Restriction map for
pF1KE0422
(Circular) (Six-base) MAPSORT of: pF1KE0422.seq Check: 8460 from: 1 to: 4292
pF1KE0422 4292 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AatII G_ACGT'C
Cuts at: 1244 1244
Size: 4292
AccI GT'mk_AC
Cuts at: 1330 1330
Size: 4292
Acc65I G'GTAC_C
Cuts at: 1308 1308
Size: 4292
AclI AA'CG_TT
Cuts at: 4132 4132
Size: 4292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 321 469 694 1247 2117 2549 3296 3564
Size: 185 148 225 553 870 432 747 268
Cuts at: 3564 136
Size: 864
Fragments arranged by size:
870 864 747 553 432 268 225 185
148
AflIII A'CryG_T
Cuts at: 1644 2748 3725 1644
Size: 1104 977 2211
Fragments arranged by size:
2211 1104 977
AgeI A'CCGG_T
Cuts at: 2680 2680
Size: 4292
AleI CACnn'nnGTG
Cuts at: 347 347
Size: 4292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1154 1154
Size: 4292
AlwNI CAG_nnn'CTG
Cuts at: 1597 3164 1597
Size: 1567 2725
ApaI G_GGCC'C
Cuts at: 979 979
Size: 4292
ApaLI G'TGCA_C
Cuts at: 373 763 3062 373
Size: 390 2299 1603
Fragments arranged by size:
2299 1603 390
ApoI r'AATT_y
Cuts at: 1296 3645 3901 1296
Size: 2349 256 1687
Fragments arranged by size:
2349 1687 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4292
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
AvaI C'yCGr_G
Cuts at: 157 213 1312 157
Size: 56 1099 3137
Fragments arranged by size:
3137 1099 56
BamHI G'GATC_C
Cuts at: 1317 1317
Size: 4292
BanI G'GyrC_C
Cuts at: 196 1308 1722 1970 2005 196
Size: 1112 414 248 35 2483
Fragments arranged by size:
2483 1112 414 248 35
BanII G_rGCy'C
Cuts at: 314 979 1306 2336 314
Size: 665 327 1030 2270
Fragments arranged by size:
2270 1030 665 327
BbeI G_GCGC'C
Cuts at: 200 1726 1974 200
Size: 1526 248 2518
Fragments arranged by size:
2518 1526 248
BbsI GAAGACnn'nnnn_
Cuts at: 327 327
Size: 4292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 135 169 478 512 1038 1072 135
Size: 34 309 34 526 34 3355
Fragments arranged by size:
3355 526 309 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2190 2951 4292 2190
Size: 761 1341 2190
Fragments arranged by size:
2190 1341 761
BclI T'GATC_A
Cuts at: 1812 1812
Size: 4292
BglI GCCn_nnn'nGGC
Cuts at: 148 421 563 1282 148
Size: 273 142 719 3158
Fragments arranged by size:
3158 719 273 142
BglII A'GATC_T
Cuts at: 1807 3844 1807
Size: 2037 2255
BlpI GC'TnA_GC
Cuts at: 1404 1404
Size: 4292
Bme1580I G_kGCm'C
Cuts at: 377 767 979 1219 1917 2010 3066 377
Size: 390 212 240 698 93 1056 1603
Fragments arranged by size:
1603 1056 698 390 240 212 93
BmrI ACTGGGnnnn_n'
Cuts at: 796 1504 1585 1920 4064 796
Size: 708 81 335 2144 1024
Fragments arranged by size:
2144 1024 708 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 228 2659 228
Size: 2431 1861
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 790 658
Size: 132 4160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1108 1468 2347 2839 3137 1108
Size: 360 879 492 298 2263
Fragments arranged by size:
2263 879 492 360 298
BsaI GGTCTCn'nnnn_
Cuts at: 3996 3996
Size: 4292
BsaAI yAC'GTr
Cuts at: 378 1645 378
Size: 1267 3025
BsaBI GATnn'nnATC
Cuts at: 6 1806 3843 6
Size: 1800 2037 455
Fragments arranged by size:
2037 1800 455
BsaHI Gr'CG_yC
Cuts at: 197 1241 1723 1971 2673 4169 197
Size: 1044 482 248 702 1496 320
Fragments arranged by size:
1496 1044 702 482 320 248
BsaWI w'CCGG_w
Cuts at: 1197 1481 2002 2680 2954 3101 3512 1197
Size: 284 521 678 274 147 411 1977
Fragments arranged by size:
1977 678 521 411 284 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 228 258 228
Size: 30 4262
BseYI C'CCAG_C
Cuts at: 899 991 1717 2475 3052 3548 899
Size: 92 726 758 577 496 1643
Fragments arranged by size:
1643 758 726 577 496 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1114
Size: 4292
BsiEI CG_ry'CG
Cuts at: 104 128 761 1272 1834 1880 3088 104
Size: 24 633 511 562 46 1208 1308
Fragments arranged by size:
1308 1208 633 562 511 46 24
BsiHKAI G_wGCw'C
Cuts at: 158 314 377 767 1306 2084 2274 3066 158
Size: 156 63 390 539 778 190 792 1384
Fragments arranged by size:
1384 792 778 539 390 190 156 63
Bsp1286I G_dGCh'C
Cuts at: 158 314 377 767 979 1219 1306 1917 2010
Size: 156 63 390 212 240 87 611 93
Cuts at: 2010 2084 2274 2336 3066 158
Size: 74 190 62 730 1384
Fragments arranged by size:
1384 730 611 390 240 212 190 156
93 87 74 63 62
BspEI T'CCGG_A
Cuts at: 1197 3512 1197
Size: 2315 1977
BspHI T'CATG_A
Cuts at: 779 3468 779
Size: 2689 1603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 485 1342 1556 1858 2239 485
Size: 857 214 302 381 2538
Fragments arranged by size:
2538 857 381 302 214
BsrBI CCG'CTC
Cuts at: 50 740 2584 2638 4122 4287 50
Size: 690 1844 54 1484 165 55
Fragments arranged by size:
1844 1484 690 165 55 54
BsrDI GCAATG_nn'
Cuts at: 417 704 2204 417
Size: 287 1500 2505
Fragments arranged by size:
2505 1500 287
BsrFI r'CCGG_y
Cuts at: 133 1045 2290 2471 2680 133
Size: 912 1245 181 209 1745
Fragments arranged by size:
1745 1245 912 209 181
BssSI C'ACGA_G
Cuts at: 202 2563 2921 202
Size: 2361 358 1573
Fragments arranged by size:
2361 1573 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1669 1669
Size: 4292
BstBI TT'CG_AA
Cuts at: 2653 2653
Size: 4292
BstEII G'GTnAC_C
Cuts at: 276 570 276
Size: 294 3998
BstXI CCAn_nnnn'nTGG
Cuts at: 885 1095 885
Size: 210 4082
BstYI r'GATC_y
Cuts at: 1 1317 1784 1807 2142 2388 3400 3486 3498
Size: 1316 467 23 335 246 1012 86 12
Cuts at: 3498 3844 1
Size: 346 449
Fragments arranged by size:
1316 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1082 1172 1277 107
Size: 975 90 105 3122
Fragments arranged by size:
3122 975 105 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 806 1056 2242 2423 2586 806
Size: 250 1186 181 163 2512
Fragments arranged by size:
2512 1186 250 181 163
BtsI GCAGTG_nn'
Cuts at: 1535 1535
Size: 4292
DraI TTT'AAA
Cuts at: 1291 1291
Size: 4292
DraIII CAC_nnn'GTG
Cuts at: 276 276
Size: 4292
DrdI GACnn_nn'nnGTC
Cuts at: 1998 2856 1998
Size: 858 3434
EaeI y'GGCC_r
Cuts at: 422 758 1005 1085 1097 1269 1877 2051 2442
Size: 336 247 80 12 172 608 174 391
Cuts at: 2442 2469 3814 422
Size: 27 1345 900
Fragments arranged by size:
1345 900 608 391 336 247 174 172
80 27 12
EagI C'GGCC_G
Cuts at: 758 1269 1877 758
Size: 511 608 3173
Fragments arranged by size:
3173 608 511
EarI CTCTTCn'nnn_
Cuts at: 362 392 2315 2525 362
Size: 30 1923 210 2129
Fragments arranged by size:
2129 1923 210 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 418 1237 2500 2810 2956 4102 418
Size: 819 1263 310 146 1146 608
Fragments arranged by size:
1263 1146 819 608 310 146
EcoICRI GAG'CTC
Cuts at: 312 1304 312
Size: 992 3300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 321 469 658 694 790 1247 2117 2549
Size: 185 148 189 36 96 457 870 432
Cuts at: 2549 3296 3564 136
Size: 747 268 864
Fragments arranged by size:
870 864 747 457 432 268 189 185
148 96 36
EcoO109I rG'GnC_Cy
Cuts at: 207 975 1431 207
Size: 768 456 3068
Fragments arranged by size:
3068 768 456
EcoRI G'AATT_C
Cuts at: 1296 1296
Size: 4292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 351 2311 351
Size: 1960 2332
FspI TGC'GCA
Cuts at: 703 2073 703
Size: 1370 2922
HaeII r_GCGC'y
Cuts at: 115 143 200 1726 1974 2996 3975 115
Size: 28 57 1526 248 1022 979 432
Fragments arranged by size:
1526 1022 979 432 248 57 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 912 944 2134 2164 2166 2196 912
Size: 32 1190 30 2 30 3008
Fragments arranged by size:
3008 1190 32 30 30 2
HincII GTy'rAC
Cuts at: 948 1331 948
Size: 383 3909
KasI G'GCGC_C
Cuts at: 196 1722 1970 196
Size: 1526 248 2518
Fragments arranged by size:
2518 1526 248
KpnI G_GTAC'C
Cuts at: 1312 1312
Size: 4292
MluI A'CGCG_T
Cuts at: 3725 3725
Size: 4292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2963 3147 2963
Size: 184 4108
MscI TGG'CCA
Cuts at: 424 1007 1099 2053 424
Size: 583 92 954 2663
Fragments arranged by size:
2663 954 583 92
MslI CAynn'nnrTG
Cuts at: 347 647 862 877 919 1136 2281 2408 347
Size: 300 215 15 42 217 1145 127 2231
Fragments arranged by size:
2231 1145 300 217 215 127 42 15
MspA1I CmG'CkG
Cuts at: 885 1103 1403 1717 2077 3090 3335 885
Size: 218 300 314 360 1013 245 1842
Fragments arranged by size:
1842 1013 360 314 300 245 218
NaeI GCC'GGC
Cuts at: 135 2473 135
Size: 2338 1954
NarI GG'CG_CC
Cuts at: 197 1723 1971 197
Size: 1526 248 2518
Fragments arranged by size:
2518 1526 248
NcoI C'CATG_G
Cuts at: 107 1172 107
Size: 1065 3227
NgoMIV G'CCGG_C
Cuts at: 133 2471 133
Size: 2338 1954
NspI r_CATG'y
Cuts at: 325 350 1345 1845 2752 325
Size: 25 995 500 907 1865
Fragments arranged by size:
1865 995 907 500 25
PasI CC'CwG_GG
Cuts at: 182 289 182
Size: 107 4185
PciI A'CATG_T
Cuts at: 2748 2748
Size: 4292
PflMI CCAn_nnn'nTGG
Cuts at: 295 295
Size: 4292
PfoI T'CCnGG_A
Cuts at: 605 1235 605
Size: 630 3662
PmeI GTTT'AAAC
Cuts at: 1291 1291
Size: 4292
PmlI CAC'GTG
Cuts at: 378 378
Size: 4292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3431 3463 3431
Size: 32 4260
PpuMI rG'GwC_Cy
Cuts at: 207 207
Size: 4292
PspOMI G'GGCC_C
Cuts at: 975 975
Size: 4292
PspXI vC'TCGA_Gb
Cuts at: 213 213
Size: 4292
PstI C_TGCA'G
Cuts at: 817 1339 2024 817
Size: 522 685 3085
Fragments arranged by size:
3085 685 522
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4292
PvuII CAG'CTG
Cuts at: 885 1103 1717 2077 885
Size: 218 614 360 3100
Fragments arranged by size:
3100 614 360 218
RsrII CG'GwC_CG
Cuts at: 2487 2487
Size: 4292
SacI G_AGCT'C
Cuts at: 314 1306 314
Size: 992 3300
SalI G'TCGA_C
Cuts at: 1329 1329
Size: 4292
SapI GCTCTTCn'nnn_
Cuts at: 2315 2525 2315
Size: 210 4082
SbfI CC_TGCA'GG
Cuts at: 1339 1339
Size: 4292
ScaI AGT'ACT
Cuts at: 3809 3809
Size: 4292
SfcI C'TryA_G
Cuts at: 33 813 1335 2020 3013 3204 33
Size: 780 522 685 993 191 1121
Fragments arranged by size:
1121 993 780 685 522 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1282 1282
Size: 4292
SfoI GGC'GCC
Cuts at: 198 1724 1972 198
Size: 1526 248 2518
Fragments arranged by size:
2518 1526 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
SmaI CCC'GGG
Cuts at: 1314 1314
Size: 4292
SmlI C'TyrA_G
Cuts at: 213 1123 1447 2362 2854 3116 213
Size: 910 324 915 492 262 1389
Fragments arranged by size:
1389 915 910 492 324 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4292
SphI G_CATG'C
Cuts at: 1345 1845 1345
Size: 500 3792
StuI AGG'CCT
Cuts at: 983 983
Size: 4292
StyI C'CwwG_G
Cuts at: 107 686 1172 1426 1780 107
Size: 579 486 254 354 2619
Fragments arranged by size:
2619 579 486 354 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1875 2008 2467 2676 1875
Size: 133 459 209 3491
Fragments arranged by size:
3491 459 209 133
TatI w'GTAC_w
Cuts at: 626 3807 626
Size: 3181 1111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 367 399 3809 3841 367
Size: 32 3410 32 818
Fragments arranged by size:
3410 818 32 32
Tth111I GACn'n_nGTC
Cuts at: 2089 2089
Size: 4292
XbaI T'CTAG_A
Cuts at: 1323 1323
Size: 4292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 882 1787 882
Size: 905 3387
XhoI C'TCGA_G
Cuts at: 213 213
Size: 4292
XmaI C'CCGG_G
Cuts at: 1312 1312
Size: 4292
ZraI GAC'GTC
Cuts at: 1242 1242
Size: 4292
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BfrBI BmgBI BmtI Bpu10I BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PsrI SacII
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XmnI