Restriction map for
pF1KE0255
(Circular) (Six-base) MAPSORT of: pF1KE0255.seq Check: 6502 from: 1 to: 4379
pF1KE0255 4379 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 954 954
Size: 4379
AccI GT'mk_AC
Cuts at: 1417 1417
Size: 4379
Acc65I G'GTAC_C
Cuts at: 1395 1395
Size: 4379
AclI AA'CG_TT
Cuts at: 4219 4219
Size: 4379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 1214 2204 2636 3383 3651 461
Size: 753 990 432 747 268 1189
Fragments arranged by size:
1189 990 753 747 432 268
AflIII A'CryG_T
Cuts at: 713 1731 2835 3812 713
Size: 1018 1104 977 1280
Fragments arranged by size:
1280 1104 1018 977
AgeI A'CCGG_T
Cuts at: 2767 2767
Size: 4379
AlwNI CAG_nnn'CTG
Cuts at: 625 993 1684 3251 625
Size: 368 691 1567 1753
Fragments arranged by size:
1753 1567 691 368
ApaI G_GGCC'C
Cuts at: 381 381
Size: 4379
ApaLI G'TGCA_C
Cuts at: 3149 3149
Size: 4379
ApoI r'AATT_y
Cuts at: 1383 3732 3988 1383
Size: 2349 256 1774
Fragments arranged by size:
2349 1774 256
AseI AT'TA_AT
Cuts at: 20 647 20
Size: 627 3752
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
AvaI C'yCGr_G
Cuts at: 948 1399 948
Size: 451 3928
BamHI G'GATC_C
Cuts at: 143 1404 143
Size: 1261 3118
BanI G'GyrC_C
Cuts at: 1395 1809 2057 2092 1395
Size: 414 248 35 3682
Fragments arranged by size:
3682 414 248 35
BanII G_rGCy'C
Cuts at: 381 1393 2423 381
Size: 1012 1030 2337
Fragments arranged by size:
2337 1030 1012
BbeI G_GCGC'C
Cuts at: 1813 2061 1813
Size: 248 4131
BbsI GAAGACnn'nnnn_
Cuts at: 1085 1085
Size: 4379
BbvCI CC'TCA_GC
Cuts at: 393 393
Size: 4379
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3038 4379 2277
Size: 761 1341 2277
Fragments arranged by size:
2277 1341 761
BclI T'GATC_A
Cuts at: 1899 1899
Size: 4379
BglII A'GATC_T
Cuts at: 1894 3931 1894
Size: 2037 2342
BlpI GC'TnA_GC
Cuts at: 1491 1491
Size: 4379
Bme1580I G_kGCm'C
Cuts at: 381 2004 2097 3153 381
Size: 1623 93 1056 1607
Fragments arranged by size:
1623 1607 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 142 644 1016 1154 1357 1591 1672 2007 4151
Size: 502 372 138 203 234 81 335 2144
Cuts at: 4151 142
Size: 370
Fragments arranged by size:
2144 502 372 370 335 234 203 138
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 839 2746 839
Size: 1907 2472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 811 883 427
Size: 384 72 3923
Fragments arranged by size:
3923 384 72
Bpu10I CC'TnA_GC
Cuts at: 393 393
Size: 4379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 2434 2926 3224 1555
Size: 879 492 298 2710
Fragments arranged by size:
2710 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1105 4083 1105
Size: 2978 1401
BsaAI yAC'GTr
Cuts at: 1732 1732
Size: 4379
BsaBI GATnn'nnATC
Cuts at: 6 1893 3930 6
Size: 1887 2037 455
Fragments arranged by size:
2037 1887 455
BsaHI Gr'CG_yC
Cuts at: 311 1810 2058 2760 4256 311
Size: 1499 248 702 1496 434
Fragments arranged by size:
1499 1496 702 434 248
BsaWI w'CCGG_w
Cuts at: 464 1568 2089 2767 3041 3188 3599 464
Size: 1104 521 678 274 147 411 1244
Fragments arranged by size:
1244 1104 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 463 1228 463
Size: 765 3614
BseYI C'CCAG_C
Cuts at: 493 1244 1804 2562 3139 3635 493
Size: 751 560 758 577 496 1237
Fragments arranged by size:
1237 758 751 577 560 496
BsiEI CG_ry'CG
Cuts at: 104 1921 1967 3175 104
Size: 1817 46 1208 1308
Fragments arranged by size:
1817 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1393 2171 2361 3153 1393
Size: 778 190 792 2619
Fragments arranged by size:
2619 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 381 1393 2004 2097 2171 2361 2423 3153 381
Size: 1012 611 93 74 190 62 730 1607
Fragments arranged by size:
1607 1012 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 464 3599 464
Size: 3135 1244
BspHI T'CATG_A
Cuts at: 3555 3555
Size: 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 954 1429 1643 1945 2326 954
Size: 475 214 302 381 3007
Fragments arranged by size:
3007 475 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2671 2725 4209 4374 50
Size: 2621 54 1484 165 55
Fragments arranged by size:
2621 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2291 2291
Size: 4379
BsrFI r'CCGG_y
Cuts at: 2377 2558 2767 2377
Size: 181 209 3989
Fragments arranged by size:
3989 209 181
BssSI C'ACGA_G
Cuts at: 2650 3008 2650
Size: 358 4021
BstAPI GCAn_nnn'nTGC
Cuts at: 1756 1756
Size: 4379
BstBI TT'CG_AA
Cuts at: 2740 2740
Size: 4379
BstEII G'GTnAC_C
Cuts at: 1320 1320
Size: 4379
BstXI CCAn_nnnn'nTGG
Cuts at: 357 1029 357
Size: 672 3707
BstYI r'GATC_y
Cuts at: 1 143 1404 1871 1894 2229 2475 3487 3573
Size: 142 1261 467 23 335 246 1012 86
Cuts at: 3573 3585 3931 1
Size: 12 346 449
Fragments arranged by size:
1261 1012 467 449 346 335 246 142
86 23 12
BtgI C'CryG_G
Cuts at: 107 180 107
Size: 73 4306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1201 2329 2510 2673 1201
Size: 1128 181 163 2907
Fragments arranged by size:
2907 1128 181 163
BtsI GCAGTG_nn'
Cuts at: 1622 1622
Size: 4379
DraI TTT'AAA
Cuts at: 1378 1378
Size: 4379
DrdI GACnn_nn'nnGTC
Cuts at: 2085 2943 2085
Size: 858 3521
EaeI y'GGCC_r
Cuts at: 1964 2138 2529 2556 3901 1964
Size: 174 391 27 1345 2442
Fragments arranged by size:
2442 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1964 1964
Size: 4379
EarI CTCTTCn'nnn_
Cuts at: 1345 2402 2612 1345
Size: 1057 210 3112
Fragments arranged by size:
3112 1057 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2587 2897 3043 4189 2587
Size: 310 146 1146 2777
Fragments arranged by size:
2777 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1391 1391
Size: 4379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 461 811 883 1214 2204 2636 3383 3651
Size: 34 350 72 331 990 432 747 268
Cuts at: 3651 427
Size: 1155
Fragments arranged by size:
1155 990 747 432 350 331 268 72
34
EcoNI CCTnn'n_nnAGG
Cuts at: 1315 1315
Size: 4379
EcoO109I rG'GnC_Cy
Cuts at: 202 377 411 831 1518 202
Size: 175 34 420 687 3063
Fragments arranged by size:
3063 687 420 175 34
EcoRI G'AATT_C
Cuts at: 1383 1383
Size: 4379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1102 2398 1102
Size: 1296 3083
FspI TGC'GCA
Cuts at: 2160 2160
Size: 4379
HaeII r_GCGC'y
Cuts at: 931 1813 2061 3083 4062 931
Size: 882 248 1022 979 1248
Fragments arranged by size:
1248 1022 979 882 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 410 442 2221 2251 2253 2283 410
Size: 32 1779 30 2 30 2506
Fragments arranged by size:
2506 1779 32 30 30 2
HincII GTy'rAC
Cuts at: 1359 1418 1359
Size: 59 4320
KasI G'GCGC_C
Cuts at: 1809 2057 1809
Size: 248 4131
KpnI G_GTAC'C
Cuts at: 1399 1399
Size: 4379
MfeI C'AATT_G
Cuts at: 1009 1009
Size: 4379
MluI A'CGCG_T
Cuts at: 3812 3812
Size: 4379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1010 3050 3234 1010
Size: 2040 184 2155
Fragments arranged by size:
2155 2040 184
MscI TGG'CCA
Cuts at: 2140 2140
Size: 4379
MslI CAynn'nnrTG
Cuts at: 718 2368 2495 718
Size: 1650 127 2602
Fragments arranged by size:
2602 1650 127
MspA1I CmG'CkG
Cuts at: 1490 1804 2164 3177 3422 1490
Size: 314 360 1013 245 2447
Fragments arranged by size:
2447 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2560 2560
Size: 4379
NarI GG'CG_CC
Cuts at: 1810 2058 1810
Size: 248 4131
NcoI C'CATG_G
Cuts at: 107 180 107
Size: 73 4306
NgoMIV G'CCGG_C
Cuts at: 2558 2558
Size: 4379
NspI r_CATG'y
Cuts at: 1282 1432 1932 2839 1282
Size: 150 500 907 2822
Fragments arranged by size:
2822 907 500 150
PciI A'CATG_T
Cuts at: 2835 2835
Size: 4379
PflMI CCAn_nnn'nTGG
Cuts at: 882 882
Size: 4379
PfoI T'CCnGG_A
Cuts at: 404 695 404
Size: 291 4088
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 4347
PpuMI rG'GwC_Cy
Cuts at: 831 831
Size: 4379
PspOMI G'GGCC_C
Cuts at: 377 377
Size: 4379
PstI C_TGCA'G
Cuts at: 200 983 1426 2111 200
Size: 783 443 685 2468
Fragments arranged by size:
2468 783 685 443
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4379
PvuII CAG'CTG
Cuts at: 1804 2164 1804
Size: 360 4019
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4379
SacI G_AGCT'C
Cuts at: 1393 1393
Size: 4379
SalI G'TCGA_C
Cuts at: 1416 1416
Size: 4379
SapI GCTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4169
SbfI CC_TGCA'GG
Cuts at: 1426 1426
Size: 4379
ScaI AGT'ACT
Cuts at: 3896 3896
Size: 4379
SfcI C'TryA_G
Cuts at: 33 196 979 1422 2107 3100 3291 33
Size: 163 783 443 685 993 191 1121
Fragments arranged by size:
1121 993 783 685 443 191 163
SfoI GGC'GCC
Cuts at: 1811 2059 1811
Size: 248 4131
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
SmaI CCC'GGG
Cuts at: 950 1401 950
Size: 451 3928
SmlI C'TyrA_G
Cuts at: 1534 2449 2941 3203 1534
Size: 915 492 262 2710
Fragments arranged by size:
2710 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4379
SphI G_CATG'C
Cuts at: 1432 1932 1432
Size: 500 3879
StuI AGG'CCT
Cuts at: 1235 1296 1235
Size: 61 4318
StyI C'CwwG_G
Cuts at: 107 180 923 1045 1169 1513 1867 107
Size: 73 743 122 124 344 354 2619
Fragments arranged by size:
2619 743 354 344 124 122 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 276 1228 1342 1962 2095 2554 2763 276
Size: 952 114 620 133 459 209 1892
Fragments arranged by size:
1892 952 620 459 209 133 114
TatI w'GTAC_w
Cuts at: 3894 3894
Size: 4379
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4347
Tth111I GACn'n_nGTC
Cuts at: 2176 2176
Size: 4379
XbaI T'CTAG_A
Cuts at: 1410 1410
Size: 4379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 122 1874 122
Size: 1752 2627
XmaI C'CCGG_G
Cuts at: 948 1399 948
Size: 451 3928
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI