Restriction map for
pF1KE0286
(Circular) (Six-base) MAPSORT of: pF1KE0286.seq Check: 8654 from: 1 to: 3320
pF1KE0286 3320 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 358 358
Size: 3320
Acc65I G'GTAC_C
Cuts at: 336 336
Size: 3320
AclI AA'CG_TT
Cuts at: 201 3160 201
Size: 2959 361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 1577 2324 2592 1145
Size: 432 747 268 1873
Fragments arranged by size:
1873 747 432 268
AflIII A'CryG_T
Cuts at: 166 672 1776 2753 166
Size: 506 1104 977 733
Fragments arranged by size:
1104 977 733 506
AgeI A'CCGG_T
Cuts at: 1708 1708
Size: 3320
AlwNI CAG_nnn'CTG
Cuts at: 625 2192 625
Size: 1567 1753
ApaLI G'TGCA_C
Cuts at: 2090 2090
Size: 3320
ApoI r'AATT_y
Cuts at: 272 324 2673 2929 272
Size: 52 2349 256 663
Fragments arranged by size:
2349 663 256 52
AseI AT'TA_AT
Cuts at: 20 20
Size: 3320
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3320
AvaI C'yCGr_G
Cuts at: 340 340
Size: 3320
BamHI G'GATC_C
Cuts at: 345 345
Size: 3320
BanI G'GyrC_C
Cuts at: 336 750 998 1033 336
Size: 414 248 35 2623
Fragments arranged by size:
2623 414 248 35
BanII G_rGCy'C
Cuts at: 334 1364 334
Size: 1030 2290
BbeI G_GCGC'C
Cuts at: 754 1002 754
Size: 248 3072
BciVI GTATCCnnnnn_n'
Cuts at: 1218 1979 3320 1218
Size: 761 1341 1218
Fragments arranged by size:
1341 1218 761
BclI T'GATC_A
Cuts at: 840 840
Size: 3320
BglII A'GATC_T
Cuts at: 835 2872 835
Size: 2037 1283
BlpI GC'TnA_GC
Cuts at: 432 432
Size: 3320
Bme1580I G_kGCm'C
Cuts at: 945 1038 2094 945
Size: 93 1056 2171
Fragments arranged by size:
2171 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 532 613 948 3092 532
Size: 81 335 2144 760
Fragments arranged by size:
2144 760 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1687 1687
Size: 3320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 496 1375 1867 2165 250
Size: 246 879 492 298 1405
Fragments arranged by size:
1405 879 492 298 246
BsaI GGTCTCn'nnnn_
Cuts at: 3024 3024
Size: 3320
BsaAI yAC'GTr
Cuts at: 673 673
Size: 3320
BsaBI GATnn'nnATC
Cuts at: 6 834 2871 6
Size: 828 2037 455
Fragments arranged by size:
2037 828 455
BsaHI Gr'CG_yC
Cuts at: 751 999 1701 3197 751
Size: 248 702 1496 874
Fragments arranged by size:
1496 874 702 248
BsaWI w'CCGG_w
Cuts at: 509 1030 1708 1982 2129 2540 509
Size: 521 678 274 147 411 1289
Fragments arranged by size:
1289 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 745 1503 2080 2576 745
Size: 758 577 496 1489
Fragments arranged by size:
1489 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 264
Size: 3320
BsiEI CG_ry'CG
Cuts at: 104 210 862 908 2116 104
Size: 106 652 46 1208 1308
Fragments arranged by size:
1308 1208 652 106 46
BsiHKAI G_wGCw'C
Cuts at: 334 1112 1302 2094 334
Size: 778 190 792 1560
Fragments arranged by size:
1560 792 778 190
BsmI GAATG_Cn'
Cuts at: 186 186
Size: 3320
Bsp1286I G_dGCh'C
Cuts at: 334 945 1038 1112 1302 1364 2094 334
Size: 611 93 74 190 62 730 1560
Fragments arranged by size:
1560 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2540 2540
Size: 3320
BspHI T'CATG_A
Cuts at: 2496 2496
Size: 3320
BspMI ACCTGCnnnn'nnnn_
Cuts at: 370 584 886 1267 370
Size: 214 302 381 2423
Fragments arranged by size:
2423 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1612 1666 3150 3315 50
Size: 1562 54 1484 165 55
Fragments arranged by size:
1562 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1232 1232
Size: 3320
BsrFI r'CCGG_y
Cuts at: 1318 1499 1708 1318
Size: 181 209 2930
Fragments arranged by size:
2930 209 181
BssSI C'ACGA_G
Cuts at: 1591 1949 1591
Size: 358 2962
BstAPI GCAn_nnn'nTGC
Cuts at: 697 697
Size: 3320
BstBI TT'CG_AA
Cuts at: 1681 1681
Size: 3320
BstYI r'GATC_y
Cuts at: 1 113 345 812 835 1170 1416 2428 2514
Size: 112 232 467 23 335 246 1012 86
Cuts at: 2514 2526 2872 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 246 232 112
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1270 1451 1614 1270
Size: 181 163 2976
Fragments arranged by size:
2976 181 163
BtsI GCAGTG_nn'
Cuts at: 563 563
Size: 3320
DraI TTT'AAA
Cuts at: 319 319
Size: 3320
DrdI GACnn_nn'nnGTC
Cuts at: 1026 1884 1026
Size: 858 2462
EaeI y'GGCC_r
Cuts at: 905 1079 1470 1497 2842 905
Size: 174 391 27 1345 1383
Fragments arranged by size:
1383 1345 391 174 27
EagI C'GGCC_G
Cuts at: 905 905
Size: 3320
EarI CTCTTCn'nnn_
Cuts at: 1343 1553 1343
Size: 210 3110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1528 1838 1984 3130 1528
Size: 310 146 1146 1718
Fragments arranged by size:
1718 1146 310 146
EcoICRI GAG'CTC
Cuts at: 332 332
Size: 3320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 1577 2324 2592 1145
Size: 432 747 268 1873
Fragments arranged by size:
1873 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 459 459
Size: 3320
EcoRI G'AATT_C
Cuts at: 324 324
Size: 3320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1339 1339
Size: 3320
FspI TGC'GCA
Cuts at: 1101 1101
Size: 3320
HaeII r_GCGC'y
Cuts at: 754 1002 2024 3003 754
Size: 248 1022 979 1071
Fragments arranged by size:
1071 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1162 1192 1194 1224 1162
Size: 30 2 30 3258
Fragments arranged by size:
3258 30 30 2
HincII GTy'rAC
Cuts at: 359 359
Size: 3320
KasI G'GCGC_C
Cuts at: 750 998 750
Size: 248 3072
KpnI G_GTAC'C
Cuts at: 340 340
Size: 3320
MluI A'CGCG_T
Cuts at: 2753 2753
Size: 3320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1991 2175 1991
Size: 184 3136
MscI TGG'CCA
Cuts at: 1081 1081
Size: 3320
MslI CAynn'nnrTG
Cuts at: 1309 1436 1309
Size: 127 3193
MspA1I CmG'CkG
Cuts at: 431 745 1105 2118 2363 431
Size: 314 360 1013 245 1388
Fragments arranged by size:
1388 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1501 1501
Size: 3320
NarI GG'CG_CC
Cuts at: 751 999 751
Size: 248 3072
NdeI CA'TA_TG
Cuts at: 253 253
Size: 3320
NgoMIV G'CCGG_C
Cuts at: 1499 1499
Size: 3320
NspI r_CATG'y
Cuts at: 170 373 873 1780 170
Size: 203 500 907 1710
Fragments arranged by size:
1710 907 500 203
PciI A'CATG_T
Cuts at: 166 1776 166
Size: 1610 1710
PmeI GTTT'AAAC
Cuts at: 319 319
Size: 3320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2459 2491 2459
Size: 32 3288
PstI C_TGCA'G
Cuts at: 367 1052 367
Size: 685 2635
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3320
PvuII CAG'CTG
Cuts at: 745 1105 745
Size: 360 2960
RsrII CG'GwC_CG
Cuts at: 1515 1515
Size: 3320
SacI G_AGCT'C
Cuts at: 334 334
Size: 3320
SalI G'TCGA_C
Cuts at: 357 357
Size: 3320
SapI GCTCTTCn'nnn_
Cuts at: 1343 1553 1343
Size: 210 3110
SbfI CC_TGCA'GG
Cuts at: 367 367
Size: 3320
ScaI AGT'ACT
Cuts at: 2837 2837
Size: 3320
SfcI C'TryA_G
Cuts at: 33 363 1048 2041 2232 33
Size: 330 685 993 191 1121
Fragments arranged by size:
1121 993 685 330 191
SfoI GGC'GCC
Cuts at: 752 1000 752
Size: 248 3072
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3320
SmaI CCC'GGG
Cuts at: 342 342
Size: 3320
SmlI C'TyrA_G
Cuts at: 229 475 1390 1882 2144 229
Size: 246 915 492 262 1405
Fragments arranged by size:
1405 915 492 262 246
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3320
SphI G_CATG'C
Cuts at: 373 873 373
Size: 500 2820
StyI C'CwwG_G
Cuts at: 173 454 808 173
Size: 281 354 2685
Fragments arranged by size:
2685 354 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 903 1036 1495 1704 903
Size: 133 459 209 2519
Fragments arranged by size:
2519 459 209 133
TatI w'GTAC_w
Cuts at: 2835 2835
Size: 3320
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 3288
Tth111I GACn'n_nGTC
Cuts at: 1117 1117
Size: 3320
XbaI T'CTAG_A
Cuts at: 351 351
Size: 3320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 171 815 171
Size: 644 2676
XmaI C'CCGG_G
Cuts at: 340 340
Size: 3320
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI