Restriction map for
pF1KE0236
(Circular) (Six-base) MAPSORT of: pF1KE0236.seq Check: 6347 from: 1 to: 3941
pF1KE0236 3941 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 979 979
Size: 3941
Acc65I G'GTAC_C
Cuts at: 957 957
Size: 3941
AclI AA'CG_TT
Cuts at: 499 3781 499
Size: 3282 659
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 1766 2198 2945 3213 600
Size: 1166 432 747 268 1328
Fragments arranged by size:
1328 1166 747 432 268
AflIII A'CryG_T
Cuts at: 1293 2397 3374 1293
Size: 1104 977 1860
Fragments arranged by size:
1860 1104 977
AgeI A'CCGG_T
Cuts at: 2329 2329
Size: 3941
AlwNI CAG_nnn'CTG
Cuts at: 1246 2813 1246
Size: 1567 2374
ApaI G_GGCC'C
Cuts at: 673 673
Size: 3941
ApaLI G'TGCA_C
Cuts at: 2711 2711
Size: 3941
ApoI r'AATT_y
Cuts at: 111 945 3294 3550 111
Size: 834 2349 256 502
Fragments arranged by size:
2349 834 502 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3941
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
AvaI C'yCGr_G
Cuts at: 961 961
Size: 3941
BamHI G'GATC_C
Cuts at: 966 966
Size: 3941
BanI G'GyrC_C
Cuts at: 520 957 1371 1619 1654 520
Size: 437 414 248 35 2807
Fragments arranged by size:
2807 437 414 248 35
BanII G_rGCy'C
Cuts at: 485 673 955 1985 485
Size: 188 282 1030 2441
Fragments arranged by size:
2441 1030 282 188
BbeI G_GCGC'C
Cuts at: 1375 1623 1375
Size: 248 3693
BbsI GAAGACnn'nnnn_
Cuts at: 630 630
Size: 3941
BbvCI CC'TCA_GC
Cuts at: 747 747
Size: 3941
BciVI GTATCCnnnnn_n'
Cuts at: 1839 2600 3941 1839
Size: 761 1341 1839
Fragments arranged by size:
1839 1341 761
BclI T'GATC_A
Cuts at: 1461 1461
Size: 3941
BglII A'GATC_T
Cuts at: 1456 3493 1456
Size: 2037 1904
BlpI GC'TnA_GC
Cuts at: 1053 1053
Size: 3941
Bme1580I G_kGCm'C
Cuts at: 523 673 1566 1659 2715 523
Size: 150 893 93 1056 1749
Fragments arranged by size:
1749 1056 893 150 93
BmrI ACTGGGnnnn_n'
Cuts at: 479 1153 1234 1569 3713 479
Size: 674 81 335 2144 707
Fragments arranged by size:
2144 707 674 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 3941
Bpu10I CC'TnA_GC
Cuts at: 747 747
Size: 3941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1117 1996 2488 2786 1117
Size: 879 492 298 2272
Fragments arranged by size:
2272 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 655 3645 655
Size: 2990 951
BsaAI yAC'GTr
Cuts at: 1294 1294
Size: 3941
BsaBI GATnn'nnATC
Cuts at: 6 1455 3492 6
Size: 1449 2037 455
Fragments arranged by size:
2037 1449 455
BsaHI Gr'CG_yC
Cuts at: 455 1372 1620 2322 3818 455
Size: 917 248 702 1496 578
Fragments arranged by size:
1496 917 702 578 248
BsaWI w'CCGG_w
Cuts at: 1130 1651 2329 2603 2750 3161 1130
Size: 521 678 274 147 411 1910
Fragments arranged by size:
1910 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 388 750 388
Size: 362 3579
BseYI C'CCAG_C
Cuts at: 683 1366 2124 2701 3197 683
Size: 683 758 577 496 1427
Fragments arranged by size:
1427 758 683 577 496
BsiEI CG_ry'CG
Cuts at: 104 1483 1529 2737 104
Size: 1379 46 1208 1308
Fragments arranged by size:
1379 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 955 1733 1923 2715 955
Size: 778 190 792 2181
Fragments arranged by size:
2181 792 778 190
BsmI GAATG_Cn'
Cuts at: 128 128
Size: 3941
Bsp1286I G_dGCh'C
Cuts at: 485 523 673 955 1566 1659 1733 1923 1985
Size: 38 150 282 611 93 74 190 62
Cuts at: 1985 2715 485
Size: 730 1711
Fragments arranged by size:
1711 730 611 282 190 150 93 74
62 38
BspEI T'CCGG_A
Cuts at: 3161 3161
Size: 3941
BspHI T'CATG_A
Cuts at: 416 861 3117 416
Size: 445 2256 1240
Fragments arranged by size:
2256 1240 445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 353 991 1205 1507 1888 353
Size: 638 214 302 381 2406
Fragments arranged by size:
2406 638 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2233 2287 3771 3936 50
Size: 2183 54 1484 165 55
Fragments arranged by size:
2183 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1853 1853
Size: 3941
BsrFI r'CCGG_y
Cuts at: 1939 2120 2329 1939
Size: 181 209 3551
Fragments arranged by size:
3551 209 181
BssSI C'ACGA_G
Cuts at: 2212 2570 2212
Size: 358 3583
BstAPI GCAn_nnn'nTGC
Cuts at: 1318 1318
Size: 3941
BstBI TT'CG_AA
Cuts at: 495 2302 495
Size: 1807 2134
BstYI r'GATC_y
Cuts at: 1 966 1433 1456 1791 2037 3049 3135 3147
Size: 965 467 23 335 246 1012 86 12
Cuts at: 3147 3493 1
Size: 346 449
Fragments arranged by size:
1012 965 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 382 382
Size: 3941
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 564 1891 2072 2235 564
Size: 1327 181 163 2270
Fragments arranged by size:
2270 1327 181 163
BtsI GCAGTG_nn'
Cuts at: 1184 1184
Size: 3941
DraI TTT'AAA
Cuts at: 513 940 513
Size: 427 3514
DrdI GACnn_nn'nnGTC
Cuts at: 298 1647 2505 298
Size: 1349 858 1734
Fragments arranged by size:
1734 1349 858
EaeI y'GGCC_r
Cuts at: 344 1526 1700 2091 2118 3463 344
Size: 1182 174 391 27 1345 822
Fragments arranged by size:
1345 1182 822 391 174 27
EagI C'GGCC_G
Cuts at: 1526 1526
Size: 3941
EarI CTCTTCn'nnn_
Cuts at: 497 556 619 1964 2174 497
Size: 59 63 1345 210 2264
Fragments arranged by size:
2264 1345 210 63 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 339 2149 2459 2605 3751 339
Size: 1810 310 146 1146 529
Fragments arranged by size:
1810 1146 529 310 146
EcoICRI GAG'CTC
Cuts at: 953 953
Size: 3941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 1766 2198 2945 3213 600
Size: 1166 432 747 268 1328
Fragments arranged by size:
1328 1166 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 300 1080 300
Size: 780 3161
EcoRI G'AATT_C
Cuts at: 111 945 111
Size: 834 3107
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1960 1960
Size: 3941
FspI TGC'GCA
Cuts at: 1722 1722
Size: 3941
HaeII r_GCGC'y
Cuts at: 1375 1623 2645 3624 1375
Size: 248 1022 979 1692
Fragments arranged by size:
1692 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1783 1813 1815 1845 1783
Size: 30 2 30 3879
Fragments arranged by size:
3879 30 30 2
HincII GTy'rAC
Cuts at: 980 980
Size: 3941
KasI G'GCGC_C
Cuts at: 1371 1619 1371
Size: 248 3693
KpnI G_GTAC'C
Cuts at: 961 961
Size: 3941
MluI A'CGCG_T
Cuts at: 3374 3374
Size: 3941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2612 2796 2612
Size: 184 3757
MscI TGG'CCA
Cuts at: 1702 1702
Size: 3941
MslI CAynn'nnrTG
Cuts at: 448 1930 2057 448
Size: 1482 127 2332
Fragments arranged by size:
2332 1482 127
MspA1I CmG'CkG
Cuts at: 1052 1366 1726 2739 2984 1052
Size: 314 360 1013 245 2009
Fragments arranged by size:
2009 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2122 2122
Size: 3941
NarI GG'CG_CC
Cuts at: 1372 1620 1372
Size: 248 3693
NgoMIV G'CCGG_C
Cuts at: 2120 2120
Size: 3941
NspI r_CATG'y
Cuts at: 559 994 1494 2401 559
Size: 435 500 907 2099
Fragments arranged by size:
2099 907 500 435
PasI CC'CwG_GG
Cuts at: 666 666
Size: 3941
PciI A'CATG_T
Cuts at: 2397 2397
Size: 3941
PfoI T'CCnGG_A
Cuts at: 302 593 302
Size: 291 3650
PmeI GTTT'AAAC
Cuts at: 940 940
Size: 3941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 188 220 3080 3112 188
Size: 32 2860 32 1017
Fragments arranged by size:
2860 1017 32 32
PpuMI rG'GwC_Cy
Cuts at: 300 300
Size: 3941
PsiI TTA'TAA
Cuts at: 244 244
Size: 3941
PspOMI G'GGCC_C
Cuts at: 669 669
Size: 3941
PstI C_TGCA'G
Cuts at: 988 1673 988
Size: 685 3256
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3941
PvuII CAG'CTG
Cuts at: 1366 1726 1366
Size: 360 3581
RsrII CG'GwC_CG
Cuts at: 2136 2136
Size: 3941
SacI G_AGCT'C
Cuts at: 955 955
Size: 3941
SalI G'TCGA_C
Cuts at: 978 978
Size: 3941
SapI GCTCTTCn'nnn_
Cuts at: 556 1964 2174 556
Size: 1408 210 2323
Fragments arranged by size:
2323 1408 210
SbfI CC_TGCA'GG
Cuts at: 988 988
Size: 3941
ScaI AGT'ACT
Cuts at: 3458 3458
Size: 3941
SexAI A'CCwGG_T
Cuts at: 632 632
Size: 3941
SfcI C'TryA_G
Cuts at: 33 984 1669 2662 2853 33
Size: 951 685 993 191 1121
Fragments arranged by size:
1121 993 951 685 191
SfoI GGC'GCC
Cuts at: 1373 1621 1373
Size: 248 3693
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
SmaI CCC'GGG
Cuts at: 963 963
Size: 3941
SmlI C'TyrA_G
Cuts at: 1096 2011 2503 2765 1096
Size: 915 492 262 2272
Fragments arranged by size:
2272 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3941
SphI G_CATG'C
Cuts at: 994 1494 994
Size: 500 3441
SspI AAT'ATT
Cuts at: 822 913 822
Size: 91 3850
StyI C'CwwG_G
Cuts at: 1075 1429 1075
Size: 354 3587
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 559 1524 1657 2116 2325 559
Size: 965 133 459 209 2175
Fragments arranged by size:
2175 965 459 209 133
TatI w'GTAC_w
Cuts at: 3456 3456
Size: 3941
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 3909
Tth111I GACn'n_nGTC
Cuts at: 1738 1738
Size: 3941
XbaI T'CTAG_A
Cuts at: 972 972
Size: 3941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1436 1436
Size: 3941
XmaI C'CCGG_G
Cuts at: 961 961
Size: 3941
XmnI GAAnn'nnTTC
Cuts at: 197 649 197
Size: 452 3489
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI