 468 500 1786 1816 1818
Size: 17 15 17 78 32 1286 30 2
Cuts at: 1818 1848 341
Size: 30 2437
Fragments arranged by size:
2437 1286 78 32 30 30 17 17
15 2
HincII GTy'rAC
Cuts at: 983 983
Size: 3944
KasI G'GCGC_C
Cuts at: 1374 1622 1374
Size: 248 3696
KpnI G_GTAC'C
Cuts at: 964 964
Size: 3944
MluI A'CGCG_T
Cuts at: 3377 3377
Size: 3944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 344 2615 2799 344
Size: 2271 184 1489
Fragments arranged by size:
2271 1489 184
MscI TGG'CCA
Cuts at: 1705 1705
Size: 3944
MslI CAynn'nnrTG
Cuts at: 218 1933 2060 218
Size: 1715 127 2102
Fragments arranged by size:
2102 1715 127
MspA1I CmG'CkG
Cuts at: 1055 1369 1729 2742 2987 1055
Size: 314 360 1013 245 2012
Fragments arranged by size:
2012 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2125 2125
Size: 3944
NarI GG'CG_CC
Cuts at: 1375 1623 1375
Size: 248 3696
NgoMIV G'CCGG_C
Cuts at: 2123 2123
Size: 3944
NsiI A_TGCA'T
Cuts at: 177 177
Size: 3944
NspI r_CATG'y
Cuts at: 129 997 1497 2404 129
Size: 868 500 907 1669
Fragments arranged by size:
1669 907 868 500
PciI A'CATG_T
Cuts at: 2400 2400
Size: 3944
PmeI GTTT'AAAC
Cuts at: 943 943
Size: 3944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3083 3115 3083
Size: 32 3912
PstI C_TGCA'G
Cuts at: 991 1676 991
Size: 685 3259
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3944
PvuII CAG'CTG
Cuts at: 1369 1729 1369
Size: 360 3584
RsrII CG'GwC_CG
Cuts at: 2139 2139
Size: 3944
SacI G_AGCT'C
Cuts at: 958 958
Size: 3944
SalI G'TCGA_C
Cuts at: 981 981
Size: 3944
SapI GCTCTTCn'nnn_
Cuts at: 1967 2177 1967
Size: 210 3734
SbfI CC_TGCA'GG
Cuts at: 991 991
Size: 3944
ScaI AGT'ACT
Cuts at: 554 3461 554
Size: 2907 1037
SfcI C'TryA_G
Cuts at: 33 987 1672 2665 2856 33
Size: 954 685 993 191 1121
Fragments arranged by size:
1121 993 954 685 191
SfoI GGC'GCC
Cuts at: 1376 1624 1376
Size: 248 3696
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3944
SmaI CCC'GGG
Cuts at: 966 966
Size: 3944
SmlI C'TyrA_G
Cuts at: 1099 2014 2506 2768 1099
Size: 915 492 262 2275
Fragments arranged by size:
2275 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3944
SphI G_CATG'C
Cuts at: 997 1497 997
Size: 500 3444
StuI AGG'CCT
Cuts at: 635 635
Size: 3944
StyI C'CwwG_G
Cuts at: 630 1078 1432 630
Size: 448 354 3142
Fragments arranged by size:
3142 448 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1527 1660 2119 2328 1527
Size: 133 459 209 3143
Fragments arranged by size:
3143 459 209 133
TatI w'GTAC_w
Cuts at: 552 3459 552
Size: 2907 1037
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3461 3493 3461
Size: 32 3912
Tth111I GACn'n_nGTC
Cuts at: 1741 1741
Size: 3944
XbaI T'CTAG_A
Cuts at: 975 975
Size: 3944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 756 1439 756
Size: 683 3261
XmaI C'CCGG_G
Cuts at: 964 964
Size: 3944
XmnI GAAnn'nnTTC
Cuts at: 574 574
Size: 3944
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BpmI BseRI
BsgI BsiWI BsmI BsrGI BssHII BstXI BstZ17I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI