Restriction map for
pF1KE0291
(Circular) (Six-base) MAPSORT of: pF1KE0291.seq Check: 2360 from: 1 to: 4334
pF1KE0291 4334 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 597 1372 597
Size: 775 3559
Acc65I G'GTAC_C
Cuts at: 1350 1350
Size: 4334
AclI AA'CG_TT
Cuts at: 4174 4174
Size: 4334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 793 2159 2591 3338 3606 280
Size: 513 1366 432 747 268 1008
Fragments arranged by size:
1366 1008 747 513 432 268
AflIII A'CryG_T
Cuts at: 946 1686 2790 3767 946
Size: 740 1104 977 1513
Fragments arranged by size:
1513 1104 977 740
AgeI A'CCGG_T
Cuts at: 2722 2722
Size: 4334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 521 521
Size: 4334
AlwNI CAG_nnn'CTG
Cuts at: 1217 1639 3206 1217
Size: 422 1567 2345
Fragments arranged by size:
2345 1567 422
ApaLI G'TGCA_C
Cuts at: 3104 3104
Size: 4334
ApoI r'AATT_y
Cuts at: 1338 3687 3943 1338
Size: 2349 256 1729
Fragments arranged by size:
2349 1729 256
AseI AT'TA_AT
Cuts at: 20 842 20
Size: 822 3512
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
AvaI C'yCGr_G
Cuts at: 1354 1354
Size: 4334
BamHI G'GATC_C
Cuts at: 1359 1359
Size: 4334
BanI G'GyrC_C
Cuts at: 925 1350 1764 2012 2047 925
Size: 425 414 248 35 3212
Fragments arranged by size:
3212 425 414 248 35
BanII G_rGCy'C
Cuts at: 268 324 365 452 641 686 1348 2378 268
Size: 56 41 87 189 45 662 1030 2224
Fragments arranged by size:
2224 1030 662 189 87 56 45 41
BbeI G_GCGC'C
Cuts at: 1768 2016 1768
Size: 248 4086
BbsI GAAGACnn'nnnn_
Cuts at: 1087 1087
Size: 4334
BciVI GTATCCnnnnn_n'
Cuts at: 2232 2993 4334 2232
Size: 761 1341 2232
Fragments arranged by size:
2232 1341 761
BclI T'GATC_A
Cuts at: 431 1242 1854 431
Size: 811 612 2911
Fragments arranged by size:
2911 811 612
BglII A'GATC_T
Cuts at: 1849 3886 1849
Size: 2037 2297
BlpI GC'TnA_GC
Cuts at: 343 1446 343
Size: 1103 3231
Bme1580I G_kGCm'C
Cuts at: 1959 2052 3108 1959
Size: 93 1056 3185
Fragments arranged by size:
3185 1056 93
BmgBI CAC'GTC
Cuts at: 947 947
Size: 4334
BmrI ACTGGGnnnn_n'
Cuts at: 446 542 1546 1627 1962 4106 446
Size: 96 1004 81 335 2144 674
Fragments arranged by size:
2144 1004 674 335 96 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 4334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 466 851 172
Size: 294 385 3655
Fragments arranged by size:
3655 385 294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1510 2389 2881 3179 1510
Size: 879 492 298 2665
Fragments arranged by size:
2665 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 649 4038 649
Size: 3389 945
BsaAI yAC'GTr
Cuts at: 1687 1687
Size: 4334
BsaBI GATnn'nnATC
Cuts at: 6 433 1848 3885 6
Size: 427 1415 2037 455
Fragments arranged by size:
2037 1415 455 427
BsaHI Gr'CG_yC
Cuts at: 940 1765 2013 2715 4211 940
Size: 825 248 702 1496 1063
Fragments arranged by size:
1496 1063 825 702 248
BsaWI w'CCGG_w
Cuts at: 678 1523 2044 2722 2996 3143 3554 678
Size: 845 521 678 274 147 411 1458
Fragments arranged by size:
1458 845 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1217 1217
Size: 4334
BseYI C'CCAG_C
Cuts at: 685 1759 2517 3094 3590 685
Size: 1074 758 577 496 1429
Fragments arranged by size:
1429 1074 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 722 270
Size: 452 3882
BsiEI CG_ry'CG
Cuts at: 104 1876 1922 3130 104
Size: 1772 46 1208 1308
Fragments arranged by size:
1772 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 130 641 1348 2126 2316 3108 130
Size: 511 707 778 190 792 1356
Fragments arranged by size:
1356 792 778 707 511 190
BsmBI CGTCTCn'nnnn_
Cuts at: 297 297
Size: 4334
Bsp1286I G_dGCh'C
Cuts at: 130 268 324 365 452 641 686 1348 1959
Size: 138 56 41 87 189 45 662 611
Cuts at: 1959 2052 2126 2316 2378 3108 130
Size: 93 74 190 62 730 1356
Fragments arranged by size:
1356 730 662 611 190 189 138 93
87 74 62 56 45 41
BspEI T'CCGG_A
Cuts at: 678 3554 678
Size: 2876 1458
BspHI T'CATG_A
Cuts at: 3510 3510
Size: 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1384 1598 1900 2281 1384
Size: 214 302 381 3437
Fragments arranged by size:
3437 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2626 2680 4164 4329 50
Size: 2576 54 1484 165 55
Fragments arranged by size:
2576 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 741 886 2246 741
Size: 145 1360 2829
Fragments arranged by size:
2829 1360 145
BsrFI r'CCGG_y
Cuts at: 2332 2513 2722 2332
Size: 181 209 3944
Fragments arranged by size:
3944 209 181
BssSI C'ACGA_G
Cuts at: 2605 2963 2605
Size: 358 3976
BstAPI GCAn_nnn'nTGC
Cuts at: 1004 1711 1004
Size: 707 3627
BstBI TT'CG_AA
Cuts at: 2695 2695
Size: 4334
BstXI CCAn_nnnn'nTGG
Cuts at: 829 829
Size: 4334
BstYI r'GATC_y
Cuts at: 1 147 502 1359 1826 1849 2184 2430 3442
Size: 146 355 857 467 23 335 246 1012
Cuts at: 3442 3528 3540 3886 1
Size: 86 12 346 449
Fragments arranged by size:
1012 857 467 449 355 346 335 246
146 86 23 12
BtgI C'CryG_G
Cuts at: 107 356 107
Size: 249 4085
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 195 2284 2465 2628 195
Size: 2089 181 163 1901
Fragments arranged by size:
2089 1901 181 163
BtsI GCAGTG_nn'
Cuts at: 203 710 1577 203
Size: 507 867 2960
Fragments arranged by size:
2960 867 507
DraI TTT'AAA
Cuts at: 1333 1333
Size: 4334
DraIII CAC_nnn'GTG
Cuts at: 444 444
Size: 4334
DrdI GACnn_nn'nnGTC
Cuts at: 2040 2898 2040
Size: 858 3476
EaeI y'GGCC_r
Cuts at: 1292 1919 2093 2484 2511 3856 1292
Size: 627 174 391 27 1345 1770
Fragments arranged by size:
1770 1345 627 391 174 27
EagI C'GGCC_G
Cuts at: 1919 1919
Size: 4334
EarI CTCTTCn'nnn_
Cuts at: 760 2357 2567 760
Size: 1597 210 2527
Fragments arranged by size:
2527 1597 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2542 2852 2998 4144 2542
Size: 310 146 1146 2732
Fragments arranged by size:
2732 1146 310 146
EcoICRI GAG'CTC
Cuts at: 639 1346 639
Size: 707 3627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 280 466 793 851 2159 2591 3338 3606
Size: 108 186 327 58 1308 432 747 268
Cuts at: 3606 172
Size: 900
Fragments arranged by size:
1308 900 747 432 327 268 186 108
58
EcoNI CCTnn'n_nnAGG
Cuts at: 393 393
Size: 4334
EcoO109I rG'GnC_Cy
Cuts at: 238 1473 238
Size: 1235 3099
EcoRI G'AATT_C
Cuts at: 1338 1338
Size: 4334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 928 2353 928
Size: 1425 2909
FspI TGC'GCA
Cuts at: 2115 2115
Size: 4334
HaeII r_GCGC'y
Cuts at: 1768 2016 3038 4017 1768
Size: 248 1022 979 2085
Fragments arranged by size:
2085 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 464 496 801 833 936 968 2176 2206 2208
Size: 32 305 32 103 32 1208 30 2
Cuts at: 2208 2238 464
Size: 30 2560
Fragments arranged by size:
2560 1208 305 103 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1373 1373
Size: 4334
KasI G'GCGC_C
Cuts at: 1764 2012 1764
Size: 248 4086
KpnI G_GTAC'C
Cuts at: 1354 1354
Size: 4334
MluI A'CGCG_T
Cuts at: 3767 3767
Size: 4334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 888 3005 3189 888
Size: 2117 184 2033
Fragments arranged by size:
2117 2033 184
MscI TGG'CCA
Cuts at: 1294 2095 1294
Size: 801 3533
MslI CAynn'nnrTG
Cuts at: 337 2323 2450 337
Size: 1986 127 2221
Fragments arranged by size:
2221 1986 127
MspA1I CmG'CkG
Cuts at: 169 1236 1445 1759 2119 3132 3377 169
Size: 1067 209 314 360 1013 245 1126
Fragments arranged by size:
1126 1067 1013 360 314 245 209
NaeI GCC'GGC
Cuts at: 2515 2515
Size: 4334
NarI GG'CG_CC
Cuts at: 1765 2013 1765
Size: 248 4086
NcoI C'CATG_G
Cuts at: 107 356 107
Size: 249 4085
NgoMIV G'CCGG_C
Cuts at: 2513 2513
Size: 4334
NspI r_CATG'y
Cuts at: 1387 1887 2794 1387
Size: 500 907 2927
Fragments arranged by size:
2927 907 500
PasI CC'CwG_GG
Cuts at: 297 297
Size: 4334
PciI A'CATG_T
Cuts at: 2790 2790
Size: 4334
PflMI CCAn_nnn'nTGG
Cuts at: 444 528 444
Size: 84 4250
PfoI T'CCnGG_A
Cuts at: 620 620
Size: 4334
PmeI GTTT'AAAC
Cuts at: 1333 1333
Size: 4334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 406 438 3473 3505 406
Size: 32 3035 32 1235
Fragments arranged by size:
3035 1235 32 32
PstI C_TGCA'G
Cuts at: 1381 2066 1381
Size: 685 3649
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4334
PvuII CAG'CTG
Cuts at: 1759 2119 1759
Size: 360 3974
RsrII CG'GwC_CG
Cuts at: 2529 2529
Size: 4334
SacI G_AGCT'C
Cuts at: 641 1348 641
Size: 707 3627
SalI G'TCGA_C
Cuts at: 1371 1371
Size: 4334
SapI GCTCTTCn'nnn_
Cuts at: 760 2357 2567 760
Size: 1597 210 2527
Fragments arranged by size:
2527 1597 210
SbfI CC_TGCA'GG
Cuts at: 1381 1381
Size: 4334
ScaI AGT'ACT
Cuts at: 3851 3851
Size: 4334
SfcI C'TryA_G
Cuts at: 33 1026 1322 1377 2062 3055 3246 33
Size: 993 296 55 685 993 191 1121
Fragments arranged by size:
1121 993 993 685 296 191 55
SfoI GGC'GCC
Cuts at: 1766 2014 1766
Size: 248 4086
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
SmaI CCC'GGG
Cuts at: 1356 1356
Size: 4334
SmlI C'TyrA_G
Cuts at: 1489 2404 2896 3158 1489
Size: 915 492 262 2665
Fragments arranged by size:
2665 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4334
SphI G_CATG'C
Cuts at: 1387 1887 1387
Size: 500 3834
SspI AAT'ATT
Cuts at: 846 846
Size: 4334
StyI C'CwwG_G
Cuts at: 107 356 1468 1822 107
Size: 249 1112 354 2619
Fragments arranged by size:
2619 1112 354 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 631 1269 1917 2050 2509 2718 631
Size: 638 648 133 459 209 2247
Fragments arranged by size:
2247 648 638 459 209 133
TatI w'GTAC_w
Cuts at: 949 3849 949
Size: 2900 1434
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4302
Tth111I GACn'n_nGTC
Cuts at: 2131 2131
Size: 4334
XbaI T'CTAG_A
Cuts at: 1365 1365
Size: 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1829 1829
Size: 4334
XmaI C'CCGG_G
Cuts at: 1354 1354
Size: 4334
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI