Restriction map for
pF1KE0414
(Circular) (Six-base) MAPSORT of: pF1KE0414.seq Check: 6463 from: 1 to: 3671
pF1KE0414 3671 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 709 709
Size: 3671
Acc65I G'GTAC_C
Cuts at: 687 687
Size: 3671
AclI AA'CG_TT
Cuts at: 3511 3511
Size: 3671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 1496 1928 2675 2943 520
Size: 976 432 747 268 1248
Fragments arranged by size:
1248 976 747 432 268
AfeI AGC'GCT
Cuts at: 403 403
Size: 3671
AflIII A'CryG_T
Cuts at: 275 1023 2127 3104 275
Size: 748 1104 977 842
Fragments arranged by size:
1104 977 842 748
AgeI A'CCGG_T
Cuts at: 2059 2059
Size: 3671
AlwNI CAG_nnn'CTG
Cuts at: 976 2543 976
Size: 1567 2104
ApaLI G'TGCA_C
Cuts at: 454 2441 454
Size: 1987 1684
ApoI r'AATT_y
Cuts at: 675 3024 3280 675
Size: 2349 256 1066
Fragments arranged by size:
2349 1066 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3671
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
AvaI C'yCGr_G
Cuts at: 282 325 691 282
Size: 43 366 3262
Fragments arranged by size:
3262 366 43
BamHI G'GATC_C
Cuts at: 696 696
Size: 3671
BanI G'GyrC_C
Cuts at: 208 576 687 1101 1349 1384 208
Size: 368 111 414 248 35 2495
Fragments arranged by size:
2495 414 368 248 111 35
BanII G_rGCy'C
Cuts at: 632 685 1715 632
Size: 53 1030 2588
Fragments arranged by size:
2588 1030 53
BbeI G_GCGC'C
Cuts at: 212 1105 1353 212
Size: 893 248 2530
Fragments arranged by size:
2530 893 248
BciVI GTATCCnnnnn_n'
Cuts at: 1569 2330 3671 1569
Size: 761 1341 1569
Fragments arranged by size:
1569 1341 761
BclI T'GATC_A
Cuts at: 1191 1191
Size: 3671
BglII A'GATC_T
Cuts at: 1186 3223 1186
Size: 2037 1634
BlpI GC'TnA_GC
Cuts at: 783 783
Size: 3671
Bme1580I G_kGCm'C
Cuts at: 458 581 1296 1389 2445 458
Size: 123 715 93 1056 1684
Fragments arranged by size:
1684 1056 715 123 93
BmgBI CAC'GTC
Cuts at: 453 573 453
Size: 120 3551
BmrI ACTGGGnnnn_n'
Cuts at: 883 964 1299 3443 883
Size: 81 335 2144 1111
Fragments arranged by size:
2144 1111 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 360 2038 360
Size: 1678 1993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 448
Size: 3671
Bpu10I CC'TnA_GC
Cuts at: 415 415
Size: 3671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1726 2218 2516 847
Size: 879 492 298 2002
Fragments arranged by size:
2002 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3375 3375
Size: 3671
BsaAI yAC'GTr
Cuts at: 276 1024 276
Size: 748 2923
BsaBI GATnn'nnATC
Cuts at: 6 1185 3222 6
Size: 1179 2037 455
Fragments arranged by size:
2037 1179 455
BsaHI Gr'CG_yC
Cuts at: 209 1102 1350 2052 3548 209
Size: 893 248 702 1496 332
Fragments arranged by size:
1496 893 702 332 248
BsaWI w'CCGG_w
Cuts at: 860 1381 2059 2333 2480 2891 860
Size: 521 678 274 147 411 1640
Fragments arranged by size:
1640 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 186 186
Size: 3671
BseYI C'CCAG_C
Cuts at: 435 1096 1854 2431 2927 435
Size: 661 758 577 496 1179
Fragments arranged by size:
1179 758 661 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 517
Size: 3671
BsiEI CG_ry'CG
Cuts at: 104 548 1213 1259 2467 104
Size: 444 665 46 1208 1308
Fragments arranged by size:
1308 1208 665 444 46
BsiHKAI G_wGCw'C
Cuts at: 458 632 685 1463 1653 2445 458
Size: 174 53 778 190 792 1684
Fragments arranged by size:
1684 792 778 190 174 53
Bsp1286I G_dGCh'C
Cuts at: 458 581 632 685 1296 1389 1463 1653 1715
Size: 123 51 53 611 93 74 190 62
Cuts at: 1715 2445 458
Size: 730 1684
Fragments arranged by size:
1684 730 611 190 123 93 74 62
53 51
BspEI T'CCGG_A
Cuts at: 2891 2891
Size: 3671
BspHI T'CATG_A
Cuts at: 2847 2847
Size: 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 524 721 935 1237 1618 524
Size: 197 214 302 381 2577
Fragments arranged by size:
2577 381 302 214 197
BsrBI CCG'CTC
Cuts at: 50 1963 2017 3501 3666 50
Size: 1913 54 1484 165 55
Fragments arranged by size:
1913 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1583 1583
Size: 3671
BsrFI r'CCGG_y
Cuts at: 1669 1850 2059 1669
Size: 181 209 3281
Fragments arranged by size:
3281 209 181
BssHII G'CGCG_C
Cuts at: 223 419 421 519 223
Size: 196 2 98 3375
Fragments arranged by size:
3375 196 98 2
BssSI C'ACGA_G
Cuts at: 1942 2300 1942
Size: 358 3313
BstAPI GCAn_nnn'nTGC
Cuts at: 1048 1048
Size: 3671
BstBI TT'CG_AA
Cuts at: 2032 2032
Size: 3671
BstXI CCAn_nnnn'nTGG
Cuts at: 357 591 357
Size: 234 3437
BstYI r'GATC_y
Cuts at: 1 191 696 1163 1186 1521 1767 2779 2865
Size: 190 505 467 23 335 246 1012 86
Cuts at: 2865 2877 3223 1
Size: 12 346 449
Fragments arranged by size:
1012 505 467 449 346 335 246 190
86 23 12
BtgI C'CryG_G
Cuts at: 107 368 107
Size: 261 3410
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1621 1802 1965 1621
Size: 181 163 3327
Fragments arranged by size:
3327 181 163
BtsI GCAGTG_nn'
Cuts at: 914 914
Size: 3671
DraI TTT'AAA
Cuts at: 670 670
Size: 3671
DrdI GACnn_nn'nnGTC
Cuts at: 1377 2235 1377
Size: 858 2813
EaeI y'GGCC_r
Cuts at: 314 545 1256 1430 1821 1848 3193 314
Size: 231 711 174 391 27 1345 792
Fragments arranged by size:
1345 792 711 391 231 174 27
EagI C'GGCC_G
Cuts at: 545 1256 545
Size: 711 2960
EarI CTCTTCn'nnn_
Cuts at: 661 1694 1904 661
Size: 1033 210 2428
Fragments arranged by size:
2428 1033 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1879 2189 2335 3481 1879
Size: 310 146 1146 2069
Fragments arranged by size:
2069 1146 310 146
EcoICRI GAG'CTC
Cuts at: 630 683 630
Size: 53 3618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 520 1496 1928 2675 2943 448
Size: 72 976 432 747 268 1176
Fragments arranged by size:
1176 976 747 432 268 72
EcoO109I rG'GnC_Cy
Cuts at: 810 810
Size: 3671
EcoRI G'AATT_C
Cuts at: 675 675
Size: 3671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 672 1690 672
Size: 1018 2653
FspI TGC'GCA
Cuts at: 556 1452 556
Size: 896 2775
HaeII r_GCGC'y
Cuts at: 212 405 1105 1353 2375 3354 212
Size: 193 700 248 1022 979 529
Fragments arranged by size:
1022 979 700 529 248 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1513 1543 1545 1575 1513
Size: 30 2 30 3609
Fragments arranged by size:
3609 30 30 2
HincII GTy'rAC
Cuts at: 710 710
Size: 3671
KasI G'GCGC_C
Cuts at: 208 1101 1349 208
Size: 893 248 2530
Fragments arranged by size:
2530 893 248
KpnI G_GTAC'C
Cuts at: 691 691
Size: 3671
MluI A'CGCG_T
Cuts at: 3104 3104
Size: 3671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2342 2526 2342
Size: 184 3487
MscI TGG'CCA
Cuts at: 316 1432 316
Size: 1116 2555
MslI CAynn'nnrTG
Cuts at: 571 1660 1787 571
Size: 1089 127 2455
Fragments arranged by size:
2455 1089 127
MspA1I CmG'CkG
Cuts at: 252 507 782 1096 1456 2469 2714 252
Size: 255 275 314 360 1013 245 1209
Fragments arranged by size:
1209 1013 360 314 275 255 245
NaeI GCC'GGC
Cuts at: 1852 1852
Size: 3671
NarI GG'CG_CC
Cuts at: 209 1102 1350 209
Size: 893 248 2530
Fragments arranged by size:
2530 893 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3671
NgoMIV G'CCGG_C
Cuts at: 1850 1850
Size: 3671
NspI r_CATG'y
Cuts at: 724 1224 2131 724
Size: 500 907 2264
Fragments arranged by size:
2264 907 500
PciI A'CATG_T
Cuts at: 2127 2127
Size: 3671
PmeI GTTT'AAAC
Cuts at: 670 670
Size: 3671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2810 2842 2810
Size: 32 3639
PstI C_TGCA'G
Cuts at: 718 1403 718
Size: 685 2986
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3671
PvuII CAG'CTG
Cuts at: 252 1096 1456 252
Size: 844 360 2467
Fragments arranged by size:
2467 844 360
RsrII CG'GwC_CG
Cuts at: 1866 1866
Size: 3671
SacI G_AGCT'C
Cuts at: 632 685 632
Size: 53 3618
SalI G'TCGA_C
Cuts at: 708 708
Size: 3671
SapI GCTCTTCn'nnn_
Cuts at: 1694 1904 1694
Size: 210 3461
SbfI CC_TGCA'GG
Cuts at: 718 718
Size: 3671
ScaI AGT'ACT
Cuts at: 3188 3188
Size: 3671
SfcI C'TryA_G
Cuts at: 33 441 714 1399 2392 2583 33
Size: 408 273 685 993 191 1121
Fragments arranged by size:
1121 993 685 408 273 191
SfoI GGC'GCC
Cuts at: 210 1103 1351 210
Size: 893 248 2530
Fragments arranged by size:
2530 893 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
SmaI CCC'GGG
Cuts at: 284 693 284
Size: 409 3262
SmlI C'TyrA_G
Cuts at: 826 1741 2233 2495 826
Size: 915 492 262 2002
Fragments arranged by size:
2002 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3671
SphI G_CATG'C
Cuts at: 724 1224 724
Size: 500 3171
StyI C'CwwG_G
Cuts at: 107 635 805 1159 107
Size: 528 170 354 2619
Fragments arranged by size:
2619 528 354 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1254 1387 1846 2055 1254
Size: 133 459 209 2870
Fragments arranged by size:
2870 459 209 133
TatI w'GTAC_w
Cuts at: 3186 3186
Size: 3671
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 3639
Tth111I GACn'n_nGTC
Cuts at: 1468 1468
Size: 3671
XbaI T'CTAG_A
Cuts at: 702 702
Size: 3671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 354 1166 354
Size: 812 2859
XmaI C'CCGG_G
Cuts at: 282 691 282
Size: 409 3262
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI