Restriction map for
pF1KE0330
(Circular) (Six-base) MAPSORT of: pF1KE0330.seq Check: 9887 from: 1 to: 4937
pF1KE0330 4937 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1975 1975
Size: 4937
Acc65I G'GTAC_C
Cuts at: 1953 1953
Size: 4937
AclI AA'CG_TT
Cuts at: 4777 4777
Size: 4937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 917 1201 1363 1443 2762 3194 3941 4209 917
Size: 284 162 80 1319 432 747 268 1645
Fragments arranged by size:
1645 1319 747 432 284 268 162 80
AflIII A'CryG_T
Cuts at: 2289 3393 4370 2289
Size: 1104 977 2856
Fragments arranged by size:
2856 1104 977
AgeI A'CCGG_T
Cuts at: 3325 3325
Size: 4937
AhdI GACnn_n'nnGTC
Cuts at: 1400 1400
Size: 4937
AlwNI CAG_nnn'CTG
Cuts at: 366 398 2242 3809 366
Size: 32 1844 1567 1494
Fragments arranged by size:
1844 1567 1494 32
ApaI G_GGCC'C
Cuts at: 219 219
Size: 4937
ApaLI G'TGCA_C
Cuts at: 3707 3707
Size: 4937
ApoI r'AATT_y
Cuts at: 682 1408 1941 4290 4546 682
Size: 726 533 2349 256 1073
Fragments arranged by size:
2349 1073 726 533 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4937
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4937
AvaI C'yCGr_G
Cuts at: 152 309 1957 152
Size: 157 1648 3132
Fragments arranged by size:
3132 1648 157
AvrII C'CTAG_G
Cuts at: 687 687
Size: 4937
BamHI G'GATC_C
Cuts at: 133 1962 133
Size: 1829 3108
BanI G'GyrC_C
Cuts at: 254 1626 1787 1953 2367 2615 2650 254
Size: 1372 161 166 414 248 35 2541
Fragments arranged by size:
2541 1372 414 248 166 161 35
BanII G_rGCy'C
Cuts at: 219 332 557 895 1421 1505 1951 2981 219
Size: 113 225 338 526 84 446 1030 2175
Fragments arranged by size:
2175 1030 526 446 338 225 113 84
BbeI G_GCGC'C
Cuts at: 258 2371 2619 258
Size: 2113 248 2576
Fragments arranged by size:
2576 2113 248
BbsI GAAGACnn'nnnn_
Cuts at: 692 692
Size: 4937
BbvCI CC'TCA_GC
Cuts at: 1292 1796 1292
Size: 504 4433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4903
BciVI GTATCCnnnnn_n'
Cuts at: 2835 3596 4937 2835
Size: 761 1341 2835
Fragments arranged by size:
2835 1341 761
BclI T'GATC_A
Cuts at: 2457 2457
Size: 4937
BglI GCCn_nnn'nGGC
Cuts at: 146 1168 146
Size: 1022 3915
BglII A'GATC_T
Cuts at: 693 2452 4489 693
Size: 1759 2037 1141
Fragments arranged by size:
2037 1759 1141
BlpI GC'TnA_GC
Cuts at: 466 1750 2049 466
Size: 1284 299 3354
Fragments arranged by size:
3354 1284 299
Bme1580I G_kGCm'C
Cuts at: 219 1656 2562 2655 3711 219
Size: 1437 906 93 1056 1445
Fragments arranged by size:
1445 1437 1056 906 93
BmrI ACTGGGnnnn_n'
Cuts at: 1650 2149 2230 2565 4709 1650
Size: 499 81 335 2144 1878
Fragments arranged by size:
2144 1878 499 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3304 3304
Size: 4937
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1036 1435 1036
Size: 399 4538
Bpu10I CC'TnA_GC
Cuts at: 1292 1796 1292
Size: 504 4433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 480 1157 2113 2992 3484 3782 480
Size: 677 956 879 492 298 1635
Fragments arranged by size:
1635 956 879 677 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4641 4641
Size: 4937
BsaAI yAC'GTr
Cuts at: 2290 2290
Size: 4937
BsaBI GATnn'nnATC
Cuts at: 6 2451 4488 6
Size: 2445 2037 455
Fragments arranged by size:
2445 2037 455
BsaHI Gr'CG_yC
Cuts at: 255 344 2368 2616 3318 4814 255
Size: 89 2024 248 702 1496 378
Fragments arranged by size:
2024 1496 702 378 248 89
BsaWI w'CCGG_w
Cuts at: 2126 2647 3325 3599 3746 4157 2126
Size: 521 678 274 147 411 2906
Fragments arranged by size:
2906 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 1309 1636 232
Size: 1077 327 3533
Fragments arranged by size:
3533 1077 327
BseYI C'CCAG_C
Cuts at: 1828 2362 3120 3697 4193 1828
Size: 534 758 577 496 2572
Fragments arranged by size:
2572 758 577 534 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 1874
Size: 4937
BsiEI CG_ry'CG
Cuts at: 104 210 2479 2525 3733 104
Size: 106 2269 46 1208 1308
Fragments arranged by size:
2269 1308 1208 106 46
BsiHKAI G_wGCw'C
Cuts at: 1421 1505 1751 1951 2729 2919 3711 1421
Size: 84 246 200 778 190 792 2647
Fragments arranged by size:
2647 792 778 246 200 190 84
BsmI GAATG_Cn'
Cuts at: 810 1036 1310 1814 810
Size: 226 274 504 3933
Fragments arranged by size:
3933 504 274 226
Bsp1286I G_dGCh'C
Cuts at: 219 332 557 895 1421 1505 1656 1751 1951
Size: 113 225 338 526 84 151 95 200
Cuts at: 1951 2562 2655 2729 2919 2981 3711 219
Size: 611 93 74 190 62 730 1445
Fragments arranged by size:
1445 730 611 526 338 225 200 190
151 113 95 93 84 74 62
BspEI T'CCGG_A
Cuts at: 4157 4157
Size: 4937
BspHI T'CATG_A
Cuts at: 1356 4113 1356
Size: 2757 2180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 261 1006 1987 2201 2503 2884 261
Size: 745 981 214 302 381 2314
Fragments arranged by size:
2314 981 745 381 302 214
BsrBI CCG'CTC
Cuts at: 50 157 308 597 1146 3229 3283 4767 4932
Size: 107 151 289 549 2083 54 1484 165
Cuts at: 4932 50
Size: 55
Fragments arranged by size:
2083 1484 549 289 165 151 107 55
54
BsrDI GCAATG_nn'
Cuts at: 2849 2849
Size: 4937
BsrFI r'CCGG_y
Cuts at: 145 205 2935 3116 3325 145
Size: 60 2730 181 209 1757
Fragments arranged by size:
2730 1757 209 181 60
BssSI C'ACGA_G
Cuts at: 3208 3566 3208
Size: 358 4579
BstAPI GCAn_nnn'nTGC
Cuts at: 366 2314 366
Size: 1948 2989
BstBI TT'CG_AA
Cuts at: 3298 3298
Size: 4937
BstXI CCAn_nnnn'nTGG
Cuts at: 1234 1234
Size: 4937
BstYI r'GATC_y
Cuts at: 1 133 395 693 1962 2429 2452 2787 3033
Size: 132 262 298 1269 467 23 335 246
Cuts at: 3033 4045 4131 4143 4489 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1269 1012 467 449 346 335 298 262
246 132 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1336 1858 1336
Size: 522 4415
BtgI C'CryG_G
Cuts at: 175 210 292 1157 175
Size: 35 82 865 3955
Fragments arranged by size:
3955 865 82 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 837 2887 3068 3231 837
Size: 2050 181 163 2543
Fragments arranged by size:
2543 2050 181 163
BtsI GCAGTG_nn'
Cuts at: 1216 1258 2180 1216
Size: 42 922 3973
Fragments arranged by size:
3973 922 42
DraI TTT'AAA
Cuts at: 1936 1936
Size: 4937
DraIII CAC_nnn'GTG
Cuts at: 1010 1245 1010
Size: 235 4702
DrdI GACnn_nn'nnGTC
Cuts at: 2643 3501 2643
Size: 858 4079
EaeI y'GGCC_r
Cuts at: 143 207 1160 2522 2696 3087 3114 4459 143
Size: 64 953 1362 174 391 27 1345 621
Fragments arranged by size:
1362 1345 953 621 391 174 64 27
EagI C'GGCC_G
Cuts at: 207 2522 207
Size: 2315 2622
EarI CTCTTCn'nnn_
Cuts at: 2960 3170 2960
Size: 210 4727
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1675 3145 3455 3601 4747 1675
Size: 1470 310 146 1146 1865
Fragments arranged by size:
1865 1470 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1419 1503 1949 1419
Size: 84 446 4407
Fragments arranged by size:
4407 446 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 917 1036 1201 1363 1435 1443 2762 3194 3941
Size: 119 165 162 72 8 1319 432 747
Cuts at: 3941 4209 917
Size: 268 1645
Fragments arranged by size:
1645 1319 747 432 268 165 162 119
72 8
EcoNI CCTnn'n_nnAGG
Cuts at: 954 1795 954
Size: 841 4096
EcoO109I rG'GnC_Cy
Cuts at: 215 216 273 591 959 995 1272 2076 215
Size: 1 57 318 368 36 277 804 3076
Fragments arranged by size:
3076 804 368 318 277 57 36 1
EcoRI G'AATT_C
Cuts at: 1408 1941 1408
Size: 533 4404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1504 2956 1504
Size: 1452 3485
FspI TGC'GCA
Cuts at: 2718 2718
Size: 4937
HaeII r_GCGC'y
Cuts at: 258 2371 2619 3641 4620 258
Size: 2113 248 1022 979 575
Fragments arranged by size:
2113 1022 979 575 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 292 324 387 419 544 576 1386 1418 2779
Size: 32 63 32 125 32 810 32 1361
Cuts at: 2779 2809 2811 2841 292
Size: 30 2 30 2388
Fragments arranged by size:
2388 1361 810 125 63 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1976 1976
Size: 4937
HindIII A'AGCT_T
Cuts at: 713 1210 713
Size: 497 4440
KasI G'GCGC_C
Cuts at: 254 2367 2615 254
Size: 2113 248 2576
Fragments arranged by size:
2576 2113 248
KpnI G_GTAC'C
Cuts at: 1957 1957
Size: 4937
MluI A'CGCG_T
Cuts at: 4370 4370
Size: 4937
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3608 3792 3608
Size: 184 4753
MscI TGG'CCA
Cuts at: 2698 2698
Size: 4937
MslI CAynn'nnrTG
Cuts at: 2926 3053 2926
Size: 127 4810
MspA1I CmG'CkG
Cuts at: 177 212 294 2048 2362 2722 3735 3980 177
Size: 35 82 1754 314 360 1013 245 1134
Fragments arranged by size:
1754 1134 1013 360 314 245 82 35
NaeI GCC'GGC
Cuts at: 147 207 3118 147
Size: 60 2911 1966
Fragments arranged by size:
2911 1966 60
NarI GG'CG_CC
Cuts at: 255 2368 2616 255
Size: 2113 248 2576
Fragments arranged by size:
2576 2113 248
NdeI CA'TA_TG
Cuts at: 1908 1908
Size: 4937
NgoMIV G'CCGG_C
Cuts at: 145 205 3116 145
Size: 60 2911 1966
Fragments arranged by size:
2911 1966 60
NspI r_CATG'y
Cuts at: 1990 2490 3397 1990
Size: 500 907 3530
Fragments arranged by size:
3530 907 500
PciI A'CATG_T
Cuts at: 3393 3393
Size: 4937
PflMI CCAn_nnn'nTGG
Cuts at: 1059 1059
Size: 4937
PfoI T'CCnGG_A
Cuts at: 1412 1412
Size: 4937
PmeI GTTT'AAAC
Cuts at: 1936 1936
Size: 4937
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 4076
Size: 32 4905
PpuMI rG'GwC_Cy
Cuts at: 273 591 959 995 1272 273
Size: 318 368 36 277 3938
Fragments arranged by size:
3938 368 318 277 36
PsiI TTA'TAA
Cuts at: 1516 1516
Size: 4937
PspOMI G'GGCC_C
Cuts at: 215 215
Size: 4937
PstI C_TGCA'G
Cuts at: 1305 1871 1984 2669 1305
Size: 566 113 685 3573
Fragments arranged by size:
3573 685 566 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4937
PvuII CAG'CTG
Cuts at: 2362 2722 2362
Size: 360 4577
RsrII CG'GwC_CG
Cuts at: 3132 3132
Size: 4937
SacI G_AGCT'C
Cuts at: 1421 1505 1951 1421
Size: 84 446 4407
Fragments arranged by size:
4407 446 84
SacII CC_GC'GG
Cuts at: 178 213 295 178
Size: 35 82 4820
Fragments arranged by size:
4820 82 35
SalI G'TCGA_C
Cuts at: 1974 1974
Size: 4937
SanDI GG'GwC_CC
Cuts at: 1272 1272
Size: 4937
SapI GCTCTTCn'nnn_
Cuts at: 2960 3170 2960
Size: 210 4727
SbfI CC_TGCA'GG
Cuts at: 1984 1984
Size: 4937
ScaI AGT'ACT
Cuts at: 4454 4454
Size: 4937
SfcI C'TryA_G
Cuts at: 33 399 1301 1867 1980 2665 3658 3849 33
Size: 366 902 566 113 685 993 191 1121
Fragments arranged by size:
1121 993 902 685 566 366 191 113
SfoI GGC'GCC
Cuts at: 256 2369 2617 256
Size: 2113 248 2576
Fragments arranged by size:
2576 2113 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4937
SmaI CCC'GGG
Cuts at: 1959 1959
Size: 4937
SmlI C'TyrA_G
Cuts at: 152 495 1172 2092 3007 3499 3761 152
Size: 343 677 920 915 492 262 1328
Fragments arranged by size:
1328 920 915 677 492 343 262
SpeI A'CTAG_T
Cuts at: 67 163 67
Size: 96 4841
SphI G_CATG'C
Cuts at: 1990 2490 1990
Size: 500 4437
StyI C'CwwG_G
Cuts at: 687 2071 2425 687
Size: 1384 354 3199
Fragments arranged by size:
3199 1384 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2520 2653 3112 3321 2520
Size: 133 459 209 4136
Fragments arranged by size:
4136 459 209 133
TatI w'GTAC_w
Cuts at: 786 1733 4452 786
Size: 947 2719 1271
Fragments arranged by size:
2719 1271 947
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1208 1229 1240 1261 4454 4486 1208
Size: 21 11 21 3193 32 1659
Fragments arranged by size:
3193 1659 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 2734 2734
Size: 4937
XbaI T'CTAG_A
Cuts at: 1968 1968
Size: 4937
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1164 2432 1164
Size: 1268 3669
XhoI C'TCGA_G
Cuts at: 152 152
Size: 4937
XmaI C'CCGG_G
Cuts at: 1957 1957
Size: 4937
XmnI GAAnn'nnTTC
Cuts at: 643 1484 643
Size: 841 4096
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
BaeI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI
HpaI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PspXI PsrI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI