Restriction map for
pF1KE0298
(Circular) (Six-base) MAPSORT of: pF1KE0298.seq Check: 496 from: 1 to: 11267
pF1KE0298 11267 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5739 7960 7968 5739
Size: 2221 8 9038
Fragments arranged by size:
9038 2221 8
AccI GT'mk_AC
Cuts at: 3433 4708 5304 6983 8305 3433
Size: 1275 596 1679 1322 6395
Fragments arranged by size:
6395 1679 1322 1275 596
Acc65I G'GTAC_C
Cuts at: 7326 8283 7326
Size: 957 10310
AclI AA'CG_TT
Cuts at: 4613 11107 4613
Size: 6494 4773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 488 503 1879 2807 3220 3265 3293 3709
Size: 184 15 1376 928 413 45 28 416
Cuts at: 3709 4796 5312 5723 5978 6757 7348 7522 8122
Size: 1087 516 411 255 779 591 174 600
Cuts at: 8122 8135 8196 9092 9524 9877 10539 304
Size: 13 61 896 432 353 662 1032
Fragments arranged by size:
1376 1087 1032 928 896 779 662 600
591 516 432 416 413 411 353 255
184 174 61 45 28 15 13
AflII C'TTAA_G
Cuts at: 1824 5949 6174 1824
Size: 4125 225 6917
Fragments arranged by size:
6917 4125 225
AflIII A'CryG_T
Cuts at: 1928 3759 8619 10419 10700 1928
Size: 1831 4860 1800 281 2495
Fragments arranged by size:
4860 2495 1831 1800 281
AleI CACnn'nnGTG
Cuts at: 7354 7447 7354
Size: 93 11174
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1529 6965 7513 1529
Size: 5436 548 5283
Fragments arranged by size:
5436 5283 548
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1821 1853 2502 2534 7661 7693 1821
Size: 32 649 32 5127 32 5395
Fragments arranged by size:
5395 5127 649 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1516 2659 2862 3600 5898 7680 8572 10010 1516
Size: 1143 203 738 2298 1782 892 1438 2773
Fragments arranged by size:
2773 2298 1782 1438 1143 892 738 203
ApaI G_GGCC'C
Cuts at: 3406 3406
Size: 11267
ApaLI G'TGCA_C
Cuts at: 1654 1874 8206 10105 1654
Size: 220 6332 1899 2816
Fragments arranged by size:
6332 2816 1899 220
ApoI r'AATT_y
Cuts at: 125 571 1968 3024 3043 4734 5157 5215 5253
Size: 446 1397 1056 19 1691 423 58 38
Cuts at: 5253 5641 5677 6217 6267 7040 8271 10620 10876
Size: 388 36 540 50 773 1231 2349 256
Cuts at: 10876 125
Size: 516
Fragments arranged by size:
2349 1691 1397 1231 1056 773 540 516
446 423 388 256 58 50 38 36
19
AseI AT'TA_AT
Cuts at: 20 4220 4921 20
Size: 4200 701 6366
Fragments arranged by size:
6366 4200 701
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11267
AvaI C'yCGr_G
Cuts at: 3668 4340 8287 3668
Size: 672 3947 6648
Fragments arranged by size:
6648 3947 672
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7533 7566 7533
Size: 33 11234
BamHI G'GATC_C
Cuts at: 1851 3420 7316 8292 1851
Size: 1569 3896 976 4826
Fragments arranged by size:
4826 3896 1569 976
BanI G'GyrC_C
Cuts at: 1603 1633 4995 7326 8283 8697 8945 8980 1603
Size: 30 3362 2331 957 414 248 35 3890
Fragments arranged by size:
3890 3362 2331 957 414 248 35 30
BanII G_rGCy'C
Cuts at: 751 3406 4967 5528 7090 7812 8281 9311 751
Size: 2655 1561 561 1562 722 469 1030 2707
Fragments arranged by size:
2707 2655 1562 1561 1030 722 561 469
BbeI G_GCGC'C
Cuts at: 1607 1637 8701 8949 1607
Size: 30 7064 248 3925
Fragments arranged by size:
7064 3925 248 30
BbsI GAAGACnn'nnnn_
Cuts at: 869 1583 2822 4745 6686 6922 869
Size: 714 1239 1923 1941 236 5214
Fragments arranged by size:
5214 1941 1923 1239 714 236
BbvCI CC'TCA_GC
Cuts at: 4508 4508
Size: 11267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 246 280 4251 4285 90
Size: 34 122 34 3971 34 7072
Fragments arranged by size:
7072 3971 122 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6573 9165 10221 11267 6573
Size: 2592 1056 1046 6573
Fragments arranged by size:
6573 2592 1056 1046
BclI T'GATC_A
Cuts at: 3864 8787 3864
Size: 4923 6344
BfrBI ATG'CAT
Cuts at: 644 644
Size: 11267
BglI GCCn_nnn'nGGC
Cuts at: 7720 7720
Size: 11267
BglII A'GATC_T
Cuts at: 1132 1756 5094 7742 8782 10819 1132
Size: 624 3338 2648 1040 2037 1580
Fragments arranged by size:
3338 2648 2037 1580 1040 624
BlpI GC'TnA_GC
Cuts at: 7121 8379 7121
Size: 1258 10009
Bme1580I G_kGCm'C
Cuts at: 1658 1878 2771 3116 3406 7361 8210 8892 8985
Size: 220 893 345 290 3955 849 682 93
Cuts at: 8985 10109 1658
Size: 1124 2816
Fragments arranged by size:
3955 2816 1124 893 849 682 345 290
220 93
BmgBI CAC'GTC
Cuts at: 5436 8087 8220 5436
Size: 2651 133 8483
Fragments arranged by size:
8483 2651 133
BmrI ACTGGGnnnn_n'
Cuts at: 1501 3305 3577 4364 4472 8479 8560 8895 11039
Size: 1804 272 787 108 4007 81 335 2144
Cuts at: 11039 1501
Size: 1729
Fragments arranged by size:
4007 2144 1804 1729 787 335 272 108
81
BmtI G_CTAG'C
Cuts at: 6743 6743
Size: 11267
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 332 9634 332
Size: 9302 1965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1415 1932 3720 3999 4584 5783 7202 7493 7723
Size: 517 1788 279 585 1199 1419 291 230
Cuts at: 7723 7796 8138 1415
Size: 73 342 4544
Fragments arranged by size:
4544 1788 1419 1199 585 517 342 291
279 230 73
Bpu10I CC'TnA_GC
Cuts at: 3098 3407 3532 3560 4508 4966 6886 3098
Size: 309 125 28 948 458 1920 7479
Fragments arranged by size:
7479 1920 948 458 309 125 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 585 2359 3762 7166 8443 9322 10036 10334 585
Size: 1774 1403 3404 1277 879 714 298 1518
Fragments arranged by size:
3404 1774 1518 1403 1277 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3629 3683 3836 4251 5433 7741 10971 3629
Size: 54 153 415 1182 2308 3230 3925
Fragments arranged by size:
3925 3230 2308 1182 415 153 54
BsaAI yAC'GTr
Cuts at: 2022 8037 8620 2022
Size: 6015 583 4669
Fragments arranged by size:
6015 4669 583
BsaBI GATnn'nnATC
Cuts at: 6 546 3640 4430 8781 10818 6
Size: 540 3094 790 4351 2037 455
Fragments arranged by size:
4351 3094 2037 790 540 455
BsaHI Gr'CG_yC
Cuts at: 1604 1634 3181 6551 8698 8946 9648 11144 1604
Size: 30 1547 3370 2147 248 702 1496 1727
Fragments arranged by size:
3370 2147 1727 1547 1496 702 248 30
BsaWI w'CCGG_w
Cuts at: 8456 8977 9655 10066 10213 10487 8456
Size: 521 678 411 147 274 9236
Fragments arranged by size:
9236 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2502 2532 3511 3541 4011 4041 6053 6083 6909
Size: 30 979 30 470 30 2012 30 826
Cuts at: 6909 6939 7887 7917 8136 8166 2502
Size: 30 948 30 219 30 5603
Fragments arranged by size:
5603 2012 979 948 826 470 219 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1136 1609 2356 2666 2669 2928 3731 7895 1136
Size: 473 747 310 3 259 803 4164 4508
Fragments arranged by size:
4508 4164 803 747 473 310 259 3
BseYI C'CCAG_C
Cuts at: 2039 2197 3154 3896 5355 5891 7271 8692 9450
Size: 158 957 742 1459 536 1380 1421 758
Cuts at: 9450 10115 10523 2039
Size: 665 408 2783
Fragments arranged by size:
2783 1459 1421 1380 957 758 742 665
536 408 158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 779 5008 6444 7399 779
Size: 4229 1436 955 4647
Fragments arranged by size:
4647 4229 1436 955
BsiEI CG_ry'CG
Cuts at: 104 8809 8855 10085 104
Size: 8705 46 1230 1286
Fragments arranged by size:
8705 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 733 1658 1878 3104 4687 4967 6892 7127 7812
Size: 925 220 1226 1583 280 1925 235 685
Cuts at: 7812 8210 8281 9059 9249 10109 733
Size: 398 71 778 190 860 1891
Fragments arranged by size:
1925 1891 1583 1226 925 860 778 685
398 280 235 220 190 71
BsmI GAATG_Cn'
Cuts at: 646 691 1068 3297 4491 4842 5196 7266 7665
Size: 45 377 2229 1194 351 354 2070 399
Cuts at: 7665 646
Size: 4248
Fragments arranged by size:
4248 2229 2070 1194 399 377 354 351
45
BsmBI CGTCTCn'nnnn_
Cuts at: 6558 6558
Size: 11267
Bsp1286I G_dGCh'C
Cuts at: 733 751 1658 1878 2771 3104 3116 3406 4687
Size: 18 907 220 893 333 12 290 1281
Cuts at: 4687 4967 5528 6892 7090 7127 7361 7812 8210
Size: 280 561 1364 198 37 234 451 398
Cuts at: 8210 8281 8892 8985 9059 9249 9311 10109 733
Size: 71 611 93 74 190 62 798 1891
Fragments arranged by size:
1891 1364 1281 907 893 798 611 561
451 398 333 290 280 234 220 198
190 93 74 71 62 37 18 12
BspHI T'CATG_A
Cuts at: 9699 9699
Size: 11267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2667 5739 7338 7665 7960 7968 8317 8531 8833
Size: 3072 1599 327 295 8 349 214 302
Cuts at: 8833 9214 2667
Size: 381 4720
Fragments arranged by size:
4720 3072 1599 381 349 327 302 295
214 8
BsrBI CCG'CTC
Cuts at: 50 1385 2346 9559 9613 11097 11262 50
Size: 1335 961 7213 54 1484 165 55
Fragments arranged by size:
7213 1484 1335 961 165 55 54
BsrDI GCAATG_nn'
Cuts at: 719 941 2290 5372 7756 9179 719
Size: 222 1349 3082 2384 1423 2807
Fragments arranged by size:
3082 2807 2384 1423 1349 222
BsrFI r'CCGG_y
Cuts at: 9265 9446 9265
Size: 181 11086
BsrGI T'GTAC_A
Cuts at: 4443 5770 4443
Size: 1327 9940
BssSI C'ACGA_G
Cuts at: 1537 9538 10246 1537
Size: 8001 708 2558
Fragments arranged by size:
8001 2558 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1653 2659 4848 4950 4995 7841 8644 1653
Size: 1006 2189 102 45 2846 803 4276
Fragments arranged by size:
4276 2846 2189 1006 803 102 45
BstBI TT'CG_AA
Cuts at: 5721 9628 5721
Size: 3907 7360
BstEII G'GTnAC_C
Cuts at: 2192 7913 7972 2192
Size: 5721 59 5487
Fragments arranged by size:
5721 5487 59
BstXI CCAn_nnnn'nTGG
Cuts at: 2893 2893
Size: 11267
BstYI r'GATC_y
Cuts at: 1 547 1132 1756 1851 3420 4575 4869 5094
Size: 546 585 624 95 1569 1155 294 225
Cuts at: 5094 7316 7742 8292 8759 8782 9117 9363 9669
Size: 2222 426 550 467 23 335 246 306
Cuts at: 9669 9681 9767 10819 1
Size: 12 86 1052 449
Fragments arranged by size:
2222 1569 1155 1052 624 585 550 546
467 449 426 335 306 294 246 225
95 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 7718 7907 8133 7718
Size: 189 226 10852
Fragments arranged by size:
10852 226 189
BtgI C'CryG_G
Cuts at: 1637 1847 2413 3416 5378 8188 1637
Size: 210 566 1003 1962 2810 4716
Fragments arranged by size:
4716 2810 1962 1003 566 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 8243 9217 9398 9561 8243
Size: 974 181 163 9949
Fragments arranged by size:
9949 974 181 163
BtsI GCAGTG_nn'
Cuts at: 686 1100 4496 6456 7497 8510 686
Size: 414 3396 1960 1041 1013 3443
Fragments arranged by size:
3443 3396 1960 1041 1013 414
ClaI AT'CG_AT
Cuts at: 4115 4115
Size: 11267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 6026 6039 6061 6074 6195 6230 6026
Size: 13 22 13 121 35 11063
Fragments arranged by size:
11063 121 35 22 13 13
DraI TTT'AAA
Cuts at: 1373 2472 2495 4412 8266 1373
Size: 1099 23 1917 3854 4374
Fragments arranged by size:
4374 3854 1917 1099 23
DraIII CAC_nnn'GTG
Cuts at: 757 757
Size: 11267
DrdI GACnn_nn'nnGTC
Cuts at: 6145 6799 8973 10317 6145
Size: 654 2174 1344 7095
Fragments arranged by size:
7095 2174 1344 654
EaeI y'GGCC_r
Cuts at: 2324 7515 8852 9026 9417 9444 10789 2324
Size: 5191 1337 174 391 27 1345 2802
Fragments arranged by size:
5191 2802 1345 1337 391 174 27
EagI C'GGCC_G
Cuts at: 8852 8852
Size: 11267
EarI CTCTTCn'nnn_
Cuts at: 323 341 654 2826 4478 4833 6326 6354 9290
Size: 18 313 2172 1652 355 1493 28 2936
Cuts at: 9290 9500 323
Size: 210 2090
Fragments arranged by size:
2936 2172 2090 1652 1493 355 313 210
28 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6436 9475 10217 10363 11077 6436
Size: 3039 742 146 714 6626
Fragments arranged by size:
6626 3039 742 714 146
EcoICRI GAG'CTC
Cuts at: 4965 7810 8279 4965
Size: 2845 469 7953
Fragments arranged by size:
7953 2845 469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 488 503 1415 1879 1932 2807 3220 3265
Size: 184 15 912 464 53 875 413 45
Cuts at: 3265 3293 3709 3720 3999 4584 4796 5312 5723
Size: 28 416 11 279 585 212 516 411
Cuts at: 5723 5783 5978 6757 7202 7348 7493 7522 7723
Size: 60 195 779 445 146 145 29 201
Cuts at: 7723 7796 8122 8135 8138 8196 9092 9524 9877
Size: 73 326 13 3 58 896 432 353
Cuts at: 9877 10539 304
Size: 662 1032
Fragments arranged by size:
1032 912 896 875 779 662 585 516
464 445 432 416 413 411 353 326
279 212 201 195 184 146 145 73
60 58 53 45 29 28 15 13
11 3
EcoO109I rG'GnC_Cy
Cuts at: 1890 2752 3158 3402 3403 4504 4970 6777 6995
Size: 862 406 244 1 1101 466 1807 218
Cuts at: 6995 8406 1890
Size: 1411 4751
Fragments arranged by size:
4751 1807 1411 1101 862 466 406 244
218 1
EcoRI G'AATT_C
Cuts at: 1968 6217 8271 1968
Size: 4249 2054 4964
Fragments arranged by size:
4964 4249 2054
EcoRV GAT'ATC
Cuts at: 2351 4641 2351
Size: 2290 8977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 525 2596 3680 6079 6156 9286 525
Size: 2071 1084 2399 77 3130 2506
Fragments arranged by size:
3130 2506 2399 2071 1084 77
FspI TGC'GCA
Cuts at: 9048 9048
Size: 11267
HaeII r_GCGC'y
Cuts at: 560 1607 1637 2726 6023 6865 8701 8949 10179
Size: 1047 30 1089 3297 842 1836 248 1230
Cuts at: 10179 10950 560
Size: 771 877
Fragments arranged by size:
3297 1836 1230 1089 1047 877 842 771
248 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2335 2367 2766 2798 3405 3437 4918 4950 5079
Size: 32 399 32 607 32 1481 32 129
Cuts at: 5079 5111 5608 5640 6909 6941 7887 7919 8136
Size: 32 497 32 1269 32 946 32 217
Cuts at: 8136 8168 9109 9139 9141 9171 2335
Size: 32 941 30 2 30 4431
Fragments arranged by size:
4431 1481 1269 946 941 607 497 399
217 129 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2258 3434 5305 5848 8306 2258
Size: 1176 1871 543 2458 5219
Fragments arranged by size:
5219 2458 1871 1176 543
HindIII A'AGCT_T
Cuts at: 1701 2356 2375 1701
Size: 655 19 10593
Fragments arranged by size:
10593 655 19
KasI G'GCGC_C
Cuts at: 1603 1633 8697 8945 1603
Size: 30 7064 248 3925
Fragments arranged by size:
7064 3925 248 30
KpnI G_GTAC'C
Cuts at: 7330 8287 7330
Size: 957 10310
MfeI C'AATT_G
Cuts at: 4789 4789
Size: 11267
MluI A'CGCG_T
Cuts at: 10700 10700
Size: 11267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1013 1934 4857 5525 7647 10026 10210 1013
Size: 921 2923 668 2122 2379 184 2070
Fragments arranged by size:
2923 2379 2122 2070 921 668 184
MscI TGG'CCA
Cuts at: 2326 9028 2326
Size: 6702 4565
MslI CAynn'nnrTG
Cuts at: 965 2908 5189 5377 6440 6874 7354 7447 9256
Size: 1943 2281 188 1063 434 480 93 1809
Cuts at: 9256 9383 965
Size: 127 2849
Fragments arranged by size:
2849 2281 1943 1809 1063 480 434 188
127 93
MspA1I CmG'CkG
Cuts at: 2415 4153 5614 5898 7120 7144 7929 8190 8378
Size: 1738 1461 284 1222 24 785 261 188
Cuts at: 8378 8692 9052 9836 10081 2415
Size: 314 360 784 245 3601
Fragments arranged by size:
3601 1738 1461 1222 785 784 360 314
284 261 245 188 24
NaeI GCC'GGC
Cuts at: 9448 9448
Size: 11267
NarI GG'CG_CC
Cuts at: 1604 1634 8698 8946 1604
Size: 30 7064 248 3925
Fragments arranged by size:
7064 3925 248 30
NcoI C'CATG_G
Cuts at: 1637 1847 3416 5378 1637
Size: 210 1569 1962 7526
Fragments arranged by size:
7526 1962 1569 210
NdeI CA'TA_TG
Cuts at: 902 902
Size: 11267
NgoMIV G'CCGG_C
Cuts at: 9446 9446
Size: 11267
NheI G'CTAG_C
Cuts at: 6739 6739
Size: 11267
NsiI A_TGCA'T
Cuts at: 646 646
Size: 11267
NspI r_CATG'y
Cuts at: 1352 1932 3763 4363 6414 7844 8320 8820 10423
Size: 580 1831 600 2051 1430 476 500 1603
Cuts at: 10423 1352
Size: 2196
Fragments arranged by size:
2196 2051 1831 1603 1430 600 580 500
476
PasI CC'CwG_GG
Cuts at: 1935 6857 1935
Size: 4922 6345
PciI A'CATG_T
Cuts at: 1928 3759 10419 1928
Size: 1831 6660 2776
Fragments arranged by size:
6660 2776 1831
PflMI CCAn_nnn'nTGG
Cuts at: 1161 1516 5898 1161
Size: 355 4382 6530
Fragments arranged by size:
6530 4382 355
PfoI T'CCnGG_A
Cuts at: 3593 4015 4176 3593
Size: 422 161 10684
Fragments arranged by size:
10684 422 161
PmeI GTTT'AAAC
Cuts at: 8266 8266
Size: 11267
PmlI CAC'GTG
Cuts at: 8037 8037
Size: 11267
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2502 2534 9713 9745 2502
Size: 32 7179 32 4024
Fragments arranged by size:
7179 4024 32 32
PpuMI rG'GwC_Cy
Cuts at: 1890 2752 3158 6777 6995 1890
Size: 862 406 3619 218 6162
Fragments arranged by size:
6162 3619 862 406 218
PsiI TTA'TAA
Cuts at: 1085 7295 1085
Size: 6210 5057
PspOMI G'GGCC_C
Cuts at: 3402 3402
Size: 11267
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7407 7439 7407
Size: 32 11235
PstI C_TGCA'G
Cuts at: 521 1649 1764 2241 3128 3488 4583 5030 7119
Size: 1128 115 477 887 360 1095 447 2089
Cuts at: 7119 7676 7971 8314 8999 521
Size: 557 295 343 685 2789
Fragments arranged by size:
2789 2089 1128 1095 887 685 557 477
447 360 343 295 115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11267
PvuII CAG'CTG
Cuts at: 4153 5614 5898 7120 8692 9052 4153
Size: 1461 284 1222 1572 360 6368
Fragments arranged by size:
6368 1572 1461 1222 360 284
RsrII CG'GwC_CG
Cuts at: 9462 9462
Size: 11267
SacI G_AGCT'C
Cuts at: 4967 7812 8281 4967
Size: 2845 469 7953
Fragments arranged by size:
7953 2845 469
SacII CC_GC'GG
Cuts at: 2416 8191 2416
Size: 5775 5492
SalI G'TCGA_C
Cuts at: 3432 5303 8304 3432
Size: 1871 3001 6395
Fragments arranged by size:
6395 3001 1871
SapI GCTCTTCn'nnn_
Cuts at: 4478 4833 9290 9500 4478
Size: 355 4457 210 6245
Fragments arranged by size:
6245 4457 355 210
SbfI CC_TGCA'GG
Cuts at: 7971 8314 7971
Size: 343 10924
ScaI AGT'ACT
Cuts at: 6789 10784 6789
Size: 3995 7272
SexAI A'CCwGG_T
Cuts at: 2301 7883 2301
Size: 5582 5685
SfcI C'TryA_G
Cuts at: 33 517 1106 1645 1747 1760 2237 3124 3144
Size: 484 589 539 102 13 477 887 20
Cuts at: 3144 3484 4579 5026 5519 6980 7115 7207 7672
Size: 340 1095 447 493 1461 135 92 465
Cuts at: 7672 7868 7919 7967 8310 8995 9963 10154 33
Size: 196 51 48 343 685 968 191 1146
Fragments arranged by size:
1461 1146 1095 968 887 685 589 539
493 484 477 465 447 343 340 196
191 135 102 92 51 48 20 13
SfoI GGC'GCC
Cuts at: 1605 1635 8699 8947 1605
Size: 30 7064 248 3925
Fragments arranged by size:
7064 3925 248 30
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11267
SmaI CCC'GGG
Cuts at: 8289 8289
Size: 11267
SmlI C'TyrA_G
Cuts at: 600 1824 2338 3777 4340 5949 6174 7181 8422
Size: 1224 514 1439 563 1609 225 1007 1241
Cuts at: 8422 9337 10051 10313 600
Size: 915 714 262 1554
Fragments arranged by size:
1609 1554 1439 1241 1224 1007 915 714
563 514 262 225
SpeI A'CTAG_T
Cuts at: 67 4516 5082 67
Size: 4449 566 6252
Fragments arranged by size:
6252 4449 566
SphI G_CATG'C
Cuts at: 7844 8320 8820 7844
Size: 476 500 10291
Fragments arranged by size:
10291 500 476
SspI AAT'ATT
Cuts at: 6305 7599 6305
Size: 1294 9973
StuI AGG'CCT
Cuts at: 1112 3278 7535 7607 1112
Size: 2166 4257 72 4772
Fragments arranged by size:
4772 4257 2166 72
StyI C'CwwG_G
Cuts at: 1637 1847 2877 3416 4974 5378 6518 8401 8755
Size: 210 1030 539 1558 404 1140 1883 354
Cuts at: 8755 1637
Size: 4149
Fragments arranged by size:
4149 1883 1558 1140 1030 539 404 354
210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3957 4463 5905 7912 8850 8983 9442 9651 3957
Size: 506 1442 2007 938 133 459 209 5573
Fragments arranged by size:
5573 2007 1442 938 506 459 209 133
TatI w'GTAC_w
Cuts at: 4443 4519 5515 5770 6787 10782 4443
Size: 76 996 255 1017 3995 4928
Fragments arranged by size:
4928 3995 1017 996 255 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1453 1485 3511 3543 4970 5002 6053 6085 6499
Size: 32 2026 32 1427 32 1051 32 414
Cuts at: 6499 6531 10784 10816 1453
Size: 32 4253 32 1904
Fragments arranged by size:
4253 2026 1904 1427 1051 414 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7899 9064 7899
Size: 1165 10102
XbaI T'CTAG_A
Cuts at: 6760 8298 6760
Size: 1538 9729
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 7620 8762 7620
Size: 1142 10125
XhoI C'TCGA_G
Cuts at: 4340 4340
Size: 11267
XmaI C'CCGG_G
Cuts at: 8287 8287
Size: 11267
XmnI GAAnn'nnTTC
Cuts at: 2517 2625 3233 3278 7040 2517
Size: 108 608 45 3762 6744
Fragments arranged by size:
6744 3762 608 108 45
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AscI AvrII BsiWI BspEI
BssHII BstZ17I EcoNI FseI FspAI HpaI NotI NruI
PacI PshAI PspXI SanDI SfiI SgrAI SnaBI SrfI
SwaI ZraI