Restriction map for
pF1KE0426
(Circular) (Six-base) MAPSORT of: pF1KE0426.seq Check: 6600 from: 1 to: 4262
pF1KE0426 4262 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 396 396
Size: 4262
AatII G_ACGT'C
Cuts at: 998 998
Size: 4262
AccI GT'mk_AC
Cuts at: 238 1300 238
Size: 1062 3200
Acc65I G'GTAC_C
Cuts at: 1278 1278
Size: 4262
AclI AA'CG_TT
Cuts at: 4102 4102
Size: 4262
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 452 722 2087 2519 3266 3534 452
Size: 270 1365 432 747 268 1180
Fragments arranged by size:
1365 1180 747 432 270 268
AflIII A'CryG_T
Cuts at: 1614 2718 3695 1614
Size: 1104 977 2181
Fragments arranged by size:
2181 1104 977
AgeI A'CCGG_T
Cuts at: 2650 2650
Size: 4262
AhdI GACnn_n'nnGTC
Cuts at: 462 462
Size: 4262
AleI CACnn'nnGTG
Cuts at: 598 790 598
Size: 192 4070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 808 1052 808
Size: 244 4018
AlwNI CAG_nnn'CTG
Cuts at: 1567 3134 1567
Size: 1567 2695
ApaI G_GGCC'C
Cuts at: 447 447
Size: 4262
ApaLI G'TGCA_C
Cuts at: 3032 3032
Size: 4262
ApoI r'AATT_y
Cuts at: 760 883 935 1266 3615 3871 760
Size: 123 52 331 2349 256 1151
Fragments arranged by size:
2349 1151 331 256 123 52
AseI AT'TA_AT
Cuts at: 20 20
Size: 4262
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4262
AvaI C'yCGr_G
Cuts at: 748 1282 748
Size: 534 3728
BamHI G'GATC_C
Cuts at: 770 1287 770
Size: 517 3745
BanI G'GyrC_C
Cuts at: 592 1133 1278 1692 1940 1975 592
Size: 541 145 414 248 35 2879
Fragments arranged by size:
2879 541 414 248 145 35
BanII G_rGCy'C
Cuts at: 128 175 447 907 1276 2306 128
Size: 47 272 460 369 1030 2084
Fragments arranged by size:
2084 1030 460 369 272 47
BbeI G_GCGC'C
Cuts at: 1696 1944 1696
Size: 248 4014
BbsI GAAGACnn'nnnn_
Cuts at: 458 458
Size: 4262
BbvCI CC'TCA_GC
Cuts at: 424 424
Size: 4262
BciVI GTATCCnnnnn_n'
Cuts at: 2160 2921 4262 2160
Size: 761 1341 2160
Fragments arranged by size:
2160 1341 761
BclI T'GATC_A
Cuts at: 1782 1782
Size: 4262
BglII A'GATC_T
Cuts at: 1777 3814 1777
Size: 2037 2225
BlpI GC'TnA_GC
Cuts at: 914 1374 914
Size: 460 3802
Bme1580I G_kGCm'C
Cuts at: 186 447 797 1136 1887 1980 3036 186
Size: 261 350 339 751 93 1056 1412
Fragments arranged by size:
1412 1056 751 350 339 261 93
BmrI ACTGGGnnnn_n'
Cuts at: 228 303 1474 1555 1890 4034 228
Size: 75 1171 81 335 2144 456
Fragments arranged by size:
2144 1171 456 335 81 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2629 2629
Size: 4262
Bpu10I CC'TnA_GC
Cuts at: 424 424
Size: 4262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1438 2317 2809 3107 1438
Size: 879 492 298 2593
Fragments arranged by size:
2593 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1000 3966 1000
Size: 2966 1296
BsaAI yAC'GTr
Cuts at: 1615 1615
Size: 4262
BsaBI GATnn'nnATC
Cuts at: 6 1776 3813 6
Size: 1770 2037 455
Fragments arranged by size:
2037 1770 455
BsaHI Gr'CG_yC
Cuts at: 995 1693 1941 2643 4139 995
Size: 698 248 702 1496 1118
Fragments arranged by size:
1496 1118 702 698 248
BsaWI w'CCGG_w
Cuts at: 1451 1972 2650 2924 3071 3482 1451
Size: 521 678 274 147 411 2231
Fragments arranged by size:
2231 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 118 1184 118
Size: 1066 3196
BseYI C'CCAG_C
Cuts at: 1040 1687 2445 3022 3518 1040
Size: 647 758 577 496 1784
Fragments arranged by size:
1784 758 647 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 570 570
Size: 4262
BsiEI CG_ry'CG
Cuts at: 104 1804 1850 3058 104
Size: 1700 46 1208 1308
Fragments arranged by size:
1700 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1276 2054 2244 3036 1276
Size: 778 190 792 2502
Fragments arranged by size:
2502 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 394 394
Size: 4262
Bsp1286I G_dGCh'C
Cuts at: 128 175 186 447 797 907 1136 1276 1887
Size: 47 11 261 350 110 229 140 611
Cuts at: 1887 1980 2054 2244 2306 3036 128
Size: 93 74 190 62 730 1354
Fragments arranged by size:
1354 730 611 350 261 229 190 140
110 93 74 62 47 11
BspEI T'CCGG_A
Cuts at: 3482 3482
Size: 4262
BspHI T'CATG_A
Cuts at: 3438 3438
Size: 4262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 396 503 1312 1526 1828 2209 396
Size: 107 809 214 302 381 2449
Fragments arranged by size:
2449 809 381 302 214 107
BsrBI CCG'CTC
Cuts at: 50 2554 2608 4092 4257 50
Size: 2504 54 1484 165 55
Fragments arranged by size:
2504 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 822 1036 2174 822
Size: 214 1138 2910
Fragments arranged by size:
2910 1138 214
BsrFI r'CCGG_y
Cuts at: 589 2260 2441 2650 589
Size: 1671 181 209 2201
Fragments arranged by size:
2201 1671 209 181
BssSI C'ACGA_G
Cuts at: 2533 2891 2533
Size: 358 3904
BstAPI GCAn_nnn'nTGC
Cuts at: 1639 1639
Size: 4262
BstBI TT'CG_AA
Cuts at: 2623 2623
Size: 4262
BstYI r'GATC_y
Cuts at: 1 770 1287 1754 1777 2112 2358 3370 3456
Size: 769 517 467 23 335 246 1012 86
Cuts at: 3456 3468 3814 1
Size: 12 346 449
Fragments arranged by size:
1012 769 517 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 578 107
Size: 471 3791
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1049 2212 2393 2556 1049
Size: 1163 181 163 2755
Fragments arranged by size:
2755 1163 181 163
BtsI GCAGTG_nn'
Cuts at: 328 630 1505 328
Size: 302 875 3085
Fragments arranged by size:
3085 875 302
DraI TTT'AAA
Cuts at: 705 1261 705
Size: 556 3706
DrdI GACnn_nn'nnGTC
Cuts at: 311 1968 2826 311
Size: 1657 858 1747
Fragments arranged by size:
1747 1657 858
EaeI y'GGCC_r
Cuts at: 1847 2021 2412 2439 3784 1847
Size: 174 391 27 1345 2325
Fragments arranged by size:
2325 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1847 1847
Size: 4262
EarI CTCTTCn'nnn_
Cuts at: 136 145 338 1078 2285 2495 136
Size: 9 193 740 1207 210 1903
Fragments arranged by size:
1903 1207 740 210 193 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2470 2780 2926 4072 2470
Size: 310 146 1146 2660
Fragments arranged by size:
2660 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1274 1274
Size: 4262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 452 722 2087 2519 3266 3534 452
Size: 270 1365 432 747 268 1180
Fragments arranged by size:
1365 1180 747 432 270 268
EcoO109I rG'GnC_Cy
Cuts at: 443 1401 443
Size: 958 3304
EcoRI G'AATT_C
Cuts at: 935 1266 935
Size: 331 3931
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 279 2281 279
Size: 2002 2260
FspI TGC'GCA
Cuts at: 2043 2043
Size: 4262
HaeII r_GCGC'y
Cuts at: 1696 1944 2966 3945 1696
Size: 248 1022 979 2013
Fragments arranged by size:
2013 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 448 480 2104 2134 2136 2166 448
Size: 32 1624 30 2 30 2544
Fragments arranged by size:
2544 1624 32 30 30 2
HincII GTy'rAC
Cuts at: 1301 1301
Size: 4262
HindIII A'AGCT_T
Cuts at: 673 673
Size: 4262
KasI G'GCGC_C
Cuts at: 1692 1940 1692
Size: 248 4014
KpnI G_GTAC'C
Cuts at: 1282 1282
Size: 4262
MluI A'CGCG_T
Cuts at: 3695 3695
Size: 4262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 784 2933 3117 784
Size: 2149 184 1929
Fragments arranged by size:
2149 1929 184
MscI TGG'CCA
Cuts at: 2023 2023
Size: 4262
MslI CAynn'nnrTG
Cuts at: 598 790 832 2251 2378 598
Size: 192 42 1419 127 2482
Fragments arranged by size:
2482 1419 192 127 42
MspA1I CmG'CkG
Cuts at: 625 921 1055 1373 1687 2047 3060 3305 625
Size: 296 134 318 314 360 1013 245 1582
Fragments arranged by size:
1582 1013 360 318 314 296 245 134
NaeI GCC'GGC
Cuts at: 2443 2443
Size: 4262
NarI GG'CG_CC
Cuts at: 1693 1941 1693
Size: 248 4014
NcoI C'CATG_G
Cuts at: 107 107
Size: 4262
NgoMIV G'CCGG_C
Cuts at: 2441 2441
Size: 4262
NspI r_CATG'y
Cuts at: 616 1315 1815 2722 616
Size: 699 500 907 2156
Fragments arranged by size:
2156 907 699 500
PciI A'CATG_T
Cuts at: 2718 2718
Size: 4262
PflMI CCAn_nnn'nTGG
Cuts at: 265 803 1143 265
Size: 538 340 3384
Fragments arranged by size:
3384 538 340
PfoI T'CCnGG_A
Cuts at: 854 854
Size: 4262
PmeI GTTT'AAAC
Cuts at: 1261 1261
Size: 4262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3401 3433 3401
Size: 32 4230
PspOMI G'GGCC_C
Cuts at: 443 443
Size: 4262
PstI C_TGCA'G
Cuts at: 1309 1994 1309
Size: 685 3577
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4262
PvuII CAG'CTG
Cuts at: 625 921 1687 2047 625
Size: 296 766 360 2840
Fragments arranged by size:
2840 766 360 296
RsrII CG'GwC_CG
Cuts at: 2457 2457
Size: 4262
SacI G_AGCT'C
Cuts at: 1276 1276
Size: 4262
SalI G'TCGA_C
Cuts at: 1299 1299
Size: 4262
SapI GCTCTTCn'nnn_
Cuts at: 2285 2495 2285
Size: 210 4052
SbfI CC_TGCA'GG
Cuts at: 1309 1309
Size: 4262
ScaI AGT'ACT
Cuts at: 3779 3779
Size: 4262
SfcI C'TryA_G
Cuts at: 33 1305 1990 2983 3174 33
Size: 1272 685 993 191 1121
Fragments arranged by size:
1272 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1694 1942 1694
Size: 248 4014
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4262
SmaI CCC'GGG
Cuts at: 1284 1284
Size: 4262
SmlI C'TyrA_G
Cuts at: 1417 2332 2824 3086 1417
Size: 915 492 262 2593
Fragments arranged by size:
2593 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4262
SphI G_CATG'C
Cuts at: 1315 1815 1315
Size: 500 3762
StyI C'CwwG_G
Cuts at: 107 797 1396 1750 107
Size: 690 599 354 2619
Fragments arranged by size:
2619 690 599 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 802 977 1131 1845 1978 2437 2646 802
Size: 175 154 714 133 459 209 2418
Fragments arranged by size:
2418 714 459 209 175 154 133
TatI w'GTAC_w
Cuts at: 3777 3777
Size: 4262
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3779 3811 3779
Size: 32 4230
Tth111I GACn'n_nGTC
Cuts at: 2059 2059
Size: 4262
XbaI T'CTAG_A
Cuts at: 1293 1293
Size: 4262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1757 1757
Size: 4262
XmaI C'CCGG_G
Cuts at: 1282 1282
Size: 4262
XmnI GAAnn'nnTTC
Cuts at: 535 535
Size: 4262
ZraI GAC'GTC
Cuts at: 996 996
Size: 4262
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BaeI BcgI BfrBI
BglI BmgBI BmtI BpmI BsaXI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI