Restriction map for
pF1KE0246
(Circular) (Six-base) MAPSORT of: pF1KE0246.seq Check: 3449 from: 1 to: 4367
pF1KE0246 4367 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1405 1405
Size: 4367
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2192 2624 3371 3639 2192
Size: 432 747 268 2920
Fragments arranged by size:
2920 747 432 268
AfeI AGC'GCT
Cuts at: 604 604
Size: 4367
AflIII A'CryG_T
Cuts at: 230 1719 2823 3800 230
Size: 1489 1104 977 797
Fragments arranged by size:
1489 1104 977 797
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AhdI GACnn_n'nnGTC
Cuts at: 1311 1311
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 396 999 1672 3239 396
Size: 603 673 1567 1524
Fragments arranged by size:
1567 1524 673 603
ApaLI G'TGCA_C
Cuts at: 496 777 936 3137 496
Size: 281 159 2201 1726
Fragments arranged by size:
2201 1726 281 159
ApoI r'AATT_y
Cuts at: 1371 3720 3976 1371
Size: 2349 256 1762
Fragments arranged by size:
2349 1762 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 138 204 1293 1387 138
Size: 66 1089 94 3118
Fragments arranged by size:
3118 1089 94 66
BamHI G'GATC_C
Cuts at: 1392 1392
Size: 4367
BanI G'GyrC_C
Cuts at: 252 708 1073 1383 1797 2045 2080 252
Size: 456 365 310 414 248 35 2539
Fragments arranged by size:
2539 456 414 365 310 248 35
BanII G_rGCy'C
Cuts at: 1381 2411 1381
Size: 1030 3337
BbeI G_GCGC'C
Cuts at: 1077 1801 2049 1077
Size: 724 248 3395
Fragments arranged by size:
3395 724 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 957 991 90
Size: 34 833 34 3466
Fragments arranged by size:
3466 833 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BglI GCCn_nnn'nGGC
Cuts at: 703 1243 703
Size: 540 3827
BglII A'GATC_T
Cuts at: 1882 3919 1882
Size: 2037 2330
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 145 340 500 781 940 1992 2085 3141 145
Size: 195 160 281 159 1052 93 1056 1371
Fragments arranged by size:
1371 1056 1052 281 195 160 159 93
BmgBI CAC'GTC
Cuts at: 273 273
Size: 4367
BmrI ACTGGGnnnn_n'
Cuts at: 1579 1660 1995 4139 1579
Size: 81 335 2144 1807
Fragments arranged by size:
2144 1807 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 1317 205
Size: 1112 3255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1543 2422 2914 3212 1543
Size: 879 492 298 2698
Fragments arranged by size:
2698 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4071 4071
Size: 4367
BsaAI yAC'GTr
Cuts at: 1024 1068 1720 1024
Size: 44 652 3671
Fragments arranged by size:
3671 652 44
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 521 554 1074 1798 2046 2748 4244 521
Size: 33 520 724 248 702 1496 644
Fragments arranged by size:
1496 724 702 644 520 248 33
BsaWI w'CCGG_w
Cuts at: 1556 2077 2755 3029 3176 3587 1556
Size: 521 678 274 147 411 2336
Fragments arranged by size:
2336 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 274 304 1159 1189 1315 1345 274
Size: 30 855 30 126 30 3296
Fragments arranged by size:
3296 855 126 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 202
Size: 4367
BseYI C'CCAG_C
Cuts at: 256 1792 2550 3127 3623 256
Size: 1536 758 577 496 1000
Fragments arranged by size:
1536 1000 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 543 1046 543
Size: 503 3864
BsiEI CG_ry'CG
Cuts at: 104 152 920 944 1909 1955 3163 104
Size: 48 768 24 965 46 1208 1308
Fragments arranged by size:
1308 1208 965 768 48 46 24
BsiHKAI G_wGCw'C
Cuts at: 406 500 781 940 956 1381 2159 2349 3141
Size: 94 281 159 16 425 778 190 792
Cuts at: 3141 406
Size: 1632
Fragments arranged by size:
1632 792 778 425 281 190 159 94
16
BsmBI CGTCTCn'nnnn_
Cuts at: 1212 1345 1212
Size: 133 4234
Bsp1286I G_dGCh'C
Cuts at: 145 340 406 500 781 940 956 1381 1992
Size: 195 66 94 281 159 16 425 611
Cuts at: 1992 2085 2159 2349 2411 3141 145
Size: 93 74 190 62 730 1371
Fragments arranged by size:
1371 730 611 425 281 195 190 159
94 93 74 66 62 16
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 3543 3543
Size: 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 452 1417 1631 1933 2314 452
Size: 965 214 302 381 2505
Fragments arranged by size:
2505 965 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2659 2713 4197 4362 50
Size: 2609 54 1484 165 55
Fragments arranged by size:
2609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1344 2279 1344
Size: 935 3432
BsrFI r'CCGG_y
Cuts at: 147 523 763 2365 2546 2755 147
Size: 376 240 1602 181 209 1759
Fragments arranged by size:
1759 1602 376 240 209 181
BsrGI T'GTAC_A
Cuts at: 479 1196 479
Size: 717 3650
BssSI C'ACGA_G
Cuts at: 2638 2996 2638
Size: 358 4009
BstAPI GCAn_nnn'nTGC
Cuts at: 396 1011 1744 396
Size: 615 733 3019
Fragments arranged by size:
3019 733 615
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstEII G'GTnAC_C
Cuts at: 303 1149 303
Size: 846 3521
BstXI CCAn_nnnn'nTGG
Cuts at: 300 471 300
Size: 171 4196
BstYI r'GATC_y
Cuts at: 1 1392 1859 1882 2217 2463 3475 3561 3573
Size: 1391 467 23 335 246 1012 86 12
Cuts at: 3573 3919 1
Size: 346 449
Fragments arranged by size:
1391 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 470 531 569 725 851 470
Size: 61 38 156 126 3986
Fragments arranged by size:
3986 156 126 61 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 867 1020 2317 2498 2661 867
Size: 153 1297 181 163 2573
Fragments arranged by size:
2573 1297 181 163 153
BtsI GCAGTG_nn'
Cuts at: 794 1034 1111 1610 794
Size: 240 77 499 3551
Fragments arranged by size:
3551 499 240 77
DraI TTT'AAA
Cuts at: 1366 1366
Size: 4367
DraIII CAC_nnn'GTG
Cuts at: 1011 1011
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 645 2073 2931 645
Size: 1428 858 2081
Fragments arranged by size:
2081 1428 858
EaeI y'GGCC_r
Cuts at: 149 461 584 611 753 917 971 1235 1952
Size: 312 123 27 142 164 54 264 717
Cuts at: 1952 2126 2517 2544 3889 149
Size: 174 391 27 1345 627
Fragments arranged by size:
1345 717 627 391 312 264 174 164
142 123 54 27 27
EagI C'GGCC_G
Cuts at: 149 917 1952 149
Size: 768 1035 2564
Fragments arranged by size:
2564 1035 768
EarI CTCTTCn'nnn_
Cuts at: 331 1168 1273 2390 2600 331
Size: 837 105 1117 210 2098
Fragments arranged by size:
2098 1117 837 210 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1210 2575 2885 3031 4177 1210
Size: 1365 310 146 1146 1400
Fragments arranged by size:
1400 1365 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 1317 2192 2624 3371 3639 205
Size: 1112 875 432 747 268 933
Fragments arranged by size:
1112 933 875 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1293 1293
Size: 4367
EcoO109I rG'GnC_Cy
Cuts at: 996 1506 996
Size: 510 3857
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4367
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 196 325 436 606 680 749 1077 1801 2049
Size: 129 111 170 74 69 328 724 248
Cuts at: 2049 3071 4050 196
Size: 1022 979 513
Fragments arranged by size:
1022 979 724 513 328 248 170 129
111 74 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 1297 1329 2209 2239 2241 2271 274
Size: 32 991 32 880 30 2 30 2370
Fragments arranged by size:
2370 991 880 32 32 30 30 2
HincII GTy'rAC
Cuts at: 825 1406 825
Size: 581 3786
KasI G'GCGC_C
Cuts at: 1073 1797 2045 1073
Size: 724 248 3395
Fragments arranged by size:
3395 724 248
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MluI A'CGCG_T
Cuts at: 230 3800 230
Size: 3570 797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 215 464 3038 3222 215
Size: 249 2574 184 1360
Fragments arranged by size:
2574 1360 249 184
MscI TGG'CCA
Cuts at: 463 586 613 973 1237 2128 463
Size: 123 27 360 264 891 2702
Fragments arranged by size:
2702 891 360 264 123 27
MslI CAynn'nnrTG
Cuts at: 469 493 832 2356 2483 469
Size: 24 339 1524 127 2353
Fragments arranged by size:
2353 1524 339 127 24
MspA1I CmG'CkG
Cuts at: 168 533 1478 1792 2152 3165 3410 168
Size: 365 945 314 360 1013 245 1125
Fragments arranged by size:
1125 1013 945 365 360 314 245
NaeI GCC'GGC
Cuts at: 149 525 765 2548 149
Size: 376 240 1783 1968
Fragments arranged by size:
1968 1783 376 240
NarI GG'CG_CC
Cuts at: 1074 1798 2046 1074
Size: 724 248 3395
Fragments arranged by size:
3395 724 248
NcoI C'CATG_G
Cuts at: 725 851 725
Size: 126 4241
NgoMIV G'CCGG_C
Cuts at: 147 523 763 2546 147
Size: 376 240 1783 1968
Fragments arranged by size:
1968 1783 376 240
NspI r_CATG'y
Cuts at: 1420 1920 2827 1420
Size: 500 907 2960
Fragments arranged by size:
2960 907 500
PasI CC'CwG_GG
Cuts at: 506 506
Size: 4367
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PmlI CAC'GTG
Cuts at: 1068 1068
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PstI C_TGCA'G
Cuts at: 392 401 1414 2099 392
Size: 9 1013 685 2660
Fragments arranged by size:
2660 1013 685 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 1792 2152 1792
Size: 360 4007
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SacII CC_GC'GG
Cuts at: 534 534
Size: 4367
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 331 2390 2600 331
Size: 2059 210 2098
Fragments arranged by size:
2098 2059 210
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 388 397 1410 2095 3088 3279 33
Size: 355 9 1013 685 993 191 1121
Fragments arranged by size:
1121 1013 993 685 355 191 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 1243 1243
Size: 4367
SfoI GGC'GCC
Cuts at: 1075 1799 2047 1075
Size: 724 248 3395
Fragments arranged by size:
3395 724 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SgrAI Cr'CCGG_yG
Cuts at: 763 763
Size: 4367
SmaI CCC'GGG
Cuts at: 140 206 1389 140
Size: 66 1183 3118
Fragments arranged by size:
3118 1183 66
SmlI C'TyrA_G
Cuts at: 1293 1522 2437 2929 3191 1293
Size: 229 915 492 262 2469
Fragments arranged by size:
2469 915 492 262 229
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
StyI C'CwwG_G
Cuts at: 293 725 851 1501 1855 293
Size: 432 126 650 354 2805
Fragments arranged by size:
2805 650 432 354 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 270 483 855 1950 2083 2542 2751 270
Size: 213 372 1095 133 459 209 1886
Fragments arranged by size:
1886 1095 459 372 213 209 133
TatI w'GTAC_w
Cuts at: 479 1196 3882 479
Size: 717 2686 964
Fragments arranged by size:
2686 964 717
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1090 1122 1315 1347 3884 3916 1090
Size: 32 193 32 2537 32 1541
Fragments arranged by size:
2537 1541 193 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 264 594 1862 264
Size: 330 1268 2769
Fragments arranged by size:
2769 1268 330
XhoI C'TCGA_G
Cuts at: 1293 1293
Size: 4367
XmaI C'CCGG_G
Cuts at: 138 204 1387 138
Size: 66 1183 3118
Fragments arranged by size:
3118 1183 66
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsiWI
BsmI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI