Restriction map for
pF1KE0382
(Circular) (Six-base) MAPSORT of: pF1KE0382.seq Check: 1225 from: 1 to: 5243
pF1KE0382 5243 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AatII G_ACGT'C
Cuts at: 557 557
Size: 5243
AccI GT'mk_AC
Cuts at: 143 557 2281 143
Size: 414 1724 3105
Fragments arranged by size:
3105 1724 414
Acc65I G'GTAC_C
Cuts at: 1729 2259 1729
Size: 530 4713
AclI AA'CG_TT
Cuts at: 5083 5083
Size: 5243
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 3068 3500 4247 4515 231
Size: 2837 432 747 268 959
Fragments arranged by size:
2837 959 747 432 268
AfeI AGC'GCT
Cuts at: 378 378
Size: 5243
AflIII A'CryG_T
Cuts at: 2595 3699 4676 2595
Size: 1104 977 3162
Fragments arranged by size:
3162 1104 977
AgeI A'CCGG_T
Cuts at: 3631 3631
Size: 5243
AleI CACnn'nnGTG
Cuts at: 286 2035 286
Size: 1749 3494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2053 2053
Size: 5243
AlwNI CAG_nnn'CTG
Cuts at: 1185 1623 1632 1713 2548 4115 1185
Size: 438 9 81 835 1567 2313
Fragments arranged by size:
2313 1567 835 438 81 9
ApaI G_GGCC'C
Cuts at: 1513 1679 1513
Size: 166 5077
ApaLI G'TGCA_C
Cuts at: 966 1703 4013 966
Size: 737 2310 2196
Fragments arranged by size:
2310 2196 737
ApoI r'AATT_y
Cuts at: 2114 2247 4596 4852 2114
Size: 133 2349 256 2505
Fragments arranged by size:
2505 2349 256 133
AseI AT'TA_AT
Cuts at: 20 20
Size: 5243
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5243
AvaI C'yCGr_G
Cuts at: 329 350 361 1606 1670 2263 329
Size: 21 11 1245 64 593 3309
Fragments arranged by size:
3309 1245 593 64 21 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1594 1627 1594
Size: 33 5210
BamHI G'GATC_C
Cuts at: 1459 2268 1459
Size: 809 4434
BanI G'GyrC_C
Cuts at: 1650 1729 2259 2673 2921 2956 1650
Size: 79 530 414 248 35 3937
Fragments arranged by size:
3937 530 414 248 79 35
BanII G_rGCy'C
Cuts at: 157 1513 1679 2235 2257 3287 157
Size: 1356 166 556 22 1030 2113
Fragments arranged by size:
2113 1356 1030 556 166 22
BbeI G_GCGC'C
Cuts at: 2677 2925 2677
Size: 248 4995
BbsI GAAGACnn'nnnn_
Cuts at: 942 1473 942
Size: 531 4712
BbvCI CC'TCA_GC
Cuts at: 1961 1961
Size: 5243
BciVI GTATCCnnnnn_n'
Cuts at: 1159 1595 1651 3141 3902 5243 1159
Size: 436 56 1490 761 1341 1159
Fragments arranged by size:
1490 1341 1159 761 436 56
BclI T'GATC_A
Cuts at: 794 2763 794
Size: 1969 3274
BglII A'GATC_T
Cuts at: 470 1152 2758 4795 470
Size: 682 1606 2037 918
Fragments arranged by size:
2037 1606 918 682
BlpI GC'TnA_GC
Cuts at: 241 2355 241
Size: 2114 3129
Bme1580I G_kGCm'C
Cuts at: 970 1513 1679 1707 2868 2961 4017 970
Size: 543 166 28 1161 93 1056 2196
Fragments arranged by size:
2196 1161 1056 543 166 93 28
BmgBI CAC'GTC
Cuts at: 762 1122 762
Size: 360 4883
BmrI ACTGGGnnnn_n'
Cuts at: 843 2455 2536 2871 5015 843
Size: 1612 81 335 2144 1071
Fragments arranged by size:
2144 1612 1071 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 864 3610 864
Size: 2746 2497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 393 889 1567 1767 1942 2143 141
Size: 252 496 678 200 175 201 3241
Fragments arranged by size:
3241 678 496 252 201 200 175
Bpu10I CC'TnA_GC
Cuts at: 984 1961 984
Size: 977 4266
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 2419 3298 3790 4088 154
Size: 2265 879 492 298 1309
Fragments arranged by size:
2265 1309 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 289 4947 289
Size: 4658 585
BsaAI yAC'GTr
Cuts at: 1058 2596 1058
Size: 1538 3705
BsaBI GATnn'nnATC
Cuts at: 6 2757 4794 6
Size: 2751 2037 455
Fragments arranged by size:
2751 2037 455
BsaHI Gr'CG_yC
Cuts at: 194 554 2674 2922 3624 5120 194
Size: 360 2120 248 702 1496 317
Fragments arranged by size:
2120 1496 702 360 317 248
BsaWI w'CCGG_w
Cuts at: 298 1145 2432 2953 3631 3905 4052 4463 298
Size: 847 1287 521 678 274 147 411 1078
Fragments arranged by size:
1287 1078 847 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1777 1807 2093 2123 1777
Size: 30 286 30 4897
Fragments arranged by size:
4897 286 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1483 1792 1483
Size: 309 4934
BseYI C'CCAG_C
Cuts at: 321 507 749 944 1199 1506 2227 2668 3426
Size: 186 242 195 255 307 721 441 758
Cuts at: 3426 4003 4499 321
Size: 577 496 1065
Fragments arranged by size:
1065 758 721 577 496 441 307 255
242 195 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 795 2013 795
Size: 1218 4025
BsiEI CG_ry'CG
Cuts at: 104 2785 2831 4039 104
Size: 2681 46 1208 1308
Fragments arranged by size:
2681 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 157 176 970 1707 2257 3035 3225 4017 157
Size: 19 794 737 550 778 190 792 1383
Fragments arranged by size:
1383 794 792 778 737 550 190 19
Bsp1286I G_dGCh'C
Cuts at: 157 176 970 1513 1679 1707 2235 2257 2868
Size: 19 794 543 166 28 528 22 611
Cuts at: 2868 2961 3035 3225 3287 4017 157
Size: 93 74 190 62 730 1383
Fragments arranged by size:
1383 794 730 611 543 528 190 166
93 74 62 28 22 19
BspEI T'CCGG_A
Cuts at: 1145 4463 1145
Size: 3318 1925
BspHI T'CATG_A
Cuts at: 624 1484 4419 624
Size: 860 2935 1448
Fragments arranged by size:
2935 1448 860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 155 569 1170 1631 2293 2507 2809 3190 155
Size: 414 601 461 662 214 302 381 2208
Fragments arranged by size:
2208 662 601 461 414 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1800 3535 3589 5073 5238 50
Size: 1750 1735 54 1484 165 55
Fragments arranged by size:
1750 1735 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3155 3155
Size: 5243
BsrFI r'CCGG_y
Cuts at: 3241 3422 3631 3241
Size: 181 209 4853
Fragments arranged by size:
4853 209 181
BssSI C'ACGA_G
Cuts at: 3514 3872 3514
Size: 358 4885
BstAPI GCAn_nnn'nTGC
Cuts at: 1632 1758 2620 1632
Size: 126 862 4255
Fragments arranged by size:
4255 862 126
BstBI TT'CG_AA
Cuts at: 3604 3604
Size: 5243
BstXI CCAn_nnnn'nTGG
Cuts at: 1686 1686
Size: 5243
BstYI r'GATC_y
Cuts at: 1 470 698 1152 1459 1539 2268 2735 2758
Size: 469 228 454 307 80 729 467 23
Cuts at: 2758 3093 3339 4351 4437 4449 4795 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 729 469 467 454 449 346 335
307 246 228 86 80 23 12
Bsu36I CC'TnA_GG
Cuts at: 1466 1466
Size: 5243
BtgI C'CryG_G
Cuts at: 107 216 107
Size: 109 5134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1851 3193 3374 3537 1851
Size: 1342 181 163 3557
Fragments arranged by size:
3557 1342 181 163
BtsI GCAGTG_nn'
Cuts at: 888 907 1218 2486 888
Size: 19 311 1268 3645
Fragments arranged by size:
3645 1268 311 19
ClaI AT'CG_AT
Cuts at: 1052 1052
Size: 5243
DraI TTT'AAA
Cuts at: 2242 2242
Size: 5243
DraIII CAC_nnn'GTG
Cuts at: 495 1032 1758 495
Size: 537 726 3980
Fragments arranged by size:
3980 726 537
DrdI GACnn_nn'nnGTC
Cuts at: 1890 2215 2949 3807 1890
Size: 325 734 858 3326
Fragments arranged by size:
3326 858 734 325
EaeI y'GGCC_r
Cuts at: 219 447 734 887 1341 1595 1795 2096 2828
Size: 228 287 153 454 254 200 301 732
Cuts at: 2828 3002 3393 3420 4765 219
Size: 174 391 27 1345 697
Fragments arranged by size:
1345 732 697 454 391 301 287 254
228 200 174 153 27
EagI C'GGCC_G
Cuts at: 2828 2828
Size: 5243
EarI CTCTTCn'nnn_
Cuts at: 665 1874 3266 3476 665
Size: 1209 1392 210 2432
Fragments arranged by size:
2432 1392 1209 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3451 3761 3907 5053 3451
Size: 310 146 1146 3641
Fragments arranged by size:
3641 1146 310 146
EcoICRI GAG'CTC
Cuts at: 155 2255 155
Size: 2100 3143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 231 393 889 1567 1767 1942 2143 3068
Size: 90 162 496 678 200 175 201 925
Cuts at: 3068 3500 4247 4515 141
Size: 432 747 268 869
Fragments arranged by size:
925 869 747 678 496 432 268 201
200 175 162 90
EcoNI CCTnn'n_nnAGG
Cuts at: 1746 1746
Size: 5243
EcoO109I rG'GnC_Cy
Cuts at: 326 1510 1620 1675 1710 2079 2382 326
Size: 1184 110 55 35 369 303 3187
Fragments arranged by size:
3187 1184 369 303 110 55 35
EcoRI G'AATT_C
Cuts at: 2247 2247
Size: 5243
EcoRV GAT'ATC
Cuts at: 1914 1914
Size: 5243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 436 1541 2229 3262 436
Size: 1105 688 1033 2417
Fragments arranged by size:
2417 1105 1033 688
FspI TGC'GCA
Cuts at: 3024 3024
Size: 5243
HaeII r_GCGC'y
Cuts at: 380 1425 1978 2677 2925 3947 4926 380
Size: 1045 553 699 248 1022 979 697
Fragments arranged by size:
1045 1022 979 699 697 553 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 388 420 702 734 1069 1101 1973 2005 2093
Size: 32 282 32 335 32 872 32 88
Cuts at: 2093 2125 3085 3115 3117 3147 388
Size: 32 960 30 2 30 2484
Fragments arranged by size:
2484 960 872 335 282 88 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 144 1088 2282 144
Size: 944 1194 3105
Fragments arranged by size:
3105 1194 944
KasI G'GCGC_C
Cuts at: 2673 2921 2673
Size: 248 4995
KpnI G_GTAC'C
Cuts at: 1733 2263 1733
Size: 530 4713
MluI A'CGCG_T
Cuts at: 4676 4676
Size: 5243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 425 1018 3914 4098 425
Size: 593 2896 184 1570
Fragments arranged by size:
2896 1570 593 184
MscI TGG'CCA
Cuts at: 449 889 1343 1597 3004 449
Size: 440 454 254 1407 2688
Fragments arranged by size:
2688 1407 454 440 254
MslI CAynn'nnrTG
Cuts at: 286 973 1402 1724 1855 2008 2035 3232 3359
Size: 687 429 322 131 153 27 1197 127
Cuts at: 3359 286
Size: 2170
Fragments arranged by size:
2170 1197 687 429 322 153 131 127
27
MspA1I CmG'CkG
Cuts at: 218 507 753 1581 1629 2027 2354 2668 3028
Size: 289 246 828 48 398 327 314 360
Cuts at: 3028 4041 4286 218
Size: 1013 245 1175
Fragments arranged by size:
1175 1013 828 398 360 327 314 289
246 245 48
NaeI GCC'GGC
Cuts at: 3424 3424
Size: 5243
NarI GG'CG_CC
Cuts at: 2674 2922 2674
Size: 248 4995
NcoI C'CATG_G
Cuts at: 107 107
Size: 5243
NgoMIV G'CCGG_C
Cuts at: 3422 3422
Size: 5243
NspI r_CATG'y
Cuts at: 2296 2796 3703 2296
Size: 500 907 3836
Fragments arranged by size:
3836 907 500
PciI A'CATG_T
Cuts at: 3699 3699
Size: 5243
PfoI T'CCnGG_A
Cuts at: 157 1535 1544 157
Size: 1378 9 3856
Fragments arranged by size:
3856 1378 9
PmeI GTTT'AAAC
Cuts at: 2242 2242
Size: 5243
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 4382
Size: 32 5211
PpuMI rG'GwC_Cy
Cuts at: 326 1620 2079 326
Size: 1294 459 3490
Fragments arranged by size:
3490 1294 459
PspOMI G'GGCC_C
Cuts at: 1509 1675 1509
Size: 166 5077
PstI C_TGCA'G
Cuts at: 152 584 827 1586 1628 2290 2975 152
Size: 432 243 759 42 662 685 2420
Fragments arranged by size:
2420 759 685 662 432 243 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5243
PvuII CAG'CTG
Cuts at: 1581 1629 2027 2668 3028 1581
Size: 48 398 641 360 3796
Fragments arranged by size:
3796 641 398 360 48
RsrII CG'GwC_CG
Cuts at: 3438 3438
Size: 5243
SacI G_AGCT'C
Cuts at: 157 2257 157
Size: 2100 3143
SacII CC_GC'GG
Cuts at: 219 219
Size: 5243
SalI G'TCGA_C
Cuts at: 142 2280 142
Size: 2138 3105
SanDI GG'GwC_CC
Cuts at: 326 326
Size: 5243
SapI GCTCTTCn'nnn_
Cuts at: 3266 3476 3266
Size: 210 5033
SbfI CC_TGCA'GG
Cuts at: 2290 2290
Size: 5243
ScaI AGT'ACT
Cuts at: 4760 4760
Size: 5243
SfcI C'TryA_G
Cuts at: 33 148 580 823 895 1582 1624 1812 2286
Size: 115 432 243 72 687 42 188 474
Cuts at: 2286 2971 3964 4155 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 687 685 474 432 243 191
188 115 72 42
SfoI GGC'GCC
Cuts at: 2675 2923 2675
Size: 248 4995
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5243
SmaI CCC'GGG
Cuts at: 352 363 2265 352
Size: 11 1902 3330
Fragments arranged by size:
3330 1902 11
SmlI C'TyrA_G
Cuts at: 133 2398 3313 3805 4067 133
Size: 2265 915 492 262 1309
Fragments arranged by size:
2265 1309 915 492 262
SnaBI TAC'GTA
Cuts at: 1058 1058
Size: 5243
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5243
SphI G_CATG'C
Cuts at: 2296 2796 2296
Size: 500 4743
StyI C'CwwG_G
Cuts at: 107 483 535 1332 2082 2377 2731 107
Size: 376 52 797 750 295 354 2619
Fragments arranged by size:
2619 797 750 376 354 295 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1082 1678 2826 2959 3418 3627 1082
Size: 596 1148 133 459 209 2698
Fragments arranged by size:
2698 1148 596 459 209 133
TatI w'GTAC_w
Cuts at: 4758 4758
Size: 5243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 867 899 1676 1708 1777 1809 4760 4792 867
Size: 32 777 32 69 32 2951 32 1318
Fragments arranged by size:
2951 1318 777 69 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 553 3040 553
Size: 2487 2756
XbaI T'CTAG_A
Cuts at: 2274 2274
Size: 5243
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1686 2007 2738 114
Size: 1572 321 731 2619
Fragments arranged by size:
2619 1572 731 321
XmaI C'CCGG_G
Cuts at: 350 361 2263 350
Size: 11 1902 3330
Fragments arranged by size:
3330 1902 11
XmnI GAAnn'nnTTC
Cuts at: 1741 1741
Size: 5243
ZraI GAC'GTC
Cuts at: 555 555
Size: 5243
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AloI AscI AvrII BcgI BfrBI
BglI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I CspCI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspXI PsrI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI