Restriction map for
pF1KE0408
(Circular) (Six-base) MAPSORT of: pF1KE0408.seq Check: 2043 from: 1 to: 4100
pF1KE0408 4100 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 318 318
Size: 4100
AccI GT'mk_AC
Cuts at: 755 1138 755
Size: 383 3717
Acc65I G'GTAC_C
Cuts at: 931 1116 931
Size: 185 3915
AclI AA'CG_TT
Cuts at: 3940 3940
Size: 4100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 221 287 1925 2357 3104 3372 158
Size: 63 66 1638 432 747 268 886
Fragments arranged by size:
1638 886 747 432 268 66 63
AflIII A'CryG_T
Cuts at: 1452 2556 3533 1452
Size: 1104 977 2019
Fragments arranged by size:
2019 1104 977
AgeI A'CCGG_T
Cuts at: 2488 2488
Size: 4100
AlwNI CAG_nnn'CTG
Cuts at: 346 1405 2972 346
Size: 1059 1567 1474
Fragments arranged by size:
1567 1474 1059
ApaLI G'TGCA_C
Cuts at: 2870 2870
Size: 4100
ApoI r'AATT_y
Cuts at: 356 1104 3453 3709 356
Size: 748 2349 256 747
Fragments arranged by size:
2349 748 747 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4100
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
AvaI C'yCGr_G
Cuts at: 1120 1120
Size: 4100
BamHI G'GATC_C
Cuts at: 1125 1125
Size: 4100
BanI G'GyrC_C
Cuts at: 330 931 1116 1530 1778 1813 330
Size: 601 185 414 248 35 2617
Fragments arranged by size:
2617 601 414 248 185 35
BanII G_rGCy'C
Cuts at: 125 605 800 1114 2144 125
Size: 480 195 314 1030 2081
Fragments arranged by size:
2081 1030 480 314 195
BbeI G_GCGC'C
Cuts at: 1534 1782 1534
Size: 248 3852
BbvCI CC'TCA_GC
Cuts at: 209 209
Size: 4100
BciVI GTATCCnnnnn_n'
Cuts at: 915 1998 2759 4100 915
Size: 1083 761 1341 915
Fragments arranged by size:
1341 1083 915 761
BclI T'GATC_A
Cuts at: 1620 1620
Size: 4100
BglII A'GATC_T
Cuts at: 1615 3652 1615
Size: 2037 2063
BlpI GC'TnA_GC
Cuts at: 1212 1212
Size: 4100
Bme1580I G_kGCm'C
Cuts at: 231 335 823 1725 1818 2874 231
Size: 104 488 902 93 1056 1457
Fragments arranged by size:
1457 1056 902 488 104 93
BmrI ACTGGGnnnn_n'
Cuts at: 774 1312 1393 1728 3872 774
Size: 538 81 335 2144 1002
Fragments arranged by size:
2144 1002 538 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 180 651 2467 180
Size: 471 1816 1813
Fragments arranged by size:
1816 1813 471
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 646 994 1075 457
Size: 189 348 81 3482
Fragments arranged by size:
3482 348 189 81
Bpu10I CC'TnA_GC
Cuts at: 209 209
Size: 4100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 2155 2647 2945 1276
Size: 879 492 298 2431
Fragments arranged by size:
2431 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3804 3804
Size: 4100
BsaAI yAC'GTr
Cuts at: 1453 1453
Size: 4100
BsaBI GATnn'nnATC
Cuts at: 6 1614 3651 6
Size: 1608 2037 455
Fragments arranged by size:
2037 1608 455
BsaHI Gr'CG_yC
Cuts at: 1531 1779 2481 3977 1531
Size: 248 702 1496 1654
Fragments arranged by size:
1654 1496 702 248
BsaWI w'CCGG_w
Cuts at: 696 1289 1810 2488 2762 2909 3320 696
Size: 593 521 678 274 147 411 1476
Fragments arranged by size:
1476 678 593 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 160 163 172 880 889 892 1072 1075
Size: 3 3 9 708 9 3 180 3
Cuts at: 1075 157
Size: 3182
Fragments arranged by size:
3182 708 180 9 9 3 3 3
3
BseYI C'CCAG_C
Cuts at: 124 961 1525 2283 2860 3356 124
Size: 837 564 758 577 496 868
Fragments arranged by size:
868 837 758 577 564 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 733
Size: 4100
BsiEI CG_ry'CG
Cuts at: 104 1642 1688 2896 104
Size: 1538 46 1208 1308
Fragments arranged by size:
1538 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 164 605 1114 1892 2082 2874 164
Size: 441 509 778 190 792 1390
Fragments arranged by size:
1390 792 778 509 441 190
BsmBI CGTCTCn'nnnn_
Cuts at: 622 622
Size: 4100
Bsp1286I G_dGCh'C
Cuts at: 125 164 231 335 605 800 823 1114 1725
Size: 39 67 104 270 195 23 291 611
Cuts at: 1725 1818 1892 2082 2144 2874 125
Size: 93 74 190 62 730 1351
Fragments arranged by size:
1351 730 611 291 270 195 190 104
93 74 67 62 39 23
BspEI T'CCGG_A
Cuts at: 3320 3320
Size: 4100
BspHI T'CATG_A
Cuts at: 3276 3276
Size: 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 318 1150 1364 1666 2047 318
Size: 832 214 302 381 2371
Fragments arranged by size:
2371 832 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2392 2446 3930 4095 50
Size: 2342 54 1484 165 55
Fragments arranged by size:
2342 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2012 2012
Size: 4100
BsrFI r'CCGG_y
Cuts at: 310 2098 2279 2488 310
Size: 1788 181 209 1922
Fragments arranged by size:
1922 1788 209 181
BssSI C'ACGA_G
Cuts at: 532 2371 2729 532
Size: 1839 358 1903
Fragments arranged by size:
1903 1839 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1477 1477
Size: 4100
BstBI TT'CG_AA
Cuts at: 2461 2461
Size: 4100
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4100
BstYI r'GATC_y
Cuts at: 1 1125 1592 1615 1950 2196 3208 3294 3306
Size: 1124 467 23 335 246 1012 86 12
Cuts at: 3306 3652 1
Size: 346 449
Fragments arranged by size:
1124 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 347 347
Size: 4100
BtgI C'CryG_G
Cuts at: 107 107
Size: 4100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2050 2231 2394 2050
Size: 181 163 3756
Fragments arranged by size:
3756 181 163
BtsI GCAGTG_nn'
Cuts at: 424 1343 424
Size: 919 3181
DraI TTT'AAA
Cuts at: 1099 1099
Size: 4100
DrdI GACnn_nn'nnGTC
Cuts at: 1806 2664 1806
Size: 858 3242
EaeI y'GGCC_r
Cuts at: 1685 1859 2250 2277 3622 1685
Size: 174 391 27 1345 2163
Fragments arranged by size:
2163 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1685 1685
Size: 4100
EarI CTCTTCn'nnn_
Cuts at: 134 149 2123 2333 134
Size: 15 1974 210 1901
Fragments arranged by size:
1974 1901 210 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2308 2618 2764 3910 2308
Size: 310 146 1146 2498
Fragments arranged by size:
2498 1146 310 146
EcoICRI GAG'CTC
Cuts at: 603 1112 603
Size: 509 3591
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 221 287 457 646 994 1075 1925 2357
Size: 63 66 170 189 348 81 850 432
Cuts at: 2357 3104 3372 158
Size: 747 268 886
Fragments arranged by size:
886 850 747 432 348 268 189 170
81 66 63
EcoNI CCTnn'n_nnAGG
Cuts at: 412 1054 412
Size: 642 3458
EcoO109I rG'GnC_Cy
Cuts at: 1239 1239
Size: 4100
EcoRI G'AATT_C
Cuts at: 1104 1104
Size: 4100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 4100
FspI TGC'GCA
Cuts at: 1881 1881
Size: 4100
HaeII r_GCGC'y
Cuts at: 1534 1782 2804 3783 1534
Size: 248 1022 979 1851
Fragments arranged by size:
1851 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 406 438 1942 1972 1974 2004 406
Size: 32 1504 30 2 30 2502
Fragments arranged by size:
2502 1504 32 30 30 2
HincII GTy'rAC
Cuts at: 375 1139 375
Size: 764 3336
KasI G'GCGC_C
Cuts at: 1530 1778 1530
Size: 248 3852
KpnI G_GTAC'C
Cuts at: 935 1120 935
Size: 185 3915
MluI A'CGCG_T
Cuts at: 3533 3533
Size: 4100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 614 868 2771 2955 614
Size: 254 1903 184 1759
Fragments arranged by size:
1903 1759 254 184
MscI TGG'CCA
Cuts at: 1861 1861
Size: 4100
MslI CAynn'nnrTG
Cuts at: 112 2089 2216 112
Size: 1977 127 1996
Fragments arranged by size:
1996 1977 127
MspA1I CmG'CkG
Cuts at: 1020 1211 1525 1885 2898 3143 1020
Size: 191 314 360 1013 245 1977
Fragments arranged by size:
1977 1013 360 314 245 191
NaeI GCC'GGC
Cuts at: 312 2281 312
Size: 1969 2131
NarI GG'CG_CC
Cuts at: 1531 1779 1531
Size: 248 3852
NcoI C'CATG_G
Cuts at: 107 107
Size: 4100
NdeI CA'TA_TG
Cuts at: 732 732
Size: 4100
NgoMIV G'CCGG_C
Cuts at: 310 2279 310
Size: 1969 2131
NspI r_CATG'y
Cuts at: 1153 1653 2560 1153
Size: 500 907 2693
Fragments arranged by size:
2693 907 500
PciI A'CATG_T
Cuts at: 2556 2556
Size: 4100
PmeI GTTT'AAAC
Cuts at: 1099 1099
Size: 4100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 4068
PstI C_TGCA'G
Cuts at: 1019 1147 1832 1019
Size: 128 685 3287
Fragments arranged by size:
3287 685 128
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4100
PvuII CAG'CTG
Cuts at: 1525 1885 1525
Size: 360 3740
RsrII CG'GwC_CG
Cuts at: 2295 2295
Size: 4100
SacI G_AGCT'C
Cuts at: 605 1114 605
Size: 509 3591
SalI G'TCGA_C
Cuts at: 1137 1137
Size: 4100
SapI GCTCTTCn'nnn_
Cuts at: 2123 2333 2123
Size: 210 3890
SbfI CC_TGCA'GG
Cuts at: 1147 1147
Size: 4100
ScaI AGT'ACT
Cuts at: 3617 3617
Size: 4100
SfcI C'TryA_G
Cuts at: 33 606 756 1015 1044 1143 1828 2821 3012
Size: 573 150 259 29 99 685 993 191
Cuts at: 3012 33
Size: 1121
Fragments arranged by size:
1121 993 685 573 259 191 150 99
29
SfoI GGC'GCC
Cuts at: 1532 1780 1532
Size: 248 3852
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
SmaI CCC'GGG
Cuts at: 1122 1122
Size: 4100
SmlI C'TyrA_G
Cuts at: 1255 2170 2662 2924 1255
Size: 915 492 262 2431
Fragments arranged by size:
2431 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4100
SphI G_CATG'C
Cuts at: 1153 1653 1153
Size: 500 3600
StyI C'CwwG_G
Cuts at: 107 367 926 1234 1588 107
Size: 260 559 308 354 2619
Fragments arranged by size:
2619 559 354 308 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1683 1816 2275 2484 1683
Size: 133 459 209 3299
Fragments arranged by size:
3299 459 209 133
TatI w'GTAC_w
Cuts at: 3615 3615
Size: 4100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3617 3649 3617
Size: 32 4068
Tth111I GACn'n_nGTC
Cuts at: 1897 1897
Size: 4100
XbaI T'CTAG_A
Cuts at: 1131 1131
Size: 4100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 651 1595 651
Size: 944 3156
XmaI C'CCGG_G
Cuts at: 1120 1120
Size: 4100
XmnI GAAnn'nnTTC
Cuts at: 811 811
Size: 4100
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI