Restriction map for
pF1KE0266
(Circular) (Six-base) MAPSORT of: pF1KE0266.seq Check: 7433 from: 1 to: 4463
pF1KE0266 4463 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 893 893
Size: 4463
AatII G_ACGT'C
Cuts at: 785 785
Size: 4463
AccI GT'mk_AC
Cuts at: 1501 1501
Size: 4463
Acc65I G'GTAC_C
Cuts at: 1479 1479
Size: 4463
AclI AA'CG_TT
Cuts at: 4303 4303
Size: 4463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1244 2288 2720 3467 3735 1244
Size: 1044 432 747 268 1972
Fragments arranged by size:
1972 1044 747 432 268
AfeI AGC'GCT
Cuts at: 1348 1348
Size: 4463
AflIII A'CryG_T
Cuts at: 824 1815 2919 3896 824
Size: 991 1104 977 1391
Fragments arranged by size:
1391 1104 991 977
AgeI A'CCGG_T
Cuts at: 2851 2851
Size: 4463
AleI CACnn'nnGTG
Cuts at: 505 505
Size: 4463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 853 853
Size: 4463
AlwNI CAG_nnn'CTG
Cuts at: 270 333 957 1455 1768 3335 270
Size: 63 624 498 313 1567 1398
Fragments arranged by size:
1567 1398 624 498 313 63
ApaI G_GGCC'C
Cuts at: 1122 1252 1122
Size: 130 4333
ApaLI G'TGCA_C
Cuts at: 3233 3233
Size: 4463
ApoI r'AATT_y
Cuts at: 1467 3816 4072 1467
Size: 2349 256 1858
Fragments arranged by size:
2349 1858 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4463
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
AvaI C'yCGr_G
Cuts at: 1483 1483
Size: 4463
BamHI G'GATC_C
Cuts at: 1488 1488
Size: 4463
BanI G'GyrC_C
Cuts at: 526 644 1479 1893 2141 2176 526
Size: 118 835 414 248 35 2813
Fragments arranged by size:
2813 835 414 248 118 35
BanII G_rGCy'C
Cuts at: 116 773 1122 1175 1252 1477 2507 116
Size: 657 349 53 77 225 1030 2072
Fragments arranged by size:
2072 1030 657 349 225 77 53
BbeI G_GCGC'C
Cuts at: 530 1897 2145 530
Size: 1367 248 2848
Fragments arranged by size:
2848 1367 248
BbsI GAAGACnn'nnnn_
Cuts at: 939 939
Size: 4463
BbvCI CC'TCA_GC
Cuts at: 130 182 130
Size: 52 4411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1256 1290 1256
Size: 34 4429
BciVI GTATCCnnnnn_n'
Cuts at: 344 2361 3122 4463 344
Size: 2017 761 1341 344
Fragments arranged by size:
2017 1341 761 344
BclI T'GATC_A
Cuts at: 1983 1983
Size: 4463
BglI GCCn_nnn'nGGC
Cuts at: 524 685 735 1179 524
Size: 161 50 444 3808
Fragments arranged by size:
3808 444 161 50
BglII A'GATC_T
Cuts at: 1978 4015 1978
Size: 2037 2426
BlpI GC'TnA_GC
Cuts at: 1575 1575
Size: 4463
Bme1580I G_kGCm'C
Cuts at: 240 647 1122 1252 2088 2181 3237 240
Size: 407 475 130 836 93 1056 1466
Fragments arranged by size:
1466 1056 836 475 407 130 93
BmrI ACTGGGnnnn_n'
Cuts at: 1082 1093 1209 1675 1756 2091 4235 1082
Size: 11 116 466 81 335 2144 1310
Fragments arranged by size:
2144 1310 466 335 116 81 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 4463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 470 304
Size: 166 4297
Bpu10I CC'TnA_GC
Cuts at: 130 182 130
Size: 52 4411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1639 2518 3010 3308 184
Size: 1455 879 492 298 1339
Fragments arranged by size:
1455 1339 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 237 4167 237
Size: 3930 533
BsaAI yAC'GTr
Cuts at: 825 1302 1816 825
Size: 477 514 3472
Fragments arranged by size:
3472 514 477
BsaBI GATnn'nnATC
Cuts at: 6 1977 4014 6
Size: 1971 2037 455
Fragments arranged by size:
2037 1971 455
BsaHI Gr'CG_yC
Cuts at: 527 599 782 920 1894 2142 2844 4340 527
Size: 72 183 138 974 248 702 1496 650
Fragments arranged by size:
1496 974 702 650 248 183 138 72
BsaWI w'CCGG_w
Cuts at: 116 1652 2173 2851 3125 3272 3683 116
Size: 1536 521 678 274 147 411 896
Fragments arranged by size:
1536 896 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 427 536 602 720 915 1128 1242 1343 1888
Size: 109 66 118 195 213 114 101 545
Cuts at: 1888 2646 3223 3719 427
Size: 758 577 496 1171
Fragments arranged by size:
1171 758 577 545 496 213 195 118
114 109 101 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1279 1468 1279
Size: 189 4274
BsiEI CG_ry'CG
Cuts at: 104 664 983 2005 2051 3259 104
Size: 560 319 1022 46 1208 1308
Fragments arranged by size:
1308 1208 1022 560 319 46
BsiHKAI G_wGCw'C
Cuts at: 749 854 1477 2255 2445 3237 749
Size: 105 623 778 190 792 1975
Fragments arranged by size:
1975 792 778 623 190 105
Bsp1286I G_dGCh'C
Cuts at: 116 240 647 749 773 854 1122 1175 1252
Size: 124 407 102 24 81 268 53 77
Cuts at: 1252 1477 2088 2181 2255 2445 2507 3237 116
Size: 225 611 93 74 190 62 730 1342
Fragments arranged by size:
1342 730 611 407 268 225 190 124
102 93 81 77 74 62 53 24
BspEI T'CCGG_A
Cuts at: 116 3683 116
Size: 3567 896
BspHI T'CATG_A
Cuts at: 1064 1427 3639 1064
Size: 363 2212 1888
Fragments arranged by size:
2212 1888 363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 842 893 1079 1513 1727 2029 2410 842
Size: 51 186 434 214 302 381 2895
Fragments arranged by size:
2895 434 381 302 214 186 51
BsrBI CCG'CTC
Cuts at: 50 873 996 2755 2809 4293 4458 50
Size: 823 123 1759 54 1484 165 55
Fragments arranged by size:
1759 1484 823 165 123 55 54
BsrDI GCAATG_nn'
Cuts at: 1310 2375 1310
Size: 1065 3398
BsrFI r'CCGG_y
Cuts at: 188 546 729 978 2461 2642 2851 188
Size: 358 183 249 1483 181 209 1800
Fragments arranged by size:
1800 1483 358 249 209 183 181
BssSI C'ACGA_G
Cuts at: 564 1258 2734 3092 564
Size: 694 1476 358 1935
Fragments arranged by size:
1935 1476 694 358
BstAPI GCAn_nnn'nTGC
Cuts at: 566 1840 566
Size: 1274 3189
BstBI TT'CG_AA
Cuts at: 2824 2824
Size: 4463
BstXI CCAn_nnnn'nTGG
Cuts at: 1422 1422
Size: 4463
BstYI r'GATC_y
Cuts at: 1 1488 1955 1978 2313 2559 3571 3657 3669
Size: 1487 467 23 335 246 1012 86 12
Cuts at: 3669 4015 1
Size: 346 449
Fragments arranged by size:
1487 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1028 1415 107
Size: 921 387 3155
Fragments arranged by size:
3155 921 387
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2413 2594 2757 2413
Size: 181 163 4119
Fragments arranged by size:
4119 181 163
BtsI GCAGTG_nn'
Cuts at: 606 1706 606
Size: 1100 3363
DraI TTT'AAA
Cuts at: 1462 1462
Size: 4463
DrdI GACnn_nn'nnGTC
Cuts at: 2169 3027 2169
Size: 858 3605
EaeI y'GGCC_r
Cuts at: 445 548 661 962 980 1016 1400 2048 2222
Size: 103 113 301 18 36 384 648 174
Cuts at: 2222 2613 2640 3985 445
Size: 391 27 1345 923
Fragments arranged by size:
1345 923 648 391 384 301 174 113
103 36 27 18
EagI C'GGCC_G
Cuts at: 661 980 2048 661
Size: 319 1068 3076
Fragments arranged by size:
3076 1068 319
EarI CTCTTCn'nnn_
Cuts at: 2486 2696 2486
Size: 210 4253
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 483 703 1084 2671 2981 3127 4273 483
Size: 220 381 1587 310 146 1146 673
Fragments arranged by size:
1587 1146 673 381 310 220 146
EcoICRI GAG'CTC
Cuts at: 1475 1475
Size: 4463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 470 1244 2288 2720 3467 3735 304
Size: 166 774 1044 432 747 268 1032
Fragments arranged by size:
1044 1032 774 747 432 268 166
EcoNI CCTnn'n_nnAGG
Cuts at: 1113 1226 1113
Size: 113 4350
EcoO109I rG'GnC_Cy
Cuts at: 387 1118 1119 1146 1249 1452 1602 387
Size: 731 1 27 103 203 150 3248
Fragments arranged by size:
3248 731 203 150 103 27 1
EcoRI G'AATT_C
Cuts at: 1467 1467
Size: 4463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4463
FspI TGC'GCA
Cuts at: 2244 2244
Size: 4463
HaeII r_GCGC'y
Cuts at: 530 674 940 1350 1371 1897 2145 3167 4146
Size: 144 266 410 21 526 248 1022 979
Cuts at: 4146 530
Size: 847
Fragments arranged by size:
1022 979 847 526 410 266 248 144
21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 327 359 895 927 2305 2335 2337 2367 327
Size: 32 536 32 1378 30 2 30 2423
Fragments arranged by size:
2423 1378 536 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1502 1502
Size: 4463
KasI G'GCGC_C
Cuts at: 526 1893 2141 526
Size: 1367 248 2848
Fragments arranged by size:
2848 1367 248
KpnI G_GTAC'C
Cuts at: 1483 1483
Size: 4463
MluI A'CGCG_T
Cuts at: 3896 3896
Size: 4463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3134 3318 3134
Size: 184 4279
MscI TGG'CCA
Cuts at: 1018 1402 2224 1018
Size: 384 822 3257
Fragments arranged by size:
3257 822 384
MslI CAynn'nnrTG
Cuts at: 310 505 1079 2452 2579 310
Size: 195 574 1373 127 2194
Fragments arranged by size:
2194 1373 574 195 127
MspA1I CmG'CkG
Cuts at: 1246 1574 1888 2248 3261 3506 1246
Size: 328 314 360 1013 245 2203
Fragments arranged by size:
2203 1013 360 328 314 245
NaeI GCC'GGC
Cuts at: 190 548 731 2644 190
Size: 358 183 1913 2009
Fragments arranged by size:
2009 1913 358 183
NarI GG'CG_CC
Cuts at: 527 1894 2142 527
Size: 1367 248 2848
Fragments arranged by size:
2848 1367 248
NcoI C'CATG_G
Cuts at: 107 1028 1415 107
Size: 921 387 3155
Fragments arranged by size:
3155 921 387
NgoMIV G'CCGG_C
Cuts at: 188 546 729 2642 188
Size: 358 183 1913 2009
Fragments arranged by size:
2009 1913 358 183
NotI GC'GGCC_GC
Cuts at: 661 661
Size: 4463
NspI r_CATG'y
Cuts at: 729 975 1516 2016 2923 729
Size: 246 541 500 907 2269
Fragments arranged by size:
2269 907 541 500 246
PciI A'CATG_T
Cuts at: 2919 2919
Size: 4463
PfoI T'CCnGG_A
Cuts at: 628 628
Size: 4463
PmeI GTTT'AAAC
Cuts at: 1462 1462
Size: 4463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 3602
Size: 32 4431
PpuMI rG'GwC_Cy
Cuts at: 387 387
Size: 4463
PshAI GACnn'nnGTC
Cuts at: 1030 1030
Size: 4463
PspOMI G'GGCC_C
Cuts at: 1118 1248 1118
Size: 130 4333
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1060 1092 1060
Size: 32 4431
PstI C_TGCA'G
Cuts at: 168 678 1510 2195 168
Size: 510 832 685 2436
Fragments arranged by size:
2436 832 685 510
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4463
PvuII CAG'CTG
Cuts at: 1888 2248 1888
Size: 360 4103
RsrII CG'GwC_CG
Cuts at: 2658 2658
Size: 4463
SacI G_AGCT'C
Cuts at: 1477 1477
Size: 4463
SalI G'TCGA_C
Cuts at: 1500 1500
Size: 4463
SanDI GG'GwC_CC
Cuts at: 387 387
Size: 4463
SapI GCTCTTCn'nnn_
Cuts at: 2486 2696 2486
Size: 210 4253
SbfI CC_TGCA'GG
Cuts at: 1510 1510
Size: 4463
ScaI AGT'ACT
Cuts at: 1009 3980 1009
Size: 2971 1492
SexAI A'CCwGG_T
Cuts at: 326 326
Size: 4463
SfcI C'TryA_G
Cuts at: 33 164 674 1506 2191 3184 3375 33
Size: 131 510 832 685 993 191 1121
Fragments arranged by size:
1121 993 832 685 510 191 131
SfoI GGC'GCC
Cuts at: 528 1895 2143 528
Size: 1367 248 2848
Fragments arranged by size:
2848 1367 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
SmaI CCC'GGG
Cuts at: 1485 1485
Size: 4463
SmlI C'TyrA_G
Cuts at: 199 1618 2533 3025 3287 199
Size: 1419 915 492 262 1375
Fragments arranged by size:
1419 1375 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4463
SphI G_CATG'C
Cuts at: 729 975 1516 2016 729
Size: 246 541 500 3176
Fragments arranged by size:
3176 541 500 246
StyI C'CwwG_G
Cuts at: 107 1028 1415 1597 1951 107
Size: 921 387 182 354 2619
Fragments arranged by size:
2619 921 387 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2046 2179 2638 2847 2046
Size: 133 459 209 3662
Fragments arranged by size:
3662 459 209 133
TatI w'GTAC_w
Cuts at: 1007 3978 1007
Size: 2971 1492
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1040 1072 3980 4012 1040
Size: 32 2908 32 1491
Fragments arranged by size:
2908 1491 32 32
Tth111I GACn'n_nGTC
Cuts at: 294 2260 294
Size: 1966 2497
XbaI T'CTAG_A
Cuts at: 1494 1494
Size: 4463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 504 1026 1106 1958 504
Size: 522 80 852 3009
Fragments arranged by size:
3009 852 522 80
XmaI C'CCGG_G
Cuts at: 1483 1483
Size: 4463
ZraI GAC'GTC
Cuts at: 783 783
Size: 4463
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PciI PfoI PmeI PpiI PpuMI PshAI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BfrBI BmgBI
BmtI BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI
PacI PasI PflMI PmlI PsiI PspXI SacII SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI