Restriction map for
pF1KE0245
(Circular) (Six-base) MAPSORT of: pF1KE0245.seq Check: 4638 from: 1 to: 3833
pF1KE0245 3833 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 871 871
Size: 3833
Acc65I G'GTAC_C
Cuts at: 796 849 796
Size: 53 3780
AclI AA'CG_TT
Cuts at: 3673 3673
Size: 3833
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1658 2090 2837 3105 1658
Size: 432 747 268 2386
Fragments arranged by size:
2386 747 432 268
AflIII A'CryG_T
Cuts at: 1185 2289 3266 1185
Size: 1104 977 1752
Fragments arranged by size:
1752 1104 977
AgeI A'CCGG_T
Cuts at: 2221 2221
Size: 3833
AlwNI CAG_nnn'CTG
Cuts at: 1138 2705 1138
Size: 1567 2266
ApaI G_GGCC'C
Cuts at: 504 504
Size: 3833
ApaLI G'TGCA_C
Cuts at: 2603 2603
Size: 3833
ApoI r'AATT_y
Cuts at: 837 3186 3442 837
Size: 2349 256 1228
Fragments arranged by size:
2349 1228 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3833
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
AvaI C'yCGr_G
Cuts at: 215 628 689 853 215
Size: 413 61 164 3195
Fragments arranged by size:
3195 413 164 61
BamHI G'GATC_C
Cuts at: 858 858
Size: 3833
BanI G'GyrC_C
Cuts at: 796 849 1263 1511 1546 796
Size: 53 414 248 35 3083
Fragments arranged by size:
3083 414 248 53 35
BanII G_rGCy'C
Cuts at: 504 847 1877 504
Size: 343 1030 2460
Fragments arranged by size:
2460 1030 343
BbeI G_GCGC'C
Cuts at: 1267 1515 1267
Size: 248 3585
BbvCI CC'TCA_GC
Cuts at: 484 484
Size: 3833
BciVI GTATCCnnnnn_n'
Cuts at: 1731 2492 3833 1731
Size: 761 1341 1731
Fragments arranged by size:
1731 1341 761
BclI T'GATC_A
Cuts at: 1353 1353
Size: 3833
BglI GCCn_nnn'nGGC
Cuts at: 259 575 259
Size: 316 3517
BglII A'GATC_T
Cuts at: 1348 3385 1348
Size: 2037 1796
BlpI GC'TnA_GC
Cuts at: 945 945
Size: 3833
Bme1580I G_kGCm'C
Cuts at: 504 1458 1551 2607 504
Size: 954 93 1056 1730
Fragments arranged by size:
1730 1056 954 93
BmrI ACTGGGnnnn_n'
Cuts at: 1045 1126 1461 3605 1045
Size: 81 335 2144 1273
Fragments arranged by size:
2144 1273 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 3833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 658 754 399
Size: 259 96 3478
Fragments arranged by size:
3478 259 96
Bpu10I CC'TnA_GC
Cuts at: 484 484
Size: 3833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1888 2380 2678 1009
Size: 879 492 298 2164
Fragments arranged by size:
2164 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 235 377 3537 235
Size: 142 3160 531
Fragments arranged by size:
3160 531 142
BsaAI yAC'GTr
Cuts at: 1186 1186
Size: 3833
BsaBI GATnn'nnATC
Cuts at: 6 1347 3384 6
Size: 1341 2037 455
Fragments arranged by size:
2037 1341 455
BsaHI Gr'CG_yC
Cuts at: 297 1264 1512 2214 3710 297
Size: 967 248 702 1496 420
Fragments arranged by size:
1496 967 702 420 248
BsaWI w'CCGG_w
Cuts at: 191 1022 1543 2221 2495 2642 3053 191
Size: 831 521 678 274 147 411 971
Fragments arranged by size:
971 831 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 296 326 523 553 296
Size: 30 197 30 3576
Fragments arranged by size:
3576 197 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 646 127
Size: 519 3314
BseYI C'CCAG_C
Cuts at: 177 387 459 552 573 739 1258 2016 2593
Size: 210 72 93 21 166 519 758 577
Cuts at: 2593 3089 177
Size: 496 921
Fragments arranged by size:
921 758 577 519 496 210 166 93
72 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 274 568 225
Size: 49 294 3490
Fragments arranged by size:
3490 294 49
BsiEI CG_ry'CG
Cuts at: 104 1375 1421 2629 104
Size: 1271 46 1208 1308
Fragments arranged by size:
1308 1271 1208 46
BsiHKAI G_wGCw'C
Cuts at: 847 1625 1815 2607 847
Size: 778 190 792 2073
Fragments arranged by size:
2073 792 778 190
BsiWI C'GTAC_G
Cuts at: 123 123
Size: 3833
Bsp1286I G_dGCh'C
Cuts at: 504 847 1458 1551 1625 1815 1877 2607 504
Size: 343 611 93 74 190 62 730 1730
Fragments arranged by size:
1730 730 611 343 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3053 3053
Size: 3833
BspHI T'CATG_A
Cuts at: 3009 3009
Size: 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 342 883 1097 1399 1780 342
Size: 541 214 302 381 2395
Fragments arranged by size:
2395 541 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2125 2179 3663 3828 50
Size: 2075 54 1484 165 55
Fragments arranged by size:
2075 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1745 1745
Size: 3833
BsrFI r'CCGG_y
Cuts at: 424 1831 2012 2221 424
Size: 1407 181 209 2036
Fragments arranged by size:
2036 1407 209 181
BssSI C'ACGA_G
Cuts at: 171 376 2104 2462 171
Size: 205 1728 358 1542
Fragments arranged by size:
1728 1542 358 205
BstAPI GCAn_nnn'nTGC
Cuts at: 1210 1210
Size: 3833
BstBI TT'CG_AA
Cuts at: 2194 2194
Size: 3833
BstEII G'GTnAC_C
Cuts at: 312 510 312
Size: 198 3635
BstYI r'GATC_y
Cuts at: 1 858 1325 1348 1683 1929 2941 3027 3039
Size: 857 467 23 335 246 1012 86 12
Cuts at: 3039 3385 1
Size: 346 449
Fragments arranged by size:
1012 857 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 475 475
Size: 3833
BtgI C'CryG_G
Cuts at: 107 597 107
Size: 490 3343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1783 1964 2127 1783
Size: 181 163 3489
Fragments arranged by size:
3489 181 163
BtsI GCAGTG_nn'
Cuts at: 213 1076 213
Size: 863 2970
DraI TTT'AAA
Cuts at: 832 832
Size: 3833
DrdI GACnn_nn'nnGTC
Cuts at: 1539 2397 1539
Size: 858 2975
EaeI y'GGCC_r
Cuts at: 260 581 1418 1592 1983 2010 3355 260
Size: 321 837 174 391 27 1345 738
Fragments arranged by size:
1345 837 738 391 321 174 27
EagI C'GGCC_G
Cuts at: 1418 1418
Size: 3833
EarI CTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2041 2351 2497 3643 2041
Size: 310 146 1146 2231
Fragments arranged by size:
2231 1146 310 146
EcoICRI GAG'CTC
Cuts at: 845 845
Size: 3833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 658 754 1658 2090 2837 3105 399
Size: 259 96 904 432 747 268 1127
Fragments arranged by size:
1127 904 747 432 268 259 96
EcoNI CCTnn'n_nnAGG
Cuts at: 474 474
Size: 3833
EcoO109I rG'GnC_Cy
Cuts at: 480 500 501 972 480
Size: 20 1 471 3341
Fragments arranged by size:
3341 471 20 1
EcoRI G'AATT_C
Cuts at: 837 837
Size: 3833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1852 1852
Size: 3833
FspI TGC'GCA
Cuts at: 1614 1614
Size: 3833
HaeII r_GCGC'y
Cuts at: 1267 1515 2537 3516 1267
Size: 248 1022 979 1584
Fragments arranged by size:
1584 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 296 328 355 387 1675 1705 1707 1737 296
Size: 32 27 32 1288 30 2 30 2392
Fragments arranged by size:
2392 1288 32 32 30 30 27 2
HincII GTy'rAC
Cuts at: 384 872 384
Size: 488 3345
KasI G'GCGC_C
Cuts at: 1263 1511 1263
Size: 248 3585
KpnI G_GTAC'C
Cuts at: 800 853 800
Size: 53 3780
MluI A'CGCG_T
Cuts at: 3266 3266
Size: 3833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2504 2688 2504
Size: 184 3649
MscI TGG'CCA
Cuts at: 583 1594 583
Size: 1011 2822
MslI CAynn'nnrTG
Cuts at: 1822 1949 1822
Size: 127 3706
MspA1I CmG'CkG
Cuts at: 288 354 431 441 459 552 573 944 1258
Size: 66 77 10 18 93 21 371 314
Cuts at: 1258 1618 2631 2876 288
Size: 360 1013 245 1245
Fragments arranged by size:
1245 1013 371 360 314 245 93 77
66 21 18 10
NaeI GCC'GGC
Cuts at: 2014 2014
Size: 3833
NarI GG'CG_CC
Cuts at: 1264 1512 1264
Size: 248 3585
NcoI C'CATG_G
Cuts at: 107 107
Size: 3833
NgoMIV G'CCGG_C
Cuts at: 2012 2012
Size: 3833
NspI r_CATG'y
Cuts at: 886 1386 2293 886
Size: 500 907 2426
Fragments arranged by size:
2426 907 500
PciI A'CATG_T
Cuts at: 2289 2289
Size: 3833
PmeI GTTT'AAAC
Cuts at: 832 832
Size: 3833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2972 3004 2972
Size: 32 3801
PspOMI G'GGCC_C
Cuts at: 500 500
Size: 3833
PstI C_TGCA'G
Cuts at: 287 339 880 1565 287
Size: 52 541 685 2555
Fragments arranged by size:
2555 685 541 52
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3833
PvuII CAG'CTG
Cuts at: 288 354 459 552 1258 1618 288
Size: 66 105 93 706 360 2503
Fragments arranged by size:
2503 706 360 105 93 66
RsrII CG'GwC_CG
Cuts at: 2028 2028
Size: 3833
SacI G_AGCT'C
Cuts at: 847 847
Size: 3833
SalI G'TCGA_C
Cuts at: 870 870
Size: 3833
SapI GCTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
SbfI CC_TGCA'GG
Cuts at: 339 880 339
Size: 541 3292
ScaI AGT'ACT
Cuts at: 3350 3350
Size: 3833
SfcI C'TryA_G
Cuts at: 33 283 335 876 1561 2554 2745 33
Size: 250 52 541 685 993 191 1121
Fragments arranged by size:
1121 993 685 541 250 191 52
SfiI GGCCn_nnn'nGGCC
Cuts at: 259 259
Size: 3833
SfoI GGC'GCC
Cuts at: 1265 1513 1265
Size: 248 3585
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
SmaI CCC'GGG
Cuts at: 217 691 855 217
Size: 474 164 3195
Fragments arranged by size:
3195 474 164
SmlI C'TyrA_G
Cuts at: 988 1903 2395 2657 988
Size: 915 492 262 2164
Fragments arranged by size:
2164 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3833
SphI G_CATG'C
Cuts at: 886 1386 886
Size: 500 3333
StyI C'CwwG_G
Cuts at: 107 267 542 967 1321 107
Size: 160 275 425 354 2619
Fragments arranged by size:
2619 425 354 275 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 172 610 706 1416 1549 2008 2217 172
Size: 438 96 710 133 459 209 1788
Fragments arranged by size:
1788 710 459 438 209 133 96
TatI w'GTAC_w
Cuts at: 3348 3348
Size: 3833
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3350 3382 3350
Size: 32 3801
Tth111I GACn'n_nGTC
Cuts at: 1630 1630
Size: 3833
XbaI T'CTAG_A
Cuts at: 864 864
Size: 3833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 549 1328 549
Size: 779 3054
XmaI C'CCGG_G
Cuts at: 215 689 853 215
Size: 474 164 3195
Fragments arranged by size:
3195 474 164
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BsmI BsmBI BsrGI BssHII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI