Restriction map for
pF1KE0272
(Circular) (Six-base) MAPSORT of: pF1KE0272.seq Check: 757 from: 1 to: 3740
pF1KE0272 3740 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 180 180
Size: 3740
AccI GT'mk_AC
Cuts at: 778 778
Size: 3740
Acc65I G'GTAC_C
Cuts at: 756 756
Size: 3740
AclI AA'CG_TT
Cuts at: 3580 3580
Size: 3740
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1565 1997 2744 3012 1565
Size: 432 747 268 2293
Fragments arranged by size:
2293 747 432 268
AflIII A'CryG_T
Cuts at: 1092 2196 3173 1092
Size: 1104 977 1659
Fragments arranged by size:
1659 1104 977
AgeI A'CCGG_T
Cuts at: 2128 2128
Size: 3740
AleI CACnn'nnGTG
Cuts at: 676 676
Size: 3740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 508 508
Size: 3740
AlwNI CAG_nnn'CTG
Cuts at: 1045 2612 1045
Size: 1567 2173
ApaI G_GGCC'C
Cuts at: 697 697
Size: 3740
ApaLI G'TGCA_C
Cuts at: 2510 2510
Size: 3740
ApoI r'AATT_y
Cuts at: 744 3093 3349 744
Size: 2349 256 1135
Fragments arranged by size:
2349 1135 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3740
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
AvaI C'yCGr_G
Cuts at: 113 657 760 113
Size: 544 103 3093
Fragments arranged by size:
3093 544 103
BamHI G'GATC_C
Cuts at: 765 765
Size: 3740
BanI G'GyrC_C
Cuts at: 727 756 1170 1418 1453 727
Size: 29 414 248 35 3014
Fragments arranged by size:
3014 414 248 35 29
BanII G_rGCy'C
Cuts at: 281 697 754 1784 281
Size: 416 57 1030 2237
Fragments arranged by size:
2237 1030 416 57
BbeI G_GCGC'C
Cuts at: 1174 1422 1174
Size: 248 3492
BciVI GTATCCnnnnn_n'
Cuts at: 1638 2399 3740 1638
Size: 761 1341 1638
Fragments arranged by size:
1638 1341 761
BclI T'GATC_A
Cuts at: 1260 1260
Size: 3740
BglII A'GATC_T
Cuts at: 1255 3292 1255
Size: 2037 1703
BlpI GC'TnA_GC
Cuts at: 852 852
Size: 3740
Bme1580I G_kGCm'C
Cuts at: 241 683 697 732 1365 1458 2514 241
Size: 442 14 35 633 93 1056 1467
Fragments arranged by size:
1467 1056 633 442 93 35 14
BmgBI CAC'GTC
Cuts at: 405 405
Size: 3740
BmrI ACTGGGnnnn_n'
Cuts at: 490 952 1033 1368 3512 490
Size: 462 81 335 2144 718
Fragments arranged by size:
2144 718 462 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 3740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 659 276
Size: 383 3357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1795 2287 2585 916
Size: 879 492 298 2071
Fragments arranged by size:
2071 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 661 3444 661
Size: 2783 957
BsaAI yAC'GTr
Cuts at: 273 1093 273
Size: 820 2920
BsaBI GATnn'nnATC
Cuts at: 6 1254 3291 6
Size: 1248 2037 455
Fragments arranged by size:
2037 1248 455
BsaHI Gr'CG_yC
Cuts at: 1171 1419 2121 3617 1171
Size: 248 702 1496 1294
Fragments arranged by size:
1496 1294 702 248
BsaWI w'CCGG_w
Cuts at: 335 350 929 1450 2128 2402 2549 2960 335
Size: 15 579 521 678 274 147 411 1115
Fragments arranged by size:
1115 678 579 521 411 274 147 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 508 538 508
Size: 30 3710
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 524 524
Size: 3740
BseYI C'CCAG_C
Cuts at: 198 368 637 1165 1923 2500 2996 198
Size: 170 269 528 758 577 496 942
Fragments arranged by size:
942 758 577 528 496 269 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 490
Size: 3740
BsiEI CG_ry'CG
Cuts at: 104 1282 1328 2536 104
Size: 1178 46 1208 1308
Fragments arranged by size:
1308 1208 1178 46
BsiHKAI G_wGCw'C
Cuts at: 754 1532 1722 2514 754
Size: 778 190 792 1980
Fragments arranged by size:
1980 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 241 281 683 697 732 754 1365 1458 1532
Size: 40 402 14 35 22 611 93 74
Cuts at: 1532 1722 1784 2514 241
Size: 190 62 730 1467
Fragments arranged by size:
1467 730 611 402 190 93 74 62
40 35 22 14
BspEI T'CCGG_A
Cuts at: 350 2960 350
Size: 2610 1130
BspHI T'CATG_A
Cuts at: 2916 2916
Size: 3740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 180 790 1004 1306 1687 180
Size: 610 214 302 381 2233
Fragments arranged by size:
2233 610 381 302 214
BsrBI CCG'CTC
Cuts at: 50 156 165 174 2032 2086 3570 3735 50
Size: 106 9 9 1858 54 1484 165 55
Fragments arranged by size:
1858 1484 165 106 55 54 9 9
BsrDI GCAATG_nn'
Cuts at: 1652 1652
Size: 3740
BsrFI r'CCGG_y
Cuts at: 1738 1919 2128 1738
Size: 181 209 3350
Fragments arranged by size:
3350 209 181
BsrGI T'GTAC_A
Cuts at: 438 551 438
Size: 113 3627
BssSI C'ACGA_G
Cuts at: 2011 2369 2011
Size: 358 3382
BstAPI GCAn_nnn'nTGC
Cuts at: 1117 1117
Size: 3740
BstBI TT'CG_AA
Cuts at: 2101 2101
Size: 3740
BstYI r'GATC_y
Cuts at: 1 765 1232 1255 1590 1836 2848 2934 2946
Size: 764 467 23 335 246 1012 86 12
Cuts at: 2946 3292 1
Size: 346 449
Fragments arranged by size:
1012 764 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 132 527 107
Size: 25 395 3320
Fragments arranged by size:
3320 395 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1690 1871 2034 1690
Size: 181 163 3396
Fragments arranged by size:
3396 181 163
BtsI GCAGTG_nn'
Cuts at: 983 983
Size: 3740
DraI TTT'AAA
Cuts at: 739 739
Size: 3740
DrdI GACnn_nn'nnGTC
Cuts at: 409 1446 2304 409
Size: 1037 858 1845
Fragments arranged by size:
1845 1037 858
EaeI y'GGCC_r
Cuts at: 125 596 1325 1499 1890 1917 3262 125
Size: 471 729 174 391 27 1345 603
Fragments arranged by size:
1345 729 603 471 391 174 27
EagI C'GGCC_G
Cuts at: 1325 1325
Size: 3740
EarI CTCTTCn'nnn_
Cuts at: 1763 1973 1763
Size: 210 3530
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1948 2258 2404 3550 1948
Size: 310 146 1146 2138
Fragments arranged by size:
2138 1146 310 146
EcoICRI GAG'CTC
Cuts at: 752 752
Size: 3740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 659 1565 1997 2744 3012 276
Size: 383 906 432 747 268 1004
Fragments arranged by size:
1004 906 747 432 383 268
EcoO109I rG'GnC_Cy
Cuts at: 653 694 879 653
Size: 41 185 3514
Fragments arranged by size:
3514 185 41
EcoRI G'AATT_C
Cuts at: 744 744
Size: 3740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1759 1759
Size: 3740
FspI TGC'GCA
Cuts at: 1521 1521
Size: 3740
HaeII r_GCGC'y
Cuts at: 199 1174 1422 2444 3423 199
Size: 975 248 1022 979 516
Fragments arranged by size:
1022 979 975 516 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 508 540 608 640 1582 1612 1614 1644 508
Size: 32 68 32 942 30 2 30 2604
Fragments arranged by size:
2604 942 68 32 32 30 30 2
HincII GTy'rAC
Cuts at: 779 779
Size: 3740
KasI G'GCGC_C
Cuts at: 1170 1418 1170
Size: 248 3492
KpnI G_GTAC'C
Cuts at: 760 760
Size: 3740
MluI A'CGCG_T
Cuts at: 3173 3173
Size: 3740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2411 2595 2411
Size: 184 3556
MscI TGG'CCA
Cuts at: 598 1501 598
Size: 903 2837
MslI CAynn'nnrTG
Cuts at: 418 676 1729 1856 418
Size: 258 1053 127 2302
Fragments arranged by size:
2302 1053 258 127
MspA1I CmG'CkG
Cuts at: 123 134 183 218 254 325 851 1165 1525
Size: 11 49 35 36 71 526 314 360
Cuts at: 1525 2538 2783 123
Size: 1013 245 1080
Fragments arranged by size:
1080 1013 526 360 314 245 71 49
36 35 11
NaeI GCC'GGC
Cuts at: 1921 1921
Size: 3740
NarI GG'CG_CC
Cuts at: 1171 1419 1171
Size: 248 3492
NcoI C'CATG_G
Cuts at: 107 527 107
Size: 420 3320
NgoMIV G'CCGG_C
Cuts at: 1919 1919
Size: 3740
NspI r_CATG'y
Cuts at: 489 793 1293 2200 489
Size: 304 500 907 2029
Fragments arranged by size:
2029 907 500 304
PciI A'CATG_T
Cuts at: 2196 2196
Size: 3740
PmeI GTTT'AAAC
Cuts at: 739 739
Size: 3740
PmlI CAC'GTG
Cuts at: 273 273
Size: 3740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2879 2911 2879
Size: 32 3708
PspOMI G'GGCC_C
Cuts at: 693 693
Size: 3740
PstI C_TGCA'G
Cuts at: 787 1472 787
Size: 685 3055
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3740
PvuII CAG'CTG
Cuts at: 218 325 1165 1525 218
Size: 107 840 360 2433
Fragments arranged by size:
2433 840 360 107
RsrII CG'GwC_CG
Cuts at: 1935 1935
Size: 3740
SacI G_AGCT'C
Cuts at: 754 754
Size: 3740
SacII CC_GC'GG
Cuts at: 135 135
Size: 3740
SalI G'TCGA_C
Cuts at: 777 777
Size: 3740
SapI GCTCTTCn'nnn_
Cuts at: 1763 1973 1763
Size: 210 3530
SbfI CC_TGCA'GG
Cuts at: 787 787
Size: 3740
ScaI AGT'ACT
Cuts at: 3257 3257
Size: 3740
SfcI C'TryA_G
Cuts at: 33 459 543 783 1468 2461 2652 33
Size: 426 84 240 685 993 191 1121
Fragments arranged by size:
1121 993 685 426 240 191 84
SfoI GGC'GCC
Cuts at: 1172 1420 1172
Size: 248 3492
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
SmaI CCC'GGG
Cuts at: 115 762 115
Size: 647 3093
SmlI C'TyrA_G
Cuts at: 895 1810 2302 2564 895
Size: 915 492 262 2071
Fragments arranged by size:
2071 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3740
SphI G_CATG'C
Cuts at: 489 793 1293 489
Size: 304 500 2936
Fragments arranged by size:
2936 500 304
StyI C'CwwG_G
Cuts at: 107 527 648 874 1228 107
Size: 420 121 226 354 2619
Fragments arranged by size:
2619 420 354 226 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1323 1456 1915 2124 1323
Size: 133 459 209 2939
Fragments arranged by size:
2939 459 209 133
TatI w'GTAC_w
Cuts at: 438 551 3255 438
Size: 113 2704 923
Fragments arranged by size:
2704 923 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 3708
Tth111I GACn'n_nGTC
Cuts at: 1537 1537
Size: 3740
XbaI T'CTAG_A
Cuts at: 771 771
Size: 3740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1235 1235
Size: 3740
XmaI C'CCGG_G
Cuts at: 113 760 113
Size: 647 3093
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PpuMI PshAI PsiI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI