Restriction map for
pF1KE0370
(Circular) (Six-base) MAPSORT of: pF1KE0370.seq Check: 8742 from: 1 to: 4712
pF1KE0370 4712 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 649 1246 1303 649
Size: 597 57 4058
Fragments arranged by size:
4058 597 57
AatII G_ACGT'C
Cuts at: 290 851 290
Size: 561 4151
AccI GT'mk_AC
Cuts at: 1750 1750
Size: 4712
Acc65I G'GTAC_C
Cuts at: 1728 1728
Size: 4712
AclI AA'CG_TT
Cuts at: 4552 4552
Size: 4712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 2969 3716 3984 2537
Size: 432 747 268 3265
Fragments arranged by size:
3265 747 432 268
AflIII A'CryG_T
Cuts at: 2064 3168 4145 2064
Size: 1104 977 2631
Fragments arranged by size:
2631 1104 977
AgeI A'CCGG_T
Cuts at: 3100 3100
Size: 4712
AleI CACnn'nnGTG
Cuts at: 709 868 1174 709
Size: 159 306 4247
Fragments arranged by size:
4247 306 159
AlwNI CAG_nnn'CTG
Cuts at: 399 975 2017 3584 399
Size: 576 1042 1567 1527
Fragments arranged by size:
1567 1527 1042 576
ApaI G_GGCC'C
Cuts at: 411 1415 1546 411
Size: 1004 131 3577
Fragments arranged by size:
3577 1004 131
ApaLI G'TGCA_C
Cuts at: 3482 3482
Size: 4712
ApoI r'AATT_y
Cuts at: 1082 1716 4065 4321 1082
Size: 634 2349 256 1473
Fragments arranged by size:
2349 1473 634 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4712
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
AvaI C'yCGr_G
Cuts at: 1030 1732 1030
Size: 702 4010
BamHI G'GATC_C
Cuts at: 1247 1737 1247
Size: 490 4222
BanI G'GyrC_C
Cuts at: 369 1728 2142 2390 2425 369
Size: 1359 414 248 35 2656
Fragments arranged by size:
2656 1359 414 248 35
BanII G_rGCy'C
Cuts at: 254 411 1000 1211 1401 1415 1525 1546 1562
Size: 157 589 211 190 14 110 21 16
Cuts at: 1562 1726 2756 254
Size: 164 1030 2210
Fragments arranged by size:
2210 1030 589 211 190 164 157 110
21 16 14
BbeI G_GCGC'C
Cuts at: 2146 2394 2146
Size: 248 4464
BciVI GTATCCnnnnn_n'
Cuts at: 2610 3371 4712 2610
Size: 761 1341 2610
Fragments arranged by size:
2610 1341 761
BclI T'GATC_A
Cuts at: 2232 2232
Size: 4712
BglI GCCn_nnn'nGGC
Cuts at: 796 1198 796
Size: 402 4310
BglII A'GATC_T
Cuts at: 2227 4264 2227
Size: 2037 2675
BlpI GC'TnA_GC
Cuts at: 1390 1657 1824 1390
Size: 267 167 4278
Fragments arranged by size:
4278 267 167
Bme1580I G_kGCm'C
Cuts at: 372 411 1415 1546 2337 2430 3486 372
Size: 39 1004 131 791 93 1056 1598
Fragments arranged by size:
1598 1056 1004 791 131 93 39
BmgBI CAC'GTC
Cuts at: 1017 1227 1017
Size: 210 4502
BmrI ACTGGGnnnn_n'
Cuts at: 756 1924 2005 2340 4484 756
Size: 1168 81 335 2144 984
Fragments arranged by size:
2144 1168 984 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3079 3079
Size: 4712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 806 901 1297 1599 135
Size: 671 95 396 302 3248
Fragments arranged by size:
3248 671 396 302 95
Bpu10I CC'TnA_GC
Cuts at: 543 543
Size: 4712
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1384 1888 2767 3259 3557 1384
Size: 504 879 492 298 2539
Fragments arranged by size:
2539 879 504 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 346 4416 346
Size: 4070 642
BsaAI yAC'GTr
Cuts at: 582 2065 582
Size: 1483 3229
BsaBI GATnn'nnATC
Cuts at: 6 2226 4263 6
Size: 2220 2037 455
Fragments arranged by size:
2220 2037 455
BsaHI Gr'CG_yC
Cuts at: 287 848 2143 2391 3093 4589 287
Size: 561 1295 248 702 1496 410
Fragments arranged by size:
1496 1295 702 561 410 248
BsaWI w'CCGG_w
Cuts at: 1901 2422 3100 3374 3521 3932 1901
Size: 521 678 274 147 411 2681
Fragments arranged by size:
2681 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 779 809 1324 1354 1388 1418 1474 1504 779
Size: 30 515 30 34 30 56 30 3987
Fragments arranged by size:
3987 515 56 34 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 849 1552 849
Size: 703 4009
BseYI C'CCAG_C
Cuts at: 365 628 761 1555 2137 2895 3472 3968 365
Size: 263 133 794 582 758 577 496 1109
Fragments arranged by size:
1109 794 758 582 577 496 263 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 631 249
Size: 382 4330
BsiEI CG_ry'CG
Cuts at: 104 1055 2254 2300 3508 104
Size: 951 1199 46 1208 1308
Fragments arranged by size:
1308 1208 1199 951 46
BsiHKAI G_wGCw'C
Cuts at: 254 859 1211 1726 2504 2694 3486 254
Size: 605 352 515 778 190 792 1480
Fragments arranged by size:
1480 792 778 605 515 352 190
BsmI GAATG_Cn'
Cuts at: 128 128
Size: 4712
BsmBI CGTCTCn'nnnn_
Cuts at: 1443 1443
Size: 4712
Bsp1286I G_dGCh'C
Cuts at: 254 372 411 859 1000 1211 1401 1415 1525
Size: 118 39 448 141 211 190 14 110
Cuts at: 1525 1546 1562 1726 2337 2430 2504 2694 2756
Size: 21 16 164 611 93 74 190 62
Cuts at: 2756 3486 254
Size: 730 1480
Fragments arranged by size:
1480 730 611 448 211 190 190 164
141 118 110 93 74 62 39 21
16 14
BspEI T'CCGG_A
Cuts at: 3932 3932
Size: 4712
BspHI T'CATG_A
Cuts at: 3888 3888
Size: 4712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 649 1246 1303 1762 1976 2278 2659 649
Size: 597 57 459 214 302 381 2702
Fragments arranged by size:
2702 597 459 381 302 214 57
BsrBI CCG'CTC
Cuts at: 50 3004 3058 4542 4707 50
Size: 2954 54 1484 165 55
Fragments arranged by size:
2954 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1590 2624 1590
Size: 1034 3678
BsrFI r'CCGG_y
Cuts at: 619 2710 2891 3100 619
Size: 2091 181 209 2231
Fragments arranged by size:
2231 2091 209 181
BsrGI T'GTAC_A
Cuts at: 1163 1163
Size: 4712
BssSI C'ACGA_G
Cuts at: 2983 3341 2983
Size: 358 4354
BstAPI GCAn_nnn'nTGC
Cuts at: 975 2089 975
Size: 1114 3598
BstBI TT'CG_AA
Cuts at: 3073 3073
Size: 4712
BstYI r'GATC_y
Cuts at: 1 1247 1737 2204 2227 2562 2808 3820 3906
Size: 1246 490 467 23 335 246 1012 86
Cuts at: 3906 3918 4264 1
Size: 12 346 449
Fragments arranged by size:
1246 1012 490 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 440 710 803 107
Size: 333 270 93 4016
Fragments arranged by size:
4016 333 270 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1359 2662 2843 3006 1359
Size: 1303 181 163 3065
Fragments arranged by size:
3065 1303 181 163
BtsI GCAGTG_nn'
Cuts at: 266 976 1955 266
Size: 710 979 3023
Fragments arranged by size:
3023 979 710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1203 1238 1203
Size: 35 4677
DraI TTT'AAA
Cuts at: 1711 1711
Size: 4712
DrdI GACnn_nn'nnGTC
Cuts at: 2418 3276 2418
Size: 858 3854
EaeI y'GGCC_r
Cuts at: 1052 1502 2297 2471 2862 2889 4234 1052
Size: 450 795 174 391 27 1345 1530
Fragments arranged by size:
1530 1345 795 450 391 174 27
EagI C'GGCC_G
Cuts at: 1052 2297 1052
Size: 1245 3467
EarI CTCTTCn'nnn_
Cuts at: 829 928 1153 2735 2945 829
Size: 99 225 1582 210 2596
Fragments arranged by size:
2596 1582 225 210 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1247 1431 1627 2920 3230 3376 4522 1247
Size: 184 196 1293 310 146 1146 1437
Fragments arranged by size:
1437 1293 1146 310 196 184 146
EcoICRI GAG'CTC
Cuts at: 252 1209 1724 252
Size: 957 515 3240
Fragments arranged by size:
3240 957 515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 806 901 1297 1599 2537 2969 3716 3984
Size: 671 95 396 302 938 432 747 268
Cuts at: 3984 135
Size: 863
Fragments arranged by size:
938 863 747 671 432 396 302 268
95
EcoO109I rG'GnC_Cy
Cuts at: 407 408 765 1073 1286 1412 1543 1851 407
Size: 1 357 308 213 126 131 308 3268
Fragments arranged by size:
3268 357 308 308 213 131 126 1
EcoRI G'AATT_C
Cuts at: 1716 1716
Size: 4712
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 945 2731 945
Size: 1786 2926
FspI TGC'GCA
Cuts at: 2493 2493
Size: 4712
HaeII r_GCGC'y
Cuts at: 477 2146 2394 3416 4395 477
Size: 1669 248 1022 979 794
Fragments arranged by size:
1669 1022 979 794 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 899 931 1388 1420 2554 2584 2586 2616 899
Size: 32 457 32 1134 30 2 30 2995
Fragments arranged by size:
2995 1134 457 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1751 1751
Size: 4712
KasI G'GCGC_C
Cuts at: 2142 2390 2142
Size: 248 4464
KpnI G_GTAC'C
Cuts at: 1732 1732
Size: 4712
MluI A'CGCG_T
Cuts at: 4145 4145
Size: 4712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3383 3567 3383
Size: 184 4528
MscI TGG'CCA
Cuts at: 2473 2473
Size: 4712
MslI CAynn'nnrTG
Cuts at: 709 868 1174 1187 1378 1600 2701 2828 709
Size: 159 306 13 191 222 1101 127 2593
Fragments arranged by size:
2593 1101 306 222 191 159 127 13
MspA1I CmG'CkG
Cuts at: 124 325 399 518 1394 1823 2137 2497 3510
Size: 201 74 119 876 429 314 360 1013
Cuts at: 3510 3755 124
Size: 245 1081
Fragments arranged by size:
1081 1013 876 429 360 314 245 201
119 74
NaeI GCC'GGC
Cuts at: 2893 2893
Size: 4712
NarI GG'CG_CC
Cuts at: 2143 2391 2143
Size: 248 4464
NcoI C'CATG_G
Cuts at: 107 803 107
Size: 696 4016
NgoMIV G'CCGG_C
Cuts at: 2891 2891
Size: 4712
NspI r_CATG'y
Cuts at: 761 1599 1765 2265 3172 761
Size: 838 166 500 907 2301
Fragments arranged by size:
2301 907 838 500 166
PasI CC'CwG_GG
Cuts at: 1539 1666 1539
Size: 127 4585
PciI A'CATG_T
Cuts at: 3168 3168
Size: 4712
PflMI CCAn_nnn'nTGG
Cuts at: 623 761 623
Size: 138 4574
PmeI GTTT'AAAC
Cuts at: 1711 1711
Size: 4712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4680
PspOMI G'GGCC_C
Cuts at: 407 1411 1542 407
Size: 1004 131 3577
Fragments arranged by size:
3577 1004 131
PstI C_TGCA'G
Cuts at: 1759 2444 1759
Size: 685 4027
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4712
PvuII CAG'CTG
Cuts at: 399 2137 2497 399
Size: 1738 360 2614
Fragments arranged by size:
2614 1738 360
RsrII CG'GwC_CG
Cuts at: 2907 2907
Size: 4712
SacI G_AGCT'C
Cuts at: 254 1211 1726 254
Size: 957 515 3240
Fragments arranged by size:
3240 957 515
SalI G'TCGA_C
Cuts at: 1749 1749
Size: 4712
SapI GCTCTTCn'nnn_
Cuts at: 928 2735 2945 928
Size: 1807 210 2695
Fragments arranged by size:
2695 1807 210
SbfI CC_TGCA'GG
Cuts at: 1759 1759
Size: 4712
ScaI AGT'ACT
Cuts at: 262 4229 262
Size: 3967 745
SfcI C'TryA_G
Cuts at: 33 1526 1755 2440 3433 3624 33
Size: 1493 229 685 993 191 1121
Fragments arranged by size:
1493 1121 993 685 229 191
SfoI GGC'GCC
Cuts at: 2144 2392 2144
Size: 248 4464
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
SmaI CCC'GGG
Cuts at: 1032 1734 1032
Size: 702 4010
SmlI C'TyrA_G
Cuts at: 1363 1867 2782 3274 3536 1363
Size: 504 915 492 262 2539
Fragments arranged by size:
2539 915 504 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4712
SphI G_CATG'C
Cuts at: 1765 2265 1765
Size: 500 4212
StyI C'CwwG_G
Cuts at: 107 803 1846 2200 107
Size: 696 1043 354 2619
Fragments arranged by size:
2619 1043 696 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 370 738 1286 2295 2428 2887 3096 370
Size: 368 548 1009 133 459 209 1986
Fragments arranged by size:
1986 1009 548 459 368 209 133
TatI w'GTAC_w
Cuts at: 260 1163 4227 260
Size: 903 3064 745
Fragments arranged by size:
3064 903 745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 566 598 779 811 1474 1506 4229 4261 566
Size: 32 181 32 663 32 2723 32 1017
Fragments arranged by size:
2723 1017 663 181 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2509 2509
Size: 4712
XbaI T'CTAG_A
Cuts at: 1743 1743
Size: 4712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2207 2207
Size: 4712
XmaI C'CCGG_G
Cuts at: 1030 1732 1030
Size: 702 4010
ZraI GAC'GTC
Cuts at: 288 849 288
Size: 561 4151
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI BsiWI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI