Restriction map for
pF1KE0224
(Circular) (Six-base) MAPSORT of: pF1KE0224.seq Check: 2215 from: 1 to: 3380
pF1KE0224 3380 bp
With 182 enzymes: SgfI *
July 23, 2008 09:45 ..
AccI GT'mk_AC
Cuts at: 418 418
Size: 3380
Acc65I G'GTAC_C
Cuts at: 396 396
Size: 3380
AclI AA'CG_TT
Cuts at: 3220 3220
Size: 3380
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 1205 1637 2384 2652 139
Size: 1066 432 747 268 867
Fragments arranged by size:
1066 867 747 432 268
AflIII A'CryG_T
Cuts at: 223 732 1836 2813 223
Size: 509 1104 977 790
Fragments arranged by size:
1104 977 790 509
AgeI A'CCGG_T
Cuts at: 1768 1768
Size: 3380
AlwNI CAG_nnn'CTG
Cuts at: 240 685 2252 240
Size: 445 1567 1368
Fragments arranged by size:
1567 1368 445
ApaLI G'TGCA_C
Cuts at: 2150 2150
Size: 3380
ApoI r'AATT_y
Cuts at: 246 384 2733 2989 246
Size: 138 2349 256 637
Fragments arranged by size:
2349 637 256 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 3380
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3380
AvaI C'yCGr_G
Cuts at: 400 400
Size: 3380
BamHI G'GATC_C
Cuts at: 304 405 304
Size: 101 3279
BanI G'GyrC_C
Cuts at: 277 396 810 1058 1093 277
Size: 119 414 248 35 2564
Fragments arranged by size:
2564 414 248 119 35
BanII G_rGCy'C
Cuts at: 394 1424 394
Size: 1030 2350
BbeI G_GCGC'C
Cuts at: 814 1062 814
Size: 248 3132
BciVI GTATCCnnnnn_n'
Cuts at: 1278 2039 3380 1278
Size: 761 1341 1278
Fragments arranged by size:
1341 1278 761
BclI T'GATC_A
Cuts at: 900 900
Size: 3380
BglII A'GATC_T
Cuts at: 895 2932 895
Size: 2037 1343
BlpI GC'TnA_GC
Cuts at: 492 492
Size: 3380
Bme1580I G_kGCm'C
Cuts at: 1005 1098 2154 1005
Size: 93 1056 2231
Fragments arranged by size:
2231 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 592 673 1008 3152 592
Size: 81 335 2144 820
Fragments arranged by size:
2144 820 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1747 1747
Size: 3380
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1435 1927 2225 556
Size: 879 492 298 1711
Fragments arranged by size:
1711 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3084 3084
Size: 3380
BsaAI yAC'GTr
Cuts at: 733 733
Size: 3380
BsaBI GATnn'nnATC
Cuts at: 6 894 2931 6
Size: 888 2037 455
Fragments arranged by size:
2037 888 455
BsaHI Gr'CG_yC
Cuts at: 811 1059 1761 3257 811
Size: 248 702 1496 934
Fragments arranged by size:
1496 934 702 248
BsaWI w'CCGG_w
Cuts at: 569 1090 1768 2042 2189 2600 569
Size: 521 678 274 147 411 1349
Fragments arranged by size:
1349 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 805 1563 2140 2636 805
Size: 758 577 496 1549
Fragments arranged by size:
1549 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 922 968 2176 104
Size: 818 46 1208 1308
Fragments arranged by size:
1308 1208 818 46
BsiHKAI G_wGCw'C
Cuts at: 146 394 1172 1362 2154 146
Size: 248 778 190 792 1372
Fragments arranged by size:
1372 792 778 248 190
BsmI GAATG_Cn'
Cuts at: 333 333
Size: 3380
Bsp1286I G_dGCh'C
Cuts at: 146 394 1005 1098 1172 1362 1424 2154 146
Size: 248 611 93 74 190 62 730 1372
Fragments arranged by size:
1372 730 611 248 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2600 2600
Size: 3380
BspHI T'CATG_A
Cuts at: 2556 2556
Size: 3380
BspMI ACCTGCnnnn'nnnn_
Cuts at: 430 644 946 1327 430
Size: 214 302 381 2483
Fragments arranged by size:
2483 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1672 1726 3210 3375 50
Size: 1622 54 1484 165 55
Fragments arranged by size:
1622 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1292 1292
Size: 3380
BsrFI r'CCGG_y
Cuts at: 1378 1559 1768 1378
Size: 181 209 2990
Fragments arranged by size:
2990 209 181
BssSI C'ACGA_G
Cuts at: 1651 2009 1651
Size: 358 3022
BstAPI GCAn_nnn'nTGC
Cuts at: 757 757
Size: 3380
BstBI TT'CG_AA
Cuts at: 1741 1741
Size: 3380
BstYI r'GATC_y
Cuts at: 1 304 405 872 895 1230 1476 2488 2574
Size: 303 101 467 23 335 246 1012 86
Cuts at: 2574 2586 2932 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 303 246 101
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3380
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1330 1511 1674 1330
Size: 181 163 3036
Fragments arranged by size:
3036 181 163
BtsI GCAGTG_nn'
Cuts at: 215 623 215
Size: 408 2972
DraI TTT'AAA
Cuts at: 344 379 344
Size: 35 3345
DrdI GACnn_nn'nnGTC
Cuts at: 1086 1944 1086
Size: 858 2522
EaeI y'GGCC_r
Cuts at: 965 1139 1530 1557 2902 965
Size: 174 391 27 1345 1443
Fragments arranged by size:
1443 1345 391 174 27
EagI C'GGCC_G
Cuts at: 965 965
Size: 3380
EarI CTCTTCn'nnn_
Cuts at: 1403 1613 1403
Size: 210 3170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1588 1898 2044 3190 1588
Size: 310 146 1146 1778
Fragments arranged by size:
1778 1146 310 146
EcoICRI GAG'CTC
Cuts at: 392 392
Size: 3380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 1205 1637 2384 2652 139
Size: 1066 432 747 268 867
Fragments arranged by size:
1066 867 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 519 519
Size: 3380
EcoRI G'AATT_C
Cuts at: 384 384
Size: 3380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1399 1399
Size: 3380
FspI TGC'GCA
Cuts at: 1161 1161
Size: 3380
HaeII r_GCGC'y
Cuts at: 240 814 1062 2084 3063 240
Size: 574 248 1022 979 557
Fragments arranged by size:
1022 979 574 557 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1222 1252 1254 1284 1222
Size: 30 2 30 3318
Fragments arranged by size:
3318 30 30 2
HincII GTy'rAC
Cuts at: 419 419
Size: 3380
KasI G'GCGC_C
Cuts at: 810 1058 810
Size: 248 3132
KpnI G_GTAC'C
Cuts at: 400 400
Size: 3380
MluI A'CGCG_T
Cuts at: 2813 2813
Size: 3380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2235 2051
Size: 184 3196
MscI TGG'CCA
Cuts at: 1141 1141
Size: 3380
MslI CAynn'nnrTG
Cuts at: 1369 1496 1369
Size: 127 3253
MspA1I CmG'CkG
Cuts at: 491 805 1165 2178 2423 491
Size: 314 360 1013 245 1448
Fragments arranged by size:
1448 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1561 1561
Size: 3380
NarI GG'CG_CC
Cuts at: 811 1059 811
Size: 248 3132
NcoI C'CATG_G
Cuts at: 107 107
Size: 3380
NdeI CA'TA_TG
Cuts at: 315 315
Size: 3380
NgoMIV G'CCGG_C
Cuts at: 1559 1559
Size: 3380
NspI r_CATG'y
Cuts at: 227 433 933 1840 227
Size: 206 500 907 1767
Fragments arranged by size:
1767 907 500 206
PciI A'CATG_T
Cuts at: 223 1836 223
Size: 1613 1767
PmeI GTTT'AAAC
Cuts at: 379 379
Size: 3380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2519 2551 2519
Size: 32 3348
PstI C_TGCA'G
Cuts at: 427 1112 427
Size: 685 2695
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3380
PvuII CAG'CTG
Cuts at: 805 1165 805
Size: 360 3020
RsrII CG'GwC_CG
Cuts at: 1575 1575
Size: 3380
SacI G_AGCT'C
Cuts at: 394 394
Size: 3380
SalI G'TCGA_C
Cuts at: 417 417
Size: 3380
SapI GCTCTTCn'nnn_
Cuts at: 1403 1613 1403
Size: 210 3170
SbfI CC_TGCA'GG
Cuts at: 427 427
Size: 3380
ScaI AGT'ACT
Cuts at: 2897 2897
Size: 3380
SfcI C'TryA_G
Cuts at: 33 423 1108 2101 2292 33
Size: 390 685 993 191 1121
Fragments arranged by size:
1121 993 685 390 191
SfoI GGC'GCC
Cuts at: 812 1060 812
Size: 248 3132
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3380
SmaI CCC'GGG
Cuts at: 402 402
Size: 3380
SmlI C'TyrA_G
Cuts at: 535 1450 1942 2204 535
Size: 915 492 262 1711
Fragments arranged by size:
1711 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3380
SphI G_CATG'C
Cuts at: 433 933 433
Size: 500 2880
StyI C'CwwG_G
Cuts at: 107 514 868 107
Size: 407 354 2619
Fragments arranged by size:
2619 407 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 192 963 1096 1555 1764 192
Size: 771 133 459 209 1808
Fragments arranged by size:
1808 771 459 209 133
TatI w'GTAC_w
Cuts at: 2895 2895
Size: 3380
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 3348
Tth111I GACn'n_nGTC
Cuts at: 1177 1177
Size: 3380
XbaI T'CTAG_A
Cuts at: 411 411
Size: 3380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 875 875
Size: 3380
XmaI C'CCGG_G
Cuts at: 400 400
Size: 3380
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI