Restriction map for
pF1KE0424
(Circular) (Six-base) MAPSORT of: pF1KE0424.seq Check: 2511 from: 1 to: 4523
pF1KE0424 4523 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 515 1561 515
Size: 1046 3477
Acc65I G'GTAC_C
Cuts at: 1060 1539 1060
Size: 479 4044
AclI AA'CG_TT
Cuts at: 4363 4363
Size: 4523
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1282 2348 2780 3527 3795 628
Size: 654 1066 432 747 268 1356
Fragments arranged by size:
1356 1066 747 654 432 268
AflIII A'CryG_T
Cuts at: 1875 2979 3956 1875
Size: 1104 977 2442
Fragments arranged by size:
2442 1104 977
AgeI A'CCGG_T
Cuts at: 2911 2911
Size: 4523
AlwNI CAG_nnn'CTG
Cuts at: 726 1111 1358 1828 3395 726
Size: 385 247 470 1567 1854
Fragments arranged by size:
1854 1567 470 385 247
ApaI G_GGCC'C
Cuts at: 921 1199 1492 921
Size: 278 293 3952
Fragments arranged by size:
3952 293 278
ApaLI G'TGCA_C
Cuts at: 3293 3293
Size: 4523
ApoI r'AATT_y
Cuts at: 1527 3876 4132 1527
Size: 2349 256 1918
Fragments arranged by size:
2349 1918 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4523
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4523
AvaI C'yCGr_G
Cuts at: 131 562 1543 131
Size: 431 981 3111
Fragments arranged by size:
3111 981 431
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1052 1085 1052
Size: 33 4490
BamHI G'GATC_C
Cuts at: 905 1548 905
Size: 643 3880
BanI G'GyrC_C
Cuts at: 678 1060 1539 1953 2201 2236 678
Size: 382 479 414 248 35 2965
Fragments arranged by size:
2965 479 414 382 248 35
BanII G_rGCy'C
Cuts at: 282 921 1199 1492 1537 2567 282
Size: 639 278 293 45 1030 2238
Fragments arranged by size:
2238 1030 639 293 278 45
BbeI G_GCGC'C
Cuts at: 1957 2205 1957
Size: 248 4275
BbvCI CC'TCA_GC
Cuts at: 1450 1450
Size: 4523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 505 539 505
Size: 34 4489
BciVI GTATCCnnnnn_n'
Cuts at: 2421 3182 4523 2421
Size: 761 1341 2421
Fragments arranged by size:
2421 1341 761
BclI T'GATC_A
Cuts at: 2043 2043
Size: 4523
BglI GCCn_nnn'nGGC
Cuts at: 112 814 112
Size: 702 3821
BglII A'GATC_T
Cuts at: 2038 4075 2038
Size: 2037 2486
BlpI GC'TnA_GC
Cuts at: 397 1635 397
Size: 1238 3285
Bme1580I G_kGCm'C
Cuts at: 921 1199 1492 2148 2241 3297 921
Size: 278 293 656 93 1056 2147
Fragments arranged by size:
2147 1056 656 293 278 93
BmrI ACTGGGnnnn_n'
Cuts at: 1099 1735 1816 2151 4295 1099
Size: 636 81 335 2144 1327
Fragments arranged by size:
2144 1327 636 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1359 2890 1359
Size: 1531 2992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 373
Size: 4523
Bpu10I CC'TnA_GC
Cuts at: 727 1237 1450 727
Size: 510 213 3800
Fragments arranged by size:
3800 510 213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1441 1699 2578 3070 3368 721
Size: 720 258 879 492 298 1876
Fragments arranged by size:
1876 879 720 492 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 4227 4227
Size: 4523
BsaAI yAC'GTr
Cuts at: 1876 1876
Size: 4523
BsaBI GATnn'nnATC
Cuts at: 6 2037 4074 6
Size: 2031 2037 455
Fragments arranged by size:
2037 2031 455
BsaHI Gr'CG_yC
Cuts at: 1954 2202 2904 4400 1954
Size: 248 702 1496 2077
Fragments arranged by size:
2077 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1712 2233 2911 3185 3332 3743 1712
Size: 521 678 274 147 411 2492
Fragments arranged by size:
2492 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 483 1246 483
Size: 763 3760
BseYI C'CCAG_C
Cuts at: 357 1198 1948 2706 3283 3779 357
Size: 841 750 758 577 496 1101
Fragments arranged by size:
1101 841 758 750 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1423 1003
Size: 420 4103
BsiEI CG_ry'CG
Cuts at: 104 290 2065 2111 3319 104
Size: 186 1775 46 1208 1308
Fragments arranged by size:
1775 1308 1208 186 46
BsiHKAI G_wGCw'C
Cuts at: 733 1537 2315 2505 3297 733
Size: 804 778 190 792 1959
Fragments arranged by size:
1959 804 792 778 190
BsmI GAATG_Cn'
Cuts at: 477 477
Size: 4523
Bsp1286I G_dGCh'C
Cuts at: 282 733 921 1199 1492 1537 2148 2241 2315
Size: 451 188 278 293 45 611 93 74
Cuts at: 2315 2505 2567 3297 282
Size: 190 62 730 1508
Fragments arranged by size:
1508 730 611 451 293 278 190 188
93 74 62 45
BspEI T'CCGG_A
Cuts at: 3743 3743
Size: 4523
BspHI T'CATG_A
Cuts at: 297 3699 297
Size: 3402 1121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 228 557 711 1573 1787 2089 2470 228
Size: 329 154 862 214 302 381 2281
Fragments arranged by size:
2281 862 381 329 302 214 154
BsrBI CCG'CTC
Cuts at: 50 898 2815 2869 4353 4518 50
Size: 848 1917 54 1484 165 55
Fragments arranged by size:
1917 1484 848 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1161 2435 1161
Size: 1274 3249
BsrFI r'CCGG_y
Cuts at: 186 2521 2702 2911 186
Size: 2335 181 209 1798
Fragments arranged by size:
2335 1798 209 181
BssSI C'ACGA_G
Cuts at: 1258 2794 3152 1258
Size: 1536 358 2629
Fragments arranged by size:
2629 1536 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1293 1900 1293
Size: 607 3916
BstBI TT'CG_AA
Cuts at: 2884 2884
Size: 4523
BstXI CCAn_nnnn'nTGG
Cuts at: 1156 1156
Size: 4523
BstYI r'GATC_y
Cuts at: 1 342 905 1310 1548 2015 2038 2373 2619
Size: 341 563 405 238 467 23 335 246
Cuts at: 2619 3631 3717 3729 4075 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 563 467 449 405 346 341 335
246 238 86 23 12
BtgI C'CryG_G
Cuts at: 119 148 178 809 1130 1463 119
Size: 29 30 631 321 333 3179
Fragments arranged by size:
3179 631 333 321 30 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2473 2654 2817 2473
Size: 181 163 4179
Fragments arranged by size:
4179 181 163
BtsI GCAGTG_nn'
Cuts at: 1215 1766 1215
Size: 551 3972
DraI TTT'AAA
Cuts at: 1522 1522
Size: 4523
DrdI GACnn_nn'nnGTC
Cuts at: 2229 3087 2229
Size: 858 3665
EaeI y'GGCC_r
Cuts at: 116 287 590 815 1088 2108 2282 2673 2700
Size: 171 303 225 273 1020 174 391 27
Cuts at: 2700 4045 116
Size: 1345 594
Fragments arranged by size:
1345 1020 594 391 303 273 225 174
171 27
EagI C'GGCC_G
Cuts at: 287 2108 287
Size: 1821 2702
EarI CTCTTCn'nnn_
Cuts at: 1423 2546 2756 1423
Size: 1123 210 3190
Fragments arranged by size:
3190 1123 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 244 769 2731 3041 3187 4333 244
Size: 525 1962 310 146 1146 434
Fragments arranged by size:
1962 1146 525 434 310 146
EcoICRI GAG'CTC
Cuts at: 1535 1535
Size: 4523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 628 1282 2348 2780 3527 3795 373
Size: 255 654 1066 432 747 268 1101
Fragments arranged by size:
1101 1066 747 654 432 268 255
EcoNI CCTnn'n_nnAGG
Cuts at: 1026 1026
Size: 4523
EcoO109I rG'GnC_Cy
Cuts at: 240 361 723 957 1195 1488 1489 1662 240
Size: 121 362 234 238 293 1 173 3101
Fragments arranged by size:
3101 362 293 238 234 173 121 1
EcoRI G'AATT_C
Cuts at: 1527 1527
Size: 4523
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4523
FspI TGC'GCA
Cuts at: 2304 2304
Size: 4523
HaeII r_GCGC'y
Cuts at: 166 455 1140 1957 2205 3227 4206 166
Size: 289 685 817 248 1022 979 483
Fragments arranged by size:
1022 979 817 685 483 289 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 858 890 1452 1484 2365 2395 2397 2427 858
Size: 32 562 32 881 30 2 30 2954
Fragments arranged by size:
2954 881 562 32 32 30 30 2
HincII GTy'rAC
Cuts at: 516 1562 516
Size: 1046 3477
KasI G'GCGC_C
Cuts at: 1953 2201 1953
Size: 248 4275
KpnI G_GTAC'C
Cuts at: 1064 1543 1064
Size: 479 4044
MluI A'CGCG_T
Cuts at: 3956 3956
Size: 4523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3194 3378 3194
Size: 184 4339
MscI TGG'CCA
Cuts at: 592 1090 2284 592
Size: 498 1194 2831
Fragments arranged by size:
2831 1194 498
MslI CAynn'nnrTG
Cuts at: 1154 1387 2512 2639 1154
Size: 233 1125 127 3038
Fragments arranged by size:
3038 1125 233 127
MspA1I CmG'CkG
Cuts at: 150 226 246 763 775 837 1634 1948 2308
Size: 76 20 517 12 62 797 314 360
Cuts at: 2308 3321 3566 150
Size: 1013 245 1107
Fragments arranged by size:
1107 1013 797 517 360 314 245 76
62 20 12
NaeI GCC'GGC
Cuts at: 2704 2704
Size: 4523
NarI GG'CG_CC
Cuts at: 1954 2202 1954
Size: 248 4275
NcoI C'CATG_G
Cuts at: 119 1130 1463 119
Size: 1011 333 3179
Fragments arranged by size:
3179 1011 333
NdeI CA'TA_TG
Cuts at: 420 420
Size: 4523
NgoMIV G'CCGG_C
Cuts at: 2702 2702
Size: 4523
NspI r_CATG'y
Cuts at: 177 583 1576 2076 2983 177
Size: 406 993 500 907 1717
Fragments arranged by size:
1717 993 907 500 406
PasI CC'CwG_GG
Cuts at: 683 683
Size: 4523
PciI A'CATG_T
Cuts at: 2979 2979
Size: 4523
PflMI CCAn_nnn'nTGG
Cuts at: 1515 1515
Size: 4523
PmeI GTTT'AAAC
Cuts at: 1522 1522
Size: 4523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 3662
Size: 32 4491
PpuMI rG'GwC_Cy
Cuts at: 240 361 723 957 240
Size: 121 362 234 3806
Fragments arranged by size:
3806 362 234 121
PspOMI G'GGCC_C
Cuts at: 917 1195 1488 917
Size: 278 293 3952
Fragments arranged by size:
3952 293 278
PspXI vC'TCGA_Gb
Cuts at: 131 131
Size: 4523
PstI C_TGCA'G
Cuts at: 389 554 1570 2255 389
Size: 165 1016 685 2657
Fragments arranged by size:
2657 1016 685 165
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4523
PvuII CAG'CTG
Cuts at: 837 1948 2308 837
Size: 1111 360 3052
Fragments arranged by size:
3052 1111 360
RsrII CG'GwC_CG
Cuts at: 777 2718 777
Size: 1941 2582
SacI G_AGCT'C
Cuts at: 1537 1537
Size: 4523
SacII CC_GC'GG
Cuts at: 151 151
Size: 4523
SalI G'TCGA_C
Cuts at: 514 1560 514
Size: 1046 3477
SanDI GG'GwC_CC
Cuts at: 361 361
Size: 4523
SapI GCTCTTCn'nnn_
Cuts at: 1423 2546 2756 1423
Size: 1123 210 3190
Fragments arranged by size:
3190 1123 210
SbfI CC_TGCA'GG
Cuts at: 389 554 1570 389
Size: 165 1016 3342
Fragments arranged by size:
3342 1016 165
ScaI AGT'ACT
Cuts at: 4040 4040
Size: 4523
SfcI C'TryA_G
Cuts at: 33 385 550 1566 2251 3244 3435 33
Size: 352 165 1016 685 993 191 1121
Fragments arranged by size:
1121 1016 993 685 352 191 165
SfoI GGC'GCC
Cuts at: 1955 2203 1955
Size: 248 4275
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4523
SmaI CCC'GGG
Cuts at: 1545 1545
Size: 4523
SmlI C'TyrA_G
Cuts at: 131 736 1456 1678 2593 3085 3347 131
Size: 605 720 222 915 492 262 1307
Fragments arranged by size:
1307 915 720 605 492 262 222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4523
SphI G_CATG'C
Cuts at: 177 1576 2076 177
Size: 1399 500 2624
Fragments arranged by size:
2624 1399 500
StyI C'CwwG_G
Cuts at: 119 1130 1463 1657 2011 119
Size: 1011 333 194 354 2631
Fragments arranged by size:
2631 1011 354 333 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 696 2106 2239 2698 2907 696
Size: 1410 133 459 209 2312
Fragments arranged by size:
2312 1410 459 209 133
TatI w'GTAC_w
Cuts at: 4038 4038
Size: 4523
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 851 883 4040 4072 851
Size: 32 3157 32 1302
Fragments arranged by size:
3157 1302 32 32
Tth111I GACn'n_nGTC
Cuts at: 2320 2320
Size: 4523
XbaI T'CTAG_A
Cuts at: 1554 1554
Size: 4523
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2018 2018
Size: 4523
XhoI C'TCGA_G
Cuts at: 131 131
Size: 4523
XmaI C'CCGG_G
Cuts at: 1543 1543
Size: 4523
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbsI BfrBI BmgBI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PsrI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI