Restriction map for
pF1KE0421
(Circular) (Six-base) MAPSORT of: pF1KE0421.seq Check: 2994 from: 1 to: 3680
pF1KE0421 3680 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 718 718
Size: 3680
Acc65I G'GTAC_C
Cuts at: 696 696
Size: 3680
AclI AA'CG_TT
Cuts at: 3520 3520
Size: 3680
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 448 565 1505 1937 2684 2952 320
Size: 128 117 940 432 747 268 1048
Fragments arranged by size:
1048 940 747 432 268 128 117
AflIII A'CryG_T
Cuts at: 192 1032 2136 3113 192
Size: 840 1104 977 759
Fragments arranged by size:
1104 977 840 759
AgeI A'CCGG_T
Cuts at: 2068 2068
Size: 3680
AlwNI CAG_nnn'CTG
Cuts at: 985 2552 985
Size: 1567 2113
ApaLI G'TGCA_C
Cuts at: 393 2450 393
Size: 2057 1623
ApoI r'AATT_y
Cuts at: 255 280 684 3033 3289 255
Size: 25 404 2349 256 646
Fragments arranged by size:
2349 646 404 256 25
AseI AT'TA_AT
Cuts at: 20 20
Size: 3680
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3680
AvaI C'yCGr_G
Cuts at: 700 700
Size: 3680
BamHI G'GATC_C
Cuts at: 705 705
Size: 3680
BanI G'GyrC_C
Cuts at: 696 1110 1358 1393 696
Size: 414 248 35 2983
Fragments arranged by size:
2983 414 248 35
BanII G_rGCy'C
Cuts at: 694 1724 694
Size: 1030 2650
BbeI G_GCGC'C
Cuts at: 1114 1362 1114
Size: 248 3432
BbvCI CC'TCA_GC
Cuts at: 593 593
Size: 3680
BciVI GTATCCnnnnn_n'
Cuts at: 1578 2339 3680 1578
Size: 761 1341 1578
Fragments arranged by size:
1578 1341 761
BclI T'GATC_A
Cuts at: 1200 1200
Size: 3680
BglI GCCn_nnn'nGGC
Cuts at: 303 303
Size: 3680
BglII A'GATC_T
Cuts at: 1195 3232 1195
Size: 2037 1643
BlpI GC'TnA_GC
Cuts at: 792 792
Size: 3680
Bme1580I G_kGCm'C
Cuts at: 397 1305 1398 2454 397
Size: 908 93 1056 1623
Fragments arranged by size:
1623 1056 908 93
BmrI ACTGGGnnnn_n'
Cuts at: 491 644 892 973 1308 3452 491
Size: 153 248 81 335 2144 719
Fragments arranged by size:
2144 719 335 248 153 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 229 2047 229
Size: 1818 1862
Bpu10I CC'TnA_GC
Cuts at: 593 593
Size: 3680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 1735 2227 2525 856
Size: 879 492 298 2011
Fragments arranged by size:
2011 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3384 3384
Size: 3680
BsaAI yAC'GTr
Cuts at: 127 193 1033 127
Size: 66 840 2774
Fragments arranged by size:
2774 840 66
BsaBI GATnn'nnATC
Cuts at: 6 1194 3231 6
Size: 1188 2037 455
Fragments arranged by size:
2037 1188 455
BsaHI Gr'CG_yC
Cuts at: 1111 1359 2061 3557 1111
Size: 248 702 1496 1234
Fragments arranged by size:
1496 1234 702 248
BsaWI w'CCGG_w
Cuts at: 869 1390 2068 2342 2489 2900 869
Size: 521 678 274 147 411 1649
Fragments arranged by size:
1649 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 589 619 589
Size: 30 3650
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 610 610
Size: 3680
BseYI C'CCAG_C
Cuts at: 362 1105 1863 2440 2936 362
Size: 743 758 577 496 1106
Fragments arranged by size:
1106 758 743 577 496
BsiEI CG_ry'CG
Cuts at: 104 1222 1268 2476 104
Size: 1118 46 1208 1308
Fragments arranged by size:
1308 1208 1118 46
BsiHKAI G_wGCw'C
Cuts at: 397 694 1472 1662 2454 397
Size: 297 778 190 792 1623
Fragments arranged by size:
1623 792 778 297 190
Bsp1286I G_dGCh'C
Cuts at: 397 694 1305 1398 1472 1662 1724 2454 397
Size: 297 611 93 74 190 62 730 1623
Fragments arranged by size:
1623 730 611 297 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2900 2900
Size: 3680
BspHI T'CATG_A
Cuts at: 2856 2856
Size: 3680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 730 944 1246 1627 730
Size: 214 302 381 2783
Fragments arranged by size:
2783 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1972 2026 3510 3675 50
Size: 1922 54 1484 165 55
Fragments arranged by size:
1922 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1592 1592
Size: 3680
BsrFI r'CCGG_y
Cuts at: 1678 1859 2068 1678
Size: 181 209 3290
Fragments arranged by size:
3290 209 181
BssHII G'CGCG_C
Cuts at: 157 157
Size: 3680
BssSI C'ACGA_G
Cuts at: 1951 2309 1951
Size: 358 3322
BstAPI GCAn_nnn'nTGC
Cuts at: 1057 1057
Size: 3680
BstBI TT'CG_AA
Cuts at: 2041 2041
Size: 3680
BstXI CCAn_nnnn'nTGG
Cuts at: 418 418
Size: 3680
BstYI r'GATC_y
Cuts at: 1 183 548 705 1172 1195 1530 1776 2788
Size: 182 365 157 467 23 335 246 1012
Cuts at: 2788 2874 2886 3232 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 365 346 335 246 182
157 86 23 12
BtgI C'CryG_G
Cuts at: 107 167 298 107
Size: 60 131 3489
Fragments arranged by size:
3489 131 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1630 1811 1974 1630
Size: 181 163 3336
Fragments arranged by size:
3336 181 163
BtsI GCAGTG_nn'
Cuts at: 923 923
Size: 3680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 422 457 422
Size: 35 3645
DraI TTT'AAA
Cuts at: 679 679
Size: 3680
DrdI GACnn_nn'nnGTC
Cuts at: 1386 2244 1386
Size: 858 2822
EaeI y'GGCC_r
Cuts at: 224 1265 1439 1830 1857 3202 224
Size: 1041 174 391 27 1345 702
Fragments arranged by size:
1345 1041 702 391 174 27
EagI C'GGCC_G
Cuts at: 1265 1265
Size: 3680
EarI CTCTTCn'nnn_
Cuts at: 1703 1913 1703
Size: 210 3470
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1888 2198 2344 3490 1888
Size: 310 146 1146 2078
Fragments arranged by size:
2078 1146 310 146
EcoICRI GAG'CTC
Cuts at: 692 692
Size: 3680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 448 565 1505 1937 2684 2952 320
Size: 128 117 940 432 747 268 1048
Fragments arranged by size:
1048 940 747 432 268 128 117
EcoO109I rG'GnC_Cy
Cuts at: 819 819
Size: 3680
EcoRI G'AATT_C
Cuts at: 684 684
Size: 3680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1699 1699
Size: 3680
FspI TGC'GCA
Cuts at: 1461 1461
Size: 3680
HaeII r_GCGC'y
Cuts at: 1114 1362 2384 3363 1114
Size: 248 1022 979 1431
Fragments arranged by size:
1431 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 443 475 589 621 1522 1552 1554 1584 443
Size: 32 114 32 901 30 2 30 2539
Fragments arranged by size:
2539 901 114 32 32 30 30 2
HincII GTy'rAC
Cuts at: 498 719 498
Size: 221 3459
KasI G'GCGC_C
Cuts at: 1110 1358 1110
Size: 248 3432
KpnI G_GTAC'C
Cuts at: 700 700
Size: 3680
MluI A'CGCG_T
Cuts at: 3113 3113
Size: 3680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 399 2351 2535 399
Size: 1952 184 1544
Fragments arranged by size:
1952 1544 184
MscI TGG'CCA
Cuts at: 1441 1441
Size: 3680
MslI CAynn'nnrTG
Cuts at: 220 1669 1796 220
Size: 1449 127 2104
Fragments arranged by size:
2104 1449 127
MspA1I CmG'CkG
Cuts at: 169 366 439 543 791 1105 1465 2478 2723
Size: 197 73 104 248 314 360 1013 245
Cuts at: 2723 169
Size: 1126
Fragments arranged by size:
1126 1013 360 314 248 245 197 104
73
NaeI GCC'GGC
Cuts at: 1861 1861
Size: 3680
NarI GG'CG_CC
Cuts at: 1111 1359 1111
Size: 248 3432
NcoI C'CATG_G
Cuts at: 107 298 107
Size: 191 3489
NgoMIV G'CCGG_C
Cuts at: 1859 1859
Size: 3680
NruI TCG'CGA
Cuts at: 204 204
Size: 3680
NspI r_CATG'y
Cuts at: 733 1233 2140 733
Size: 500 907 2273
Fragments arranged by size:
2273 907 500
PciI A'CATG_T
Cuts at: 2136 2136
Size: 3680
PflMI CCAn_nnn'nTGG
Cuts at: 144 144
Size: 3680
PmeI GTTT'AAAC
Cuts at: 679 679
Size: 3680
PmlI CAC'GTG
Cuts at: 127 127
Size: 3680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2851 2819
Size: 32 3648
PstI C_TGCA'G
Cuts at: 727 1412 727
Size: 685 2995
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3680
PvuII CAG'CTG
Cuts at: 366 439 543 1105 1465 366
Size: 73 104 562 360 2581
Fragments arranged by size:
2581 562 360 104 73
RsrII CG'GwC_CG
Cuts at: 1875 1875
Size: 3680
SacI G_AGCT'C
Cuts at: 694 694
Size: 3680
SacII CC_GC'GG
Cuts at: 170 170
Size: 3680
SalI G'TCGA_C
Cuts at: 717 717
Size: 3680
SapI GCTCTTCn'nnn_
Cuts at: 1703 1913 1703
Size: 210 3470
SbfI CC_TGCA'GG
Cuts at: 727 727
Size: 3680
ScaI AGT'ACT
Cuts at: 3197 3197
Size: 3680
SfcI C'TryA_G
Cuts at: 33 723 1408 2401 2592 33
Size: 690 685 993 191 1121
Fragments arranged by size:
1121 993 690 685 191
SfoI GGC'GCC
Cuts at: 1112 1360 1112
Size: 248 3432
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3680
SmaI CCC'GGG
Cuts at: 702 702
Size: 3680
SmlI C'TyrA_G
Cuts at: 835 1750 2242 2504 835
Size: 915 492 262 2011
Fragments arranged by size:
2011 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3680
SphI G_CATG'C
Cuts at: 733 1233 733
Size: 500 3180
StyI C'CwwG_G
Cuts at: 107 298 814 1168 107
Size: 191 516 354 2619
Fragments arranged by size:
2619 516 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1263 1396 1855 2064 1263
Size: 133 459 209 2879
Fragments arranged by size:
2879 459 209 133
TatI w'GTAC_w
Cuts at: 3195 3195
Size: 3680
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 125 157 3197 3229 125
Size: 32 3040 32 576
Fragments arranged by size:
3040 576 32 32
Tth111I GACn'n_nGTC
Cuts at: 1477 1477
Size: 3680
XbaI T'CTAG_A
Cuts at: 711 711
Size: 3680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 370 1175 370
Size: 805 2875
XmaI C'CCGG_G
Cuts at: 700 700
Size: 3680
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PciI PflMI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI
BmtI BpmI BsgI BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PasI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI