Restriction map for
pF1KE0228
(Circular) (Six-base) MAPSORT of: pF1KE0228.seq Check: 9805 from: 1 to: 3443
pF1KE0228 3443 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 481 481
Size: 3443
Acc65I G'GTAC_C
Cuts at: 459 459
Size: 3443
AclI AA'CG_TT
Cuts at: 3283 3283
Size: 3443
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 1268 1700 2447 2715 145
Size: 1123 432 747 268 873
Fragments arranged by size:
1123 873 747 432 268
AflIII A'CryG_T
Cuts at: 795 1899 2876 795
Size: 1104 977 1362
Fragments arranged by size:
1362 1104 977
AgeI A'CCGG_T
Cuts at: 1831 1831
Size: 3443
AlwNI CAG_nnn'CTG
Cuts at: 748 2315 748
Size: 1567 1876
ApaLI G'TGCA_C
Cuts at: 2213 2213
Size: 3443
ApoI r'AATT_y
Cuts at: 317 447 2796 3052 317
Size: 130 2349 256 708
Fragments arranged by size:
2349 708 256 130
AseI AT'TA_AT
Cuts at: 20 20
Size: 3443
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3443
AvaI C'yCGr_G
Cuts at: 269 463 269
Size: 194 3249
BamHI G'GATC_C
Cuts at: 468 468
Size: 3443
BanI G'GyrC_C
Cuts at: 459 873 1121 1156 459
Size: 414 248 35 2746
Fragments arranged by size:
2746 414 248 35
BanII G_rGCy'C
Cuts at: 306 457 1487 306
Size: 151 1030 2262
Fragments arranged by size:
2262 1030 151
BbeI G_GCGC'C
Cuts at: 877 1125 877
Size: 248 3195
BbsI GAAGACnn'nnnn_
Cuts at: 350 350
Size: 3443
BciVI GTATCCnnnnn_n'
Cuts at: 1341 2102 3443 1341
Size: 761 1341 1341
Fragments arranged by size:
1341 1341 761
BclI T'GATC_A
Cuts at: 963 963
Size: 3443
BglII A'GATC_T
Cuts at: 958 2995 958
Size: 2037 1406
BlpI GC'TnA_GC
Cuts at: 211 555 211
Size: 344 3099
Bme1580I G_kGCm'C
Cuts at: 1068 1161 2217 1068
Size: 93 1056 2294
Fragments arranged by size:
2294 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 193 655 736 1071 3215 193
Size: 462 81 335 2144 421
Fragments arranged by size:
2144 462 421 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1810 1810
Size: 3443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 290
Size: 3443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 1498 1990 2288 619
Size: 879 492 298 1774
Fragments arranged by size:
1774 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3147 3147
Size: 3443
BsaAI yAC'GTr
Cuts at: 796 796
Size: 3443
BsaBI GATnn'nnATC
Cuts at: 6 957 2994 6
Size: 951 2037 455
Fragments arranged by size:
2037 951 455
BsaHI Gr'CG_yC
Cuts at: 874 1122 1824 3320 874
Size: 248 702 1496 997
Fragments arranged by size:
1496 997 702 248
BsaWI w'CCGG_w
Cuts at: 632 1153 1831 2105 2252 2663 632
Size: 521 678 274 147 411 1412
Fragments arranged by size:
1412 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 366 868 1626 2203 2699 366
Size: 502 758 577 496 1110
Fragments arranged by size:
1110 758 577 502 496
BsiEI CG_ry'CG
Cuts at: 104 985 1031 2239 104
Size: 881 46 1208 1308
Fragments arranged by size:
1308 1208 881 46
BsiHKAI G_wGCw'C
Cuts at: 457 1235 1425 2217 457
Size: 778 190 792 1683
Fragments arranged by size:
1683 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 306 457 1068 1161 1235 1425 1487 2217 306
Size: 151 611 93 74 190 62 730 1532
Fragments arranged by size:
1532 730 611 190 151 93 74 62
BspEI T'CCGG_A
Cuts at: 2663 2663
Size: 3443
BspHI T'CATG_A
Cuts at: 2619 2619
Size: 3443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 370 493 707 1009 1390 370
Size: 123 214 302 381 2423
Fragments arranged by size:
2423 381 302 214 123
BsrBI CCG'CTC
Cuts at: 50 1735 1789 3273 3438 50
Size: 1685 54 1484 165 55
Fragments arranged by size:
1685 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1355 1355
Size: 3443
BsrFI r'CCGG_y
Cuts at: 1441 1622 1831 1441
Size: 181 209 3053
Fragments arranged by size:
3053 209 181
BssSI C'ACGA_G
Cuts at: 1714 2072 1714
Size: 358 3085
BstAPI GCAn_nnn'nTGC
Cuts at: 820 820
Size: 3443
BstBI TT'CG_AA
Cuts at: 1804 1804
Size: 3443
BstYI r'GATC_y
Cuts at: 1 468 935 958 1293 1539 2551 2637 2649
Size: 467 467 23 335 246 1012 86 12
Cuts at: 2649 2995 1
Size: 346 449
Fragments arranged by size:
1012 467 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3443
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1393 1574 1737 1393
Size: 181 163 3099
Fragments arranged by size:
3099 181 163
BtsI GCAGTG_nn'
Cuts at: 686 686
Size: 3443
DraI TTT'AAA
Cuts at: 442 442
Size: 3443
DrdI GACnn_nn'nnGTC
Cuts at: 1149 2007 1149
Size: 858 2585
EaeI y'GGCC_r
Cuts at: 110 1028 1202 1593 1620 2965 110
Size: 918 174 391 27 1345 588
Fragments arranged by size:
1345 918 588 391 174 27
EagI C'GGCC_G
Cuts at: 1028 1028
Size: 3443
EarI CTCTTCn'nnn_
Cuts at: 127 215 1466 1676 127
Size: 88 1251 210 1894
Fragments arranged by size:
1894 1251 210 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1651 1961 2107 3253 1651
Size: 310 146 1146 1841
Fragments arranged by size:
1841 1146 310 146
EcoICRI GAG'CTC
Cuts at: 455 455
Size: 3443
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 290 1268 1700 2447 2715 145
Size: 145 978 432 747 268 873
Fragments arranged by size:
978 873 747 432 268 145
EcoO109I rG'GnC_Cy
Cuts at: 120 582 120
Size: 462 2981
EcoRI G'AATT_C
Cuts at: 447 447
Size: 3443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1462 1462
Size: 3443
FspI TGC'GCA
Cuts at: 1224 1224
Size: 3443
HaeII r_GCGC'y
Cuts at: 877 1125 2147 3126 877
Size: 248 1022 979 1194
Fragments arranged by size:
1194 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1285 1315 1317 1347 1285
Size: 30 2 30 3381
Fragments arranged by size:
3381 30 30 2
HincII GTy'rAC
Cuts at: 482 482
Size: 3443
KasI G'GCGC_C
Cuts at: 873 1121 873
Size: 248 3195
KpnI G_GTAC'C
Cuts at: 463 463
Size: 3443
MluI A'CGCG_T
Cuts at: 2876 2876
Size: 3443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 409 2114 2298 409
Size: 1705 184 1554
Fragments arranged by size:
1705 1554 184
MscI TGG'CCA
Cuts at: 1204 1204
Size: 3443
MslI CAynn'nnrTG
Cuts at: 1432 1559 1432
Size: 127 3316
MspA1I CmG'CkG
Cuts at: 554 868 1228 2241 2486 554
Size: 314 360 1013 245 1511
Fragments arranged by size:
1511 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1624 1624
Size: 3443
NarI GG'CG_CC
Cuts at: 874 1122 874
Size: 248 3195
NcoI C'CATG_G
Cuts at: 107 107
Size: 3443
NgoMIV G'CCGG_C
Cuts at: 1622 1622
Size: 3443
NspI r_CATG'y
Cuts at: 314 496 996 1903 314
Size: 182 500 907 1854
Fragments arranged by size:
1854 907 500 182
PciI A'CATG_T
Cuts at: 1899 1899
Size: 3443
PflMI CCAn_nnn'nTGG
Cuts at: 391 391
Size: 3443
PmeI GTTT'AAAC
Cuts at: 442 442
Size: 3443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2582 2614 2582
Size: 32 3411
PpuMI rG'GwC_Cy
Cuts at: 120 120
Size: 3443
PstI C_TGCA'G
Cuts at: 490 1175 490
Size: 685 2758
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3443
PvuII CAG'CTG
Cuts at: 868 1228 868
Size: 360 3083
RsrII CG'GwC_CG
Cuts at: 1638 1638
Size: 3443
SacI G_AGCT'C
Cuts at: 457 457
Size: 3443
SalI G'TCGA_C
Cuts at: 480 480
Size: 3443
SapI GCTCTTCn'nnn_
Cuts at: 1466 1676 1466
Size: 210 3233
SbfI CC_TGCA'GG
Cuts at: 490 490
Size: 3443
ScaI AGT'ACT
Cuts at: 428 2960 428
Size: 2532 911
SfcI C'TryA_G
Cuts at: 33 251 486 1171 2164 2355 33
Size: 218 235 685 993 191 1121
Fragments arranged by size:
1121 993 685 235 218 191
SfoI GGC'GCC
Cuts at: 875 1123 875
Size: 248 3195
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3443
SmaI CCC'GGG
Cuts at: 465 465
Size: 3443
SmlI C'TyrA_G
Cuts at: 598 1513 2005 2267 598
Size: 915 492 262 1774
Fragments arranged by size:
1774 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3443
SphI G_CATG'C
Cuts at: 314 496 996 314
Size: 182 500 2761
Fragments arranged by size:
2761 500 182
StyI C'CwwG_G
Cuts at: 107 577 931 107
Size: 470 354 2619
Fragments arranged by size:
2619 470 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 175 1026 1159 1618 1827 175
Size: 851 133 459 209 1791
Fragments arranged by size:
1791 851 459 209 133
TatI w'GTAC_w
Cuts at: 426 2958 426
Size: 2532 911
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2960 2992 2960
Size: 32 3411
Tth111I GACn'n_nGTC
Cuts at: 118 1240 118
Size: 1122 2321
XbaI T'CTAG_A
Cuts at: 474 474
Size: 3443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 938 938
Size: 3443
XmaI C'CCGG_G
Cuts at: 463 463
Size: 3443
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI