Restriction map for
pF1KE0410
(Circular) (Six-base) MAPSORT of: pF1KE0410.seq Check: 6474 from: 1 to: 3668
pF1KE0410 3668 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 603 603
Size: 3668
AccI GT'mk_AC
Cuts at: 706 706
Size: 3668
Acc65I G'GTAC_C
Cuts at: 684 684
Size: 3668
AclI AA'CG_TT
Cuts at: 3508 3508
Size: 3668
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 200 1493 1925 2672 2940 182
Size: 18 1293 432 747 268 910
Fragments arranged by size:
1293 910 747 432 268 18
AflIII A'CryG_T
Cuts at: 1020 2124 3101 1020
Size: 1104 977 1587
Fragments arranged by size:
1587 1104 977
AgeI A'CCGG_T
Cuts at: 2056 2056
Size: 3668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 295 295
Size: 3668
AlwNI CAG_nnn'CTG
Cuts at: 307 535 538 973 2540 307
Size: 228 3 435 1567 1435
Fragments arranged by size:
1567 1435 435 228 3
ApaLI G'TGCA_C
Cuts at: 2438 2438
Size: 3668
ApoI r'AATT_y
Cuts at: 672 3021 3277 672
Size: 2349 256 1063
Fragments arranged by size:
2349 1063 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3668
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
AvaI C'yCGr_G
Cuts at: 547 557 688 547
Size: 10 131 3527
Fragments arranged by size:
3527 131 10
BamHI G'GATC_C
Cuts at: 693 693
Size: 3668
BanI G'GyrC_C
Cuts at: 150 412 589 684 1098 1346 1381 150
Size: 262 177 95 414 248 35 2437
Fragments arranged by size:
2437 414 262 248 177 95 35
BanII G_rGCy'C
Cuts at: 296 682 1712 296
Size: 386 1030 2252
Fragments arranged by size:
2252 1030 386
BbeI G_GCGC'C
Cuts at: 1102 1350 1102
Size: 248 3420
BbsI GAAGACnn'nnnn_
Cuts at: 630 637 630
Size: 7 3661
BciVI GTATCCnnnnn_n'
Cuts at: 261 1566 2327 3668 261
Size: 1305 761 1341 261
Fragments arranged by size:
1341 1305 761 261
BclI T'GATC_A
Cuts at: 1188 1188
Size: 3668
BfrBI ATG'CAT
Cuts at: 200 475 200
Size: 275 3393
BglII A'GATC_T
Cuts at: 1183 3220 1183
Size: 2037 1631
BlpI GC'TnA_GC
Cuts at: 658 780 658
Size: 122 3546
Bme1580I G_kGCm'C
Cuts at: 155 233 417 594 1293 1386 2442 155
Size: 78 184 177 699 93 1056 1381
Fragments arranged by size:
1381 1056 699 184 177 93 78
BmgBI CAC'GTC
Cuts at: 318 318
Size: 3668
BmrI ACTGGGnnnn_n'
Cuts at: 880 961 1296 3440 880
Size: 81 335 2144 1108
Fragments arranged by size:
2144 1108 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 3668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 595
Size: 3668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1723 2215 2513 844
Size: 879 492 298 1999
Fragments arranged by size:
1999 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3372 3372
Size: 3668
BsaAI yAC'GTr
Cuts at: 285 1021 285
Size: 736 2932
BsaBI GATnn'nnATC
Cuts at: 6 1182 3219 6
Size: 1176 2037 455
Fragments arranged by size:
2037 1176 455
BsaHI Gr'CG_yC
Cuts at: 1099 1347 2049 3545 1099
Size: 248 702 1496 1222
Fragments arranged by size:
1496 1222 702 248
BsaWI w'CCGG_w
Cuts at: 188 857 1378 2056 2330 2477 2888 188
Size: 669 521 678 274 147 411 968
Fragments arranged by size:
968 678 669 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 370 514 517 370
Size: 144 3 3521
Fragments arranged by size:
3521 144 3
BseYI C'CCAG_C
Cuts at: 156 593 1093 1851 2428 2924 156
Size: 437 500 758 577 496 900
Fragments arranged by size:
900 758 577 500 496 437
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 366 475 637 265
Size: 101 109 162 3296
Fragments arranged by size:
3296 162 109 101
BsiEI CG_ry'CG
Cuts at: 104 645 1210 1256 2464 104
Size: 541 565 46 1208 1308
Fragments arranged by size:
1308 1208 565 541 46
BsiHKAI G_wGCw'C
Cuts at: 682 1460 1650 2442 682
Size: 778 190 792 1908
Fragments arranged by size:
1908 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 155 233 296 417 594 682 1293 1386 1460
Size: 78 63 121 177 88 611 93 74
Cuts at: 1460 1650 1712 2442 155
Size: 190 62 730 1381
Fragments arranged by size:
1381 730 611 190 177 121 93 88
78 74 63 62
BspEI T'CCGG_A
Cuts at: 2888 2888
Size: 3668
BspHI T'CATG_A
Cuts at: 2844 2844
Size: 3668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 338 603 718 932 1234 1615 338
Size: 265 115 214 302 381 2391
Fragments arranged by size:
2391 381 302 265 214 115
BsrBI CCG'CTC
Cuts at: 50 1960 2014 3498 3663 50
Size: 1910 54 1484 165 55
Fragments arranged by size:
1910 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1580 1580
Size: 3668
BsrFI r'CCGG_y
Cuts at: 1666 1847 2056 1666
Size: 181 209 3278
Fragments arranged by size:
3278 209 181
BssSI C'ACGA_G
Cuts at: 1939 2297 1939
Size: 358 3310
BstAPI GCAn_nnn'nTGC
Cuts at: 535 538 654 1045 535
Size: 3 116 391 3158
Fragments arranged by size:
3158 391 116 3
BstBI TT'CG_AA
Cuts at: 2029 2029
Size: 3668
BstYI r'GATC_y
Cuts at: 1 693 1160 1183 1518 1764 2776 2862 2874
Size: 692 467 23 335 246 1012 86 12
Cuts at: 2874 3220 1
Size: 346 449
Fragments arranged by size:
1012 692 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 404 404
Size: 3668
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1618 1799 1962 1618
Size: 181 163 3324
Fragments arranged by size:
3324 181 163
BtsI GCAGTG_nn'
Cuts at: 911 911
Size: 3668
DraI TTT'AAA
Cuts at: 667 667
Size: 3668
DraIII CAC_nnn'GTG
Cuts at: 288 288
Size: 3668
DrdI GACnn_nn'nnGTC
Cuts at: 1374 2232 1374
Size: 858 2810
EaeI y'GGCC_r
Cuts at: 309 1253 1427 1818 1845 3190 309
Size: 944 174 391 27 1345 787
Fragments arranged by size:
1345 944 787 391 174 27
EagI C'GGCC_G
Cuts at: 1253 1253
Size: 3668
EarI CTCTTCn'nnn_
Cuts at: 1691 1901 1691
Size: 210 3458
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1876 2186 2332 3478 1876
Size: 310 146 1146 2066
Fragments arranged by size:
2066 1146 310 146
EcoICRI GAG'CTC
Cuts at: 680 680
Size: 3668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 200 595 1493 1925 2672 2940 182
Size: 18 395 898 432 747 268 910
Fragments arranged by size:
910 898 747 432 395 268 18
EcoO109I rG'GnC_Cy
Cuts at: 553 807 553
Size: 254 3414
EcoRI G'AATT_C
Cuts at: 672 672
Size: 3668
EcoRV GAT'ATC
Cuts at: 186 186
Size: 3668
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 654 1687 654
Size: 1033 2635
FspI TGC'GCA
Cuts at: 1449 1449
Size: 3668
HaeII r_GCGC'y
Cuts at: 1102 1350 2372 3351 1102
Size: 248 1022 979 1419
Fragments arranged by size:
1419 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 505 537 1510 1540 1542 1572 505
Size: 32 973 30 2 30 2601
Fragments arranged by size:
2601 973 32 30 30 2
HincII GTy'rAC
Cuts at: 707 707
Size: 3668
KasI G'GCGC_C
Cuts at: 1098 1346 1098
Size: 248 3420
KpnI G_GTAC'C
Cuts at: 688 688
Size: 3668
MluI A'CGCG_T
Cuts at: 3101 3101
Size: 3668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2339 2523 2339
Size: 184 3484
MscI TGG'CCA
Cuts at: 311 1429 311
Size: 1118 2550
MslI CAynn'nnrTG
Cuts at: 301 1657 1784 301
Size: 1356 127 2185
Fragments arranged by size:
2185 1356 127
MspA1I CmG'CkG
Cuts at: 160 535 600 627 779 1093 1453 2466 2711
Size: 375 65 27 152 314 360 1013 245
Cuts at: 2711 160
Size: 1117
Fragments arranged by size:
1117 1013 375 360 314 245 152 65
27
NaeI GCC'GGC
Cuts at: 1849 1849
Size: 3668
NarI GG'CG_CC
Cuts at: 1099 1347 1099
Size: 248 3420
NgoMIV G'CCGG_C
Cuts at: 1847 1847
Size: 3668
NsiI A_TGCA'T
Cuts at: 202 477 202
Size: 275 3393
NspI r_CATG'y
Cuts at: 721 1221 2128 721
Size: 500 907 2261
Fragments arranged by size:
2261 907 500
PciI A'CATG_T
Cuts at: 2124 2124
Size: 3668
PflMI CCAn_nnn'nTGG
Cuts at: 307 318 307
Size: 11 3657
PfoI T'CCnGG_A
Cuts at: 572 572
Size: 3668
PmeI GTTT'AAAC
Cuts at: 667 667
Size: 3668
PmlI CAC'GTG
Cuts at: 285 285
Size: 3668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2807 2839 2807
Size: 32 3636
PpuMI rG'GwC_Cy
Cuts at: 553 553
Size: 3668
PstI C_TGCA'G
Cuts at: 614 715 1400 614
Size: 101 685 2882
Fragments arranged by size:
2882 685 101
PvuI CG_AT'CG
Cuts at: 104 645 104
Size: 541 3127
PvuII CAG'CTG
Cuts at: 160 535 1093 1453 160
Size: 375 558 360 2375
Fragments arranged by size:
2375 558 375 360
RsrII CG'GwC_CG
Cuts at: 1863 1863
Size: 3668
SacI G_AGCT'C
Cuts at: 682 682
Size: 3668
SalI G'TCGA_C
Cuts at: 705 705
Size: 3668
SanDI GG'GwC_CC
Cuts at: 553 553
Size: 3668
SapI GCTCTTCn'nnn_
Cuts at: 1691 1901 1691
Size: 210 3458
SbfI CC_TGCA'GG
Cuts at: 715 715
Size: 3668
ScaI AGT'ACT
Cuts at: 3185 3185
Size: 3668
SfcI C'TryA_G
Cuts at: 33 610 711 1396 2389 2580 33
Size: 577 101 685 993 191 1121
Fragments arranged by size:
1121 993 685 577 191 101
SfoI GGC'GCC
Cuts at: 1100 1348 1100
Size: 248 3420
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
SmaI CCC'GGG
Cuts at: 690 690
Size: 3668
SmlI C'TyrA_G
Cuts at: 823 1738 2230 2492 823
Size: 915 492 262 1999
Fragments arranged by size:
1999 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3668
SphI G_CATG'C
Cuts at: 721 1221 721
Size: 500 3168
StuI AGG'CCT
Cuts at: 325 325
Size: 3668
StyI C'CwwG_G
Cuts at: 802 1156 802
Size: 354 3314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 400 1251 1384 1843 2052 400
Size: 851 133 459 209 2016
Fragments arranged by size:
2016 851 459 209 133
TatI w'GTAC_w
Cuts at: 3183 3183
Size: 3668
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 3636
Tth111I GACn'n_nGTC
Cuts at: 1465 1465
Size: 3668
XbaI T'CTAG_A
Cuts at: 699 699
Size: 3668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1163 1163
Size: 3668
XmaI C'CCGG_G
Cuts at: 688 688
Size: 3668
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI PacI PasI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI