Restriction map for
pF1KE0338
(Circular) (Six-base) MAPSORT of: pF1KE0338.seq Check: 4234 from: 1 to: 4628
pF1KE0338 4628 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1666 1666
Size: 4628
Acc65I G'GTAC_C
Cuts at: 1644 1644
Size: 4628
AclI AA'CG_TT
Cuts at: 4468 4468
Size: 4628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 689 1048 2453 2885 3632 3900 449
Size: 240 359 1405 432 747 268 1177
Fragments arranged by size:
1405 1177 747 432 359 268 240
AflII C'TTAA_G
Cuts at: 645 645
Size: 4628
AflIII A'CryG_T
Cuts at: 1143 1980 3084 4061 1143
Size: 837 1104 977 1710
Fragments arranged by size:
1710 1104 977 837
AgeI A'CCGG_T
Cuts at: 3016 3016
Size: 4628
AlwNI CAG_nnn'CTG
Cuts at: 1045 1933 3500 1045
Size: 888 1567 2173
Fragments arranged by size:
2173 1567 888
ApaLI G'TGCA_C
Cuts at: 3398 3398
Size: 4628
ApoI r'AATT_y
Cuts at: 1632 3981 4237 1632
Size: 2349 256 2023
Fragments arranged by size:
2349 2023 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4628
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 1412 1648 1412
Size: 236 4392
BamHI G'GATC_C
Cuts at: 1234 1653 1234
Size: 419 4209
BanI G'GyrC_C
Cuts at: 1315 1644 2058 2306 2341 1315
Size: 329 414 248 35 3602
Fragments arranged by size:
3602 414 329 248 35
BanII G_rGCy'C
Cuts at: 1642 2672 1642
Size: 1030 3598
BbeI G_GCGC'C
Cuts at: 2062 2310 2062
Size: 248 4380
BbsI GAAGACnn'nnnn_
Cuts at: 677 1104 677
Size: 427 4201
BciVI GTATCCnnnnn_n'
Cuts at: 450 2526 3287 4628 450
Size: 2076 761 1341 450
Fragments arranged by size:
2076 1341 761 450
BclI T'GATC_A
Cuts at: 1613 2148 1613
Size: 535 4093
BglII A'GATC_T
Cuts at: 2143 4180 2143
Size: 2037 2591
BlpI GC'TnA_GC
Cuts at: 1740 1740
Size: 4628
Bme1580I G_kGCm'C
Cuts at: 191 2253 2346 3402 191
Size: 2062 93 1056 1417
Fragments arranged by size:
2062 1417 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1840 1921 2256 4400 1840
Size: 81 335 2144 2068
Fragments arranged by size:
2144 2068 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 4628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 253 186
Size: 67 4561
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1804 2683 3175 3473 1804
Size: 879 492 298 2959
Fragments arranged by size:
2959 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1363 1593 4332 1363
Size: 230 2739 1659
Fragments arranged by size:
2739 1659 230
BsaAI yAC'GTr
Cuts at: 1981 1981
Size: 4628
BsaBI GATnn'nnATC
Cuts at: 6 2142 4179 6
Size: 2136 2037 455
Fragments arranged by size:
2136 2037 455
BsaHI Gr'CG_yC
Cuts at: 2059 2307 3009 4505 2059
Size: 248 702 1496 2182
Fragments arranged by size:
2182 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1817 2338 3016 3290 3437 3848 1817
Size: 521 678 274 147 411 2597
Fragments arranged by size:
2597 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 628 703 1244 628
Size: 75 541 4012
Fragments arranged by size:
4012 541 75
BseYI C'CCAG_C
Cuts at: 1045 2053 2811 3388 3884 1045
Size: 1008 758 577 496 1789
Fragments arranged by size:
1789 1008 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2170 2216 3424 104
Size: 2066 46 1208 1308
Fragments arranged by size:
2066 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 683 996 1307 1642 2420 2610 3402 683
Size: 313 311 335 778 190 792 1909
Fragments arranged by size:
1909 792 778 335 313 311 190
BsmI GAATG_Cn'
Cuts at: 267 603 267
Size: 336 4292
Bsp1286I G_dGCh'C
Cuts at: 191 683 996 1307 1642 2253 2346 2420 2610
Size: 492 313 311 335 611 93 74 190
Cuts at: 2610 2672 3402 191
Size: 62 730 1417
Fragments arranged by size:
1417 730 611 492 335 313 311 190
93 74 62
BspEI T'CCGG_A
Cuts at: 3848 3848
Size: 4628
BspHI T'CATG_A
Cuts at: 3804 3804
Size: 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 189 1567 1678 1892 2194 2575 189
Size: 1378 111 214 302 381 2242
Fragments arranged by size:
2242 1378 381 302 214 111
BsrBI CCG'CTC
Cuts at: 50 1070 1396 2920 2974 4458 4623 50
Size: 1020 326 1524 54 1484 165 55
Fragments arranged by size:
1524 1484 1020 326 165 55 54
BsrDI GCAATG_nn'
Cuts at: 324 1169 1268 2540 324
Size: 845 99 1272 2412
Fragments arranged by size:
2412 1272 845 99
BsrFI r'CCGG_y
Cuts at: 2626 2807 3016 2626
Size: 181 209 4238
Fragments arranged by size:
4238 209 181
BssSI C'ACGA_G
Cuts at: 2899 3257 2899
Size: 358 4270
BstAPI GCAn_nnn'nTGC
Cuts at: 2005 2005
Size: 4628
BstBI TT'CG_AA
Cuts at: 2989 2989
Size: 4628
BstXI CCAn_nnnn'nTGG
Cuts at: 609 1291 1570 609
Size: 682 279 3667
Fragments arranged by size:
3667 682 279
BstYI r'GATC_y
Cuts at: 1 1234 1653 2120 2143 2478 2724 3736 3822
Size: 1233 419 467 23 335 246 1012 86
Cuts at: 3822 3834 4180 1
Size: 12 346 449
Fragments arranged by size:
1233 1012 467 449 419 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1319 1319
Size: 4628
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2578 2759 2922 2578
Size: 181 163 4284
Fragments arranged by size:
4284 181 163
BtsI GCAGTG_nn'
Cuts at: 681 1871 681
Size: 1190 3438
ClaI AT'CG_AT
Cuts at: 348 348
Size: 4628
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DrdI GACnn_nn'nnGTC
Cuts at: 2334 3192 2334
Size: 858 3770
EaeI y'GGCC_r
Cuts at: 2213 2387 2778 2805 4150 2213
Size: 174 391 27 1345 2691
Fragments arranged by size:
2691 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2213 2213
Size: 4628
EarI CTCTTCn'nnn_
Cuts at: 946 1077 1508 2651 2861 946
Size: 131 431 1143 210 2713
Fragments arranged by size:
2713 1143 431 210 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2836 3146 3292 4438 2836
Size: 310 146 1146 3026
Fragments arranged by size:
3026 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1640 1640
Size: 4628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 253 449 689 1048 2453 2885 3632 3900
Size: 67 196 240 359 1405 432 747 268
Cuts at: 3900 186
Size: 914
Fragments arranged by size:
1405 914 747 432 359 268 240 196
67
EcoO109I rG'GnC_Cy
Cuts at: 767 1767 767
Size: 1000 3628
EcoRI G'AATT_C
Cuts at: 1632 1632
Size: 4628
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 198 2647 198
Size: 2449 2179
FspI TGC'GCA
Cuts at: 2409 2409
Size: 4628
HaeII r_GCGC'y
Cuts at: 2062 2310 3332 4311 2062
Size: 248 1022 979 2379
Fragments arranged by size:
2379 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2470 2500 2502 2532 2470
Size: 30 2 30 4566
Fragments arranged by size:
4566 30 30 2
HincII GTy'rAC
Cuts at: 1065 1667 1065
Size: 602 4026
KasI G'GCGC_C
Cuts at: 2058 2306 2058
Size: 248 4380
KpnI G_GTAC'C
Cuts at: 1648 1648
Size: 4628
MfeI C'AATT_G
Cuts at: 1604 1604
Size: 4628
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3299 3483 3299
Size: 184 4444
MscI TGG'CCA
Cuts at: 2389 2389
Size: 4628
MslI CAynn'nnrTG
Cuts at: 592 2617 2744 592
Size: 2025 127 2476
Fragments arranged by size:
2476 2025 127
MspA1I CmG'CkG
Cuts at: 1042 1739 2053 2413 3426 3671 1042
Size: 697 314 360 1013 245 1999
Fragments arranged by size:
1999 1013 697 360 314 245
NaeI GCC'GGC
Cuts at: 2809 2809
Size: 4628
NarI GG'CG_CC
Cuts at: 2059 2307 2059
Size: 248 4380
NcoI C'CATG_G
Cuts at: 1319 1319
Size: 4628
NgoMIV G'CCGG_C
Cuts at: 2807 2807
Size: 4628
NspI r_CATG'y
Cuts at: 198 1147 1192 1681 2181 3088 198
Size: 949 45 489 500 907 1738
Fragments arranged by size:
1738 949 907 500 489 45
PasI CC'CwG_GG
Cuts at: 771 771
Size: 4628
PciI A'CATG_T
Cuts at: 1143 3084 1143
Size: 1941 2687
PflMI CCAn_nnn'nTGG
Cuts at: 807 807
Size: 4628
PfoI T'CCnGG_A
Cuts at: 1442 1442
Size: 4628
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 121 153 3767 3799 121
Size: 32 3614 32 950
Fragments arranged by size:
3614 950 32 32
PpuMI rG'GwC_Cy
Cuts at: 767 767
Size: 4628
PstI C_TGCA'G
Cuts at: 137 710 1675 2360 137
Size: 573 965 685 2405
Fragments arranged by size:
2405 965 685 573
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 1042 2053 2413 1042
Size: 1011 360 3257
Fragments arranged by size:
3257 1011 360
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 1642 1642
Size: 4628
SalI G'TCGA_C
Cuts at: 1665 1665
Size: 4628
SanDI GG'GwC_CC
Cuts at: 767 767
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 1077 2651 2861 1077
Size: 1574 210 2844
Fragments arranged by size:
2844 1574 210
SbfI CC_TGCA'GG
Cuts at: 1675 1675
Size: 4628
ScaI AGT'ACT
Cuts at: 4145 4145
Size: 4628
SfcI C'TryA_G
Cuts at: 33 133 706 1329 1671 2356 3349 3540 33
Size: 100 573 623 342 685 993 191 1121
Fragments arranged by size:
1121 993 685 623 573 342 191 100
SfoI GGC'GCC
Cuts at: 2060 2308 2060
Size: 248 4380
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 1650 1650
Size: 4628
SmlI C'TyrA_G
Cuts at: 645 1783 2698 3190 3452 645
Size: 1138 915 492 262 1821
Fragments arranged by size:
1821 1138 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 1681 2181 1681
Size: 500 4128
StuI AGG'CCT
Cuts at: 619 752 619
Size: 133 4495
StyI C'CwwG_G
Cuts at: 620 1106 1319 1762 2116 620
Size: 486 213 443 354 3132
Fragments arranged by size:
3132 486 443 354 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1512 2211 2344 2803 3012 1512
Size: 699 133 459 209 3128
Fragments arranged by size:
3128 699 459 209 133
TatI w'GTAC_w
Cuts at: 1221 4143 1221
Size: 2922 1706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1286 1318 4145 4177 1286
Size: 32 2827 32 1737
Fragments arranged by size:
2827 1737 32 32
Tth111I GACn'n_nGTC
Cuts at: 2425 2425
Size: 4628
XbaI T'CTAG_A
Cuts at: 1659 1659
Size: 4628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 379 562 924 1506 2123 379
Size: 183 362 582 617 2884
Fragments arranged by size:
2884 617 582 362 183
XmaI C'CCGG_G
Cuts at: 1648 1648
Size: 4628
XmnI GAAnn'nnTTC
Cuts at: 181 1002 181
Size: 821 3807
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI