Restriction map for
pF1KE0305
(Circular) (Six-base) MAPSORT of: pF1KE0305.seq Check: 524 from: 1 to: 5069
pF1KE0305 5069 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1783 1783
Size: 5069
AccI GT'mk_AC
Cuts at: 1659 2107 1659
Size: 448 4621
Acc65I G'GTAC_C
Cuts at: 1106 2085 1106
Size: 979 4090
AclI AA'CG_TT
Cuts at: 4909 4909
Size: 5069
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 460 2894 3326 4073 4341 432
Size: 28 2434 432 747 268 1160
Fragments arranged by size:
2434 1160 747 432 268 28
AfeI AGC'GCT
Cuts at: 243 526 243
Size: 283 4786
AflIII A'CryG_T
Cuts at: 1085 2421 3525 4502 1085
Size: 1336 1104 977 1652
Fragments arranged by size:
1652 1336 1104 977
AgeI A'CCGG_T
Cuts at: 3457 3457
Size: 5069
AhdI GACnn_n'nnGTC
Cuts at: 809 809
Size: 5069
AleI CACnn'nnGTG
Cuts at: 1876 1876
Size: 5069
AlwNI CAG_nnn'CTG
Cuts at: 345 2374 3941 345
Size: 2029 1567 1473
Fragments arranged by size:
2029 1567 1473
ApaI G_GGCC'C
Cuts at: 598 694 871 1408 1417 1547 1624 598
Size: 96 177 537 9 130 77 4043
Fragments arranged by size:
4043 537 177 130 96 77 9
ApaLI G'TGCA_C
Cuts at: 3839 3839
Size: 5069
ApoI r'AATT_y
Cuts at: 2073 4422 4678 2073
Size: 2349 256 2464
Fragments arranged by size:
2464 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5069
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5069
AvaI C'yCGr_G
Cuts at: 160 228 864 1119 1407 1624 2089 160
Size: 68 636 255 288 217 465 3140
Fragments arranged by size:
3140 636 465 288 255 217 68
AvrII C'CTAG_G
Cuts at: 1866 1866
Size: 5069
BamHI G'GATC_C
Cuts at: 2094 2094
Size: 5069
BanI G'GyrC_C
Cuts at: 1106 1630 1870 2085 2499 2747 2782 1106
Size: 524 240 215 414 248 35 3393
Fragments arranged by size:
3393 524 414 248 240 215 35
BanII G_rGCy'C
Cuts at: 398 503 598 610 694 871 1408 1417 1547
Size: 105 95 12 84 177 537 9 130
Cuts at: 1547 1624 1748 2083 3113 398
Size: 77 124 335 1030 2354
Fragments arranged by size:
2354 1030 537 335 177 130 124 105
95 84 77 12 9
BbeI G_GCGC'C
Cuts at: 2503 2751 2503
Size: 248 4821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1004 1038 1004
Size: 34 5035
BciVI GTATCCnnnnn_n'
Cuts at: 2967 3728 5069 2967
Size: 761 1341 2967
Fragments arranged by size:
2967 1341 761
BclI T'GATC_A
Cuts at: 2589 2589
Size: 5069
BglI GCCn_nnn'nGGC
Cuts at: 367 766 1412 1475 1628 367
Size: 399 646 63 153 3808
Fragments arranged by size:
3808 646 399 153 63
BglII A'GATC_T
Cuts at: 2584 4621 2584
Size: 2037 3032
BlpI GC'TnA_GC
Cuts at: 238 2181 238
Size: 1943 3126
Bme1580I G_kGCm'C
Cuts at: 598 694 755 871 1173 1408 1417 1547 1592
Size: 96 61 116 302 235 9 130 45
Cuts at: 1592 1624 1633 2694 2787 3843 598
Size: 32 9 1061 93 1056 1824
Fragments arranged by size:
1824 1061 1056 302 235 130 116 96
93 61 45 32 9 9
BmgBI CAC'GTC
Cuts at: 321 1143 1389 321
Size: 822 246 4001
Fragments arranged by size:
4001 822 246
BmrI ACTGGGnnnn_n'
Cuts at: 864 1712 2281 2362 2697 4841 864
Size: 848 569 81 335 2144 1092
Fragments arranged by size:
2144 1092 848 569 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3436 3436
Size: 5069
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 1014 1262 1758 382
Size: 632 248 496 3693
Fragments arranged by size:
3693 632 496 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1621 1687 2245 3124 3616 3914 1621
Size: 66 558 879 492 298 2776
Fragments arranged by size:
2776 879 558 492 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 1506 4773 1506
Size: 3267 1802
BsaAI yAC'GTr
Cuts at: 1055 1088 2422 1055
Size: 33 1334 3702
Fragments arranged by size:
3702 1334 33
BsaBI GATnn'nnATC
Cuts at: 6 2583 4620 6
Size: 2577 2037 455
Fragments arranged by size:
2577 2037 455
BsaHI Gr'CG_yC
Cuts at: 1679 1733 2500 2748 3450 4946 1679
Size: 54 767 248 702 1496 1802
Fragments arranged by size:
1802 1496 767 702 248 54
BsaWI w'CCGG_w
Cuts at: 503 2258 2779 3457 3731 3878 4289 503
Size: 1755 521 678 274 147 411 1283
Fragments arranged by size:
1755 1283 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1975 2005 2020 2050 1975
Size: 30 15 30 4994
Fragments arranged by size:
4994 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 322 1357 1570 1794 1963 322
Size: 1035 213 224 169 3428
Fragments arranged by size:
3428 1035 224 213 169
BseYI C'CCAG_C
Cuts at: 155 314 338 452 554 568 1214 1478 1747
Size: 159 24 114 102 14 646 264 269
Cuts at: 1747 2494 3252 3829 4325 155
Size: 747 758 577 496 899
Fragments arranged by size:
899 758 747 646 577 496 269 264
159 114 102 24 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1290 1290
Size: 5069
BsiEI CG_ry'CG
Cuts at: 104 2611 2657 3865 104
Size: 2507 46 1208 1308
Fragments arranged by size:
2507 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 503 1278 1585 2083 2861 3051 3843 503
Size: 775 307 498 778 190 792 1729
Fragments arranged by size:
1729 792 778 775 498 307 190
BsmI GAATG_Cn'
Cuts at: 1156 1216 1980 1156
Size: 60 764 4245
Fragments arranged by size:
4245 764 60
Bsp1286I G_dGCh'C
Cuts at: 398 503 598 610 694 755 871 1173 1278
Size: 105 95 12 84 61 116 302 105
Cuts at: 1278 1408 1417 1547 1585 1592 1624 1633 1748
Size: 130 9 130 38 7 32 9 115
Cuts at: 1748 2083 2694 2787 2861 3051 3113 3843 398
Size: 335 611 93 74 190 62 730 1624
Fragments arranged by size:
1624 730 611 335 302 190 130 130
116 115 105 105 95 93 84 74
62 61 38 32 12 9 9 7
BspEI T'CCGG_A
Cuts at: 503 4289 503
Size: 3786 1283
BspHI T'CATG_A
Cuts at: 4245 4245
Size: 5069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1783 2119 2333 2635 3016 1783
Size: 336 214 302 381 3836
Fragments arranged by size:
3836 381 336 302 214
BsrBI CCG'CTC
Cuts at: 50 561 3361 3415 4899 5064 50
Size: 511 2800 54 1484 165 55
Fragments arranged by size:
2800 1484 511 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2981 2981
Size: 5069
BsrFI r'CCGG_y
Cuts at: 671 3067 3248 3457 671
Size: 2396 181 209 2283
Fragments arranged by size:
2396 2283 209 181
BssSI C'ACGA_G
Cuts at: 510 3340 3698 510
Size: 2830 358 1881
Fragments arranged by size:
2830 1881 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1848 2446 1848
Size: 598 4471
BstBI TT'CG_AA
Cuts at: 3430 3430
Size: 5069
BstEII G'GTnAC_C
Cuts at: 702 999 702
Size: 297 4772
BstXI CCAn_nnnn'nTGG
Cuts at: 120 1736 120
Size: 1616 3453
BstYI r'GATC_y
Cuts at: 1 2094 2561 2584 2919 3165 4177 4263 4275
Size: 2093 467 23 335 246 1012 86 12
Cuts at: 4275 4621 1
Size: 346 449
Fragments arranged by size:
2093 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 346 346
Size: 5069
BtgI C'CryG_G
Cuts at: 107 119 1071 1511 1808 1874 2024 107
Size: 12 952 440 297 66 150 3152
Fragments arranged by size:
3152 952 440 297 150 66 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3019 3200 3363 3019
Size: 181 163 4725
Fragments arranged by size:
4725 181 163
BtsI GCAGTG_nn'
Cuts at: 261 1329 2312 261
Size: 1068 983 3018
Fragments arranged by size:
3018 1068 983
DraI TTT'AAA
Cuts at: 2068 2068
Size: 5069
DrdI GACnn_nn'nnGTC
Cuts at: 2775 3633 2775
Size: 858 4211
EaeI y'GGCC_r
Cuts at: 110 287 767 1358 1811 1880 2654 2828 3219
Size: 177 480 591 453 69 774 174 391
Cuts at: 3219 3246 4591 110
Size: 27 1345 588
Fragments arranged by size:
1345 774 591 588 480 453 391 177
174 69 27
EagI C'GGCC_G
Cuts at: 2654 2654
Size: 5069
EarI CTCTTCn'nnn_
Cuts at: 292 1936 3092 3302 292
Size: 1644 1156 210 2059
Fragments arranged by size:
2059 1644 1156 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3277 3587 3733 4879 3277
Size: 310 146 1146 3467
Fragments arranged by size:
3467 1146 310 146
EcoICRI GAG'CTC
Cuts at: 501 2081 501
Size: 1580 3489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 432 460 1014 1262 1758 2894 3326 4073
Size: 50 28 554 248 496 1136 432 747
Cuts at: 4073 4341 382
Size: 268 1110
Fragments arranged by size:
1136 1110 747 554 496 432 268 248
50 28
EcoNI CCTnn'n_nnAGG
Cuts at: 826 826
Size: 5069
EcoO109I rG'GnC_Cy
Cuts at: 594 595 690 837 868 1066 1250 1413 1414
Size: 1 95 147 31 198 184 163 1
Cuts at: 1414 1451 1516 1544 1620 1621 1724 2208 594
Size: 37 65 28 76 1 103 484 3455
Fragments arranged by size:
3455 484 198 184 163 147 103 95
76 65 37 31 28 1 1 1
EcoRI G'AATT_C
Cuts at: 2073 2073
Size: 5069
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 672 1679 3088 672
Size: 1007 1409 2653
Fragments arranged by size:
2653 1409 1007
FspI TGC'GCA
Cuts at: 2850 2850
Size: 5069
HaeII r_GCGC'y
Cuts at: 245 528 2503 2751 3773 4752 245
Size: 283 1975 248 1022 979 562
Fragments arranged by size:
1975 1022 979 562 283 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 795 827 1975 2007 2911 2941 2943 2973 795
Size: 32 1148 32 904 30 2 30 2891
Fragments arranged by size:
2891 1148 904 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1185 2108 1185
Size: 923 4146
KasI G'GCGC_C
Cuts at: 2499 2747 2499
Size: 248 4821
KpnI G_GTAC'C
Cuts at: 1110 2089 1110
Size: 979 4090
MluI A'CGCG_T
Cuts at: 4502 4502
Size: 5069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2068 3740 3924 2068
Size: 1672 184 3213
Fragments arranged by size:
3213 1672 184
MscI TGG'CCA
Cuts at: 112 289 1813 1882 2830 112
Size: 177 1524 69 948 2351
Fragments arranged by size:
2351 1524 948 177 69
MslI CAynn'nnrTG
Cuts at: 118 1876 3058 3185 118
Size: 1758 1182 127 2002
Fragments arranged by size:
2002 1758 1182 127
MspA1I CmG'CkG
Cuts at: 438 452 1094 1113 1128 1205 1768 2180 2494
Size: 14 642 19 15 77 563 412 314
Cuts at: 2494 2854 3867 4112 438
Size: 360 1013 245 1395
Fragments arranged by size:
1395 1013 642 563 412 360 314 245
77 19 15 14
NaeI GCC'GGC
Cuts at: 673 3250 673
Size: 2577 2492
NarI GG'CG_CC
Cuts at: 2500 2748 2500
Size: 248 4821
NcoI C'CATG_G
Cuts at: 107 119 1071 1511 2024 107
Size: 12 952 440 513 3152
Fragments arranged by size:
3152 952 513 440 12
NgoMIV G'CCGG_C
Cuts at: 671 3248 671
Size: 2577 2492
NspI r_CATG'y
Cuts at: 669 2122 2622 3529 669
Size: 1453 500 907 2209
Fragments arranged by size:
2209 1453 907 500
PasI CC'CwG_GG
Cuts at: 684 1192 1299 684
Size: 508 107 4454
Fragments arranged by size:
4454 508 107
PciI A'CATG_T
Cuts at: 3525 3525
Size: 5069
PflMI CCAn_nnn'nTGG
Cuts at: 1042 1389 1540 1042
Size: 347 151 4571
Fragments arranged by size:
4571 347 151
PfoI T'CCnGG_A
Cuts at: 1239 1239
Size: 5069
PmeI GTTT'AAAC
Cuts at: 2068 2068
Size: 5069
PmlI CAC'GTG
Cuts at: 1055 1088 1055
Size: 33 5036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 4208
Size: 32 5037
PpuMI rG'GwC_Cy
Cuts at: 1066 1451 1724 1066
Size: 385 273 4411
Fragments arranged by size:
4411 385 273
PshAI GACnn'nnGTC
Cuts at: 428 1121 428
Size: 693 4376
PspOMI G'GGCC_C
Cuts at: 594 690 867 1404 1413 1543 1620 594
Size: 96 177 537 9 130 77 4043
Fragments arranged by size:
4043 537 177 130 96 77 9
PspXI vC'TCGA_Gb
Cuts at: 1119 1119
Size: 5069
PstI C_TGCA'G
Cuts at: 1794 2116 2801 1794
Size: 322 685 4062
Fragments arranged by size:
4062 685 322
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5069
PvuII CAG'CTG
Cuts at: 452 1113 1128 1768 2494 2854 452
Size: 661 15 640 726 360 2667
Fragments arranged by size:
2667 726 661 640 360 15
RsrII CG'GwC_CG
Cuts at: 3264 3264
Size: 5069
SacI G_AGCT'C
Cuts at: 503 2083 503
Size: 1580 3489
SalI G'TCGA_C
Cuts at: 2106 2106
Size: 5069
SanDI GG'GwC_CC
Cuts at: 1066 1724 1066
Size: 658 4411
SapI GCTCTTCn'nnn_
Cuts at: 292 3092 3302 292
Size: 2800 210 2059
Fragments arranged by size:
2800 2059 210
SbfI CC_TGCA'GG
Cuts at: 1794 2116 1794
Size: 322 4747
ScaI AGT'ACT
Cuts at: 332 4586 332
Size: 4254 815
SexAI A'CCwGG_T
Cuts at: 995 995
Size: 5069
SfcI C'TryA_G
Cuts at: 33 1790 2112 2797 3790 3981 33
Size: 1757 322 685 993 191 1121
Fragments arranged by size:
1757 1121 993 685 322 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1412 1412
Size: 5069
SfoI GGC'GCC
Cuts at: 2501 2749 2501
Size: 248 4821
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5069
SmaI CCC'GGG
Cuts at: 162 1409 2091 162
Size: 1247 682 3140
Fragments arranged by size:
3140 1247 682
SmlI C'TyrA_G
Cuts at: 1119 1636 1702 2224 3139 3631 3893 1119
Size: 517 66 522 915 492 262 2295
Fragments arranged by size:
2295 915 522 517 492 262 66
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5069
SphI G_CATG'C
Cuts at: 2122 2622 2122
Size: 500 4569
StyI C'CwwG_G
Cuts at: 107 119 740 1071 1101 1511 1866 2024 2203
Size: 12 621 331 30 410 355 158 179
Cuts at: 2203 2557 107
Size: 354 2619
Fragments arranged by size:
2619 621 410 355 354 331 179 158
30 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 444 1470 1662 2652 2785 3244 3453 444
Size: 1026 192 990 133 459 209 2060
Fragments arranged by size:
2060 1026 990 459 209 192 133
TatI w'GTAC_w
Cuts at: 330 2036 4584 330
Size: 1706 2548 815
Fragments arranged by size:
2548 1706 815
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4586 4618 4586
Size: 32 5037
Tth111I GACn'n_nGTC
Cuts at: 2866 2866
Size: 5069
XbaI T'CTAG_A
Cuts at: 2100 2100
Size: 5069
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 738 1059 2564 738
Size: 321 1505 3243
Fragments arranged by size:
3243 1505 321
XhoI C'TCGA_G
Cuts at: 1119 1119
Size: 5069
XmaI C'CCGG_G
Cuts at: 160 1407 2089 160
Size: 1247 682 3140
Fragments arranged by size:
3140 1247 682
XmnI GAAnn'nnTTC
Cuts at: 445 445
Size: 5069
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AlfI AloI AscI BaeI BbsI BbvCI
BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PsiI
PsrI SacII SgrAI SnaBI SrfI SspI StuI SwaI
ZraI