Restriction map for
pF1KE0278
(Circular) (Six-base) MAPSORT of: pF1KE0278.seq Check: 9729 from: 1 to: 3746
pF1KE0278 3746 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 712 712
Size: 3746
AccI GT'mk_AC
Cuts at: 644 784 644
Size: 140 3606
Acc65I G'GTAC_C
Cuts at: 762 762
Size: 3746
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 1571 2003 2750 3018 331
Size: 1240 432 747 268 1059
Fragments arranged by size:
1240 1059 747 432 268
AflIII A'CryG_T
Cuts at: 1098 2202 3179 1098
Size: 1104 977 1665
Fragments arranged by size:
1665 1104 977
AgeI A'CCGG_T
Cuts at: 2134 2134
Size: 3746
AlwNI CAG_nnn'CTG
Cuts at: 1051 2618 1051
Size: 1567 2179
ApaI G_GGCC'C
Cuts at: 187 658 187
Size: 471 3275
ApaLI G'TGCA_C
Cuts at: 2516 2516
Size: 3746
ApoI r'AATT_y
Cuts at: 750 3099 3355 750
Size: 2349 256 1141
Fragments arranged by size:
2349 1141 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 766 766
Size: 3746
BamHI G'GATC_C
Cuts at: 771 771
Size: 3746
BanI G'GyrC_C
Cuts at: 762 1176 1424 1459 762
Size: 414 248 35 3049
Fragments arranged by size:
3049 414 248 35
BanII G_rGCy'C
Cuts at: 187 658 760 1790 187
Size: 471 102 1030 2143
Fragments arranged by size:
2143 1030 471 102
BbeI G_GCGC'C
Cuts at: 1180 1428 1180
Size: 248 3498
BciVI GTATCCnnnnn_n'
Cuts at: 1644 2405 3746 1644
Size: 761 1341 1644
Fragments arranged by size:
1644 1341 761
BclI T'GATC_A
Cuts at: 495 1266 495
Size: 771 2975
BfrBI ATG'CAT
Cuts at: 569 569
Size: 3746
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 858 858
Size: 3746
Bme1580I G_kGCm'C
Cuts at: 187 658 1371 1464 2520 187
Size: 471 713 93 1056 1413
Fragments arranged by size:
1413 1056 713 471 93
BmrI ACTGGGnnnn_n'
Cuts at: 958 1039 1374 3518 958
Size: 81 335 2144 1186
Fragments arranged by size:
2144 1186 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 3746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 204
Size: 3746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2293 2591 922
Size: 879 492 298 2077
Fragments arranged by size:
2077 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 3746
BsaAI yAC'GTr
Cuts at: 1099 1099
Size: 3746
BsaBI GATnn'nnATC
Cuts at: 6 1260 3297 6
Size: 1254 2037 455
Fragments arranged by size:
2037 1254 455
BsaHI Gr'CG_yC
Cuts at: 1177 1425 2127 3623 1177
Size: 248 702 1496 1300
Fragments arranged by size:
1496 1300 702 248
BsaWI w'CCGG_w
Cuts at: 935 1456 2134 2408 2555 2966 935
Size: 521 678 274 147 411 1715
Fragments arranged by size:
1715 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 354 634 709 354
Size: 280 75 3391
Fragments arranged by size:
3391 280 75
BseYI C'CCAG_C
Cuts at: 384 720 1171 1929 2506 3002 384
Size: 336 451 758 577 496 1128
Fragments arranged by size:
1128 758 577 496 451 336
BsiEI CG_ry'CG
Cuts at: 104 1288 1334 2542 104
Size: 1184 46 1208 1308
Fragments arranged by size:
1308 1208 1184 46
BsiHKAI G_wGCw'C
Cuts at: 760 1538 1728 2520 760
Size: 778 190 792 1986
Fragments arranged by size:
1986 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 187 658 760 1371 1464 1538 1728 1790 2520
Size: 471 102 611 93 74 190 62 730
Cuts at: 2520 187
Size: 1413
Fragments arranged by size:
1413 730 611 471 190 102 93 74
62
BspEI T'CCGG_A
Cuts at: 2966 2966
Size: 3746
BspHI T'CATG_A
Cuts at: 599 2922 599
Size: 2323 1423
BspMI ACCTGCnnnn'nnnn_
Cuts at: 712 796 1010 1312 1693 712
Size: 84 214 302 381 2765
Fragments arranged by size:
2765 381 302 214 84
BsrBI CCG'CTC
Cuts at: 50 2038 2092 3576 3741 50
Size: 1988 54 1484 165 55
Fragments arranged by size:
1988 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1658 1658
Size: 3746
BsrFI r'CCGG_y
Cuts at: 1744 1925 2134 1744
Size: 181 209 3356
Fragments arranged by size:
3356 209 181
BssSI C'ACGA_G
Cuts at: 2017 2375 2017
Size: 358 3388
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 1123
Size: 3746
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstEII G'GTnAC_C
Cuts at: 699 699
Size: 3746
BstYI r'GATC_y
Cuts at: 1 771 1238 1261 1596 1842 2854 2940 2952
Size: 770 467 23 335 246 1012 86 12
Cuts at: 2952 3298 1
Size: 346 449
Fragments arranged by size:
1012 770 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 692 692
Size: 3746
BtgI C'CryG_G
Cuts at: 107 254 317 393 627 107
Size: 147 63 76 234 3226
Fragments arranged by size:
3226 234 147 76 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1696 1877 2040 1696
Size: 181 163 3402
Fragments arranged by size:
3402 181 163
BtsI GCAGTG_nn'
Cuts at: 537 989 537
Size: 452 3294
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 1452 2310 1452
Size: 858 2888
EaeI y'GGCC_r
Cuts at: 257 396 1331 1505 1896 1923 3268 257
Size: 139 935 174 391 27 1345 735
Fragments arranged by size:
1345 935 735 391 174 139 27
EagI C'GGCC_G
Cuts at: 1331 1331
Size: 3746
EarI CTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1954 2264 2410 3556 1954
Size: 310 146 1146 2144
Fragments arranged by size:
2144 1146 310 146
EcoICRI GAG'CTC
Cuts at: 758 758
Size: 3746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 331 1571 2003 2750 3018 204
Size: 127 1240 432 747 268 932
Fragments arranged by size:
1240 932 747 432 268 127
EcoO109I rG'GnC_Cy
Cuts at: 183 197 654 655 885 183
Size: 14 457 1 230 3044
Fragments arranged by size:
3044 457 230 14 1
EcoRI G'AATT_C
Cuts at: 750 750
Size: 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 264 1765 264
Size: 1501 2245
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 347 727 1180 1428 2450 3429 347
Size: 380 453 248 1022 979 664
Fragments arranged by size:
1022 979 664 453 380 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1588 1618 1620 1650 1588
Size: 30 2 30 3684
Fragments arranged by size:
3684 30 30 2
HincII GTy'rAC
Cuts at: 785 785
Size: 3746
KasI G'GCGC_C
Cuts at: 1176 1424 1176
Size: 248 3498
KpnI G_GTAC'C
Cuts at: 766 766
Size: 3746
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2417 2601 2417
Size: 184 3562
MscI TGG'CCA
Cuts at: 259 398 1507 259
Size: 139 1109 2498
Fragments arranged by size:
2498 1109 139
MslI CAynn'nnrTG
Cuts at: 479 570 1735 1862 479
Size: 91 1165 127 2363
Fragments arranged by size:
2363 1165 127 91
MspA1I CmG'CkG
Cuts at: 857 1171 1531 2544 2789 857
Size: 314 360 1013 245 1814
Fragments arranged by size:
1814 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1927 1927
Size: 3746
NarI GG'CG_CC
Cuts at: 1177 1425 1177
Size: 248 3498
NcoI C'CATG_G
Cuts at: 107 317 107
Size: 210 3536
NgoMIV G'CCGG_C
Cuts at: 1925 1925
Size: 3746
NsiI A_TGCA'T
Cuts at: 571 571
Size: 3746
NspI r_CATG'y
Cuts at: 569 573 799 1299 2206 569
Size: 4 226 500 907 2109
Fragments arranged by size:
2109 907 500 226 4
PciI A'CATG_T
Cuts at: 2202 2202
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 3714
PspOMI G'GGCC_C
Cuts at: 183 654 183
Size: 471 3275
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 595 627 595
Size: 32 3714
PstI C_TGCA'G
Cuts at: 532 673 709 793 1478 532
Size: 141 36 84 685 2800
Fragments arranged by size:
2800 685 141 84 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 1171 1531 1171
Size: 360 3386
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 760 760
Size: 3746
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SfcI C'TryA_G
Cuts at: 33 528 669 705 789 1474 2467 2658 33
Size: 495 141 36 84 685 993 191 1121
Fragments arranged by size:
1121 993 685 495 191 141 84 36
SfoI GGC'GCC
Cuts at: 1178 1426 1178
Size: 248 3498
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 768 768
Size: 3746
SmlI C'TyrA_G
Cuts at: 901 1816 2308 2570 901
Size: 915 492 262 2077
Fragments arranged by size:
2077 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 799 1299 799
Size: 500 3246
StyI C'CwwG_G
Cuts at: 107 317 404 465 880 1234 107
Size: 210 87 61 415 354 2619
Fragments arranged by size:
2619 415 354 210 87 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 164 1329 1462 1921 2130 164
Size: 1165 133 459 209 1780
Fragments arranged by size:
1780 1165 459 209 133
TatI w'GTAC_w
Cuts at: 3261 3261
Size: 3746
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 1543 1543
Size: 3746
XbaI T'CTAG_A
Cuts at: 777 777
Size: 3746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 228 411 1241 228
Size: 183 830 2733
Fragments arranged by size:
2733 830 183
XmaI C'CCGG_G
Cuts at: 766 766
Size: 3746
XmnI GAAnn'nnTTC
Cuts at: 244 247 244
Size: 3 3743
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PmeI PpiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI