Restriction map for
pF1KE0251
(Circular) (Six-base) MAPSORT of: pF1KE0251.seq Check: 3010 from: 1 to: 3380
pF1KE0251 3380 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 418 418
Size: 3380
Acc65I G'GTAC_C
Cuts at: 396 396
Size: 3380
AclI AA'CG_TT
Cuts at: 3220 3220
Size: 3380
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1205 1637 2384 2652 1205
Size: 432 747 268 1933
Fragments arranged by size:
1933 747 432 268
AflIII A'CryG_T
Cuts at: 732 1836 2813 732
Size: 1104 977 1299
Fragments arranged by size:
1299 1104 977
AgeI A'CCGG_T
Cuts at: 1768 1768
Size: 3380
AlwNI CAG_nnn'CTG
Cuts at: 180 248 685 2252 180
Size: 68 437 1567 1308
Fragments arranged by size:
1567 1308 437 68
ApaI G_GGCC'C
Cuts at: 230 230
Size: 3380
ApaLI G'TGCA_C
Cuts at: 2150 2150
Size: 3380
ApoI r'AATT_y
Cuts at: 384 2733 2989 384
Size: 2349 256 775
Fragments arranged by size:
2349 775 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3380
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3380
AvaI C'yCGr_G
Cuts at: 400 400
Size: 3380
BamHI G'GATC_C
Cuts at: 405 405
Size: 3380
BanI G'GyrC_C
Cuts at: 396 810 1058 1093 396
Size: 414 248 35 2683
Fragments arranged by size:
2683 414 248 35
BanII G_rGCy'C
Cuts at: 169 230 301 343 394 1424 169
Size: 61 71 42 51 1030 2125
Fragments arranged by size:
2125 1030 71 61 51 42
BbeI G_GCGC'C
Cuts at: 814 1062 814
Size: 248 3132
BciVI GTATCCnnnnn_n'
Cuts at: 1278 2039 3380 1278
Size: 761 1341 1278
Fragments arranged by size:
1341 1278 761
BclI T'GATC_A
Cuts at: 900 900
Size: 3380
BglII A'GATC_T
Cuts at: 895 2932 895
Size: 2037 1343
BlpI GC'TnA_GC
Cuts at: 492 492
Size: 3380
Bme1580I G_kGCm'C
Cuts at: 157 207 230 1005 1098 2154 157
Size: 50 23 775 93 1056 1383
Fragments arranged by size:
1383 1056 775 93 50 23
BmrI ACTGGGnnnn_n'
Cuts at: 592 673 1008 3152 592
Size: 81 335 2144 820
Fragments arranged by size:
2144 820 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1747 1747
Size: 3380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 180 327 170
Size: 10 147 3223
Fragments arranged by size:
3223 147 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1435 1927 2225 556
Size: 879 492 298 1711
Fragments arranged by size:
1711 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 281 3084 281
Size: 2803 577
BsaAI yAC'GTr
Cuts at: 733 733
Size: 3380
BsaBI GATnn'nnATC
Cuts at: 6 894 2931 6
Size: 888 2037 455
Fragments arranged by size:
2037 888 455
BsaHI Gr'CG_yC
Cuts at: 811 1059 1761 3257 811
Size: 248 702 1496 934
Fragments arranged by size:
1496 934 702 248
BsaWI w'CCGG_w
Cuts at: 569 1090 1768 2042 2189 2600 569
Size: 521 678 274 147 411 1349
Fragments arranged by size:
1349 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 168 229 805 1563 2140 2636 168
Size: 61 576 758 577 496 912
Fragments arranged by size:
912 758 577 576 496 61
BsiEI CG_ry'CG
Cuts at: 104 922 968 2176 104
Size: 818 46 1208 1308
Fragments arranged by size:
1308 1208 818 46
BsiHKAI G_wGCw'C
Cuts at: 301 343 394 1172 1362 2154 301
Size: 42 51 778 190 792 1527
Fragments arranged by size:
1527 792 778 190 51 42
Bsp1286I G_dGCh'C
Cuts at: 157 169 207 230 301 343 394 1005 1098
Size: 12 38 23 71 42 51 611 93
Cuts at: 1098 1172 1362 1424 2154 157
Size: 74 190 62 730 1383
Fragments arranged by size:
1383 730 611 190 93 74 71 62
51 42 38 23 12
BspEI T'CCGG_A
Cuts at: 2600 2600
Size: 3380
BspHI T'CATG_A
Cuts at: 2556 2556
Size: 3380
BspMI ACCTGCnnnn'nnnn_
Cuts at: 263 430 644 946 1327 263
Size: 167 214 302 381 2316
Fragments arranged by size:
2316 381 302 214 167
BsrBI CCG'CTC
Cuts at: 50 1672 1726 3210 3375 50
Size: 1622 54 1484 165 55
Fragments arranged by size:
1622 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1292 1292
Size: 3380
BsrFI r'CCGG_y
Cuts at: 1378 1559 1768 1378
Size: 181 209 2990
Fragments arranged by size:
2990 209 181
BssSI C'ACGA_G
Cuts at: 1651 2009 1651
Size: 358 3022
BstAPI GCAn_nnn'nTGC
Cuts at: 248 757 248
Size: 509 2871
BstBI TT'CG_AA
Cuts at: 1741 1741
Size: 3380
BstYI r'GATC_y
Cuts at: 1 405 872 895 1230 1476 2488 2574 2586
Size: 404 467 23 335 246 1012 86 12
Cuts at: 2586 2932 1
Size: 346 449
Fragments arranged by size:
1012 467 449 404 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1330 1511 1674 1330
Size: 181 163 3036
Fragments arranged by size:
3036 181 163
BtsI GCAGTG_nn'
Cuts at: 139 623 139
Size: 484 2896
DraI TTT'AAA
Cuts at: 379 379
Size: 3380
DrdI GACnn_nn'nnGTC
Cuts at: 1086 1944 1086
Size: 858 2522
EaeI y'GGCC_r
Cuts at: 351 965 1139 1530 1557 2902 351
Size: 614 174 391 27 1345 829
Fragments arranged by size:
1345 829 614 391 174 27
EagI C'GGCC_G
Cuts at: 965 965
Size: 3380
EarI CTCTTCn'nnn_
Cuts at: 1403 1613 1403
Size: 210 3170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 134 1588 1898 2044 3190 134
Size: 1454 310 146 1146 324
Fragments arranged by size:
1454 1146 324 310 146
EcoICRI GAG'CTC
Cuts at: 299 341 392 299
Size: 42 51 3287
Fragments arranged by size:
3287 51 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 180 327 1205 1637 2384 2652 170
Size: 10 147 878 432 747 268 898
Fragments arranged by size:
898 878 747 432 268 147 10
EcoNI CCTnn'n_nnAGG
Cuts at: 312 312
Size: 3380
EcoO109I rG'GnC_Cy
Cuts at: 519 519
Size: 3380
EcoRI G'AATT_C
Cuts at: 384 384
Size: 3380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1399 1399
Size: 3380
FspI TGC'GCA
Cuts at: 1161 1161
Size: 3380
HaeII r_GCGC'y
Cuts at: 814 1062 2084 3063 814
Size: 248 1022 979 1131
Fragments arranged by size:
1131 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1222 1252 1254 1284 1222
Size: 30 2 30 3318
Fragments arranged by size:
3318 30 30 2
HincII GTy'rAC
Cuts at: 419 419
Size: 3380
KasI G'GCGC_C
Cuts at: 810 1058 810
Size: 248 3132
KpnI G_GTAC'C
Cuts at: 400 400
Size: 3380
MluI A'CGCG_T
Cuts at: 2813 2813
Size: 3380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 326 2051 2235 326
Size: 1725 184 1471
Fragments arranged by size:
1725 1471 184
MscI TGG'CCA
Cuts at: 353 1141 353
Size: 788 2592
MslI CAynn'nnrTG
Cuts at: 1369 1496 1369
Size: 127 3253
MspA1I CmG'CkG
Cuts at: 200 245 347 491 805 1165 2178 2423 200
Size: 45 102 144 314 360 1013 245 1157
Fragments arranged by size:
1157 1013 360 314 245 144 102 45
NaeI GCC'GGC
Cuts at: 1561 1561
Size: 3380
NarI GG'CG_CC
Cuts at: 811 1059 811
Size: 248 3132
NgoMIV G'CCGG_C
Cuts at: 1559 1559
Size: 3380
NspI r_CATG'y
Cuts at: 433 933 1840 433
Size: 500 907 1973
Fragments arranged by size:
1973 907 500
PciI A'CATG_T
Cuts at: 1836 1836
Size: 3380
PflMI CCAn_nnn'nTGG
Cuts at: 214 214
Size: 3380
PmeI GTTT'AAAC
Cuts at: 379 379
Size: 3380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2519 2551 2519
Size: 32 3348
PspOMI G'GGCC_C
Cuts at: 226 226
Size: 3380
PstI C_TGCA'G
Cuts at: 427 1112 427
Size: 685 2695
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3380
PvuII CAG'CTG
Cuts at: 200 245 347 805 1165 200
Size: 45 102 458 360 2415
Fragments arranged by size:
2415 458 360 102 45
RsrII CG'GwC_CG
Cuts at: 1575 1575
Size: 3380
SacI G_AGCT'C
Cuts at: 301 343 394 301
Size: 42 51 3287
Fragments arranged by size:
3287 51 42
SalI G'TCGA_C
Cuts at: 417 417
Size: 3380
SapI GCTCTTCn'nnn_
Cuts at: 1403 1613 1403
Size: 210 3170
SbfI CC_TGCA'GG
Cuts at: 427 427
Size: 3380
ScaI AGT'ACT
Cuts at: 2897 2897
Size: 3380
SfcI C'TryA_G
Cuts at: 33 423 1108 2101 2292 33
Size: 390 685 993 191 1121
Fragments arranged by size:
1121 993 685 390 191
SfoI GGC'GCC
Cuts at: 812 1060 812
Size: 248 3132
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3380
SmaI CCC'GGG
Cuts at: 402 402
Size: 3380
SmlI C'TyrA_G
Cuts at: 535 1450 1942 2204 535
Size: 915 492 262 1711
Fragments arranged by size:
1711 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3380
SphI G_CATG'C
Cuts at: 433 933 433
Size: 500 2880
StyI C'CwwG_G
Cuts at: 514 868 514
Size: 354 3026
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 963 1096 1555 1764 963
Size: 133 459 209 2579
Fragments arranged by size:
2579 459 209 133
TatI w'GTAC_w
Cuts at: 2895 2895
Size: 3380
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 3348
Tth111I GACn'n_nGTC
Cuts at: 1177 1177
Size: 3380
XbaI T'CTAG_A
Cuts at: 411 411
Size: 3380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 875 875
Size: 3380
XmaI C'CCGG_G
Cuts at: 400 400
Size: 3380
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI