Restriction map for
pF1KE0409
(Circular) (Six-base) MAPSORT of: pF1KE0409.seq Check: 3428 from: 1 to: 3698
pF1KE0409 3698 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 300 736 300
Size: 436 3262
Acc65I G'GTAC_C
Cuts at: 714 714
Size: 3698
AclI AA'CG_TT
Cuts at: 3538 3538
Size: 3698
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 581 1523 1955 2702 2970 251
Size: 330 942 432 747 268 979
Fragments arranged by size:
979 942 747 432 330 268
AflIII A'CryG_T
Cuts at: 1050 2154 3131 1050
Size: 1104 977 1617
Fragments arranged by size:
1617 1104 977
AgeI A'CCGG_T
Cuts at: 2086 2086
Size: 3698
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 253 285 253
Size: 32 3666
AlwNI CAG_nnn'CTG
Cuts at: 466 1003 2570 466
Size: 537 1567 1594
Fragments arranged by size:
1594 1567 537
ApaI G_GGCC'C
Cuts at: 342 342
Size: 3698
ApaLI G'TGCA_C
Cuts at: 2468 2468
Size: 3698
ApoI r'AATT_y
Cuts at: 215 702 3051 3307 215
Size: 487 2349 256 606
Fragments arranged by size:
2349 606 487 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3698
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
AvaI C'yCGr_G
Cuts at: 718 718
Size: 3698
BamHI G'GATC_C
Cuts at: 723 723
Size: 3698
BanI G'GyrC_C
Cuts at: 681 714 1128 1376 1411 681
Size: 33 414 248 35 2968
Fragments arranged by size:
2968 414 248 35 33
BanII G_rGCy'C
Cuts at: 134 342 712 1742 134
Size: 208 370 1030 2090
Fragments arranged by size:
2090 1030 370 208
BbeI G_GCGC'C
Cuts at: 1132 1380 1132
Size: 248 3450
BbsI GAAGACnn'nnnn_
Cuts at: 624 624
Size: 3698
BbvCI CC'TCA_GC
Cuts at: 451 451
Size: 3698
BciVI GTATCCnnnnn_n'
Cuts at: 1596 2357 3698 1596
Size: 761 1341 1596
Fragments arranged by size:
1596 1341 761
BclI T'GATC_A
Cuts at: 1218 1218
Size: 3698
BglII A'GATC_T
Cuts at: 1213 3250 1213
Size: 2037 1661
BlpI GC'TnA_GC
Cuts at: 181 810 181
Size: 629 3069
Bme1580I G_kGCm'C
Cuts at: 342 1323 1416 2472 342
Size: 981 93 1056 1568
Fragments arranged by size:
1568 1056 981 93
BmrI ACTGGGnnnn_n'
Cuts at: 336 910 991 1326 3470 336
Size: 574 81 335 2144 564
Fragments arranged by size:
2144 574 564 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 150 2065 150
Size: 1915 1783
Bpu10I CC'TnA_GC
Cuts at: 451 451
Size: 3698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 661 874 1753 2245 2543 391
Size: 270 213 879 492 298 1546
Fragments arranged by size:
1546 879 492 298 270 213
BsaI GGTCTCn'nnnn_
Cuts at: 3402 3402
Size: 3698
BsaAI yAC'GTr
Cuts at: 1051 1051
Size: 3698
BsaBI GATnn'nnATC
Cuts at: 6 1212 3249 6
Size: 1206 2037 455
Fragments arranged by size:
2037 1206 455
BsaHI Gr'CG_yC
Cuts at: 1129 1377 2079 3575 1129
Size: 248 702 1496 1252
Fragments arranged by size:
1496 1252 702 248
BsaWI w'CCGG_w
Cuts at: 887 1408 2086 2360 2507 2918 887
Size: 521 678 274 147 411 1667
Fragments arranged by size:
1667 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 142
Size: 3698
BseYI C'CCAG_C
Cuts at: 1123 1881 2458 2954 1123
Size: 758 577 496 1867
Fragments arranged by size:
1867 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 142
Size: 3698
BsiEI CG_ry'CG
Cuts at: 104 1240 1286 2494 104
Size: 1136 46 1208 1308
Fragments arranged by size:
1308 1208 1136 46
BsiHKAI G_wGCw'C
Cuts at: 134 712 1490 1680 2472 134
Size: 578 778 190 792 1360
Fragments arranged by size:
1360 792 778 578 190
Bsp1286I G_dGCh'C
Cuts at: 134 342 712 1323 1416 1490 1680 1742 2472
Size: 208 370 611 93 74 190 62 730
Cuts at: 2472 134
Size: 1360
Fragments arranged by size:
1360 730 611 370 208 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2918 2918
Size: 3698
BspHI T'CATG_A
Cuts at: 408 2874 408
Size: 2466 1232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 748 962 1264 1645 748
Size: 214 302 381 2801
Fragments arranged by size:
2801 381 302 214
BsrBI CCG'CTC
Cuts at: 50 188 1990 2044 3528 3693 50
Size: 138 1802 54 1484 165 55
Fragments arranged by size:
1802 1484 165 138 55 54
BsrDI GCAATG_nn'
Cuts at: 1610 1610
Size: 3698
BsrFI r'CCGG_y
Cuts at: 1696 1877 2086 1696
Size: 181 209 3308
Fragments arranged by size:
3308 209 181
BssSI C'ACGA_G
Cuts at: 166 1969 2327 166
Size: 1803 358 1537
Fragments arranged by size:
1803 1537 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1075 1075
Size: 3698
BstBI TT'CG_AA
Cuts at: 2059 2059
Size: 3698
BstYI r'GATC_y
Cuts at: 1 723 1190 1213 1548 1794 2806 2892 2904
Size: 722 467 23 335 246 1012 86 12
Cuts at: 2904 3250 1
Size: 346 449
Fragments arranged by size:
1012 722 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 509 107
Size: 402 3296
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1648 1829 1992 1648
Size: 181 163 3354
Fragments arranged by size:
3354 181 163
BtsI GCAGTG_nn'
Cuts at: 477 483 941 477
Size: 6 458 3234
Fragments arranged by size:
3234 458 6
DraI TTT'AAA
Cuts at: 697 697
Size: 3698
DrdI GACnn_nn'nnGTC
Cuts at: 1404 2262 1404
Size: 858 2840
EaeI y'GGCC_r
Cuts at: 143 1283 1457 1848 1875 3220 143
Size: 1140 174 391 27 1345 621
Fragments arranged by size:
1345 1140 621 391 174 27
EagI C'GGCC_G
Cuts at: 1283 1283
Size: 3698
EarI CTCTTCn'nnn_
Cuts at: 1721 1931 1721
Size: 210 3488
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1906 2216 2362 3508 127
Size: 1779 310 146 1146 317
Fragments arranged by size:
1779 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 132 710 132
Size: 578 3120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 581 1523 1955 2702 2970 251
Size: 330 942 432 747 268 979
Fragments arranged by size:
979 942 747 432 330 268
EcoO109I rG'GnC_Cy
Cuts at: 161 338 837 161
Size: 177 499 3022
Fragments arranged by size:
3022 499 177
EcoRI G'AATT_C
Cuts at: 215 702 215
Size: 487 3211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1717 1717
Size: 3698
FspI TGC'GCA
Cuts at: 1479 1479
Size: 3698
HaeII r_GCGC'y
Cuts at: 1132 1380 2402 3381 1132
Size: 248 1022 979 1449
Fragments arranged by size:
1449 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1540 1570 1572 1602 1540
Size: 30 2 30 3636
Fragments arranged by size:
3636 30 30 2
HincII GTy'rAC
Cuts at: 332 737 332
Size: 405 3293
KasI G'GCGC_C
Cuts at: 1128 1376 1128
Size: 248 3450
KpnI G_GTAC'C
Cuts at: 718 718
Size: 3698
MluI A'CGCG_T
Cuts at: 3131 3131
Size: 3698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 374 2369 2553 374
Size: 1995 184 1519
Fragments arranged by size:
1995 1519 184
MscI TGG'CCA
Cuts at: 1459 1459
Size: 3698
MslI CAynn'nnrTG
Cuts at: 508 1687 1814 508
Size: 1179 127 2392
Fragments arranged by size:
2392 1179 127
MspA1I CmG'CkG
Cuts at: 809 1123 1483 2496 2741 809
Size: 314 360 1013 245 1766
Fragments arranged by size:
1766 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1879 1879
Size: 3698
NarI GG'CG_CC
Cuts at: 1129 1377 1129
Size: 248 3450
NcoI C'CATG_G
Cuts at: 107 509 107
Size: 402 3296
NgoMIV G'CCGG_C
Cuts at: 1877 1877
Size: 3698
NspI r_CATG'y
Cuts at: 751 1251 2158 751
Size: 500 907 2291
Fragments arranged by size:
2291 907 500
PciI A'CATG_T
Cuts at: 2154 2154
Size: 3698
PflMI CCAn_nnn'nTGG
Cuts at: 466 466
Size: 3698
PmeI GTTT'AAAC
Cuts at: 697 697
Size: 3698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 3666
PspOMI G'GGCC_C
Cuts at: 338 338
Size: 3698
PstI C_TGCA'G
Cuts at: 160 745 1430 160
Size: 585 685 2428
Fragments arranged by size:
2428 685 585
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3698
PvuII CAG'CTG
Cuts at: 1123 1483 1123
Size: 360 3338
RsrII CG'GwC_CG
Cuts at: 1893 1893
Size: 3698
SacI G_AGCT'C
Cuts at: 134 712 134
Size: 578 3120
SalI G'TCGA_C
Cuts at: 735 735
Size: 3698
SapI GCTCTTCn'nnn_
Cuts at: 1721 1931 1721
Size: 210 3488
SbfI CC_TGCA'GG
Cuts at: 745 745
Size: 3698
ScaI AGT'ACT
Cuts at: 3215 3215
Size: 3698
SexAI A'CCwGG_T
Cuts at: 303 303
Size: 3698
SfcI C'TryA_G
Cuts at: 33 156 655 741 1426 2419 2610 33
Size: 123 499 86 685 993 191 1121
Fragments arranged by size:
1121 993 685 499 191 123 86
SfoI GGC'GCC
Cuts at: 1130 1378 1130
Size: 248 3450
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
SmaI CCC'GGG
Cuts at: 720 720
Size: 3698
SmlI C'TyrA_G
Cuts at: 370 676 853 1768 2260 2522 370
Size: 306 177 915 492 262 1546
Fragments arranged by size:
1546 915 492 306 262 177
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3698
SphI G_CATG'C
Cuts at: 751 1251 751
Size: 500 3198
StyI C'CwwG_G
Cuts at: 107 509 832 1186 107
Size: 402 323 354 2619
Fragments arranged by size:
2619 402 354 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 126 1281 1414 1873 2082 126
Size: 1155 133 459 209 1742
Fragments arranged by size:
1742 1155 459 209 133
TatI w'GTAC_w
Cuts at: 487 3213 487
Size: 2726 972
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 3666
Tth111I GACn'n_nGTC
Cuts at: 1495 1495
Size: 3698
XbaI T'CTAG_A
Cuts at: 729 729
Size: 3698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 463 1193 463
Size: 730 2968
XmaI C'CCGG_G
Cuts at: 718 718
Size: 3698
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BpmI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI