Restriction map for
pF1KE0265
(Circular) (Six-base) MAPSORT of: pF1KE0265.seq Check: 4380 from: 1 to: 3503
pF1KE0265 3503 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 257 541 257
Size: 284 3219
Acc65I G'GTAC_C
Cuts at: 519 519
Size: 3503
AclI AA'CG_TT
Cuts at: 3343 3343
Size: 3503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 1328 1760 2507 2775 163
Size: 1165 432 747 268 891
Fragments arranged by size:
1165 891 747 432 268
AflIII A'CryG_T
Cuts at: 855 1959 2936 855
Size: 1104 977 1422
Fragments arranged by size:
1422 1104 977
AgeI A'CCGG_T
Cuts at: 1891 1891
Size: 3503
AhdI GACnn_n'nnGTC
Cuts at: 473 473
Size: 3503
AlwNI CAG_nnn'CTG
Cuts at: 808 2375 808
Size: 1567 1936
ApaLI G'TGCA_C
Cuts at: 2273 2273
Size: 3503
ApoI r'AATT_y
Cuts at: 507 2856 3112 507
Size: 2349 256 898
Fragments arranged by size:
2349 898 256
AscI GG'CGCG_CC
Cuts at: 248 248
Size: 3503
AseI AT'TA_AT
Cuts at: 20 20
Size: 3503
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3503
AvaI C'yCGr_G
Cuts at: 274 320 523 274
Size: 46 203 3254
Fragments arranged by size:
3254 203 46
BamHI G'GATC_C
Cuts at: 528 528
Size: 3503
BanI G'GyrC_C
Cuts at: 519 933 1181 1216 519
Size: 414 248 35 2806
Fragments arranged by size:
2806 414 248 35
BanII G_rGCy'C
Cuts at: 517 1547 517
Size: 1030 2473
BbeI G_GCGC'C
Cuts at: 937 1185 937
Size: 248 3255
BbsI GAAGACnn'nnnn_
Cuts at: 340 472 340
Size: 132 3371
BciVI GTATCCnnnnn_n'
Cuts at: 1401 2162 3503 1401
Size: 761 1341 1401
Fragments arranged by size:
1401 1341 761
BclI T'GATC_A
Cuts at: 1023 1023
Size: 3503
BglII A'GATC_T
Cuts at: 1018 3055 1018
Size: 2037 1466
BlpI GC'TnA_GC
Cuts at: 615 615
Size: 3503
Bme1580I G_kGCm'C
Cuts at: 1128 1221 2277 1128
Size: 93 1056 2354
Fragments arranged by size:
2354 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 715 796 1131 3275 715
Size: 81 335 2144 943
Fragments arranged by size:
2144 943 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1870 1870
Size: 3503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 343 319
Size: 24 3479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1558 2050 2348 679
Size: 879 492 298 1834
Fragments arranged by size:
1834 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3207 3207
Size: 3503
BsaAI yAC'GTr
Cuts at: 856 856
Size: 3503
BsaBI GATnn'nnATC
Cuts at: 6 1017 3054 6
Size: 1011 2037 455
Fragments arranged by size:
2037 1011 455
BsaHI Gr'CG_yC
Cuts at: 428 934 1182 1884 3380 428
Size: 506 248 702 1496 551
Fragments arranged by size:
1496 702 551 506 248
BsaWI w'CCGG_w
Cuts at: 692 1213 1891 2165 2312 2723 692
Size: 521 678 274 147 411 1472
Fragments arranged by size:
1472 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 399 928 1686 2263 2759 399
Size: 529 758 577 496 1143
Fragments arranged by size:
1143 758 577 529 496
BsiEI CG_ry'CG
Cuts at: 104 1045 1091 2299 104
Size: 941 46 1208 1308
Fragments arranged by size:
1308 1208 941 46
BsiHKAI G_wGCw'C
Cuts at: 517 1295 1485 2277 517
Size: 778 190 792 1743
Fragments arranged by size:
1743 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 517 1128 1221 1295 1485 1547 2277 517
Size: 611 93 74 190 62 730 1743
Fragments arranged by size:
1743 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2723 2723
Size: 3503
BspHI T'CATG_A
Cuts at: 2679 2679
Size: 3503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 553 767 1069 1450 553
Size: 214 302 381 2606
Fragments arranged by size:
2606 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1795 1849 3333 3498 50
Size: 1745 54 1484 165 55
Fragments arranged by size:
1745 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1415 1415
Size: 3503
BsrFI r'CCGG_y
Cuts at: 244 1501 1682 1891 244
Size: 1257 181 209 1856
Fragments arranged by size:
1856 1257 209 181
BssHII G'CGCG_C
Cuts at: 248 248
Size: 3503
BssSI C'ACGA_G
Cuts at: 1774 2132 1774
Size: 358 3145
BstAPI GCAn_nnn'nTGC
Cuts at: 207 880 207
Size: 673 2830
BstBI TT'CG_AA
Cuts at: 1864 1864
Size: 3503
BstYI r'GATC_y
Cuts at: 1 293 528 995 1018 1353 1599 2611 2697
Size: 292 235 467 23 335 246 1012 86
Cuts at: 2697 2709 3055 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 292 246 235
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 187 399 1453 1634 1797 187
Size: 212 1054 181 163 1893
Fragments arranged by size:
1893 1054 212 181 163
BtsI GCAGTG_nn'
Cuts at: 746 746
Size: 3503
DraI TTT'AAA
Cuts at: 502 502
Size: 3503
DrdI GACnn_nn'nnGTC
Cuts at: 1209 2067 1209
Size: 858 2645
EaeI y'GGCC_r
Cuts at: 365 1088 1262 1653 1680 3025 365
Size: 723 174 391 27 1345 843
Fragments arranged by size:
1345 843 723 391 174 27
EagI C'GGCC_G
Cuts at: 1088 1088
Size: 3503
EarI CTCTTCn'nnn_
Cuts at: 1526 1736 1526
Size: 210 3293
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1711 2021 2167 3313 1711
Size: 310 146 1146 1901
Fragments arranged by size:
1901 1146 310 146
EcoICRI GAG'CTC
Cuts at: 515 515
Size: 3503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 319 343 1328 1760 2507 2775 163
Size: 156 24 985 432 747 268 891
Fragments arranged by size:
985 891 747 432 268 156 24
EcoO109I rG'GnC_Cy
Cuts at: 642 642
Size: 3503
EcoRI G'AATT_C
Cuts at: 507 507
Size: 3503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1522 1522
Size: 3503
FspI TGC'GCA
Cuts at: 214 1284 214
Size: 1070 2433
HaeII r_GCGC'y
Cuts at: 937 1185 2207 3186 937
Size: 248 1022 979 1254
Fragments arranged by size:
1254 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 459 491 1345 1375 1377 1407 459
Size: 32 854 30 2 30 2555
Fragments arranged by size:
2555 854 32 30 30 2
HincII GTy'rAC
Cuts at: 542 542
Size: 3503
KasI G'GCGC_C
Cuts at: 933 1181 933
Size: 248 3255
KpnI G_GTAC'C
Cuts at: 523 523
Size: 3503
MluI A'CGCG_T
Cuts at: 2936 2936
Size: 3503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2358 2174
Size: 184 3319
MscI TGG'CCA
Cuts at: 367 1264 367
Size: 897 2606
MslI CAynn'nnrTG
Cuts at: 1492 1619 1492
Size: 127 3376
MspA1I CmG'CkG
Cuts at: 363 614 928 1288 2301 2546 363
Size: 251 314 360 1013 245 1320
Fragments arranged by size:
1320 1013 360 314 251 245
NaeI GCC'GGC
Cuts at: 246 1684 246
Size: 1438 2065
NarI GG'CG_CC
Cuts at: 934 1182 934
Size: 248 3255
NgoMIV G'CCGG_C
Cuts at: 244 1682 244
Size: 1438 2065
NspI r_CATG'y
Cuts at: 556 1056 1963 556
Size: 500 907 2096
Fragments arranged by size:
2096 907 500
PasI CC'CwG_GG
Cuts at: 420 420
Size: 3503
PciI A'CATG_T
Cuts at: 1959 1959
Size: 3503
PfoI T'CCnGG_A
Cuts at: 296 296
Size: 3503
PmeI GTTT'AAAC
Cuts at: 502 502
Size: 3503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2642 2674 2642
Size: 32 3471
PspXI vC'TCGA_Gb
Cuts at: 274 274
Size: 3503
PstI C_TGCA'G
Cuts at: 550 1235 550
Size: 685 2818
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3503
PvuII CAG'CTG
Cuts at: 363 928 1288 363
Size: 565 360 2578
Fragments arranged by size:
2578 565 360
RsrII CG'GwC_CG
Cuts at: 1698 1698
Size: 3503
SacI G_AGCT'C
Cuts at: 517 517
Size: 3503
SalI G'TCGA_C
Cuts at: 540 540
Size: 3503
SapI GCTCTTCn'nnn_
Cuts at: 1526 1736 1526
Size: 210 3293
SbfI CC_TGCA'GG
Cuts at: 550 550
Size: 3503
ScaI AGT'ACT
Cuts at: 3020 3020
Size: 3503
SfcI C'TryA_G
Cuts at: 33 546 1231 2224 2415 33
Size: 513 685 993 191 1121
Fragments arranged by size:
1121 993 685 513 191
SfoI GGC'GCC
Cuts at: 935 1183 935
Size: 248 3255
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3503
SmaI CCC'GGG
Cuts at: 525 525
Size: 3503
SmlI C'TyrA_G
Cuts at: 274 658 1573 2065 2327 274
Size: 384 915 492 262 1450
Fragments arranged by size:
1450 915 492 384 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3503
SphI G_CATG'C
Cuts at: 556 1056 556
Size: 500 3003
StyI C'CwwG_G
Cuts at: 146 637 991 146
Size: 491 354 2658
Fragments arranged by size:
2658 491 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1086 1219 1678 1887 1086
Size: 133 459 209 2702
Fragments arranged by size:
2702 459 209 133
TatI w'GTAC_w
Cuts at: 3018 3018
Size: 3503
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3020 3052 3020
Size: 32 3471
Tth111I GACn'n_nGTC
Cuts at: 1300 1300
Size: 3503
XbaI T'CTAG_A
Cuts at: 534 534
Size: 3503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 998 998
Size: 3503
XhoI C'TCGA_G
Cuts at: 274 274
Size: 3503
XmaI C'CCGG_G
Cuts at: 523 523
Size: 3503
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PshAI PsiI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI