Restriction map for
pF1KE0345
(Circular) (Six-base) MAPSORT of: pF1KE0345.seq Check: 704 from: 1 to: 4820
pF1KE0345 4820 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1431 1431
Size: 4820
AccI GT'mk_AC
Cuts at: 1858 1858
Size: 4820
Acc65I G'GTAC_C
Cuts at: 1410 1836 1410
Size: 426 4394
AclI AA'CG_TT
Cuts at: 4660 4660
Size: 4820
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1362 2645 3077 3824 4092 1362
Size: 1283 432 747 268 2090
Fragments arranged by size:
2090 1283 747 432 268
AflIII A'CryG_T
Cuts at: 2172 3276 4253 2172
Size: 1104 977 2739
Fragments arranged by size:
2739 1104 977
AgeI A'CCGG_T
Cuts at: 3208 3208
Size: 4820
AleI CACnn'nnGTG
Cuts at: 1648 1648
Size: 4820
AlwNI CAG_nnn'CTG
Cuts at: 1632 1678 2125 3692 1632
Size: 46 447 1567 2760
Fragments arranged by size:
2760 1567 447 46
ApaI G_GGCC'C
Cuts at: 959 1176 959
Size: 217 4603
ApaLI G'TGCA_C
Cuts at: 3590 3590
Size: 4820
ApoI r'AATT_y
Cuts at: 1824 4173 4429 1824
Size: 2349 256 2215
Fragments arranged by size:
2349 2215 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4820
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4820
AvaI C'yCGr_G
Cuts at: 1004 1024 1219 1840 1004
Size: 20 195 621 3984
Fragments arranged by size:
3984 621 195 20
BamHI G'GATC_C
Cuts at: 1349 1845 1349
Size: 496 4324
BanI G'GyrC_C
Cuts at: 507 778 1410 1697 1836 2250 2498 2533 507
Size: 271 632 287 139 414 248 35 2794
Fragments arranged by size:
2794 632 414 287 271 248 139 35
BanII G_rGCy'C
Cuts at: 338 570 959 1024 1176 1613 1834 2864 338
Size: 232 389 65 152 437 221 1030 2294
Fragments arranged by size:
2294 1030 437 389 232 221 152 65
BbeI G_GCGC'C
Cuts at: 2254 2502 2254
Size: 248 4572
BbvCI CC'TCA_GC
Cuts at: 189 410 857 189
Size: 221 447 4152
Fragments arranged by size:
4152 447 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 342 376 1760 1794 342
Size: 34 1384 34 3368
Fragments arranged by size:
3368 1384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2718 3479 4820 2718
Size: 761 1341 2718
Fragments arranged by size:
2718 1341 761
BclI T'GATC_A
Cuts at: 2340 2340
Size: 4820
BglI GCCn_nnn'nGGC
Cuts at: 1493 1493
Size: 4820
BglII A'GATC_T
Cuts at: 1178 2335 4372 1178
Size: 1157 2037 1626
Fragments arranged by size:
2037 1626 1157
BlpI GC'TnA_GC
Cuts at: 881 949 1932 881
Size: 68 983 3769
Fragments arranged by size:
3769 983 68
Bme1580I G_kGCm'C
Cuts at: 783 959 1176 1249 1471 1531 2445 2538 3594
Size: 176 217 73 222 60 914 93 1056
Cuts at: 3594 783
Size: 2009
Fragments arranged by size:
2009 1056 914 222 217 176 93 73
60
BmgBI CAC'GTC
Cuts at: 921 1590 921
Size: 669 4151
BmrI ACTGGGnnnn_n'
Cuts at: 932 2032 2113 2448 4592 932
Size: 1100 81 335 2144 1160
Fragments arranged by size:
2144 1160 1100 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3187 3187
Size: 4820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1708 1770 1708
Size: 62 4758
Bpu10I CC'TnA_GC
Cuts at: 189 410 857 189
Size: 221 447 4152
Fragments arranged by size:
4152 447 221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1996 2875 3367 3665 1996
Size: 879 492 298 3151
Fragments arranged by size:
3151 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1684 4524 1684
Size: 2840 1980
BsaAI yAC'GTr
Cuts at: 2173 2173
Size: 4820
BsaBI GATnn'nnATC
Cuts at: 6 2334 4371 6
Size: 2328 2037 455
Fragments arranged by size:
2328 2037 455
BsaHI Gr'CG_yC
Cuts at: 2251 2499 3201 4697 2251
Size: 248 702 1496 2374
Fragments arranged by size:
2374 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1067 1502 2009 2530 3208 3482 3629 4040 1067
Size: 435 507 521 678 274 147 411 1847
Fragments arranged by size:
1847 678 521 507 435 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 908 938 985 1015 1129 1159 1189 1219 1306
Size: 30 47 30 114 30 30 30 87
Cuts at: 1306 1336 908
Size: 30 4392
Fragments arranged by size:
4392 114 87 47 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1621 1621
Size: 4820
BseYI C'CCAG_C
Cuts at: 129 583 1163 1387 1522 1766 2245 3003 3580
Size: 454 580 224 135 244 479 758 577
Cuts at: 3580 4076 129
Size: 496 873
Fragments arranged by size:
873 758 580 577 496 479 454 244
224 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1535 1603 1535
Size: 68 4752
BsiEI CG_ry'CG
Cuts at: 104 839 2362 2408 3616 104
Size: 735 1523 46 1208 1308
Fragments arranged by size:
1523 1308 1208 735 46
BsiHKAI G_wGCw'C
Cuts at: 259 896 1834 2612 2802 3594 259
Size: 637 938 778 190 792 1485
Fragments arranged by size:
1485 938 792 778 637 190
BsmI GAATG_Cn'
Cuts at: 348 348
Size: 4820
BsmBI CGTCTCn'nnnn_
Cuts at: 754 911 754
Size: 157 4663
Bsp1286I G_dGCh'C
Cuts at: 259 338 570 783 896 959 1024 1176 1249
Size: 79 232 213 113 63 65 152 73
Cuts at: 1249 1471 1531 1613 1834 2445 2538 2612 2802
Size: 222 60 82 221 611 93 74 190
Cuts at: 2802 2864 3594 259
Size: 62 730 1485
Fragments arranged by size:
1485 730 611 232 222 221 213 190
152 113 93 82 79 74 73 65
63 62 60
BspEI T'CCGG_A
Cuts at: 1502 4040 1502
Size: 2538 2282
BspHI T'CATG_A
Cuts at: 338 3996 338
Size: 3658 1162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1431 1870 2084 2386 2767 1431
Size: 439 214 302 381 3484
Fragments arranged by size:
3484 439 381 302 214
BsrBI CCG'CTC
Cuts at: 50 948 1334 1455 3112 3166 4650 4815 50
Size: 898 386 121 1657 54 1484 165 55
Fragments arranged by size:
1657 1484 898 386 165 121 55 54
BsrDI GCAATG_nn'
Cuts at: 311 2732 311
Size: 2421 2399
BsrFI r'CCGG_y
Cuts at: 1807 2818 2999 3208 1807
Size: 1011 181 209 3419
Fragments arranged by size:
3419 1011 209 181
BsrGI T'GTAC_A
Cuts at: 218 929 218
Size: 711 4109
BssSI C'ACGA_G
Cuts at: 3091 3449 3091
Size: 358 4462
BstAPI GCAn_nnn'nTGC
Cuts at: 313 1678 2197 313
Size: 1365 519 2936
Fragments arranged by size:
2936 1365 519
BstBI TT'CG_AA
Cuts at: 3181 3181
Size: 4820
BstXI CCAn_nnnn'nTGG
Cuts at: 550 1113 1554 550
Size: 563 441 3816
Fragments arranged by size:
3816 563 441
BstYI r'GATC_y
Cuts at: 1 518 1178 1349 1845 2312 2335 2670 2916
Size: 517 660 171 496 467 23 335 246
Cuts at: 2916 3928 4014 4026 4372 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 660 517 496 467 449 346 335
246 171 86 23 12
BtgI C'CryG_G
Cuts at: 143 233 239 614 1541 1601 143
Size: 90 6 375 927 60 3362
Fragments arranged by size:
3362 927 375 90 60 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1713 2770 2951 3114 1713
Size: 1057 181 163 3419
Fragments arranged by size:
3419 1057 181 163
BtsI GCAGTG_nn'
Cuts at: 259 277 1247 1287 1547 1679 2063 259
Size: 18 970 40 260 132 384 3016
Fragments arranged by size:
3016 970 384 260 132 40 18
DraI TTT'AAA
Cuts at: 1819 1819
Size: 4820
DrdI GACnn_nn'nnGTC
Cuts at: 1498 2526 3384 1498
Size: 1028 858 2934
Fragments arranged by size:
2934 1028 858
EaeI y'GGCC_r
Cuts at: 140 236 767 836 1494 1544 1556 2405 2579
Size: 96 531 69 658 50 12 849 174
Cuts at: 2579 2970 2997 4342 140
Size: 391 27 1345 618
Fragments arranged by size:
1345 849 658 618 531 391 174 96
69 50 27 12
EagI C'GGCC_G
Cuts at: 836 2405 836
Size: 1569 3251
EarI CTCTTCn'nnn_
Cuts at: 379 580 2843 3053 379
Size: 201 2263 210 2146
Fragments arranged by size:
2263 2146 210 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1281 3028 3338 3484 4630 1281
Size: 1747 310 146 1146 1471
Fragments arranged by size:
1747 1471 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1832 1832
Size: 4820
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1362 1708 1770 2645 3077 3824 4092 1362
Size: 346 62 875 432 747 268 2090
Fragments arranged by size:
2090 875 747 432 346 268 62
EcoO109I rG'GnC_Cy
Cuts at: 861 1035 1088 1101 1959 861
Size: 174 53 13 858 3722
Fragments arranged by size:
3722 858 174 53 13
EcoRI G'AATT_C
Cuts at: 1824 1824
Size: 4820
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 4820
FspI TGC'GCA
Cuts at: 2601 2601
Size: 4820
HaeII r_GCGC'y
Cuts at: 2254 2502 3524 4503 2254
Size: 248 1022 979 2571
Fragments arranged by size:
2571 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 277 309 1366 1398 2662 2692 2694 2724 277
Size: 32 1057 32 1264 30 2 30 2373
Fragments arranged by size:
2373 1264 1057 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1859 1859
Size: 4820
KasI G'GCGC_C
Cuts at: 2250 2498 2250
Size: 248 4572
KpnI G_GTAC'C
Cuts at: 1414 1840 1414
Size: 426 4394
MluI A'CGCG_T
Cuts at: 4253 4253
Size: 4820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3491 3675 3491
Size: 184 4636
MscI TGG'CCA
Cuts at: 769 1558 2581 769
Size: 789 1023 3008
Fragments arranged by size:
3008 1023 789
MslI CAynn'nnrTG
Cuts at: 1648 1732 2809 2936 1648
Size: 84 1077 127 3532
Fragments arranged by size:
3532 1077 127 84
MspA1I CmG'CkG
Cuts at: 241 546 594 744 856 900 953 1346 1774
Size: 305 48 150 112 44 53 393 428
Cuts at: 1774 1931 2245 2605 3618 3863 241
Size: 157 314 360 1013 245 1198
Fragments arranged by size:
1198 1013 428 393 360 314 305 245
157 150 112 53 48 44
NaeI GCC'GGC
Cuts at: 1809 3001 1809
Size: 1192 3628
NarI GG'CG_CC
Cuts at: 2251 2499 2251
Size: 248 4572
NgoMIV G'CCGG_C
Cuts at: 1807 2999 1807
Size: 1192 3628
NspI r_CATG'y
Cuts at: 936 1873 2373 3280 936
Size: 937 500 907 2476
Fragments arranged by size:
2476 937 907 500
PasI CC'CwG_GG
Cuts at: 563 563
Size: 4820
PciI A'CATG_T
Cuts at: 3276 3276
Size: 4820
PflMI CCAn_nnn'nTGG
Cuts at: 354 354
Size: 4820
PfoI T'CCnGG_A
Cuts at: 1786 1786
Size: 4820
PmeI GTTT'AAAC
Cuts at: 1819 1819
Size: 4820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 3959
Size: 32 4788
PpuMI rG'GwC_Cy
Cuts at: 861 1088 1101 861
Size: 227 13 4580
Fragments arranged by size:
4580 227 13
PspOMI G'GGCC_C
Cuts at: 955 1172 955
Size: 217 4603
PstI C_TGCA'G
Cuts at: 1683 1867 2552 1683
Size: 184 685 3951
Fragments arranged by size:
3951 685 184
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4820
PvuII CAG'CTG
Cuts at: 594 744 856 1774 2245 2605 594
Size: 150 112 918 471 360 2809
Fragments arranged by size:
2809 918 471 360 150 112
RsrII CG'GwC_CG
Cuts at: 653 3015 653
Size: 2362 2458
SacI G_AGCT'C
Cuts at: 1834 1834
Size: 4820
SacII CC_GC'GG
Cuts at: 242 242
Size: 4820
SalI G'TCGA_C
Cuts at: 1857 1857
Size: 4820
SanDI GG'GwC_CC
Cuts at: 1101 1101
Size: 4820
SapI GCTCTTCn'nnn_
Cuts at: 2843 3053 2843
Size: 210 4610
SbfI CC_TGCA'GG
Cuts at: 1867 1867
Size: 4820
ScaI AGT'ACT
Cuts at: 4337 4337
Size: 4820
SfcI C'TryA_G
Cuts at: 33 1679 1863 2548 3541 3732 33
Size: 1646 184 685 993 191 1121
Fragments arranged by size:
1646 1121 993 685 191 184
SfiI GGCCn_nnn'nGGCC
Cuts at: 1493 1493
Size: 4820
SfoI GGC'GCC
Cuts at: 2252 2500 2252
Size: 248 4572
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4820
SmaI CCC'GGG
Cuts at: 1006 1842 1006
Size: 836 3984
SmlI C'TyrA_G
Cuts at: 1975 2890 3382 3644 1975
Size: 915 492 262 3151
Fragments arranged by size:
3151 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4820
SphI G_CATG'C
Cuts at: 1873 2373 1873
Size: 500 4320
StuI AGG'CCT
Cuts at: 787 787
Size: 4820
StyI C'CwwG_G
Cuts at: 197 1325 1954 2308 197
Size: 1128 629 354 2709
Fragments arranged by size:
2709 1128 629 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1723 2403 2536 2995 3204 1723
Size: 680 133 459 209 3339
Fragments arranged by size:
3339 680 459 209 133
TatI w'GTAC_w
Cuts at: 218 929 4335 218
Size: 711 3406 703
Fragments arranged by size:
3406 711 703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 908 940 1189 1221 4337 4369 908
Size: 32 249 32 3116 32 1359
Fragments arranged by size:
3116 1359 249 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2617 2617
Size: 4820
XbaI T'CTAG_A
Cuts at: 1851 1851
Size: 4820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 231 591 2315 231
Size: 360 1724 2736
Fragments arranged by size:
2736 1724 360
XmaI C'CCGG_G
Cuts at: 1004 1840 1004
Size: 836 3984
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BfrBI BmtI BsiWI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI