Restriction map for
pF1KE0353
(Circular) (Six-base) MAPSORT of: pF1KE0353.seq Check: 1868 from: 1 to: 4742
pF1KE0353 4742 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1046 1780 1046
Size: 734 4008
Acc65I G'GTAC_C
Cuts at: 1758 1758
Size: 4742
AclI AA'CG_TT
Cuts at: 4582 4582
Size: 4742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1505 2567 2999 3746 4014 1505
Size: 1062 432 747 268 2233
Fragments arranged by size:
2233 1062 747 432 268
AflIII A'CryG_T
Cuts at: 2094 3198 4175 2094
Size: 1104 977 2661
Fragments arranged by size:
2661 1104 977
AgeI A'CCGG_T
Cuts at: 3130 3130
Size: 4742
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 282 776 282
Size: 494 4248
AlwNI CAG_nnn'CTG
Cuts at: 338 1152 1528 2047 3614 338
Size: 814 376 519 1567 1466
Fragments arranged by size:
1567 1466 814 519 376
ApaLI G'TGCA_C
Cuts at: 3512 3512
Size: 4742
ApoI r'AATT_y
Cuts at: 1726 1746 4095 4351 1726
Size: 20 2349 256 2117
Fragments arranged by size:
2349 2117 256 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 4742
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
AvaI C'yCGr_G
Cuts at: 1762 1762
Size: 4742
BamHI G'GATC_C
Cuts at: 536 1767 536
Size: 1231 3511
BanI G'GyrC_C
Cuts at: 1758 2172 2420 2455 1758
Size: 414 248 35 4045
Fragments arranged by size:
4045 414 248 35
BanII G_rGCy'C
Cuts at: 456 1756 2786 456
Size: 1300 1030 2412
Fragments arranged by size:
2412 1300 1030
BbeI G_GCGC'C
Cuts at: 2176 2424 2176
Size: 248 4494
BbsI GAAGACnn'nnnn_
Cuts at: 356 736 356
Size: 380 4362
BbvCI CC'TCA_GC
Cuts at: 230 230
Size: 4742
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 964 998 964
Size: 34 4708
BciVI GTATCCnnnnn_n'
Cuts at: 723 2640 3401 4742 723
Size: 1917 761 1341 723
Fragments arranged by size:
1917 1341 761 723
BclI T'GATC_A
Cuts at: 329 2262 329
Size: 1933 2809
BfrBI ATG'CAT
Cuts at: 313 313
Size: 4742
BglII A'GATC_T
Cuts at: 471 598 1308 1372 2257 4294 471
Size: 127 710 64 885 2037 919
Fragments arranged by size:
2037 919 885 710 127 64
BlpI GC'TnA_GC
Cuts at: 1854 1854
Size: 4742
Bme1580I G_kGCm'C
Cuts at: 1535 2367 2460 3516 1535
Size: 832 93 1056 2761
Fragments arranged by size:
2761 1056 832 93
BmrI ACTGGGnnnn_n'
Cuts at: 1503 1538 1954 2035 2370 4514 1503
Size: 35 416 81 335 2144 1731
Fragments arranged by size:
2144 1731 416 335 81 35
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3109 3109
Size: 4742
Bpu10I CC'TnA_GC
Cuts at: 230 406 230
Size: 176 4566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1918 2797 3289 3587 1918
Size: 879 492 298 3073
Fragments arranged by size:
3073 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4446 4446
Size: 4742
BsaAI yAC'GTr
Cuts at: 2095 2095
Size: 4742
BsaBI GATnn'nnATC
Cuts at: 6 2256 4293 6
Size: 2250 2037 455
Fragments arranged by size:
2250 2037 455
BsaHI Gr'CG_yC
Cuts at: 2173 2421 3123 4619 2173
Size: 248 702 1496 2296
Fragments arranged by size:
2296 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1931 2452 3130 3404 3551 3962 1931
Size: 521 678 274 147 411 2711
Fragments arranged by size:
2711 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 666 696 861 891 1212 1242 666
Size: 30 165 30 321 30 4166
Fragments arranged by size:
4166 321 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 499 528 538 499
Size: 29 10 4703
Fragments arranged by size:
4703 29 10
BseYI C'CCAG_C
Cuts at: 908 2167 2925 3502 3998 908
Size: 1259 758 577 496 1652
Fragments arranged by size:
1652 1259 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 1420
Size: 4742
BsiEI CG_ry'CG
Cuts at: 104 2284 2330 3538 104
Size: 2180 46 1208 1308
Fragments arranged by size:
2180 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1756 2534 2724 3516 1756
Size: 778 190 792 2982
Fragments arranged by size:
2982 792 778 190
BsmI GAATG_Cn'
Cuts at: 212 313 212
Size: 101 4641
Bsp1286I G_dGCh'C
Cuts at: 456 1535 1756 2367 2460 2534 2724 2786 3516
Size: 1079 221 611 93 74 190 62 730
Cuts at: 3516 456
Size: 1682
Fragments arranged by size:
1682 1079 730 611 221 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3962 3962
Size: 4742
BspHI T'CATG_A
Cuts at: 620 3918 620
Size: 3298 1444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1792 2006 2308 2689 1792
Size: 214 302 381 3845
Fragments arranged by size:
3845 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3034 3088 4572 4737 50
Size: 2984 54 1484 165 55
Fragments arranged by size:
2984 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 758 931 2654 758
Size: 173 1723 2846
Fragments arranged by size:
2846 1723 173
BsrFI r'CCGG_y
Cuts at: 2740 2921 3130 2740
Size: 181 209 4352
Fragments arranged by size:
4352 209 181
BssSI C'ACGA_G
Cuts at: 3013 3371 3013
Size: 358 4384
BstAPI GCAn_nnn'nTGC
Cuts at: 2119 2119
Size: 4742
BstBI TT'CG_AA
Cuts at: 3103 3103
Size: 4742
BstXI CCAn_nnnn'nTGG
Cuts at: 699 699
Size: 4742
BstYI r'GATC_y
Cuts at: 1 471 536 598 1308 1372 1767 2234 2257
Size: 470 65 62 710 64 395 467 23
Cuts at: 2257 2592 2838 3850 3936 3948 4294 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 710 470 467 449 395 346 335
246 86 65 64 62 23 12
Bsu36I CC'TnA_GG
Cuts at: 1290 1290
Size: 4742
BtgI C'CryG_G
Cuts at: 107 107
Size: 4742
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2692 2873 3036 2692
Size: 181 163 4398
Fragments arranged by size:
4398 181 163
BtsI GCAGTG_nn'
Cuts at: 855 919 1140 1320 1985 855
Size: 64 221 180 665 3612
Fragments arranged by size:
3612 665 221 180 64
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 149 184 149
Size: 35 4707
DraI TTT'AAA
Cuts at: 1741 1741
Size: 4742
DrdI GACnn_nn'nnGTC
Cuts at: 2448 3306 2448
Size: 858 3884
EaeI y'GGCC_r
Cuts at: 2327 2501 2892 2919 4264 2327
Size: 174 391 27 1345 2805
Fragments arranged by size:
2805 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2327 2327
Size: 4742
EarI CTCTTCn'nnn_
Cuts at: 199 526 933 2765 2975 199
Size: 327 407 1832 210 1966
Fragments arranged by size:
1966 1832 407 327 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2950 3260 3406 4552 2950
Size: 310 146 1146 3140
Fragments arranged by size:
3140 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1754 1754
Size: 4742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1505 2567 2999 3746 4014 1505
Size: 1062 432 747 268 2233
Fragments arranged by size:
2233 1062 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 554 554
Size: 4742
EcoO109I rG'GnC_Cy
Cuts at: 410 874 1278 1881 410
Size: 464 404 603 3271
Fragments arranged by size:
3271 603 464 404
EcoRI G'AATT_C
Cuts at: 1746 1746
Size: 4742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1416 2761 1416
Size: 1345 3397
FspI TGC'GCA
Cuts at: 2523 2523
Size: 4742
HaeII r_GCGC'y
Cuts at: 2176 2424 3446 4425 2176
Size: 248 1022 979 2493
Fragments arranged by size:
2493 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 450 482 1159 1191 2584 2614 2616
Size: 32 180 32 677 32 1393 30 2
Cuts at: 2616 2646 238
Size: 30 2334
Fragments arranged by size:
2334 1393 677 180 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1656 1781 1656
Size: 125 4617
KasI G'GCGC_C
Cuts at: 2172 2420 2172
Size: 248 4494
KpnI G_GTAC'C
Cuts at: 1762 1762
Size: 4742
MluI A'CGCG_T
Cuts at: 4175 4175
Size: 4742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3413 3597 3413
Size: 184 4558
MscI TGG'CCA
Cuts at: 2503 2503
Size: 4742
MslI CAynn'nnrTG
Cuts at: 358 2731 2858 358
Size: 2373 127 2242
Fragments arranged by size:
2373 2242 127
MspA1I CmG'CkG
Cuts at: 1853 2167 2527 3540 3785 1853
Size: 314 360 1013 245 2810
Fragments arranged by size:
2810 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2923 2923
Size: 4742
NarI GG'CG_CC
Cuts at: 2173 2421 2173
Size: 248 4494
NcoI C'CATG_G
Cuts at: 107 107
Size: 4742
NgoMIV G'CCGG_C
Cuts at: 2921 2921
Size: 4742
NsiI A_TGCA'T
Cuts at: 315 315
Size: 4742
NspI r_CATG'y
Cuts at: 1795 2295 3202 1795
Size: 500 907 3335
Fragments arranged by size:
3335 907 500
PasI CC'CwG_GG
Cuts at: 1124 1124
Size: 4742
PciI A'CATG_T
Cuts at: 3198 3198
Size: 4742
PmeI GTTT'AAAC
Cuts at: 1741 1741
Size: 4742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 4710
PpuMI rG'GwC_Cy
Cuts at: 1278 1278
Size: 4742
PsiI TTA'TAA
Cuts at: 836 836
Size: 4742
PstI C_TGCA'G
Cuts at: 1190 1327 1789 2474 1190
Size: 137 462 685 3458
Fragments arranged by size:
3458 685 462 137
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4742
PvuII CAG'CTG
Cuts at: 2167 2527 2167
Size: 360 4382
RsrII CG'GwC_CG
Cuts at: 2937 2937
Size: 4742
SacI G_AGCT'C
Cuts at: 1756 1756
Size: 4742
SalI G'TCGA_C
Cuts at: 1779 1779
Size: 4742
SapI GCTCTTCn'nnn_
Cuts at: 933 2765 2975 933
Size: 1832 210 2700
Fragments arranged by size:
2700 1832 210
SbfI CC_TGCA'GG
Cuts at: 1789 1789
Size: 4742
ScaI AGT'ACT
Cuts at: 4259 4259
Size: 4742
SfcI C'TryA_G
Cuts at: 33 1186 1323 1785 2470 3463 3654 33
Size: 1153 137 462 685 993 191 1121
Fragments arranged by size:
1153 1121 993 685 462 191 137
SfoI GGC'GCC
Cuts at: 2174 2422 2174
Size: 248 4494
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
SmaI CCC'GGG
Cuts at: 1764 1764
Size: 4742
SmlI C'TyrA_G
Cuts at: 1897 2812 3304 3566 1897
Size: 915 492 262 3073
Fragments arranged by size:
3073 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4742
SphI G_CATG'C
Cuts at: 1795 2295 1795
Size: 500 4242
SspI AAT'ATT
Cuts at: 797 898 797
Size: 101 4641
StyI C'CwwG_G
Cuts at: 107 1030 1876 2230 107
Size: 923 846 354 2619
Fragments arranged by size:
2619 923 846 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 118 2325 2458 2917 3126 118
Size: 2207 133 459 209 1734
Fragments arranged by size:
2207 1734 459 209 133
TatI w'GTAC_w
Cuts at: 4257 4257
Size: 4742
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 666 698 4259 4291 666
Size: 32 3561 32 1117
Fragments arranged by size:
3561 1117 32 32
Tth111I GACn'n_nGTC
Cuts at: 2539 2539
Size: 4742
XbaI T'CTAG_A
Cuts at: 1773 1773
Size: 4742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 603 1177 2237 603
Size: 574 1060 3108
Fragments arranged by size:
3108 1060 574
XmaI C'CCGG_G
Cuts at: 1762 1762
Size: 4742
XmnI GAAnn'nnTTC
Cuts at: 797 797
Size: 4742
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BglI BmgBI BmtI BpmI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PflMI PfoI PmlI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI