Restriction map for
pF1KE0402
(Circular) (Six-base) MAPSORT of: pF1KE0402.seq Check: 4549 from: 1 to: 4025
pF1KE0402 4025 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 860 1063 860
Size: 203 3822
Acc65I G'GTAC_C
Cuts at: 1041 1041
Size: 4025
AclI AA'CG_TT
Cuts at: 3865 3865
Size: 4025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 965 999 1850 2282 3029 3297 424
Size: 541 34 851 432 747 268 1152
Fragments arranged by size:
1152 851 747 541 432 268 34
AflIII A'CryG_T
Cuts at: 647 1377 2481 3458 647
Size: 730 1104 977 1214
Fragments arranged by size:
1214 1104 977 730
AgeI A'CCGG_T
Cuts at: 2413 2413
Size: 4025
AlwNI CAG_nnn'CTG
Cuts at: 331 1330 2897 331
Size: 999 1567 1459
Fragments arranged by size:
1567 1459 999
ApaLI G'TGCA_C
Cuts at: 700 2795 700
Size: 2095 1930
ApoI r'AATT_y
Cuts at: 567 910 1029 3378 3634 567
Size: 343 119 2349 256 958
Fragments arranged by size:
2349 958 343 256 119
AseI AT'TA_AT
Cuts at: 20 20
Size: 4025
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
AvaI C'yCGr_G
Cuts at: 1045 1045
Size: 4025
BamHI G'GATC_C
Cuts at: 872 1050 872
Size: 178 3847
BanI G'GyrC_C
Cuts at: 1041 1455 1703 1738 1041
Size: 414 248 35 3328
Fragments arranged by size:
3328 414 248 35
BanII G_rGCy'C
Cuts at: 282 1039 2069 282
Size: 757 1030 2238
Fragments arranged by size:
2238 1030 757
BbeI G_GCGC'C
Cuts at: 1459 1707 1459
Size: 248 3777
BbsI GAAGACnn'nnnn_
Cuts at: 169 169
Size: 4025
BciVI GTATCCnnnnn_n'
Cuts at: 1923 2684 4025 1923
Size: 761 1341 1923
Fragments arranged by size:
1923 1341 761
BclI T'GATC_A
Cuts at: 1545 1545
Size: 4025
BfrBI ATG'CAT
Cuts at: 451 451
Size: 4025
BglII A'GATC_T
Cuts at: 1540 3577 1540
Size: 2037 1988
BlpI GC'TnA_GC
Cuts at: 1137 1137
Size: 4025
Bme1580I G_kGCm'C
Cuts at: 704 1650 1743 2799 704
Size: 946 93 1056 1930
Fragments arranged by size:
1930 1056 946 93
BmgBI CAC'GTC
Cuts at: 469 469
Size: 4025
BmrI ACTGGGnnnn_n'
Cuts at: 1237 1318 1653 3797 1237
Size: 81 335 2144 1465
Fragments arranged by size:
2144 1465 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 681 2392 681
Size: 1711 2314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1013 1013
Size: 4025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1201 2080 2572 2870 1201
Size: 879 492 298 2356
Fragments arranged by size:
2356 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3729 3729
Size: 4025
BsaAI yAC'GTr
Cuts at: 650 1378 650
Size: 728 3297
BsaBI GATnn'nnATC
Cuts at: 6 1539 3576 6
Size: 1533 2037 455
Fragments arranged by size:
2037 1533 455
BsaHI Gr'CG_yC
Cuts at: 1456 1704 2406 3902 1456
Size: 248 702 1496 1579
Fragments arranged by size:
1579 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1214 1735 2413 2687 2834 3245 1214
Size: 521 678 274 147 411 1994
Fragments arranged by size:
1994 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 429 1450 2208 2785 3281 429
Size: 1021 758 577 496 1173
Fragments arranged by size:
1173 1021 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1567 1613 2821 104
Size: 1463 46 1208 1308
Fragments arranged by size:
1463 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 548 704 1039 1817 2007 2799 548
Size: 156 335 778 190 792 1774
Fragments arranged by size:
1774 792 778 335 190 156
BsmI GAATG_Cn'
Cuts at: 451 958 451
Size: 507 3518
Bsp1286I G_dGCh'C
Cuts at: 282 548 704 1039 1650 1743 1817 2007 2069
Size: 266 156 335 611 93 74 190 62
Cuts at: 2069 2799 282
Size: 730 1508
Fragments arranged by size:
1508 730 611 335 266 190 156 93
74 62
BspEI T'CCGG_A
Cuts at: 3245 3245
Size: 4025
BspHI T'CATG_A
Cuts at: 3201 3201
Size: 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1075 1289 1591 1972 1075
Size: 214 302 381 3128
Fragments arranged by size:
3128 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2317 2371 3855 4020 50
Size: 2267 54 1484 165 55
Fragments arranged by size:
2267 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1937 1937
Size: 4025
BsrFI r'CCGG_y
Cuts at: 2023 2204 2413 2023
Size: 181 209 3635
Fragments arranged by size:
3635 209 181
BssSI C'ACGA_G
Cuts at: 2296 2654 2296
Size: 358 3667
BstAPI GCAn_nnn'nTGC
Cuts at: 1402 1402
Size: 4025
BstBI TT'CG_AA
Cuts at: 2386 2386
Size: 4025
BstYI r'GATC_y
Cuts at: 1 872 1050 1517 1540 1875 2121 3133 3219
Size: 871 178 467 23 335 246 1012 86
Cuts at: 3219 3231 3577 1
Size: 12 346 449
Fragments arranged by size:
1012 871 467 449 346 335 246 178
86 23 12
BtgI C'CryG_G
Cuts at: 107 791 107
Size: 684 3341
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1975 2156 2319 1975
Size: 181 163 3681
Fragments arranged by size:
3681 181 163
BtsI GCAGTG_nn'
Cuts at: 129 1268 129
Size: 1139 2886
DraI TTT'AAA
Cuts at: 933 1024 933
Size: 91 3934
DrdI GACnn_nn'nnGTC
Cuts at: 856 1731 2589 856
Size: 875 858 2292
Fragments arranged by size:
2292 875 858
EaeI y'GGCC_r
Cuts at: 1610 1784 2175 2202 3547 1610
Size: 174 391 27 1345 2088
Fragments arranged by size:
2088 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1610 1610
Size: 4025
EarI CTCTTCn'nnn_
Cuts at: 131 263 355 758 2048 2258 131
Size: 132 92 403 1290 210 1898
Fragments arranged by size:
1898 1290 403 210 132 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2233 2543 2689 3835 2233
Size: 310 146 1146 2423
Fragments arranged by size:
2423 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1037 1037
Size: 4025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 965 999 1013 1850 2282 3029 3297 424
Size: 541 34 14 837 432 747 268 1152
Fragments arranged by size:
1152 837 747 541 432 268 34 14
EcoNI CCTnn'n_nnAGG
Cuts at: 287 287
Size: 4025
EcoO109I rG'GnC_Cy
Cuts at: 293 462 1164 293
Size: 169 702 3154
Fragments arranged by size:
3154 702 169
EcoRI G'AATT_C
Cuts at: 567 910 1029 567
Size: 343 119 3563
Fragments arranged by size:
3563 343 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 366 2044 366
Size: 1678 2347
FspI TGC'GCA
Cuts at: 1806 1806
Size: 4025
HaeII r_GCGC'y
Cuts at: 1459 1707 2729 3708 1459
Size: 248 1022 979 1776
Fragments arranged by size:
1776 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 1867 1897 1899 1929 217
Size: 32 1618 30 2 30 2313
Fragments arranged by size:
2313 1618 32 30 30 2
HincII GTy'rAC
Cuts at: 176 560 1064 176
Size: 384 504 3137
Fragments arranged by size:
3137 504 384
HpaI GTT'AAC
Cuts at: 560 560
Size: 4025
KasI G'GCGC_C
Cuts at: 1455 1703 1455
Size: 248 3777
KpnI G_GTAC'C
Cuts at: 1045 1045
Size: 4025
MluI A'CGCG_T
Cuts at: 3458 3458
Size: 4025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 850 2696 2880 850
Size: 1846 184 1995
Fragments arranged by size:
1995 1846 184
MscI TGG'CCA
Cuts at: 1786 1786
Size: 4025
MslI CAynn'nnrTG
Cuts at: 736 829 2014 2141 736
Size: 93 1185 127 2620
Fragments arranged by size:
2620 1185 127 93
MspA1I CmG'CkG
Cuts at: 433 1136 1450 1810 2823 3068 433
Size: 703 314 360 1013 245 1390
Fragments arranged by size:
1390 1013 703 360 314 245
NaeI GCC'GGC
Cuts at: 2206 2206
Size: 4025
NarI GG'CG_CC
Cuts at: 1456 1704 1456
Size: 248 3777
NcoI C'CATG_G
Cuts at: 107 107
Size: 4025
NdeI CA'TA_TG
Cuts at: 596 596
Size: 4025
NgoMIV G'CCGG_C
Cuts at: 2204 2204
Size: 4025
NsiI A_TGCA'T
Cuts at: 453 453
Size: 4025
NspI r_CATG'y
Cuts at: 1078 1578 2485 1078
Size: 500 907 2618
Fragments arranged by size:
2618 907 500
PciI A'CATG_T
Cuts at: 2481 2481
Size: 4025
PmeI GTTT'AAAC
Cuts at: 1024 1024
Size: 4025
PmlI CAC'GTG
Cuts at: 650 650
Size: 4025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3993
PpuMI rG'GwC_Cy
Cuts at: 462 462
Size: 4025
PstI C_TGCA'G
Cuts at: 309 1072 1757 309
Size: 763 685 2577
Fragments arranged by size:
2577 763 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4025
PvuII CAG'CTG
Cuts at: 433 1450 1810 433
Size: 1017 360 2648
Fragments arranged by size:
2648 1017 360
RsrII CG'GwC_CG
Cuts at: 2220 2220
Size: 4025
SacI G_AGCT'C
Cuts at: 1039 1039
Size: 4025
SalI G'TCGA_C
Cuts at: 1062 1062
Size: 4025
SapI GCTCTTCn'nnn_
Cuts at: 263 758 2048 2258 263
Size: 495 1290 210 2030
Fragments arranged by size:
2030 1290 495 210
SbfI CC_TGCA'GG
Cuts at: 1072 1072
Size: 4025
ScaI AGT'ACT
Cuts at: 3542 3542
Size: 4025
SfcI C'TryA_G
Cuts at: 33 305 1068 1753 2746 2937 33
Size: 272 763 685 993 191 1121
Fragments arranged by size:
1121 993 763 685 272 191
SfoI GGC'GCC
Cuts at: 1457 1705 1457
Size: 248 3777
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
SmaI CCC'GGG
Cuts at: 1047 1047
Size: 4025
SmlI C'TyrA_G
Cuts at: 1180 2095 2587 2849 1180
Size: 915 492 262 2356
Fragments arranged by size:
2356 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4025
SphI G_CATG'C
Cuts at: 1078 1578 1078
Size: 500 3525
StyI C'CwwG_G
Cuts at: 107 492 1159 1513 107
Size: 385 667 354 2619
Fragments arranged by size:
2619 667 385 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1608 1741 2200 2409 1608
Size: 133 459 209 3224
Fragments arranged by size:
3224 459 209 133
TatI w'GTAC_w
Cuts at: 356 821 3540 356
Size: 465 2719 841
Fragments arranged by size:
2719 841 465
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3542 3574 3542
Size: 32 3993
Tth111I GACn'n_nGTC
Cuts at: 1822 1822
Size: 4025
XbaI T'CTAG_A
Cuts at: 846 1056 846
Size: 210 3815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1520 1520
Size: 4025
XmaI C'CCGG_G
Cuts at: 1045 1045
Size: 4025
XmnI GAAnn'nnTTC
Cuts at: 409 958 409
Size: 549 3476
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII MfeI NheI NotI NruI PacI
PasI PflMI PfoI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI