Restriction map for
pF1KE0249
(Circular) (Six-base) MAPSORT of: pF1KE0249.seq Check: 8970 from: 1 to: 3365
pF1KE0249 3365 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 403 403
Size: 3365
Acc65I G'GTAC_C
Cuts at: 381 381
Size: 3365
AclI AA'CG_TT
Cuts at: 3205 3205
Size: 3365
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1622 2369 2637 1190
Size: 432 747 268 1918
Fragments arranged by size:
1918 747 432 268
AflIII A'CryG_T
Cuts at: 717 1821 2798 717
Size: 1104 977 1284
Fragments arranged by size:
1284 1104 977
AgeI A'CCGG_T
Cuts at: 1753 1753
Size: 3365
AlwNI CAG_nnn'CTG
Cuts at: 670 2237 670
Size: 1567 1798
ApaLI G'TGCA_C
Cuts at: 2135 2135
Size: 3365
ApoI r'AATT_y
Cuts at: 369 2718 2974 369
Size: 2349 256 760
Fragments arranged by size:
2349 760 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3365
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3365
AvaI C'yCGr_G
Cuts at: 385 385
Size: 3365
BamHI G'GATC_C
Cuts at: 390 390
Size: 3365
BanI G'GyrC_C
Cuts at: 123 264 381 795 1043 1078 123
Size: 141 117 414 248 35 2410
Fragments arranged by size:
2410 414 248 141 117 35
BanII G_rGCy'C
Cuts at: 379 1409 379
Size: 1030 2335
BbeI G_GCGC'C
Cuts at: 127 799 1047 127
Size: 672 248 2445
Fragments arranged by size:
2445 672 248
BbvCI CC'TCA_GC
Cuts at: 239 239
Size: 3365
BciVI GTATCCnnnnn_n'
Cuts at: 1263 2024 3365 1263
Size: 761 1341 1263
Fragments arranged by size:
1341 1263 761
BclI T'GATC_A
Cuts at: 885 885
Size: 3365
BglII A'GATC_T
Cuts at: 880 2917 880
Size: 2037 1328
BlpI GC'TnA_GC
Cuts at: 477 477
Size: 3365
Bme1580I G_kGCm'C
Cuts at: 990 1083 2139 990
Size: 93 1056 2216
Fragments arranged by size:
2216 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 577 658 993 3137 577
Size: 81 335 2144 805
Fragments arranged by size:
2144 805 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1732 1732
Size: 3365
Bpu10I CC'TnA_GC
Cuts at: 239 239
Size: 3365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 1420 1912 2210 541
Size: 879 492 298 1696
Fragments arranged by size:
1696 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3069 3069
Size: 3365
BsaAI yAC'GTr
Cuts at: 718 718
Size: 3365
BsaBI GATnn'nnATC
Cuts at: 6 879 2916 6
Size: 873 2037 455
Fragments arranged by size:
2037 873 455
BsaHI Gr'CG_yC
Cuts at: 124 796 1044 1746 3242 124
Size: 672 248 702 1496 247
Fragments arranged by size:
1496 702 672 248 247
BsaWI w'CCGG_w
Cuts at: 554 1075 1753 2027 2174 2585 554
Size: 521 678 274 147 411 1334
Fragments arranged by size:
1334 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 278 790 1548 2125 2621 278
Size: 512 758 577 496 1022
Fragments arranged by size:
1022 758 577 512 496
BsiEI CG_ry'CG
Cuts at: 104 907 953 2161 104
Size: 803 46 1208 1308
Fragments arranged by size:
1308 1208 803 46
BsiHKAI G_wGCw'C
Cuts at: 379 1157 1347 2139 379
Size: 778 190 792 1605
Fragments arranged by size:
1605 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 379 990 1083 1157 1347 1409 2139 379
Size: 611 93 74 190 62 730 1605
Fragments arranged by size:
1605 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2585 2585
Size: 3365
BspHI T'CATG_A
Cuts at: 2541 2541
Size: 3365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 415 629 931 1312 415
Size: 214 302 381 2468
Fragments arranged by size:
2468 381 302 214
BsrBI CCG'CTC
Cuts at: 50 214 1657 1711 3195 3360 50
Size: 164 1443 54 1484 165 55
Fragments arranged by size:
1484 1443 165 164 55 54
BsrDI GCAATG_nn'
Cuts at: 1277 1277
Size: 3365
BsrFI r'CCGG_y
Cuts at: 1363 1544 1753 1363
Size: 181 209 2975
Fragments arranged by size:
2975 209 181
BssSI C'ACGA_G
Cuts at: 1636 1994 1636
Size: 358 3007
BstAPI GCAn_nnn'nTGC
Cuts at: 742 742
Size: 3365
BstBI TT'CG_AA
Cuts at: 1726 1726
Size: 3365
BstYI r'GATC_y
Cuts at: 1 390 857 880 1215 1461 2473 2559 2571
Size: 389 467 23 335 246 1012 86 12
Cuts at: 2571 2917 1
Size: 346 449
Fragments arranged by size:
1012 467 449 389 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3365
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1315 1496 1659 1315
Size: 181 163 3021
Fragments arranged by size:
3021 181 163
BtsI GCAGTG_nn'
Cuts at: 608 608
Size: 3365
DraI TTT'AAA
Cuts at: 364 364
Size: 3365
DrdI GACnn_nn'nnGTC
Cuts at: 1071 1929 1071
Size: 858 2507
EaeI y'GGCC_r
Cuts at: 229 950 1124 1515 1542 2887 229
Size: 721 174 391 27 1345 707
Fragments arranged by size:
1345 721 707 391 174 27
EagI C'GGCC_G
Cuts at: 950 950
Size: 3365
EarI CTCTTCn'nnn_
Cuts at: 173 1388 1598 173
Size: 1215 210 1940
Fragments arranged by size:
1940 1215 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1573 1883 2029 3175 1573
Size: 310 146 1146 1763
Fragments arranged by size:
1763 1146 310 146
EcoICRI GAG'CTC
Cuts at: 377 377
Size: 3365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1622 2369 2637 1190
Size: 432 747 268 1918
Fragments arranged by size:
1918 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 141 504 141
Size: 363 3002
EcoRI G'AATT_C
Cuts at: 369 369
Size: 3365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1384 1384
Size: 3365
FspI TGC'GCA
Cuts at: 1146 1146
Size: 3365
HaeII r_GCGC'y
Cuts at: 127 206 799 1047 2069 3048 127
Size: 79 593 248 1022 979 444
Fragments arranged by size:
1022 979 593 444 248 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1207 1237 1239 1269 1207
Size: 30 2 30 3303
Fragments arranged by size:
3303 30 30 2
HincII GTy'rAC
Cuts at: 404 404
Size: 3365
KasI G'GCGC_C
Cuts at: 123 795 1043 123
Size: 672 248 2445
Fragments arranged by size:
2445 672 248
KpnI G_GTAC'C
Cuts at: 385 385
Size: 3365
MluI A'CGCG_T
Cuts at: 2798 2798
Size: 3365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2036 2220 2036
Size: 184 3181
MscI TGG'CCA
Cuts at: 1126 1126
Size: 3365
MslI CAynn'nnrTG
Cuts at: 1354 1481 1354
Size: 127 3238
MspA1I CmG'CkG
Cuts at: 227 273 282 476 790 1150 2163 2408 227
Size: 46 9 194 314 360 1013 245 1184
Fragments arranged by size:
1184 1013 360 314 245 194 46 9
NaeI GCC'GGC
Cuts at: 1546 1546
Size: 3365
NarI GG'CG_CC
Cuts at: 124 796 1044 124
Size: 672 248 2445
Fragments arranged by size:
2445 672 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3365
NgoMIV G'CCGG_C
Cuts at: 1544 1544
Size: 3365
NspI r_CATG'y
Cuts at: 418 918 1825 418
Size: 500 907 1958
Fragments arranged by size:
1958 907 500
PasI CC'CwG_GG
Cuts at: 297 297
Size: 3365
PciI A'CATG_T
Cuts at: 1821 1821
Size: 3365
PmeI GTTT'AAAC
Cuts at: 364 364
Size: 3365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2504 2536 2504
Size: 32 3333
PpuMI rG'GwC_Cy
Cuts at: 141 141
Size: 3365
PstI C_TGCA'G
Cuts at: 412 1097 412
Size: 685 2680
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3365
PvuII CAG'CTG
Cuts at: 790 1150 790
Size: 360 3005
RsrII CG'GwC_CG
Cuts at: 1560 1560
Size: 3365
SacI G_AGCT'C
Cuts at: 379 379
Size: 3365
SalI G'TCGA_C
Cuts at: 402 402
Size: 3365
SanDI GG'GwC_CC
Cuts at: 141 141
Size: 3365
SapI GCTCTTCn'nnn_
Cuts at: 173 1388 1598 173
Size: 1215 210 1940
Fragments arranged by size:
1940 1215 210
SbfI CC_TGCA'GG
Cuts at: 412 412
Size: 3365
ScaI AGT'ACT
Cuts at: 2882 2882
Size: 3365
SfcI C'TryA_G
Cuts at: 33 408 1093 2086 2277 33
Size: 375 685 993 191 1121
Fragments arranged by size:
1121 993 685 375 191
SfoI GGC'GCC
Cuts at: 125 797 1045 125
Size: 672 248 2445
Fragments arranged by size:
2445 672 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3365
SmaI CCC'GGG
Cuts at: 387 387
Size: 3365
SmlI C'TyrA_G
Cuts at: 520 1435 1927 2189 520
Size: 915 492 262 1696
Fragments arranged by size:
1696 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3365
SphI G_CATG'C
Cuts at: 418 918 418
Size: 500 2865
StyI C'CwwG_G
Cuts at: 107 499 853 107
Size: 392 354 2619
Fragments arranged by size:
2619 392 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 310 948 1081 1540 1749 310
Size: 638 133 459 209 1926
Fragments arranged by size:
1926 638 459 209 133
TatI w'GTAC_w
Cuts at: 2880 2880
Size: 3365
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2882 2914 2882
Size: 32 3333
Tth111I GACn'n_nGTC
Cuts at: 1162 1162
Size: 3365
XbaI T'CTAG_A
Cuts at: 396 396
Size: 3365
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 860 860
Size: 3365
XmaI C'CCGG_G
Cuts at: 385 385
Size: 3365
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI