Restriction map for
pF1KE0425
(Circular) (Six-base) MAPSORT of: pF1KE0425.seq Check: 9753 from: 1 to: 4232
pF1KE0425 4232 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1220 1220
Size: 4232
AccI GT'mk_AC
Cuts at: 1270 1270
Size: 4232
Acc65I G'GTAC_C
Cuts at: 741 1248 741
Size: 507 3725
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 2057 2489 3236 3504 605
Size: 1452 432 747 268 1333
Fragments arranged by size:
1452 1333 747 432 268
AflIII A'CryG_T
Cuts at: 1584 2688 3665 1584
Size: 1104 977 2151
Fragments arranged by size:
2151 1104 977
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AleI CACnn'nnGTG
Cuts at: 823 823
Size: 4232
AlwNI CAG_nnn'CTG
Cuts at: 279 378 1537 3104 279
Size: 99 1159 1567 1407
Fragments arranged by size:
1567 1407 1159 99
ApaLI G'TGCA_C
Cuts at: 3002 3002
Size: 4232
ApoI r'AATT_y
Cuts at: 681 1236 3585 3841 681
Size: 555 2349 256 1072
Fragments arranged by size:
2349 1072 555 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 872 1252 872
Size: 380 3852
BamHI G'GATC_C
Cuts at: 1257 1257
Size: 4232
BanI G'GyrC_C
Cuts at: 478 661 741 1248 1662 1910 1945 478
Size: 183 80 507 414 248 35 2765
Fragments arranged by size:
2765 507 414 248 183 80 35
BanII G_rGCy'C
Cuts at: 244 455 923 1246 2276 244
Size: 211 468 323 1030 2200
Fragments arranged by size:
2200 1030 468 323 211
BbeI G_GCGC'C
Cuts at: 665 1666 1914 665
Size: 1001 248 2983
Fragments arranged by size:
2983 1001 248
BbsI GAAGACnn'nnnn_
Cuts at: 593 593
Size: 4232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 216 250 1186 1220 216
Size: 34 936 34 3228
Fragments arranged by size:
3228 936 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 1046 1752 1046
Size: 706 3526
BglI GCCn_nnn'nGGC
Cuts at: 710 1141 710
Size: 431 3801
BglII A'GATC_T
Cuts at: 635 1747 3784 635
Size: 1112 2037 1083
Fragments arranged by size:
2037 1112 1083
BlpI GC'TnA_GC
Cuts at: 1344 1344
Size: 4232
Bme1580I G_kGCm'C
Cuts at: 1857 1950 3006 1857
Size: 93 1056 3083
Fragments arranged by size:
3083 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 895 1008 1444 1525 1860 4004 895
Size: 113 436 81 335 2144 1123
Fragments arranged by size:
2144 1123 436 335 113 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 885 2599 885
Size: 1714 2518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 2287 2779 3077 1408
Size: 879 492 298 2563
Fragments arranged by size:
2563 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 131 3936 131
Size: 3805 427
BsaAI yAC'GTr
Cuts at: 1585 1585
Size: 4232
BsaBI GATnn'nnATC
Cuts at: 6 1045 1746 3783 6
Size: 1039 701 2037 455
Fragments arranged by size:
2037 1039 701 455
BsaHI Gr'CG_yC
Cuts at: 563 662 1663 1911 2613 4109 563
Size: 99 1001 248 702 1496 686
Fragments arranged by size:
1496 1001 702 686 248 99
BsaWI w'CCGG_w
Cuts at: 1421 1942 2620 2894 3041 3452 1421
Size: 521 678 274 147 411 2201
Fragments arranged by size:
2201 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 384 511 1657 2415 2992 3488 384
Size: 127 1146 758 577 496 1128
Fragments arranged by size:
1146 1128 758 577 496 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 1216
Size: 4232
BsiEI CG_ry'CG
Cuts at: 104 1774 1820 3028 104
Size: 1670 46 1208 1308
Fragments arranged by size:
1670 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 455 1246 2024 2214 3006 455
Size: 791 778 190 792 1681
Fragments arranged by size:
1681 792 791 778 190
BsmI GAATG_Cn'
Cuts at: 189 189
Size: 4232
Bsp1286I G_dGCh'C
Cuts at: 244 455 923 1246 1857 1950 2024 2214 2276
Size: 211 468 323 611 93 74 190 62
Cuts at: 2276 3006 244
Size: 730 1470
Fragments arranged by size:
1470 730 611 468 323 211 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 3408 3408
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1220 1282 1496 1798 2179 1220
Size: 62 214 302 381 3273
Fragments arranged by size:
3273 381 302 214 62
BsrBI CCG'CTC
Cuts at: 50 2524 2578 4062 4227 50
Size: 2474 54 1484 165 55
Fragments arranged by size:
2474 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2144 2144
Size: 4232
BsrFI r'CCGG_y
Cuts at: 2230 2411 2620 2230
Size: 181 209 3842
Fragments arranged by size:
3842 209 181
BssSI C'ACGA_G
Cuts at: 2503 2861 2503
Size: 358 3874
BstAPI GCAn_nnn'nTGC
Cuts at: 1609 1609
Size: 4232
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstEII G'GTnAC_C
Cuts at: 355 355
Size: 4232
BstYI r'GATC_y
Cuts at: 1 375 635 1257 1724 1747 2082 2328 3340
Size: 374 260 622 467 23 335 246 1012
Cuts at: 3340 3426 3438 3784 1
Size: 86 12 346 449
Fragments arranged by size:
1012 622 467 449 374 346 335 260
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 840 1145 1169 107
Size: 733 305 24 3170
Fragments arranged by size:
3170 733 305 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2182 2363 2526 2182
Size: 181 163 3888
Fragments arranged by size:
3888 181 163
BtsI GCAGTG_nn'
Cuts at: 546 1475 546
Size: 929 3303
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DraIII CAC_nnn'GTG
Cuts at: 267 889 267
Size: 622 3610
DrdI GACnn_nn'nnGTC
Cuts at: 942 1938 2796 942
Size: 996 858 2378
Fragments arranged by size:
2378 996 858
EaeI y'GGCC_r
Cuts at: 1817 1991 2382 2409 3754 1817
Size: 174 391 27 1345 2295
Fragments arranged by size:
2295 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 691 2440 2750 2896 4042 691
Size: 1749 310 146 1146 881
Fragments arranged by size:
1749 1146 881 310 146
EcoICRI GAG'CTC
Cuts at: 453 1244 453
Size: 791 3441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 2057 2489 3236 3504 605
Size: 1452 432 747 268 1333
Fragments arranged by size:
1452 1333 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 754 754
Size: 4232
EcoO109I rG'GnC_Cy
Cuts at: 729 975 1371 729
Size: 246 396 3590
Fragments arranged by size:
3590 396 246
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
EcoRV GAT'ATC
Cuts at: 312 570 312
Size: 258 3974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 471 702 2251 471
Size: 231 1549 2452
Fragments arranged by size:
2452 1549 231
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 665 1666 1914 2936 3915 665
Size: 1001 248 1022 979 982
Fragments arranged by size:
1022 1001 982 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 128 160 281 313 2074 2104 2106 2136 128
Size: 32 121 32 1761 30 2 30 2224
Fragments arranged by size:
2224 1761 121 32 32 30 30 2
HincII GTy'rAC
Cuts at: 204 1271 204
Size: 1067 3165
HpaI GTT'AAC
Cuts at: 204 204
Size: 4232
KasI G'GCGC_C
Cuts at: 661 1662 1910 661
Size: 1001 248 2983
Fragments arranged by size:
2983 1001 248
KpnI G_GTAC'C
Cuts at: 745 1252 745
Size: 507 3725
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3087 2903
Size: 184 4048
MscI TGG'CCA
Cuts at: 1993 1993
Size: 4232
MslI CAynn'nnrTG
Cuts at: 823 1084 2221 2348 823
Size: 261 1137 127 2707
Fragments arranged by size:
2707 1137 261 127
MspA1I CmG'CkG
Cuts at: 1065 1124 1343 1657 2017 3030 3275 1065
Size: 59 219 314 360 1013 245 2022
Fragments arranged by size:
2022 1013 360 314 245 219 59
NaeI GCC'GGC
Cuts at: 2413 2413
Size: 4232
NarI GG'CG_CC
Cuts at: 662 1663 1911 662
Size: 1001 248 2983
Fragments arranged by size:
2983 1001 248
NcoI C'CATG_G
Cuts at: 107 840 1145 1169 107
Size: 733 305 24 3170
Fragments arranged by size:
3170 733 305 24
NgoMIV G'CCGG_C
Cuts at: 2411 2411
Size: 4232
NspI r_CATG'y
Cuts at: 1285 1785 2692 1285
Size: 500 907 2825
Fragments arranged by size:
2825 907 500
PciI A'CATG_T
Cuts at: 2688 2688
Size: 4232
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4200
PpuMI rG'GwC_Cy
Cuts at: 729 729
Size: 4232
PstI C_TGCA'G
Cuts at: 367 1070 1279 1964 367
Size: 703 209 685 2635
Fragments arranged by size:
2635 703 685 209
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 1065 1124 1657 2017 1065
Size: 59 533 360 3280
Fragments arranged by size:
3280 533 360 59
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 455 1246 455
Size: 791 3441
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SanDI GG'GwC_CC
Cuts at: 729 729
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 3749 3749
Size: 4232
SfcI C'TryA_G
Cuts at: 33 363 1066 1119 1275 1960 2953 3144 33
Size: 330 703 53 156 685 993 191 1121
Fragments arranged by size:
1121 993 703 685 330 191 156 53
SfoI GGC'GCC
Cuts at: 663 1664 1912 663
Size: 1001 248 2983
Fragments arranged by size:
2983 1001 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 1254 1254
Size: 4232
SmlI C'TyrA_G
Cuts at: 1387 2302 2794 3056 1387
Size: 915 492 262 2563
Fragments arranged by size:
2563 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1161 67
Size: 1094 3138
SphI G_CATG'C
Cuts at: 1285 1785 1285
Size: 500 3732
StuI AGG'CCT
Cuts at: 962 962
Size: 4232
StyI C'CwwG_G
Cuts at: 107 724 840 1145 1169 1366 1720 107
Size: 617 116 305 24 197 354 2619
Fragments arranged by size:
2619 617 354 305 197 116 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 343 877 990 1098 1815 1948 2407 2616 343
Size: 534 113 108 717 133 459 209 1959
Fragments arranged by size:
1959 717 534 459 209 133 113 108
TatI w'GTAC_w
Cuts at: 3747 3747
Size: 4232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4200
Tth111I GACn'n_nGTC
Cuts at: 142 2029 142
Size: 1887 2345
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1143 1727 1143
Size: 584 3648
XmaI C'CCGG_G
Cuts at: 1252 1252
Size: 4232
XmnI GAAnn'nnTTC
Cuts at: 590 682 590
Size: 92 4140
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BpmI Bpu10I
BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI FseI FspAI HindIII MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI