Restriction map for
pF1KE0404
(Circular) (Six-base) MAPSORT of: pF1KE0404.seq Check: 7335 from: 1 to: 3935
pF1KE0404 3935 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AatII G_ACGT'C
Cuts at: 637 637
Size: 3935
AccI GT'mk_AC
Cuts at: 973 973
Size: 3935
Acc65I G'GTAC_C
Cuts at: 951 951
Size: 3935
AclI AA'CG_TT
Cuts at: 3775 3775
Size: 3935
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1760 2192 2939 3207 1760
Size: 432 747 268 2488
Fragments arranged by size:
2488 747 432 268
AflIII A'CryG_T
Cuts at: 162 1287 2391 3368 162
Size: 1125 1104 977 729
Fragments arranged by size:
1125 1104 977 729
AgeI A'CCGG_T
Cuts at: 2323 2323
Size: 3935
AhdI GACnn_n'nnGTC
Cuts at: 597 597
Size: 3935
AlwNI CAG_nnn'CTG
Cuts at: 723 804 1240 2807 723
Size: 81 436 1567 1851
Fragments arranged by size:
1851 1567 436 81
ApaLI G'TGCA_C
Cuts at: 2705 2705
Size: 3935
ApoI r'AATT_y
Cuts at: 298 559 939 3288 3544 298
Size: 261 380 2349 256 689
Fragments arranged by size:
2349 689 380 261 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3935
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
AvaI C'yCGr_G
Cuts at: 955 955
Size: 3935
BamHI G'GATC_C
Cuts at: 960 960
Size: 3935
BanI G'GyrC_C
Cuts at: 951 1365 1613 1648 951
Size: 414 248 35 3238
Fragments arranged by size:
3238 414 248 35
BanII G_rGCy'C
Cuts at: 455 949 1979 455
Size: 494 1030 2411
Fragments arranged by size:
2411 1030 494
BbeI G_GCGC'C
Cuts at: 1369 1617 1369
Size: 248 3687
BbvCI CC'TCA_GC
Cuts at: 787 787
Size: 3935
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3901
BciVI GTATCCnnnnn_n'
Cuts at: 1833 2594 3935 1833
Size: 761 1341 1833
Fragments arranged by size:
1833 1341 761
BclI T'GATC_A
Cuts at: 1455 1455
Size: 3935
BglII A'GATC_T
Cuts at: 1450 3487 1450
Size: 2037 1898
BlpI GC'TnA_GC
Cuts at: 155 1047 155
Size: 892 3043
Bme1580I G_kGCm'C
Cuts at: 1560 1653 2709 1560
Size: 93 1056 2786
Fragments arranged by size:
2786 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1147 1228 1563 3707 1147
Size: 81 335 2144 1375
Fragments arranged by size:
2144 1375 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 3935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 469 709 375
Size: 94 240 3601
Fragments arranged by size:
3601 240 94
Bpu10I CC'TnA_GC
Cuts at: 787 787
Size: 3935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1111 1990 2482 2780 1111
Size: 879 492 298 2266
Fragments arranged by size:
2266 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3639 3639
Size: 3935
BsaAI yAC'GTr
Cuts at: 1288 1288
Size: 3935
BsaBI GATnn'nnATC
Cuts at: 6 1449 3486 6
Size: 1443 2037 455
Fragments arranged by size:
2037 1443 455
BsaHI Gr'CG_yC
Cuts at: 634 703 1366 1614 2316 3812 634
Size: 69 663 248 702 1496 757
Fragments arranged by size:
1496 757 702 663 248 69
BsaWI w'CCGG_w
Cuts at: 117 135 1124 1645 2323 2597 2744 3155 117
Size: 18 989 521 678 274 147 411 897
Fragments arranged by size:
989 897 678 521 411 274 147 18
BseYI C'CCAG_C
Cuts at: 765 900 1360 2118 2695 3191 765
Size: 135 460 758 577 496 1509
Fragments arranged by size:
1509 758 577 496 460 135
BsiEI CG_ry'CG
Cuts at: 104 1477 1523 2731 104
Size: 1373 46 1208 1308
Fragments arranged by size:
1373 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 455 482 949 1727 1917 2709 455
Size: 27 467 778 190 792 1681
Fragments arranged by size:
1681 792 778 467 190 27
Bsp1286I G_dGCh'C
Cuts at: 455 482 949 1560 1653 1727 1917 1979 2709
Size: 27 467 611 93 74 190 62 730
Cuts at: 2709 455
Size: 1681
Fragments arranged by size:
1681 730 611 467 190 93 74 62
27
BspEI T'CCGG_A
Cuts at: 117 3155 117
Size: 3038 897
BspHI T'CATG_A
Cuts at: 3111 3111
Size: 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 602 672 985 1199 1501 1882 602
Size: 70 313 214 302 381 2655
Fragments arranged by size:
2655 381 313 302 214 70
BsrBI CCG'CTC
Cuts at: 50 2227 2281 3765 3930 50
Size: 2177 54 1484 165 55
Fragments arranged by size:
2177 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 798 911 1847 798
Size: 113 936 2886
Fragments arranged by size:
2886 936 113
BsrFI r'CCGG_y
Cuts at: 679 1933 2114 2323 679
Size: 1254 181 209 2291
Fragments arranged by size:
2291 1254 209 181
BssSI C'ACGA_G
Cuts at: 2206 2564 2206
Size: 358 3577
BstAPI GCAn_nnn'nTGC
Cuts at: 1312 1312
Size: 3935
BstBI TT'CG_AA
Cuts at: 2296 2296
Size: 3935
BstYI r'GATC_y
Cuts at: 1 539 885 960 1427 1450 1785 2031 3043
Size: 538 346 75 467 23 335 246 1012
Cuts at: 3043 3129 3141 3487 1
Size: 86 12 346 449
Fragments arranged by size:
1012 538 467 449 346 346 335 246
86 75 23 12
BtgI C'CryG_G
Cuts at: 494 843 494
Size: 349 3586
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1885 2066 2229 1885
Size: 181 163 3591
Fragments arranged by size:
3591 181 163
BtsI GCAGTG_nn'
Cuts at: 194 1178 194
Size: 984 2951
DraI TTT'AAA
Cuts at: 934 934
Size: 3935
DrdI GACnn_nn'nnGTC
Cuts at: 1641 2499 1641
Size: 858 3077
EaeI y'GGCC_r
Cuts at: 1520 1694 2085 2112 3457 1520
Size: 174 391 27 1345 1998
Fragments arranged by size:
1998 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1520 1520
Size: 3935
EarI CTCTTCn'nnn_
Cuts at: 908 1958 2168 908
Size: 1050 210 2675
Fragments arranged by size:
2675 1050 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2143 2453 2599 3745 2143
Size: 310 146 1146 2333
Fragments arranged by size:
2333 1146 310 146
EcoICRI GAG'CTC
Cuts at: 453 947 453
Size: 494 3441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 469 709 1760 2192 2939 3207 375
Size: 94 240 1051 432 747 268 1103
Fragments arranged by size:
1103 1051 747 432 268 240 94
EcoO109I rG'GnC_Cy
Cuts at: 591 783 1074 591
Size: 192 291 3452
Fragments arranged by size:
3452 291 192
EcoRI G'AATT_C
Cuts at: 298 559 939 298
Size: 261 380 3294
Fragments arranged by size:
3294 380 261
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1954 1954
Size: 3935
FspI TGC'GCA
Cuts at: 1716 1716
Size: 3935
HaeII r_GCGC'y
Cuts at: 126 1369 1617 2639 3618 126
Size: 1243 248 1022 979 443
Fragments arranged by size:
1243 1022 979 443 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 152 184 583 615 1777 1807 1809 1839 152
Size: 32 399 32 1162 30 2 30 2248
Fragments arranged by size:
2248 1162 399 32 32 30 30 2
HincII GTy'rAC
Cuts at: 974 974
Size: 3935
KasI G'GCGC_C
Cuts at: 1365 1613 1365
Size: 248 3687
KpnI G_GTAC'C
Cuts at: 955 955
Size: 3935
MluI A'CGCG_T
Cuts at: 3368 3368
Size: 3935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2606 2790 2606
Size: 184 3751
MscI TGG'CCA
Cuts at: 1696 1696
Size: 3935
MslI CAynn'nnrTG
Cuts at: 175 1924 2051 175
Size: 1749 127 2059
Fragments arranged by size:
2059 1749 127
MspA1I CmG'CkG
Cuts at: 198 447 755 1046 1360 1720 2733 2978 198
Size: 249 308 291 314 360 1013 245 1155
Fragments arranged by size:
1155 1013 360 314 308 291 249 245
NaeI GCC'GGC
Cuts at: 681 2116 681
Size: 1435 2500
NarI GG'CG_CC
Cuts at: 1366 1614 1366
Size: 248 3687
NcoI C'CATG_G
Cuts at: 494 494
Size: 3935
NgoMIV G'CCGG_C
Cuts at: 679 2114 679
Size: 1435 2500
NspI r_CATG'y
Cuts at: 166 988 1488 2395 166
Size: 822 500 907 1706
Fragments arranged by size:
1706 907 822 500
PciI A'CATG_T
Cuts at: 162 2391 162
Size: 2229 1706
PflMI CCAn_nnn'nTGG
Cuts at: 723 723
Size: 3935
PfoI T'CCnGG_A
Cuts at: 563 563
Size: 3935
PmeI GTTT'AAAC
Cuts at: 934 934
Size: 3935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 3903
PpuMI rG'GwC_Cy
Cuts at: 591 591
Size: 3935
PstI C_TGCA'G
Cuts at: 982 1667 982
Size: 685 3250
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3935
PvuII CAG'CTG
Cuts at: 198 755 1360 1720 198
Size: 557 605 360 2413
Fragments arranged by size:
2413 605 557 360
RsrII CG'GwC_CG
Cuts at: 2130 2130
Size: 3935
SacI G_AGCT'C
Cuts at: 455 949 455
Size: 494 3441
SalI G'TCGA_C
Cuts at: 972 972
Size: 3935
SapI GCTCTTCn'nnn_
Cuts at: 908 1958 2168 908
Size: 1050 210 2675
Fragments arranged by size:
2675 1050 210
SbfI CC_TGCA'GG
Cuts at: 982 982
Size: 3935
ScaI AGT'ACT
Cuts at: 3452 3452
Size: 3935
SfcI C'TryA_G
Cuts at: 33 978 1663 2656 2847 33
Size: 945 685 993 191 1121
Fragments arranged by size:
1121 993 945 685 191
SfoI GGC'GCC
Cuts at: 1367 1615 1367
Size: 248 3687
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
SmaI CCC'GGG
Cuts at: 957 957
Size: 3935
SmlI C'TyrA_G
Cuts at: 1090 2005 2497 2759 1090
Size: 915 492 262 2266
Fragments arranged by size:
2266 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3935
SphI G_CATG'C
Cuts at: 988 1488 988
Size: 500 3435
SspI AAT'ATT
Cuts at: 256 256
Size: 3935
StyI C'CwwG_G
Cuts at: 494 668 1069 1423 494
Size: 174 401 354 3006
Fragments arranged by size:
3006 401 354 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1518 1651 2110 2319 1518
Size: 133 459 209 3134
Fragments arranged by size:
3134 459 209 133
TatI w'GTAC_w
Cuts at: 749 3450 749
Size: 2701 1234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 175 207 718 750 3452 3484 175
Size: 32 511 32 2702 32 626
Fragments arranged by size:
2702 626 511 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 636 1732 636
Size: 1096 2839
XbaI T'CTAG_A
Cuts at: 966 966
Size: 3935
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1430 1430
Size: 3935
XmaI C'CCGG_G
Cuts at: 955 955
Size: 3935
ZraI GAC'GTC
Cuts at: 635 635
Size: 3935
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI