Restriction map for
pF1KE0253
(Circular) (Six-base) MAPSORT of: pF1KE0253.seq Check: 8676 from: 1 to: 4289
pF1KE0253 4289 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1327 1327
Size: 4289
Acc65I G'GTAC_C
Cuts at: 689 1305 689
Size: 616 3673
AclI AA'CG_TT
Cuts at: 4129 4129
Size: 4289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 613 2114 2546 3293 3561 306
Size: 307 1501 432 747 268 1034
Fragments arranged by size:
1501 1034 747 432 307 268
AflIII A'CryG_T
Cuts at: 1641 2745 3722 1641
Size: 1104 977 2208
Fragments arranged by size:
2208 1104 977
AgeI A'CCGG_T
Cuts at: 2677 2677
Size: 4289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1075 1075
Size: 4289
AlwNI CAG_nnn'CTG
Cuts at: 356 1594 3161 356
Size: 1238 1567 1484
Fragments arranged by size:
1567 1484 1238
ApaLI G'TGCA_C
Cuts at: 3059 3059
Size: 4289
ApoI r'AATT_y
Cuts at: 670 823 1293 3642 3898 670
Size: 153 470 2349 256 1061
Fragments arranged by size:
2349 1061 470 256 153
AseI AT'TA_AT
Cuts at: 20 20
Size: 4289
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
AvaI C'yCGr_G
Cuts at: 1309 1309
Size: 4289
BamHI G'GATC_C
Cuts at: 1314 1314
Size: 4289
BanI G'GyrC_C
Cuts at: 689 1305 1719 1967 2002 689
Size: 616 414 248 35 2976
Fragments arranged by size:
2976 616 414 248 35
BanII G_rGCy'C
Cuts at: 241 1127 1303 2333 241
Size: 886 176 1030 2197
Fragments arranged by size:
2197 1030 886 176
BbeI G_GCGC'C
Cuts at: 1723 1971 1723
Size: 248 4041
BbsI GAAGACnn'nnnn_
Cuts at: 801 801
Size: 4289
BbvCI CC'TCA_GC
Cuts at: 514 1048 514
Size: 534 3755
BciVI GTATCCnnnnn_n'
Cuts at: 1161 2187 2948 4289 1161
Size: 1026 761 1341 1161
Fragments arranged by size:
1341 1161 1026 761
BclI T'GATC_A
Cuts at: 881 1809 881
Size: 928 3361
BglII A'GATC_T
Cuts at: 1804 3841 1804
Size: 2037 2252
BlpI GC'TnA_GC
Cuts at: 1401 1401
Size: 4289
Bme1580I G_kGCm'C
Cuts at: 1914 2007 3063 1914
Size: 93 1056 3140
Fragments arranged by size:
3140 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 308 1276 1501 1582 1917 4061 308
Size: 968 225 81 335 2144 536
Fragments arranged by size:
2144 968 536 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 300 1146 2656 300
Size: 846 1510 1933
Fragments arranged by size:
1933 1510 846
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 915 661
Size: 254 4035
Bpu10I CC'TnA_GC
Cuts at: 514 1048 514
Size: 534 3755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1465 2344 2836 3134 1465
Size: 879 492 298 2620
Fragments arranged by size:
2620 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 296 856 3993 296
Size: 560 3137 592
Fragments arranged by size:
3137 592 560
BsaAI yAC'GTr
Cuts at: 345 1642 345
Size: 1297 2992
BsaBI GATnn'nnATC
Cuts at: 6 1803 3840 6
Size: 1797 2037 455
Fragments arranged by size:
2037 1797 455
BsaHI Gr'CG_yC
Cuts at: 1720 1968 2670 4166 1720
Size: 248 702 1496 1843
Fragments arranged by size:
1843 1496 702 248
BsaWI w'CCGG_w
Cuts at: 212 1478 1999 2677 2951 3098 3509 212
Size: 1266 521 678 274 147 411 992
Fragments arranged by size:
1266 992 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 273 1036 273
Size: 763 3526
BseYI C'CCAG_C
Cuts at: 359 1032 1714 2472 3049 3545 359
Size: 673 682 758 577 496 1103
Fragments arranged by size:
1103 758 682 673 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 601
Size: 4289
BsiEI CG_ry'CG
Cuts at: 104 1831 1877 3085 104
Size: 1727 46 1208 1308
Fragments arranged by size:
1727 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1127 1303 2081 2271 3063 1127
Size: 176 778 190 792 2353
Fragments arranged by size:
2353 792 778 190 176
BsmI GAATG_Cn'
Cuts at: 265 265
Size: 4289
Bsp1286I G_dGCh'C
Cuts at: 241 1127 1303 1914 2007 2081 2271 2333 3063
Size: 886 176 611 93 74 190 62 730
Cuts at: 3063 241
Size: 1467
Fragments arranged by size:
1467 886 730 611 190 176 93 74
62
BspEI T'CCGG_A
Cuts at: 3509 3509
Size: 4289
BspHI T'CATG_A
Cuts at: 3465 3465
Size: 4289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 482 1339 1553 1855 2236 482
Size: 857 214 302 381 2535
Fragments arranged by size:
2535 857 381 302 214
BsrBI CCG'CTC
Cuts at: 50 687 2581 2635 4119 4284 50
Size: 637 1894 54 1484 165 55
Fragments arranged by size:
1894 1484 637 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2201 2201
Size: 4289
BsrFI r'CCGG_y
Cuts at: 477 2287 2468 2677 477
Size: 1810 181 209 2089
Fragments arranged by size:
2089 1810 209 181
BssSI C'ACGA_G
Cuts at: 577 2560 2918 577
Size: 1983 358 1948
Fragments arranged by size:
1983 1948 358
BstAPI GCAn_nnn'nTGC
Cuts at: 588 1666 588
Size: 1078 3211
BstBI TT'CG_AA
Cuts at: 2650 2650
Size: 4289
BstYI r'GATC_y
Cuts at: 1 596 1314 1781 1804 2139 2385 3397 3483
Size: 595 718 467 23 335 246 1012 86
Cuts at: 3483 3495 3841 1
Size: 12 346 449
Fragments arranged by size:
1012 718 595 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 739 739
Size: 4289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2239 2420 2583 2239
Size: 181 163 3945
Fragments arranged by size:
3945 181 163
BtsI GCAGTG_nn'
Cuts at: 367 1532 367
Size: 1165 3124
DraI TTT'AAA
Cuts at: 1288 1288
Size: 4289
DrdI GACnn_nn'nnGTC
Cuts at: 217 903 1995 2853 217
Size: 686 1092 858 1653
Fragments arranged by size:
1653 1092 858 686
EaeI y'GGCC_r
Cuts at: 396 1874 2048 2439 2466 3811 396
Size: 1478 174 391 27 1345 874
Fragments arranged by size:
1478 1345 874 391 174 27
EagI C'GGCC_G
Cuts at: 1874 1874
Size: 4289
EarI CTCTTCn'nnn_
Cuts at: 601 2312 2522 601
Size: 1711 210 2368
Fragments arranged by size:
2368 1711 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 562 759 2497 2807 2953 4099 562
Size: 197 1738 310 146 1146 752
Fragments arranged by size:
1738 1146 752 310 197 146
EcoICRI GAG'CTC
Cuts at: 1125 1301 1125
Size: 176 4113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 613 661 915 2114 2546 3293 3561 306
Size: 307 48 254 1199 432 747 268 1034
Fragments arranged by size:
1199 1034 747 432 307 268 254 48
EcoO109I rG'GnC_Cy
Cuts at: 172 432 1428 172
Size: 260 996 3033
Fragments arranged by size:
3033 996 260
EcoRI G'AATT_C
Cuts at: 670 823 1293 670
Size: 153 470 3666
Fragments arranged by size:
3666 470 153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 4289
FspI TGC'GCA
Cuts at: 2070 2070
Size: 4289
HaeII r_GCGC'y
Cuts at: 1723 1971 2993 3972 1723
Size: 248 1022 979 2040
Fragments arranged by size:
2040 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 164 196 1058 1090 1100 1132 2131 2161 2163
Size: 32 862 32 10 32 999 30 2
Cuts at: 2163 2193 164
Size: 30 2260
Fragments arranged by size:
2260 999 862 32 32 32 30 30
10 2
HincII GTy'rAC
Cuts at: 1328 1328
Size: 4289
KasI G'GCGC_C
Cuts at: 1719 1967 1719
Size: 248 4041
KpnI G_GTAC'C
Cuts at: 693 1309 693
Size: 616 3673
MluI A'CGCG_T
Cuts at: 3722 3722
Size: 4289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 638 2960 3144 638
Size: 2322 184 1783
Fragments arranged by size:
2322 1783 184
MscI TGG'CCA
Cuts at: 398 2050 398
Size: 1652 2637
MslI CAynn'nnrTG
Cuts at: 803 987 2278 2405 803
Size: 184 1291 127 2687
Fragments arranged by size:
2687 1291 184 127
MspA1I CmG'CkG
Cuts at: 127 302 502 830 1400 1714 2074 3087 3332
Size: 175 200 328 570 314 360 1013 245
Cuts at: 3332 127
Size: 1084
Fragments arranged by size:
1084 1013 570 360 328 314 245 200
175
NaeI GCC'GGC
Cuts at: 2470 2470
Size: 4289
NarI GG'CG_CC
Cuts at: 1720 1968 1720
Size: 248 4041
NgoMIV G'CCGG_C
Cuts at: 2468 2468
Size: 4289
NspI r_CATG'y
Cuts at: 141 591 701 890 1271 1342 1842 2749 141
Size: 450 110 189 381 71 500 907 1681
Fragments arranged by size:
1681 907 500 450 381 189 110 71
PciI A'CATG_T
Cuts at: 2745 2745
Size: 4289
PfoI T'CCnGG_A
Cuts at: 815 815
Size: 4289
PmeI GTTT'AAAC
Cuts at: 1288 1288
Size: 4289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 4257
PpuMI rG'GwC_Cy
Cuts at: 172 172
Size: 4289
PshAI GACnn'nnGTC
Cuts at: 868 868
Size: 4289
PsiI TTA'TAA
Cuts at: 560 560
Size: 4289
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 693 725 693
Size: 32 4257
PstI C_TGCA'G
Cuts at: 132 1336 2021 132
Size: 1204 685 2400
Fragments arranged by size:
2400 1204 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4289
PvuII CAG'CTG
Cuts at: 127 302 502 830 1714 2074 127
Size: 175 200 328 884 360 2342
Fragments arranged by size:
2342 884 360 328 200 175
RsrII CG'GwC_CG
Cuts at: 2484 2484
Size: 4289
SacI G_AGCT'C
Cuts at: 1127 1303 1127
Size: 176 4113
SalI G'TCGA_C
Cuts at: 1326 1326
Size: 4289
SanDI GG'GwC_CC
Cuts at: 172 172
Size: 4289
SapI GCTCTTCn'nnn_
Cuts at: 2312 2522 2312
Size: 210 4079
SbfI CC_TGCA'GG
Cuts at: 1336 1336
Size: 4289
ScaI AGT'ACT
Cuts at: 3806 3806
Size: 4289
SfcI C'TryA_G
Cuts at: 33 128 1332 2017 3010 3201 33
Size: 95 1204 685 993 191 1121
Fragments arranged by size:
1204 1121 993 685 191 95
SfoI GGC'GCC
Cuts at: 1721 1969 1721
Size: 248 4041
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
SmaI CCC'GGG
Cuts at: 1311 1311
Size: 4289
SmlI C'TyrA_G
Cuts at: 1444 2359 2851 3113 1444
Size: 915 492 262 2620
Fragments arranged by size:
2620 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4289
SphI G_CATG'C
Cuts at: 1342 1842 1342
Size: 500 3789
SspI AAT'ATT
Cuts at: 1191 1191
Size: 4289
StyI C'CwwG_G
Cuts at: 786 1423 1777 786
Size: 637 354 3298
Fragments arranged by size:
3298 637 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1192 1295 1872 2005 2464 2673 1192
Size: 103 577 133 459 209 2808
Fragments arranged by size:
2808 577 459 209 133 103
TatI w'GTAC_w
Cuts at: 134 3804 134
Size: 3670 619
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3806 3838 3806
Size: 32 4257
Tth111I GACn'n_nGTC
Cuts at: 191 2086 191
Size: 1895 2394
XbaI T'CTAG_A
Cuts at: 1320 1320
Size: 4289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1784 1784
Size: 4289
XmaI C'CCGG_G
Cuts at: 1309 1309
Size: 4289
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PspOMI PspXI SacII
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI