Restriction map for
pF1KE0394
(Circular) (Six-base) MAPSORT of: pF1KE0394.seq Check: 5206 from: 1 to: 6473
pF1KE0394 6473 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 3511 3511
Size: 6473
Acc65I G'GTAC_C
Cuts at: 1719 1836 3489 1719
Size: 117 1653 4703
Fragments arranged by size:
4703 1653 117
AclI AA'CG_TT
Cuts at: 6313 6313
Size: 6473
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2092 2315 4298 4730 5477 5745 2092
Size: 223 1983 432 747 268 2820
Fragments arranged by size:
2820 1983 747 432 268 223
AflIII A'CryG_T
Cuts at: 393 1525 3064 3167 3825 4929 5906 393
Size: 1132 1539 103 658 1104 977 960
Fragments arranged by size:
1539 1132 1104 977 960 658 103
AgeI A'CCGG_T
Cuts at: 4861 4861
Size: 6473
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2267 2267
Size: 6473
AlwNI CAG_nnn'CTG
Cuts at: 2403 3045 3778 5345 2403
Size: 642 733 1567 3531
Fragments arranged by size:
3531 1567 733 642
ApaI G_GGCC'C
Cuts at: 1688 2606 1688
Size: 918 5555
ApaLI G'TGCA_C
Cuts at: 1242 5243 1242
Size: 4001 2472
ApoI r'AATT_y
Cuts at: 574 708 1670 2504 2512 2809 3477 5826 6082
Size: 134 962 834 8 297 668 2349 256
Cuts at: 6082 574
Size: 965
Fragments arranged by size:
2349 965 962 834 668 297 256 134
8
AseI AT'TA_AT
Cuts at: 20 542 626 20
Size: 522 84 5867
Fragments arranged by size:
5867 522 84
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6473
AvaI C'yCGr_G
Cuts at: 1094 1634 3493 1094
Size: 540 1859 4074
Fragments arranged by size:
4074 1859 540
BamHI G'GATC_C
Cuts at: 3498 3498
Size: 6473
BanI G'GyrC_C
Cuts at: 1485 1719 1836 3306 3489 3903 4151 4186 1485
Size: 234 117 1470 183 414 248 35 3772
Fragments arranged by size:
3772 1470 414 248 234 183 117 35
BanII G_rGCy'C
Cuts at: 1095 1499 1688 2606 3487 4517 1095
Size: 404 189 918 881 1030 3051
Fragments arranged by size:
3051 1030 918 881 404 189
BbeI G_GCGC'C
Cuts at: 3907 4155 3907
Size: 248 6225
BbsI GAAGACnn'nnnn_
Cuts at: 2497 2497
Size: 6473
BbvCI CC'TCA_GC
Cuts at: 2000 3038 2000
Size: 1038 5435
BciVI GTATCCnnnnn_n'
Cuts at: 4371 5132 6473 4371
Size: 761 1341 4371
Fragments arranged by size:
4371 1341 761
BclI T'GATC_A
Cuts at: 368 957 3993 368
Size: 589 3036 2848
Fragments arranged by size:
3036 2848 589
BfrBI ATG'CAT
Cuts at: 446 446
Size: 6473
BglI GCCn_nnn'nGGC
Cuts at: 260 1099 260
Size: 839 5634
BglII A'GATC_T
Cuts at: 3988 6025 3988
Size: 2037 4436
BlpI GC'TnA_GC
Cuts at: 3585 3585
Size: 6473
Bme1580I G_kGCm'C
Cuts at: 1246 1688 2606 4098 4191 5247 1246
Size: 442 918 1492 93 1056 2472
Fragments arranged by size:
2472 1492 1056 918 442 93
BmgBI CAC'GTC
Cuts at: 1432 1432
Size: 6473
BmrI ACTGGGnnnn_n'
Cuts at: 3284 3685 3766 4101 6245 3284
Size: 401 81 335 2144 3512
Fragments arranged by size:
3512 2144 401 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4840 4840
Size: 6473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 231 371 759 770 1316 1579 1826 2548
Size: 104 140 388 11 546 263 247 722
Cuts at: 2548 127
Size: 4052
Fragments arranged by size:
4052 722 546 388 263 247 140 104
11
Bpu10I CC'TnA_GC
Cuts at: 1373 2000 3038 1373
Size: 627 1038 4808
Fragments arranged by size:
4808 1038 627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 1268 1394 1723 3649 4528 5020 5318 589
Size: 679 126 329 1926 879 492 298 1744
Fragments arranged by size:
1926 1744 879 679 492 329 298 126
BsaI GGTCTCn'nnnn_
Cuts at: 6177 6177
Size: 6473
BsaAI yAC'GTr
Cuts at: 396 3826 396
Size: 3430 3043
BsaBI GATnn'nnATC
Cuts at: 6 2152 3987 6024 6
Size: 2146 1835 2037 455
Fragments arranged by size:
2146 2037 1835 455
BsaHI Gr'CG_yC
Cuts at: 3904 4152 4854 6350 3904
Size: 248 702 1496 4027
Fragments arranged by size:
4027 1496 702 248
BsaWI w'CCGG_w
Cuts at: 528 3662 4183 4861 5135 5282 5693 528
Size: 3134 521 678 274 147 411 1308
Fragments arranged by size:
3134 1308 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 713 743 1577 1607 2063 2093 713
Size: 30 834 30 456 30 5093
Fragments arranged by size:
5093 834 456 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 1555 1846 2773 3334 373
Size: 1182 291 927 561 3512
Fragments arranged by size:
3512 1182 927 561 291
BseYI C'CCAG_C
Cuts at: 3898 4656 5233 5729 3898
Size: 758 577 496 4642
Fragments arranged by size:
4642 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3099 4015 4061 5269 104
Size: 2995 916 46 1208 1308
Fragments arranged by size:
2995 1308 1208 916 46
BsiHKAI G_wGCw'C
Cuts at: 116 745 848 1246 1595 1785 2078 3487 4265
Size: 629 103 398 349 190 293 1409 778
Cuts at: 4265 4455 5247 116
Size: 190 792 1342
Fragments arranged by size:
1409 1342 792 778 629 398 349 293
190 190 103
BsmBI CGTCTCn'nnnn_
Cuts at: 1422 1422
Size: 6473
Bsp1286I G_dGCh'C
Cuts at: 116 745 848 1095 1246 1499 1595 1688 1785
Size: 629 103 247 151 253 96 93 97
Cuts at: 1785 2078 2606 3487 4098 4191 4265 4455 4517
Size: 293 528 881 611 93 74 190 62
Cuts at: 4517 5247 116
Size: 730 1342
Fragments arranged by size:
1342 881 730 629 611 528 293 253
247 190 151 103 97 96 93 93
74 62
BspEI T'CCGG_A
Cuts at: 5693 5693
Size: 6473
BspHI T'CATG_A
Cuts at: 947 5649 947
Size: 4702 1771
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3523 3737 4039 4420 3523
Size: 214 302 381 5576
Fragments arranged by size:
5576 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4765 4819 6303 6468 50
Size: 4715 54 1484 165 55
Fragments arranged by size:
4715 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 240 4385 240
Size: 4145 2328
BsrFI r'CCGG_y
Cuts at: 4471 4652 4861 4471
Size: 181 209 6083
Fragments arranged by size:
6083 209 181
BsrGI T'GTAC_A
Cuts at: 987 3059 987
Size: 2072 4401
BssSI C'ACGA_G
Cuts at: 4744 5102 4744
Size: 358 6115
BstAPI GCAn_nnn'nTGC
Cuts at: 1369 1780 3850 1369
Size: 411 2070 3992
Fragments arranged by size:
3992 2070 411
BstBI TT'CG_AA
Cuts at: 1943 4834 1943
Size: 2891 3582
BstYI r'GATC_y
Cuts at: 1 299 3498 3965 3988 4323 4569 5581 5667
Size: 298 3199 467 23 335 246 1012 86
Cuts at: 5667 5679 6025 1
Size: 12 346 449
Fragments arranged by size:
3199 1012 467 449 346 335 298 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 195 107
Size: 88 6385
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2127 4423 4604 4767 2127
Size: 2296 181 163 3833
Fragments arranged by size:
3833 2296 181 163
BtsI GCAGTG_nn'
Cuts at: 152 1325 3022 3716 152
Size: 1173 1697 694 2909
Fragments arranged by size:
2909 1697 1173 694
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1938 1973 1938
Size: 35 6438
DraI TTT'AAA
Cuts at: 3472 3472
Size: 6473
DrdI GACnn_nn'nnGTC
Cuts at: 4179 5037 4179
Size: 858 5615
EaeI y'GGCC_r
Cuts at: 3077 3248 4058 4232 4623 4650 5995 3077
Size: 171 810 174 391 27 1345 3555
Fragments arranged by size:
3555 1345 810 391 174 171 27
EagI C'GGCC_G
Cuts at: 4058 4058
Size: 6473
EarI CTCTTCn'nnn_
Cuts at: 1653 2920 3226 4496 4706 1653
Size: 1267 306 1270 210 3420
Fragments arranged by size:
3420 1270 1267 306 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4681 4991 5137 6283 4681
Size: 310 146 1146 4871
Fragments arranged by size:
4871 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3485 3485
Size: 6473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 231 371 759 770 1316 1579 1826 2092
Size: 104 140 388 11 546 263 247 266
Cuts at: 2092 2315 2548 4298 4730 5477 5745 127
Size: 223 233 1750 432 747 268 855
Fragments arranged by size:
1750 855 747 546 432 388 268 266
263 247 233 223 140 104 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2978 2978
Size: 6473
EcoO109I rG'GnC_Cy
Cuts at: 1684 2799 3612 1684
Size: 1115 813 4545
Fragments arranged by size:
4545 1115 813
EcoRI G'AATT_C
Cuts at: 2512 3477 2512
Size: 965 5508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 529 2122 4492 529
Size: 1593 2370 2510
Fragments arranged by size:
2510 2370 1593
FspI TGC'GCA
Cuts at: 4254 4254
Size: 6473
HaeII r_GCGC'y
Cuts at: 3045 3907 4155 5177 6156 3045
Size: 862 248 1022 979 3362
Fragments arranged by size:
3362 1022 979 862 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 369 391 401 423 676 708 2177 2209 2893
Size: 22 10 22 253 32 1469 32 684
Cuts at: 2893 2925 3114 3146 4315 4345 4347 4377 369
Size: 32 189 32 1169 30 2 30 2465
Fragments arranged by size:
2465 1469 1169 684 253 189 32 32
32 32 30 30 22 22 10 2
HincII GTy'rAC
Cuts at: 192 2214 2310 2444 2568 3512 192
Size: 2022 96 134 124 944 3153
Fragments arranged by size:
3153 2022 944 134 124 96
HindIII A'AGCT_T
Cuts at: 506 1462 2669 3204 506
Size: 956 1207 535 3775
Fragments arranged by size:
3775 1207 956 535
KasI G'GCGC_C
Cuts at: 3903 4151 3903
Size: 248 6225
KpnI G_GTAC'C
Cuts at: 1723 1840 3493 1723
Size: 117 1653 4703
Fragments arranged by size:
4703 1653 117
MluI A'CGCG_T
Cuts at: 5906 5906
Size: 6473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2751 5144 5328 2751
Size: 2393 184 3896
Fragments arranged by size:
3896 2393 184
MscI TGG'CCA
Cuts at: 3079 3250 4234 3079
Size: 171 984 5318
Fragments arranged by size:
5318 984 171
MslI CAynn'nnrTG
Cuts at: 451 1988 2156 4462 4589 451
Size: 1537 168 2306 127 2335
Fragments arranged by size:
2335 2306 1537 168 127
MspA1I CmG'CkG
Cuts at: 424 1219 2662 3584 3898 4258 5271 5516 424
Size: 795 1443 922 314 360 1013 245 1381
Fragments arranged by size:
1443 1381 1013 922 795 360 314 245
NaeI GCC'GGC
Cuts at: 4654 4654
Size: 6473
NarI GG'CG_CC
Cuts at: 3904 4152 3904
Size: 248 6225
NcoI C'CATG_G
Cuts at: 107 195 107
Size: 88 6385
NgoMIV G'CCGG_C
Cuts at: 4652 4652
Size: 6473
NsiI A_TGCA'T
Cuts at: 448 448
Size: 6473
NspI r_CATG'y
Cuts at: 1456 1529 3014 3068 3171 3436 3526 4026 4933
Size: 73 1485 54 103 265 90 500 907
Cuts at: 4933 1456
Size: 2996
Fragments arranged by size:
2996 1485 907 500 265 103 90 73
54
PciI A'CATG_T
Cuts at: 1525 3064 3167 4929 1525
Size: 1539 103 1762 3069
Fragments arranged by size:
3069 1762 1539 103
PflMI CCAn_nnn'nTGG
Cuts at: 1747 2403 1747
Size: 656 5817
PfoI T'CCnGG_A
Cuts at: 387 998 1595 1842 387
Size: 611 597 247 5018
Fragments arranged by size:
5018 611 597 247
PmeI GTTT'AAAC
Cuts at: 3472 3472
Size: 6473
PmlI CAC'GTG
Cuts at: 396 396
Size: 6473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5612 5644 5612
Size: 32 6441
PpuMI rG'GwC_Cy
Cuts at: 2799 2799
Size: 6473
PspOMI G'GGCC_C
Cuts at: 1684 2602 1684
Size: 918 5555
PstI C_TGCA'G
Cuts at: 3520 4205 3520
Size: 685 5788
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6473
PvuII CAG'CTG
Cuts at: 1219 2662 3898 4258 1219
Size: 1443 1236 360 3434
Fragments arranged by size:
3434 1443 1236 360
RsrII CG'GwC_CG
Cuts at: 4668 4668
Size: 6473
SacI G_AGCT'C
Cuts at: 3487 3487
Size: 6473
SalI G'TCGA_C
Cuts at: 3510 3510
Size: 6473
SapI GCTCTTCn'nnn_
Cuts at: 4496 4706 4496
Size: 210 6263
SbfI CC_TGCA'GG
Cuts at: 3520 3520
Size: 6473
ScaI AGT'ACT
Cuts at: 5990 5990
Size: 6473
SfcI C'TryA_G
Cuts at: 33 861 3516 4201 5194 5385 33
Size: 828 2655 685 993 191 1121
Fragments arranged by size:
2655 1121 993 828 685 191
SfoI GGC'GCC
Cuts at: 3905 4153 3905
Size: 248 6225
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6473
SmaI CCC'GGG
Cuts at: 1096 3495 1096
Size: 2399 4074
SmlI C'TyrA_G
Cuts at: 604 1283 1409 1702 3628 4543 5035 5297 604
Size: 679 126 293 1926 915 492 262 1780
Fragments arranged by size:
1926 1780 915 679 492 293 262 126
SpeI A'CTAG_T
Cuts at: 67 2613 3261 67
Size: 2546 648 3279
Fragments arranged by size:
3279 2546 648
SphI G_CATG'C
Cuts at: 1456 3436 3526 4026 1456
Size: 1980 90 500 3903
Fragments arranged by size:
3903 1980 500 90
SspI AAT'ATT
Cuts at: 171 612 171
Size: 441 6032
StuI AGG'CCT
Cuts at: 592 1378 1401 1868 2917 592
Size: 786 23 467 1049 4148
Fragments arranged by size:
4148 1049 786 467 23
StyI C'CwwG_G
Cuts at: 107 195 1396 1490 3607 3961 107
Size: 88 1201 94 2117 354 2619
Fragments arranged by size:
2619 2117 1201 354 94 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 815 1516 2410 2761 3085 3294 4056 4189 4648
Size: 701 894 351 324 209 762 133 459
Cuts at: 4648 4857 815
Size: 209 2431
Fragments arranged by size:
2431 894 762 701 459 351 324 209
209 133
TatI w'GTAC_w
Cuts at: 987 3059 3374 5988 987
Size: 2072 315 2614 1472
Fragments arranged by size:
2614 2072 1472 315
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 159 191 788 820 5990 6022 159
Size: 32 597 32 5170 32 610
Fragments arranged by size:
5170 610 597 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2218 4270 2218
Size: 2052 4421
XbaI T'CTAG_A
Cuts at: 1827 3504 1827
Size: 1677 4796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3968 3968
Size: 6473
XmaI C'CCGG_G
Cuts at: 1094 3493 1094
Size: 2399 4074
XmnI GAAnn'nnTTC
Cuts at: 2633 2633
Size: 6473
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BmtI BsgI BsiWI BsmI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI