Restriction map for
pF1KE0285
(Circular) (Six-base) MAPSORT of: pF1KE0285.seq Check: 8576 from: 1 to: 3776
pF1KE0285 3776 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AatII G_ACGT'C
Cuts at: 350 350
Size: 3776
AccI GT'mk_AC
Cuts at: 264 814 264
Size: 550 3226
Acc65I G'GTAC_C
Cuts at: 792 792
Size: 3776
AclI AA'CG_TT
Cuts at: 3616 3616
Size: 3776
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 1601 2033 2780 3048 205
Size: 1396 432 747 268 933
Fragments arranged by size:
1396 933 747 432 268
AflIII A'CryG_T
Cuts at: 1128 2232 3209 1128
Size: 1104 977 1695
Fragments arranged by size:
1695 1104 977
AgeI A'CCGG_T
Cuts at: 2164 2164
Size: 3776
AleI CACnn'nnGTG
Cuts at: 172 172
Size: 3776
AlwNI CAG_nnn'CTG
Cuts at: 1081 2648 1081
Size: 1567 2209
ApaI G_GGCC'C
Cuts at: 764 764
Size: 3776
ApaLI G'TGCA_C
Cuts at: 2546 2546
Size: 3776
ApoI r'AATT_y
Cuts at: 780 3129 3385 780
Size: 2349 256 1171
Fragments arranged by size:
2349 1171 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3776
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
AvaI C'yCGr_G
Cuts at: 796 796
Size: 3776
BamHI G'GATC_C
Cuts at: 220 801 220
Size: 581 3195
BanI G'GyrC_C
Cuts at: 792 1206 1454 1489 792
Size: 414 248 35 3079
Fragments arranged by size:
3079 414 248 35
BanII G_rGCy'C
Cuts at: 764 790 1820 764
Size: 26 1030 2720
Fragments arranged by size:
2720 1030 26
BbeI G_GCGC'C
Cuts at: 1210 1458 1210
Size: 248 3528
BbsI GAAGACnn'nnnn_
Cuts at: 193 460 193
Size: 267 3509
BbvCI CC'TCA_GC
Cuts at: 277 277
Size: 3776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3742
BciVI GTATCCnnnnn_n'
Cuts at: 615 1674 2435 3776 615
Size: 1059 761 1341 615
Fragments arranged by size:
1341 1059 761 615
BclI T'GATC_A
Cuts at: 1296 1296
Size: 3776
BglII A'GATC_T
Cuts at: 1291 3328 1291
Size: 2037 1739
BlpI GC'TnA_GC
Cuts at: 888 888
Size: 3776
Bme1580I G_kGCm'C
Cuts at: 764 1401 1494 2550 764
Size: 637 93 1056 1990
Fragments arranged by size:
1990 1056 637 93
BmrI ACTGGGnnnn_n'
Cuts at: 573 748 988 1069 1404 3548 573
Size: 175 240 81 335 2144 801
Fragments arranged by size:
2144 801 335 240 175 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 3776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 267
Size: 3776
Bpu10I CC'TnA_GC
Cuts at: 277 277
Size: 3776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 952 1831 2323 2621 635
Size: 317 879 492 298 1790
Fragments arranged by size:
1790 879 492 317 298
BsaI GGTCTCn'nnnn_
Cuts at: 206 3480 206
Size: 3274 502
BsaAI yAC'GTr
Cuts at: 1129 1129
Size: 3776
BsaBI GATnn'nnATC
Cuts at: 6 1290 3327 6
Size: 1284 2037 455
Fragments arranged by size:
2037 1284 455
BsaHI Gr'CG_yC
Cuts at: 347 1207 1455 2157 3653 347
Size: 860 248 702 1496 470
Fragments arranged by size:
1496 860 702 470 248
BsaWI w'CCGG_w
Cuts at: 965 1486 2164 2438 2585 2996 965
Size: 521 678 274 147 411 1745
Fragments arranged by size:
1745 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 118 118
Size: 3776
BseYI C'CCAG_C
Cuts at: 144 1201 1959 2536 3032 144
Size: 1057 758 577 496 888
Fragments arranged by size:
1057 888 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1318 1364 2572 104
Size: 1214 46 1208 1308
Fragments arranged by size:
1308 1214 1208 46
BsiHKAI G_wGCw'C
Cuts at: 651 790 1568 1758 2550 651
Size: 139 778 190 792 1877
Fragments arranged by size:
1877 792 778 190 139
Bsp1286I G_dGCh'C
Cuts at: 651 764 790 1401 1494 1568 1758 1820 2550
Size: 113 26 611 93 74 190 62 730
Cuts at: 2550 651
Size: 1877
Fragments arranged by size:
1877 730 611 190 113 93 74 62
26
BspEI T'CCGG_A
Cuts at: 2996 2996
Size: 3776
BspHI T'CATG_A
Cuts at: 429 2952 429
Size: 2523 1253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 826 1040 1342 1723 826
Size: 214 302 381 2879
Fragments arranged by size:
2879 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2068 2122 3606 3771 50
Size: 2018 54 1484 165 55
Fragments arranged by size:
2018 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1688 1688
Size: 3776
BsrFI r'CCGG_y
Cuts at: 1774 1955 2164 1774
Size: 181 209 3386
Fragments arranged by size:
3386 209 181
BsrGI T'GTAC_A
Cuts at: 231 231
Size: 3776
BssSI C'ACGA_G
Cuts at: 2047 2405 2047
Size: 358 3418
BstAPI GCAn_nnn'nTGC
Cuts at: 495 1153 495
Size: 658 3118
BstBI TT'CG_AA
Cuts at: 2137 2137
Size: 3776
BstEII G'GTnAC_C
Cuts at: 718 718
Size: 3776
BstYI r'GATC_y
Cuts at: 1 220 801 1268 1291 1626 1872 2884 2970
Size: 219 581 467 23 335 246 1012 86
Cuts at: 2970 2982 3328 1
Size: 12 346 449
Fragments arranged by size:
1012 581 467 449 346 335 246 219
86 23 12
BtgI C'CryG_G
Cuts at: 224 224
Size: 3776
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1726 1907 2070 1726
Size: 181 163 3432
Fragments arranged by size:
3432 181 163
BtsI GCAGTG_nn'
Cuts at: 1019 1019
Size: 3776
DraI TTT'AAA
Cuts at: 775 775
Size: 3776
DraIII CAC_nnn'GTG
Cuts at: 756 756
Size: 3776
DrdI GACnn_nn'nnGTC
Cuts at: 1482 2340 1482
Size: 858 2918
EaeI y'GGCC_r
Cuts at: 1361 1535 1926 1953 3298 1361
Size: 174 391 27 1345 1839
Fragments arranged by size:
1839 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1361 1361
Size: 3776
EarI CTCTTCn'nnn_
Cuts at: 1799 2009 1799
Size: 210 3566
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1984 2294 2440 3586 1984
Size: 310 146 1146 2174
Fragments arranged by size:
2174 1146 310 146
EcoICRI GAG'CTC
Cuts at: 788 788
Size: 3776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 267 1601 2033 2780 3048 205
Size: 62 1334 432 747 268 933
Fragments arranged by size:
1334 933 747 432 268 62
EcoO109I rG'GnC_Cy
Cuts at: 760 761 915 760
Size: 1 154 3621
Fragments arranged by size:
3621 154 1
EcoRI G'AATT_C
Cuts at: 780 780
Size: 3776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 210 329 1795 210
Size: 119 1466 2191
Fragments arranged by size:
2191 1466 119
FspI TGC'GCA
Cuts at: 488 1557 488
Size: 1069 2707
HaeII r_GCGC'y
Cuts at: 1210 1458 2480 3459 1210
Size: 248 1022 979 1527
Fragments arranged by size:
1527 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 377 409 1618 1648 1650 1680 377
Size: 32 1209 30 2 30 2473
Fragments arranged by size:
2473 1209 32 30 30 2
HincII GTy'rAC
Cuts at: 815 815
Size: 3776
KasI G'GCGC_C
Cuts at: 1206 1454 1206
Size: 248 3528
KpnI G_GTAC'C
Cuts at: 796 796
Size: 3776
MluI A'CGCG_T
Cuts at: 3209 3209
Size: 3776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 278 2447 2631 278
Size: 2169 184 1423
Fragments arranged by size:
2169 1423 184
MscI TGG'CCA
Cuts at: 1537 1537
Size: 3776
MslI CAynn'nnrTG
Cuts at: 172 289 1765 1892 172
Size: 117 1476 127 2056
Fragments arranged by size:
2056 1476 127 117
MspA1I CmG'CkG
Cuts at: 887 1201 1561 2574 2819 887
Size: 314 360 1013 245 1844
Fragments arranged by size:
1844 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1957 1957
Size: 3776
NarI GG'CG_CC
Cuts at: 1207 1455 1207
Size: 248 3528
NcoI C'CATG_G
Cuts at: 224 224
Size: 3776
NgoMIV G'CCGG_C
Cuts at: 1955 1955
Size: 3776
NspI r_CATG'y
Cuts at: 370 829 1329 2236 370
Size: 459 500 907 1910
Fragments arranged by size:
1910 907 500 459
PciI A'CATG_T
Cuts at: 2232 2232
Size: 3776
PflMI CCAn_nnn'nTGG
Cuts at: 756 756
Size: 3776
PmeI GTTT'AAAC
Cuts at: 775 775
Size: 3776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2915 2947 2915
Size: 32 3744
PspOMI G'GGCC_C
Cuts at: 760 760
Size: 3776
PstI C_TGCA'G
Cuts at: 662 823 1508 662
Size: 161 685 2930
Fragments arranged by size:
2930 685 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3776
PvuII CAG'CTG
Cuts at: 1201 1561 1201
Size: 360 3416
RsrII CG'GwC_CG
Cuts at: 1971 1971
Size: 3776
SacI G_AGCT'C
Cuts at: 790 790
Size: 3776
SalI G'TCGA_C
Cuts at: 813 813
Size: 3776
SapI GCTCTTCn'nnn_
Cuts at: 1799 2009 1799
Size: 210 3566
SbfI CC_TGCA'GG
Cuts at: 823 823
Size: 3776
ScaI AGT'ACT
Cuts at: 3293 3293
Size: 3776
SfcI C'TryA_G
Cuts at: 33 637 658 690 819 1504 2497 2688 33
Size: 604 21 32 129 685 993 191 1121
Fragments arranged by size:
1121 993 685 604 191 129 32 21
SfoI GGC'GCC
Cuts at: 1208 1456 1208
Size: 248 3528
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
SmaI CCC'GGG
Cuts at: 798 798
Size: 3776
SmlI C'TyrA_G
Cuts at: 650 931 1846 2338 2600 650
Size: 281 915 492 262 1826
Fragments arranged by size:
1826 915 492 281 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3776
SphI G_CATG'C
Cuts at: 829 1329 829
Size: 500 3276
StyI C'CwwG_G
Cuts at: 224 910 1264 224
Size: 686 354 2736
Fragments arranged by size:
2736 686 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 767 1359 1492 1951 2160 767
Size: 592 133 459 209 2383
Fragments arranged by size:
2383 592 459 209 133
TatI w'GTAC_w
Cuts at: 231 3291 231
Size: 3060 716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 3744
Tth111I GACn'n_nGTC
Cuts at: 1573 1573
Size: 3776
XbaI T'CTAG_A
Cuts at: 807 807
Size: 3776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1271 1271
Size: 3776
XmaI C'CCGG_G
Cuts at: 796 796
Size: 3776
ZraI GAC'GTC
Cuts at: 348 348
Size: 3776
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI