Restriction map for
pF1KE0430
(Circular) (Six-base) MAPSORT of: pF1KE0430.seq Check: 2598 from: 1 to: 4364
pF1KE0430 4364 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 990 990
Size: 4364
AccI GT'mk_AC
Cuts at: 839 1402 839
Size: 563 3801
Acc65I G'GTAC_C
Cuts at: 1380 1380
Size: 4364
AclI AA'CG_TT
Cuts at: 4204 4204
Size: 4364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 2189 2621 3368 3636 544
Size: 1645 432 747 268 1272
Fragments arranged by size:
1645 1272 747 432 268
AflIII A'CryG_T
Cuts at: 1716 2820 3797 1716
Size: 1104 977 2283
Fragments arranged by size:
2283 1104 977
AgeI A'CCGG_T
Cuts at: 2752 2752
Size: 4364
AlwNI CAG_nnn'CTG
Cuts at: 507 778 1669 3236 507
Size: 271 891 1567 1635
Fragments arranged by size:
1635 1567 891 271
ApaI G_GGCC'C
Cuts at: 489 557 819 489
Size: 68 262 4034
Fragments arranged by size:
4034 262 68
ApaLI G'TGCA_C
Cuts at: 1235 3134 1235
Size: 1899 2465
ApoI r'AATT_y
Cuts at: 342 1368 3717 3973 342
Size: 1026 2349 256 733
Fragments arranged by size:
2349 1026 733 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4364
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
AvaI C'yCGr_G
Cuts at: 1384 1384
Size: 4364
AvrII C'CTAG_G
Cuts at: 942 942
Size: 4364
BamHI G'GATC_C
Cuts at: 1389 1389
Size: 4364
BanI G'GyrC_C
Cuts at: 544 1380 1794 2042 2077 544
Size: 836 414 248 35 2831
Fragments arranged by size:
2831 836 414 248 35
BanII G_rGCy'C
Cuts at: 489 557 819 910 1009 1378 2408 489
Size: 68 262 91 99 369 1030 2445
Fragments arranged by size:
2445 1030 369 262 99 91 68
BbeI G_GCGC'C
Cuts at: 1798 2046 1798
Size: 248 4116
BbsI GAAGACnn'nnnn_
Cuts at: 209 1193 209
Size: 984 3380
BbvCI CC'TCA_GC
Cuts at: 157 230 157
Size: 73 4291
BciVI GTATCCnnnnn_n'
Cuts at: 2262 3023 4364 2262
Size: 761 1341 2262
Fragments arranged by size:
2262 1341 761
BclI T'GATC_A
Cuts at: 1884 1884
Size: 4364
BglI GCCn_nnn'nGGC
Cuts at: 542 662 692 1249 542
Size: 120 30 557 3657
Fragments arranged by size:
3657 557 120 30
BglII A'GATC_T
Cuts at: 1879 3916 1879
Size: 2037 2327
BlpI GC'TnA_GC
Cuts at: 1476 1476
Size: 4364
Bme1580I G_kGCm'C
Cuts at: 489 557 647 765 819 1239 1989 2082 3138
Size: 68 90 118 54 420 750 93 1056
Cuts at: 3138 489
Size: 1715
Fragments arranged by size:
1715 1056 750 420 118 93 90 68
54
BmrI ACTGGGnnnn_n'
Cuts at: 207 1576 1657 1992 4136 207
Size: 1369 81 335 2144 435
Fragments arranged by size:
2144 1369 435 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 4364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 307 409 454 1366 178
Size: 129 102 45 912 3176
Fragments arranged by size:
3176 912 129 102 45
Bpu10I CC'TnA_GC
Cuts at: 157 230 771 1256 157
Size: 73 541 485 3265
Fragments arranged by size:
3265 541 485 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1540 2419 2911 3209 814
Size: 726 879 492 298 1969
Fragments arranged by size:
1969 879 726 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 361 4068 361
Size: 3707 657
BsaAI yAC'GTr
Cuts at: 1717 1717
Size: 4364
BsaBI GATnn'nnATC
Cuts at: 6 1878 3915 6
Size: 1872 2037 455
Fragments arranged by size:
2037 1872 455
BsaHI Gr'CG_yC
Cuts at: 1795 2043 2745 4241 1795
Size: 248 702 1496 1918
Fragments arranged by size:
1918 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1553 2074 2752 3026 3173 3584 1553
Size: 521 678 274 147 411 2333
Fragments arranged by size:
2333 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 382 412 448 478 382
Size: 30 36 30 4268
Fragments arranged by size:
4268 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 460 460
Size: 4364
BseYI C'CCAG_C
Cuts at: 138 812 1789 2547 3124 3620 138
Size: 674 977 758 577 496 882
Fragments arranged by size:
977 882 758 674 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 420
Size: 4364
BsiEI CG_ry'CG
Cuts at: 104 1906 1952 3160 104
Size: 1802 46 1208 1308
Fragments arranged by size:
1802 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 149 514 1239 1378 2156 2346 3138 149
Size: 365 725 139 778 190 792 1375
Fragments arranged by size:
1375 792 778 725 365 190 139
Bsp1286I G_dGCh'C
Cuts at: 149 489 514 557 647 765 819 910 1009
Size: 340 25 43 90 118 54 91 99
Cuts at: 1009 1239 1378 1989 2082 2156 2346 2408 3138
Size: 230 139 611 93 74 190 62 730
Cuts at: 3138 149
Size: 1375
Fragments arranged by size:
1375 730 611 340 230 190 139 118
99 93 91 90 74 62 54 43
25
BspEI T'CCGG_A
Cuts at: 3584 3584
Size: 4364
BspHI T'CATG_A
Cuts at: 3540 3540
Size: 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 259 990 1414 1628 1930 2311 259
Size: 731 424 214 302 381 2312
Fragments arranged by size:
2312 731 424 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2656 2710 4194 4359 50
Size: 2606 54 1484 165 55
Fragments arranged by size:
2606 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 805 2276 805
Size: 1471 2893
BsrFI r'CCGG_y
Cuts at: 661 2362 2543 2752 661
Size: 1701 181 209 2273
Fragments arranged by size:
2273 1701 209 181
BssSI C'ACGA_G
Cuts at: 629 2635 2993 629
Size: 2006 358 2000
Fragments arranged by size:
2006 2000 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1741 1741
Size: 4364
BstBI TT'CG_AA
Cuts at: 2725 2725
Size: 4364
BstXI CCAn_nnnn'nTGG
Cuts at: 732 732
Size: 4364
BstYI r'GATC_y
Cuts at: 1 1148 1389 1856 1879 2214 2460 3472 3558
Size: 1147 241 467 23 335 246 1012 86
Cuts at: 3558 3570 3916 1
Size: 12 346 449
Fragments arranged by size:
1147 1012 467 449 346 335 246 241
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 779 779
Size: 4364
BtgI C'CryG_G
Cuts at: 107 115 107
Size: 8 4356
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2314 2495 2658 2314
Size: 181 163 4020
Fragments arranged by size:
4020 181 163
BtsI GCAGTG_nn'
Cuts at: 323 616 1072 1338 1607 323
Size: 293 456 266 269 3080
Fragments arranged by size:
3080 456 293 269 266
DraI TTT'AAA
Cuts at: 1363 1363
Size: 4364
DrdI GACnn_nn'nnGTC
Cuts at: 2070 2928 2070
Size: 858 3506
EaeI y'GGCC_r
Cuts at: 684 1241 1301 1949 2123 2514 2541 3886 684
Size: 557 60 648 174 391 27 1345 1162
Fragments arranged by size:
1345 1162 648 557 391 174 60 27
EagI C'GGCC_G
Cuts at: 1949 1949
Size: 4364
EarI CTCTTCn'nnn_
Cuts at: 169 332 502 2387 2597 169
Size: 163 170 1885 210 1936
Fragments arranged by size:
1936 1885 210 170 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2572 2882 3028 4174 2572
Size: 310 146 1146 2762
Fragments arranged by size:
2762 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1376 1376
Size: 4364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 307 409 454 544 1366 2189 2621 3368
Size: 129 102 45 90 822 823 432 747
Cuts at: 3368 3636 178
Size: 268 906
Fragments arranged by size:
906 823 822 747 432 268 129 102
90 45
EcoNI CCTnn'n_nnAGG
Cuts at: 379 379
Size: 4364
EcoO109I rG'GnC_Cy
Cuts at: 129 153 181 282 485 486 554 590 775
Size: 24 28 101 203 1 68 36 185
Cuts at: 775 816 1503 129
Size: 41 687 2990
Fragments arranged by size:
2990 687 203 185 101 68 41 36
28 24 1
EcoRI G'AATT_C
Cuts at: 1368 1368
Size: 4364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 429 1281 2383 429
Size: 852 1102 2410
Fragments arranged by size:
2410 1102 852
FspI TGC'GCA
Cuts at: 2145 2145
Size: 4364
HaeII r_GCGC'y
Cuts at: 1798 2046 3068 4047 1798
Size: 248 1022 979 2115
Fragments arranged by size:
2115 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 331 363 2206 2236 2238 2268 331
Size: 32 1843 30 2 30 2427
Fragments arranged by size:
2427 1843 32 30 30 2
HincII GTy'rAC
Cuts at: 267 1403 267
Size: 1136 3228
KasI G'GCGC_C
Cuts at: 1794 2042 1794
Size: 248 4116
KpnI G_GTAC'C
Cuts at: 1384 1384
Size: 4364
MluI A'CGCG_T
Cuts at: 3797 3797
Size: 4364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3035 3219 3035
Size: 184 4180
MscI TGG'CCA
Cuts at: 686 1243 2125 686
Size: 557 882 2925
Fragments arranged by size:
2925 882 557
MslI CAynn'nnrTG
Cuts at: 1135 2353 2480 1135
Size: 1218 127 3019
Fragments arranged by size:
3019 1218 127
MspA1I CmG'CkG
Cuts at: 142 641 1475 1789 2149 3162 3407 142
Size: 499 834 314 360 1013 245 1099
Fragments arranged by size:
1099 1013 834 499 360 314 245
NaeI GCC'GGC
Cuts at: 663 2545 663
Size: 1882 2482
NarI GG'CG_CC
Cuts at: 1795 2043 1795
Size: 248 4116
NcoI C'CATG_G
Cuts at: 107 115 107
Size: 8 4356
NgoMIV G'CCGG_C
Cuts at: 661 2543 661
Size: 1882 2482
NspI r_CATG'y
Cuts at: 1417 1917 2824 1417
Size: 500 907 2957
Fragments arranged by size:
2957 907 500
PciI A'CATG_T
Cuts at: 2820 2820
Size: 4364
PfoI T'CCnGG_A
Cuts at: 299 1050 299
Size: 751 3613
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 4332
PpuMI rG'GwC_Cy
Cuts at: 181 775 181
Size: 594 3770
PspOMI G'GGCC_C
Cuts at: 485 553 815 485
Size: 68 262 4034
Fragments arranged by size:
4034 262 68
PstI C_TGCA'G
Cuts at: 1208 1411 2096 1208
Size: 203 685 3476
Fragments arranged by size:
3476 685 203
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4364
PvuII CAG'CTG
Cuts at: 142 1789 2149 142
Size: 1647 360 2357
Fragments arranged by size:
2357 1647 360
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4364
SacI G_AGCT'C
Cuts at: 1378 1378
Size: 4364
SalI G'TCGA_C
Cuts at: 1401 1401
Size: 4364
SapI GCTCTTCn'nnn_
Cuts at: 2387 2597 2387
Size: 210 4154
SbfI CC_TGCA'GG
Cuts at: 1411 1411
Size: 4364
ScaI AGT'ACT
Cuts at: 3881 3881
Size: 4364
SfcI C'TryA_G
Cuts at: 33 1204 1407 2092 3085 3276 33
Size: 1171 203 685 993 191 1121
Fragments arranged by size:
1171 1121 993 685 203 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 692 692
Size: 4364
SfoI GGC'GCC
Cuts at: 1796 2044 1796
Size: 248 4116
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
SmaI CCC'GGG
Cuts at: 1386 1386
Size: 4364
SmlI C'TyrA_G
Cuts at: 829 1519 2434 2926 3188 829
Size: 690 915 492 262 2005
Fragments arranged by size:
2005 915 690 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4364
SphI G_CATG'C
Cuts at: 1417 1917 1417
Size: 500 3864
StuI AGG'CCT
Cuts at: 679 937 679
Size: 258 4106
StyI C'CwwG_G
Cuts at: 107 115 696 932 942 1498 1852 107
Size: 8 581 236 10 556 354 2619
Fragments arranged by size:
2619 581 556 354 236 10 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1947 2080 2539 2748 1947
Size: 133 459 209 3563
Fragments arranged by size:
3563 459 209 133
TatI w'GTAC_w
Cuts at: 3879 3879
Size: 4364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 382 414 448 480 3881 3913 382
Size: 32 34 32 3401 32 833
Fragments arranged by size:
3401 833 34 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2161 2161
Size: 4364
XbaI T'CTAG_A
Cuts at: 1395 1395
Size: 4364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 146 185 694 1859 146
Size: 39 509 1165 2651
Fragments arranged by size:
2651 1165 509 39
XmaI C'CCGG_G
Cuts at: 1384 1384
Size: 4364
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI