Restriction map for
pF1KE0441
(Circular) (Six-base) MAPSORT of: pF1KE0441.seq Check: 9530 from: 1 to: 3812
pF1KE0441 3812 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 850 850
Size: 3812
Acc65I G'GTAC_C
Cuts at: 828 828
Size: 3812
AclI AA'CG_TT
Cuts at: 3652 3652
Size: 3812
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1637 2069 2816 3084 689
Size: 948 432 747 268 1417
Fragments arranged by size:
1417 948 747 432 268
AflIII A'CryG_T
Cuts at: 1164 2268 3245 1164
Size: 1104 977 1731
Fragments arranged by size:
1731 1104 977
AgeI A'CCGG_T
Cuts at: 2200 2200
Size: 3812
AleI CACnn'nnGTG
Cuts at: 144 144
Size: 3812
AlwNI CAG_nnn'CTG
Cuts at: 1117 2684 1117
Size: 1567 2245
ApaLI G'TGCA_C
Cuts at: 2582 2582
Size: 3812
ApoI r'AATT_y
Cuts at: 364 816 3165 3421 364
Size: 452 2349 256 755
Fragments arranged by size:
2349 755 452 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3812
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
AvaI C'yCGr_G
Cuts at: 832 832
Size: 3812
BamHI G'GATC_C
Cuts at: 621 837 621
Size: 216 3596
BanI G'GyrC_C
Cuts at: 591 828 1242 1490 1525 591
Size: 237 414 248 35 2878
Fragments arranged by size:
2878 414 248 237 35
BanII G_rGCy'C
Cuts at: 296 616 826 1856 296
Size: 320 210 1030 2252
Fragments arranged by size:
2252 1030 320 210
BbeI G_GCGC'C
Cuts at: 1246 1494 1246
Size: 248 3564
BbsI GAAGACnn'nnnn_
Cuts at: 589 589
Size: 3812
BciVI GTATCCnnnnn_n'
Cuts at: 1710 2471 3812 1710
Size: 761 1341 1710
Fragments arranged by size:
1710 1341 761
BclI T'GATC_A
Cuts at: 1332 1332
Size: 3812
BglI GCCn_nnn'nGGC
Cuts at: 112 121 112
Size: 9 3803
BglII A'GATC_T
Cuts at: 1327 3364 1327
Size: 2037 1775
BlpI GC'TnA_GC
Cuts at: 924 924
Size: 3812
Bme1580I G_kGCm'C
Cuts at: 1437 1530 2586 1437
Size: 93 1056 2663
Fragments arranged by size:
2663 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1024 1105 1440 3584 1024
Size: 81 335 2144 1252
Fragments arranged by size:
2144 1252 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 310 2179 310
Size: 1869 1943
Bpu10I CC'TnA_GC
Cuts at: 520 520
Size: 3812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 988 1867 2359 2657 280
Size: 708 879 492 298 1435
Fragments arranged by size:
1435 879 708 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3516 3516
Size: 3812
BsaAI yAC'GTr
Cuts at: 1165 1165
Size: 3812
BsaBI GATnn'nnATC
Cuts at: 6 1326 3363 6
Size: 1320 2037 455
Fragments arranged by size:
2037 1320 455
BsaHI Gr'CG_yC
Cuts at: 1243 1491 2193 3689 1243
Size: 248 702 1496 1366
Fragments arranged by size:
1496 1366 702 248
BsaWI w'CCGG_w
Cuts at: 1001 1522 2200 2474 2621 3032 1001
Size: 521 678 274 147 411 1781
Fragments arranged by size:
1781 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 751 325
Size: 426 3386
BseYI C'CCAG_C
Cuts at: 1237 1995 2572 3068 1237
Size: 758 577 496 1981
Fragments arranged by size:
1981 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1354 1400 2608 104
Size: 1250 46 1208 1308
Fragments arranged by size:
1308 1250 1208 46
BsiHKAI G_wGCw'C
Cuts at: 296 465 564 573 672 826 1604 1794 2586
Size: 169 99 9 99 154 778 190 792
Cuts at: 2586 296
Size: 1522
Fragments arranged by size:
1522 792 778 190 169 154 99 99
9
Bsp1286I G_dGCh'C
Cuts at: 296 465 564 573 616 672 826 1437 1530
Size: 169 99 9 43 56 154 611 93
Cuts at: 1530 1604 1794 1856 2586 296
Size: 74 190 62 730 1522
Fragments arranged by size:
1522 730 611 190 169 154 99 93
74 62 56 43 9
BspEI T'CCGG_A
Cuts at: 3032 3032
Size: 3812
BspHI T'CATG_A
Cuts at: 2988 2988
Size: 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 652 862 1076 1378 1759 652
Size: 210 214 302 381 2705
Fragments arranged by size:
2705 381 302 214 210
BsrBI CCG'CTC
Cuts at: 50 2104 2158 3642 3807 50
Size: 2054 54 1484 165 55
Fragments arranged by size:
2054 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1724 1724
Size: 3812
BsrFI r'CCGG_y
Cuts at: 1810 1991 2200 1810
Size: 181 209 3422
Fragments arranged by size:
3422 209 181
BssSI C'ACGA_G
Cuts at: 2083 2441 2083
Size: 358 3454
BstAPI GCAn_nnn'nTGC
Cuts at: 568 1189 568
Size: 621 3191
BstBI TT'CG_AA
Cuts at: 2173 2173
Size: 3812
BstXI CCAn_nnnn'nTGG
Cuts at: 534 534
Size: 3812
BstYI r'GATC_y
Cuts at: 1 330 621 837 1304 1327 1662 1908 2920
Size: 329 291 216 467 23 335 246 1012
Cuts at: 2920 3006 3018 3364 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 329 291 246
216 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 714 714
Size: 3812
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1762 1943 2106 1762
Size: 181 163 3468
Fragments arranged by size:
3468 181 163
BtsI GCAGTG_nn'
Cuts at: 1055 1055
Size: 3812
DraI TTT'AAA
Cuts at: 811 811
Size: 3812
DraIII CAC_nnn'GTG
Cuts at: 568 774 568
Size: 206 3606
DrdI GACnn_nn'nnGTC
Cuts at: 1518 2376 1518
Size: 858 2954
EaeI y'GGCC_r
Cuts at: 113 1397 1571 1962 1989 3334 113
Size: 1284 174 391 27 1345 591
Fragments arranged by size:
1345 1284 591 391 174 27
EagI C'GGCC_G
Cuts at: 1397 1397
Size: 3812
EarI CTCTTCn'nnn_
Cuts at: 284 453 578 1835 2045 284
Size: 169 125 1257 210 2051
Fragments arranged by size:
2051 1257 210 169 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2020 2330 2476 3622 2020
Size: 310 146 1146 2210
Fragments arranged by size:
2210 1146 310 146
EcoICRI GAG'CTC
Cuts at: 294 824 294
Size: 530 3282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1637 2069 2816 3084 689
Size: 948 432 747 268 1417
Fragments arranged by size:
1417 948 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 258 641 951 258
Size: 383 310 3119
Fragments arranged by size:
3119 383 310
EcoRI G'AATT_C
Cuts at: 816 816
Size: 3812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1831 1831
Size: 3812
FspI TGC'GCA
Cuts at: 1593 1593
Size: 3812
HaeII r_GCGC'y
Cuts at: 1246 1494 2516 3495 1246
Size: 248 1022 979 1563
Fragments arranged by size:
1563 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 315 347 1654 1684 1686 1716 315
Size: 32 1307 30 2 30 2411
Fragments arranged by size:
2411 1307 32 30 30 2
HincII GTy'rAC
Cuts at: 851 851
Size: 3812
HindIII A'AGCT_T
Cuts at: 445 445
Size: 3812
KasI G'GCGC_C
Cuts at: 1242 1490 1242
Size: 248 3564
KpnI G_GTAC'C
Cuts at: 832 832
Size: 3812
MluI A'CGCG_T
Cuts at: 3245 3245
Size: 3812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2483 2667 2483
Size: 184 3628
MscI TGG'CCA
Cuts at: 1573 1573
Size: 3812
MslI CAynn'nnrTG
Cuts at: 144 635 1801 1928 144
Size: 491 1166 127 2028
Fragments arranged by size:
2028 1166 491 127
MspA1I CmG'CkG
Cuts at: 923 1237 1597 2610 2855 923
Size: 314 360 1013 245 1880
Fragments arranged by size:
1880 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1993 1993
Size: 3812
NarI GG'CG_CC
Cuts at: 1243 1491 1243
Size: 248 3564
NgoMIV G'CCGG_C
Cuts at: 1991 1991
Size: 3812
NspI r_CATG'y
Cuts at: 865 1365 2272 865
Size: 500 907 2405
Fragments arranged by size:
2405 907 500
PciI A'CATG_T
Cuts at: 2268 2268
Size: 3812
PmeI GTTT'AAAC
Cuts at: 811 811
Size: 3812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 503 535 2951 2983 503
Size: 32 2416 32 1332
Fragments arranged by size:
2416 1332 32 32
PpuMI rG'GwC_Cy
Cuts at: 258 641 258
Size: 383 3429
PstI C_TGCA'G
Cuts at: 859 1544 859
Size: 685 3127
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3812
PvuII CAG'CTG
Cuts at: 1237 1597 1237
Size: 360 3452
RsrII CG'GwC_CG
Cuts at: 2007 2007
Size: 3812
SacI G_AGCT'C
Cuts at: 296 826 296
Size: 530 3282
SalI G'TCGA_C
Cuts at: 849 849
Size: 3812
SapI GCTCTTCn'nnn_
Cuts at: 284 453 578 1835 2045 284
Size: 169 125 1257 210 2051
Fragments arranged by size:
2051 1257 210 169 125
SbfI CC_TGCA'GG
Cuts at: 859 859
Size: 3812
ScaI AGT'ACT
Cuts at: 3329 3329
Size: 3812
SfcI C'TryA_G
Cuts at: 33 855 1540 2533 2724 33
Size: 822 685 993 191 1121
Fragments arranged by size:
1121 993 822 685 191
SfoI GGC'GCC
Cuts at: 1244 1492 1244
Size: 248 3564
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
SmaI CCC'GGG
Cuts at: 834 834
Size: 3812
SmlI C'TyrA_G
Cuts at: 295 967 1882 2374 2636 295
Size: 672 915 492 262 1471
Fragments arranged by size:
1471 915 672 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3812
SphI G_CATG'C
Cuts at: 865 1365 865
Size: 500 3312
StuI AGG'CCT
Cuts at: 712 719 712
Size: 7 3805
StyI C'CwwG_G
Cuts at: 946 1300 946
Size: 354 3458
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 766 1395 1528 1987 2196 766
Size: 629 133 459 209 2382
Fragments arranged by size:
2382 629 459 209 133
TatI w'GTAC_w
Cuts at: 3327 3327
Size: 3812
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 3780
Tth111I GACn'n_nGTC
Cuts at: 1609 1609
Size: 3812
XbaI T'CTAG_A
Cuts at: 843 843
Size: 3812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1307 1307
Size: 3812
XmaI C'CCGG_G
Cuts at: 832 832
Size: 3812
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I BtgI ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI