Restriction map for
pF1KE0273
(Circular) (Six-base) MAPSORT of: pF1KE0273.seq Check: 5463 from: 1 to: 3422
pF1KE0273 3422 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 460 460
Size: 3422
Acc65I G'GTAC_C
Cuts at: 438 438
Size: 3422
AclI AA'CG_TT
Cuts at: 3262 3262
Size: 3422
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1247 1679 2426 2694 1247
Size: 432 747 268 1975
Fragments arranged by size:
1975 747 432 268
AflIII A'CryG_T
Cuts at: 774 1878 2855 774
Size: 1104 977 1341
Fragments arranged by size:
1341 1104 977
AgeI A'CCGG_T
Cuts at: 1810 1810
Size: 3422
AlwNI CAG_nnn'CTG
Cuts at: 727 2294 727
Size: 1567 1855
ApaLI G'TGCA_C
Cuts at: 2192 2192
Size: 3422
ApoI r'AATT_y
Cuts at: 426 2775 3031 426
Size: 2349 256 817
Fragments arranged by size:
2349 817 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3422
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3422
AvaI C'yCGr_G
Cuts at: 442 442
Size: 3422
BamHI G'GATC_C
Cuts at: 447 447
Size: 3422
BanI G'GyrC_C
Cuts at: 151 438 852 1100 1135 151
Size: 287 414 248 35 2438
Fragments arranged by size:
2438 414 287 248 35
BanII G_rGCy'C
Cuts at: 436 1466 436
Size: 1030 2392
BbeI G_GCGC'C
Cuts at: 155 856 1104 155
Size: 701 248 2473
Fragments arranged by size:
2473 701 248
BciVI GTATCCnnnnn_n'
Cuts at: 1320 2081 3422 1320
Size: 761 1341 1320
Fragments arranged by size:
1341 1320 761
BclI T'GATC_A
Cuts at: 942 942
Size: 3422
BglI GCCn_nnn'nGGC
Cuts at: 233 233
Size: 3422
BglII A'GATC_T
Cuts at: 937 2974 937
Size: 2037 1385
BlpI GC'TnA_GC
Cuts at: 534 534
Size: 3422
Bme1580I G_kGCm'C
Cuts at: 1047 1140 2196 1047
Size: 93 1056 2273
Fragments arranged by size:
2273 1056 93
BmgBI CAC'GTC
Cuts at: 366 366
Size: 3422
BmrI ACTGGGnnnn_n'
Cuts at: 634 715 1050 3194 634
Size: 81 335 2144 862
Fragments arranged by size:
2144 862 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1789 1789
Size: 3422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 598 1477 1969 2267 364
Size: 234 879 492 298 1519
Fragments arranged by size:
1519 879 492 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 3126 3126
Size: 3422
BsaAI yAC'GTr
Cuts at: 775 775
Size: 3422
BsaBI GATnn'nnATC
Cuts at: 6 936 2973 6
Size: 930 2037 455
Fragments arranged by size:
2037 930 455
BsaHI Gr'CG_yC
Cuts at: 152 236 853 1101 1803 3299 152
Size: 84 617 248 702 1496 275
Fragments arranged by size:
1496 702 617 275 248 84
BsaWI w'CCGG_w
Cuts at: 611 1132 1810 2084 2231 2642 611
Size: 521 678 274 147 411 1391
Fragments arranged by size:
1391 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 847 1605 2182 2678 847
Size: 758 577 496 1591
Fragments arranged by size:
1591 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 964 1010 2218 104
Size: 860 46 1208 1308
Fragments arranged by size:
1308 1208 860 46
BsiHKAI G_wGCw'C
Cuts at: 436 1214 1404 2196 436
Size: 778 190 792 1662
Fragments arranged by size:
1662 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 436 1047 1140 1214 1404 1466 2196 436
Size: 611 93 74 190 62 730 1662
Fragments arranged by size:
1662 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2642 2642
Size: 3422
BspHI T'CATG_A
Cuts at: 2598 2598
Size: 3422
BspMI ACCTGCnnnn'nnnn_
Cuts at: 472 686 988 1369 472
Size: 214 302 381 2525
Fragments arranged by size:
2525 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1714 1768 3252 3417 50
Size: 1664 54 1484 165 55
Fragments arranged by size:
1664 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1334 1334
Size: 3422
BsrFI r'CCGG_y
Cuts at: 1420 1601 1810 1420
Size: 181 209 3032
Fragments arranged by size:
3032 209 181
BssSI C'ACGA_G
Cuts at: 1693 2051 1693
Size: 358 3064
BstAPI GCAn_nnn'nTGC
Cuts at: 799 799
Size: 3422
BstBI TT'CG_AA
Cuts at: 1783 1783
Size: 3422
BstXI CCAn_nnnn'nTGG
Cuts at: 366 366
Size: 3422
BstYI r'GATC_y
Cuts at: 1 447 914 937 1272 1518 2530 2616 2628
Size: 446 467 23 335 246 1012 86 12
Cuts at: 2628 2974 1
Size: 346 449
Fragments arranged by size:
1012 467 449 446 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 117 228 359 117
Size: 111 131 3180
Fragments arranged by size:
3180 131 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 326 1372 1553 1716 326
Size: 1046 181 163 2032
Fragments arranged by size:
2032 1046 181 163
BtsI GCAGTG_nn'
Cuts at: 665 665
Size: 3422
DraI TTT'AAA
Cuts at: 421 421
Size: 3422
DrdI GACnn_nn'nnGTC
Cuts at: 1128 1986 1128
Size: 858 2564
EaeI y'GGCC_r
Cuts at: 114 1007 1181 1572 1599 2944 114
Size: 893 174 391 27 1345 592
Fragments arranged by size:
1345 893 592 391 174 27
EagI C'GGCC_G
Cuts at: 1007 1007
Size: 3422
EarI CTCTTCn'nnn_
Cuts at: 1445 1655 1445
Size: 210 3212
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 143 202 1630 1940 2086 3232 143
Size: 59 1428 310 146 1146 333
Fragments arranged by size:
1428 1146 333 310 146 59
EcoICRI GAG'CTC
Cuts at: 434 434
Size: 3422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1247 1679 2426 2694 1247
Size: 432 747 268 1975
Fragments arranged by size:
1975 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 561 561
Size: 3422
EcoRI G'AATT_C
Cuts at: 426 426
Size: 3422
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1441 1441
Size: 3422
FspI TGC'GCA
Cuts at: 1203 1203
Size: 3422
HaeII r_GCGC'y
Cuts at: 155 387 856 1104 2126 3105 155
Size: 232 469 248 1022 979 472
Fragments arranged by size:
1022 979 472 469 248 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1264 1294 1296 1326 1264
Size: 30 2 30 3360
Fragments arranged by size:
3360 30 30 2
HincII GTy'rAC
Cuts at: 461 461
Size: 3422
KasI G'GCGC_C
Cuts at: 151 852 1100 151
Size: 701 248 2473
Fragments arranged by size:
2473 701 248
KpnI G_GTAC'C
Cuts at: 442 442
Size: 3422
MluI A'CGCG_T
Cuts at: 2855 2855
Size: 3422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2093 2277 2093
Size: 184 3238
MscI TGG'CCA
Cuts at: 1183 1183
Size: 3422
MslI CAynn'nnrTG
Cuts at: 358 364 1411 1538 358
Size: 6 1047 127 2242
Fragments arranged by size:
2242 1047 127 6
MspA1I CmG'CkG
Cuts at: 119 230 533 847 1207 2220 2465 119
Size: 111 303 314 360 1013 245 1076
Fragments arranged by size:
1076 1013 360 314 303 245 111
NaeI GCC'GGC
Cuts at: 1603 1603
Size: 3422
NarI GG'CG_CC
Cuts at: 152 853 1101 152
Size: 701 248 2473
Fragments arranged by size:
2473 701 248
NcoI C'CATG_G
Cuts at: 359 359
Size: 3422
NgoMIV G'CCGG_C
Cuts at: 1601 1601
Size: 3422
NspI r_CATG'y
Cuts at: 475 975 1882 475
Size: 500 907 2015
Fragments arranged by size:
2015 907 500
PciI A'CATG_T
Cuts at: 1878 1878
Size: 3422
PfoI T'CCnGG_A
Cuts at: 293 293
Size: 3422
PmeI GTTT'AAAC
Cuts at: 421 421
Size: 3422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2561 2593 2561
Size: 32 3390
PstI C_TGCA'G
Cuts at: 469 1154 469
Size: 685 2737
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3422
PvuII CAG'CTG
Cuts at: 847 1207 847
Size: 360 3062
RsrII CG'GwC_CG
Cuts at: 1617 1617
Size: 3422
SacI G_AGCT'C
Cuts at: 436 436
Size: 3422
SacII CC_GC'GG
Cuts at: 120 231 120
Size: 111 3311
SalI G'TCGA_C
Cuts at: 459 459
Size: 3422
SapI GCTCTTCn'nnn_
Cuts at: 1445 1655 1445
Size: 210 3212
SbfI CC_TGCA'GG
Cuts at: 469 469
Size: 3422
ScaI AGT'ACT
Cuts at: 2939 2939
Size: 3422
SfcI C'TryA_G
Cuts at: 33 465 1150 2143 2334 33
Size: 432 685 993 191 1121
Fragments arranged by size:
1121 993 685 432 191
SfoI GGC'GCC
Cuts at: 153 854 1102 153
Size: 701 248 2473
Fragments arranged by size:
2473 701 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3422
SmaI CCC'GGG
Cuts at: 444 444
Size: 3422
SmlI C'TyrA_G
Cuts at: 379 577 1492 1984 2246 379
Size: 198 915 492 262 1555
Fragments arranged by size:
1555 915 492 262 198
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3422
SphI G_CATG'C
Cuts at: 475 975 475
Size: 500 2922
StyI C'CwwG_G
Cuts at: 189 359 556 910 189
Size: 170 197 354 2701
Fragments arranged by size:
2701 354 197 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1005 1138 1597 1806 1005
Size: 133 459 209 2621
Fragments arranged by size:
2621 459 209 133
TatI w'GTAC_w
Cuts at: 2937 2937
Size: 3422
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2939 2971 2939
Size: 32 3390
Tth111I GACn'n_nGTC
Cuts at: 1219 1219
Size: 3422
XbaI T'CTAG_A
Cuts at: 453 453
Size: 3422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 917 917
Size: 3422
XmaI C'CCGG_G
Cuts at: 442 442
Size: 3422
XmnI GAAnn'nnTTC
Cuts at: 289 289
Size: 3422
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI