Restriction map for
pF1KE0438
(Circular) (Six-base) MAPSORT of: pF1KE0438.seq Check: 3040 from: 1 to: 4097
pF1KE0438 4097 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 481 774 481
Size: 293 3804
AatII G_ACGT'C
Cuts at: 851 851
Size: 4097
AccI GT'mk_AC
Cuts at: 709 1135 709
Size: 426 3671
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1922 2354 3101 3369 982
Size: 940 432 747 268 1710
Fragments arranged by size:
1710 940 747 432 268
AflIII A'CryG_T
Cuts at: 1449 2553 3530 1449
Size: 1104 977 2016
Fragments arranged by size:
2016 1104 977
AgeI A'CCGG_T
Cuts at: 114 2485 114
Size: 2371 1726
AlwNI CAG_nnn'CTG
Cuts at: 904 1402 2969 904
Size: 498 1567 2032
Fragments arranged by size:
2032 1567 498
ApaI G_GGCC'C
Cuts at: 264 264
Size: 4097
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 688 1101 3450 3706 688
Size: 413 2349 256 1079
Fragments arranged by size:
2349 1079 413 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 179 1117 179
Size: 938 3159
BamHI G'GATC_C
Cuts at: 305 398 1122 305
Size: 93 724 3280
Fragments arranged by size:
3280 724 93
BanI G'GyrC_C
Cuts at: 659 1113 1527 1775 1810 659
Size: 454 414 248 35 2946
Fragments arranged by size:
2946 454 414 248 35
BanII G_rGCy'C
Cuts at: 224 264 925 1111 2141 224
Size: 40 661 186 1030 2180
Fragments arranged by size:
2180 1030 661 186 40
BbeI G_GCGC'C
Cuts at: 663 1531 1779 663
Size: 868 248 2981
Fragments arranged by size:
2981 868 248
BciVI GTATCCnnnnn_n'
Cuts at: 143 1995 2756 4097 143
Size: 1852 761 1341 143
Fragments arranged by size:
1852 1341 761 143
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglI GCCn_nnn'nGGC
Cuts at: 184 540 184
Size: 356 3741
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 1209 1209
Size: 4097
Bme1580I G_kGCm'C
Cuts at: 264 1722 1815 2871 264
Size: 1458 93 1056 1490
Fragments arranged by size:
1490 1458 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1309 1390 1725 3869 1309
Size: 81 335 2144 1537
Fragments arranged by size:
2144 1537 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4097
Bpu10I CC'TnA_GC
Cuts at: 716 716
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1273 2152 2644 2942 1069
Size: 204 879 492 298 2224
Fragments arranged by size:
2224 879 492 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 105 3801 105
Size: 3696 401
BsaAI yAC'GTr
Cuts at: 294 1450 294
Size: 1156 2941
BsaBI GATnn'nnATC
Cuts at: 6 1611 3648 6
Size: 1605 2037 455
Fragments arranged by size:
2037 1605 455
BsaHI Gr'CG_yC
Cuts at: 618 660 848 1528 1776 2478 3974 618
Size: 42 188 680 248 702 1496 741
Fragments arranged by size:
1496 741 702 680 248 188 42
BsaWI w'CCGG_w
Cuts at: 114 1286 1807 2485 2759 2906 3317 114
Size: 1172 521 678 274 147 411 894
Fragments arranged by size:
1172 894 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 271
Size: 4097
BseYI C'CCAG_C
Cuts at: 588 1522 2280 2857 3353 588
Size: 934 758 577 496 1332
Fragments arranged by size:
1332 934 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 571 1111 1889 2079 2871 571
Size: 540 778 190 792 1797
Fragments arranged by size:
1797 792 778 540 190
Bsp1286I G_dGCh'C
Cuts at: 224 264 571 925 1111 1722 1815 1889 2079
Size: 40 307 354 186 611 93 74 190
Cuts at: 2079 2141 2871 224
Size: 62 730 1450
Fragments arranged by size:
1450 730 611 354 307 190 186 93
74 62 40
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 125 3273 125
Size: 3148 949
BspMI ACCTGCnnnn'nnnn_
Cuts at: 481 774 1147 1361 1663 2044 481
Size: 293 373 214 302 381 2534
Fragments arranged by size:
2534 381 373 302 293 214
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 608 2009 608
Size: 1401 2696
BsrFI r'CCGG_y
Cuts at: 114 479 2095 2276 2485 114
Size: 365 1616 181 209 1726
Fragments arranged by size:
1726 1616 365 209 181
BsrGI T'GTAC_A
Cuts at: 650 650
Size: 4097
BssSI C'ACGA_G
Cuts at: 525 2368 2726 525
Size: 1843 358 1896
Fragments arranged by size:
1896 1843 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 354 354
Size: 4097
BstYI r'GATC_y
Cuts at: 1 305 398 1122 1589 1612 1947 2193 3205
Size: 304 93 724 467 23 335 246 1012
Cuts at: 3205 3291 3303 3649 1
Size: 86 12 346 449
Fragments arranged by size:
1012 724 467 449 346 335 304 246
93 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 674 674
Size: 4097
BtgI C'CryG_G
Cuts at: 264 264
Size: 4097
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2047 2228 2391 2047
Size: 181 163 3753
Fragments arranged by size:
3753 181 163
BtsI GCAGTG_nn'
Cuts at: 211 931 999 1340 211
Size: 720 68 341 2968
Fragments arranged by size:
2968 720 341 68
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 375 410 375
Size: 35 4062
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 881 1682 1856 2247 2274 3619 881
Size: 801 174 391 27 1345 1359
Fragments arranged by size:
1359 1345 801 391 174 27
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2305 2615 2761 3907 2305
Size: 310 146 1146 2495
Fragments arranged by size:
2495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1109 1109
Size: 4097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1922 2354 3101 3369 982
Size: 940 432 747 268 1710
Fragments arranged by size:
1710 940 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 871 871
Size: 4097
EcoO109I rG'GnC_Cy
Cuts at: 260 1236 260
Size: 976 3121
EcoRI G'AATT_C
Cuts at: 688 1101 688
Size: 413 3684
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 813 2116 813
Size: 1303 2794
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 663 1531 1779 2801 3780 663
Size: 868 248 1022 979 980
Fragments arranged by size:
1022 980 979 868 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 691 723 1939 1969 1971 2001 691
Size: 32 1216 30 2 30 2787
Fragments arranged by size:
2787 1216 32 30 30 2
HincII GTy'rAC
Cuts at: 1136 1136
Size: 4097
KasI G'GCGC_C
Cuts at: 659 1527 1775 659
Size: 868 248 2981
Fragments arranged by size:
2981 868 248
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 311 2768 2952 311
Size: 2457 184 1456
Fragments arranged by size:
2457 1456 184
MscI TGG'CCA
Cuts at: 883 1858 883
Size: 975 3122
MslI CAynn'nnrTG
Cuts at: 130 352 745 2086 2213 130
Size: 222 393 1341 127 2014
Fragments arranged by size:
2014 1341 393 222 127
MspA1I CmG'CkG
Cuts at: 685 820 901 1208 1522 1882 2895 3140 685
Size: 135 81 307 314 360 1013 245 1642
Fragments arranged by size:
1642 1013 360 314 307 245 135 81
NaeI GCC'GGC
Cuts at: 2278 2278
Size: 4097
NarI GG'CG_CC
Cuts at: 660 1528 1776 660
Size: 868 248 2981
Fragments arranged by size:
2981 868 248
NgoMIV G'CCGG_C
Cuts at: 2276 2276
Size: 4097
NspI r_CATG'y
Cuts at: 1150 1650 2557 1150
Size: 500 907 2690
Fragments arranged by size:
2690 907 500
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PflMI CCAn_nnn'nTGG
Cuts at: 879 879
Size: 4097
PfoI T'CCnGG_A
Cuts at: 308 308
Size: 4097
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PmlI CAC'GTG
Cuts at: 294 294
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PspOMI G'GGCC_C
Cuts at: 260 260
Size: 4097
PstI C_TGCA'G
Cuts at: 321 1144 1829 321
Size: 823 685 2589
Fragments arranged by size:
2589 823 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 820 901 1522 1882 820
Size: 81 621 360 3035
Fragments arranged by size:
3035 621 360 81
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 1111 1111
Size: 4097
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SfcI C'TryA_G
Cuts at: 33 317 1140 1825 2818 3009 33
Size: 284 823 685 993 191 1121
Fragments arranged by size:
1121 993 823 685 284 191
SfoI GGC'GCC
Cuts at: 661 1529 1777 661
Size: 868 248 2981
Fragments arranged by size:
2981 868 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 181 1119 181
Size: 938 3159
SmlI C'TyrA_G
Cuts at: 1084 1252 2167 2659 2921 1084
Size: 168 915 492 262 2260
Fragments arranged by size:
2260 915 492 262 168
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
SrfI GCCC'GGGC
Cuts at: 181 181
Size: 4097
StuI AGG'CCT
Cuts at: 672 877 672
Size: 205 3892
StyI C'CwwG_G
Cuts at: 233 878 1231 1585 233
Size: 645 353 354 2745
Fragments arranged by size:
2745 645 354 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1015 1680 1813 2272 2481 1015
Size: 665 133 459 209 2631
Fragments arranged by size:
2631 665 459 209 133
TatI w'GTAC_w
Cuts at: 650 3612 650
Size: 2962 1135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 899 931 3614 3646 899
Size: 32 2683 32 1350
Fragments arranged by size:
2683 1350 32 32
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1026 1592 1026
Size: 566 3531
XmaI C'CCGG_G
Cuts at: 179 1117 179
Size: 938 3159
XmnI GAAnn'nnTTC
Cuts at: 133 628 133
Size: 495 3602
ZraI GAC'GTC
Cuts at: 849 849
Size: 4097
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BpmI
BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SspI SwaI XhoI