Restriction map for
pF1KE0219
(Circular) (Six-base) MAPSORT of: pF1KE0219.seq Check: 1965 from: 1 to: 3599
pF1KE0219 3599 bp
With 182 enzymes: SgfI *
October 6, 2008 10:37 ..
AccI GT'mk_AC
Cuts at: 637 637
Size: 3599
Acc65I G'GTAC_C
Cuts at: 615 615
Size: 3599
AclI AA'CG_TT
Cuts at: 3439 3439
Size: 3599
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 1424 1856 2603 2871 473
Size: 951 432 747 268 1201
Fragments arranged by size:
1201 951 747 432 268
AflIII A'CryG_T
Cuts at: 951 2055 3032 951
Size: 1104 977 1518
Fragments arranged by size:
1518 1104 977
AgeI A'CCGG_T
Cuts at: 1987 1987
Size: 3599
AlwNI CAG_nnn'CTG
Cuts at: 904 2471 904
Size: 1567 2032
ApaLI G'TGCA_C
Cuts at: 2369 2369
Size: 3599
ApoI r'AATT_y
Cuts at: 410 603 2952 3208 410
Size: 193 2349 256 801
Fragments arranged by size:
2349 801 256 193
AseI AT'TA_AT
Cuts at: 20 20
Size: 3599
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3599
AvaI C'yCGr_G
Cuts at: 181 196 619 181
Size: 15 423 3161
Fragments arranged by size:
3161 423 15
BamHI G'GATC_C
Cuts at: 228 624 228
Size: 396 3203
BanI G'GyrC_C
Cuts at: 615 1029 1277 1312 615
Size: 414 248 35 2902
Fragments arranged by size:
2902 414 248 35
BanII G_rGCy'C
Cuts at: 613 1643 613
Size: 1030 2569
BbeI G_GCGC'C
Cuts at: 1033 1281 1033
Size: 248 3351
BbvCI CC'TCA_GC
Cuts at: 427 427
Size: 3599
BciVI GTATCCnnnnn_n'
Cuts at: 1497 2258 3599 1497
Size: 761 1341 1497
Fragments arranged by size:
1497 1341 761
BclI T'GATC_A
Cuts at: 1119 1119
Size: 3599
BglII A'GATC_T
Cuts at: 1114 3151 1114
Size: 2037 1562
BlpI GC'TnA_GC
Cuts at: 711 711
Size: 3599
Bme1580I G_kGCm'C
Cuts at: 1224 1317 2373 1224
Size: 93 1056 2450
Fragments arranged by size:
2450 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 811 892 1227 3371 811
Size: 81 335 2144 1039
Fragments arranged by size:
2144 1039 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1966 1966
Size: 3599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 136
Size: 3599
Bpu10I CC'TnA_GC
Cuts at: 427 427
Size: 3599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 775 1654 2146 2444 238
Size: 537 879 492 298 1393
Fragments arranged by size:
1393 879 537 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3303 3303
Size: 3599
BsaAI yAC'GTr
Cuts at: 354 952 354
Size: 598 3001
BsaBI GATnn'nnATC
Cuts at: 6 1113 3150 6
Size: 1107 2037 455
Fragments arranged by size:
2037 1107 455
BsaHI Gr'CG_yC
Cuts at: 1030 1278 1980 3476 1030
Size: 248 702 1496 1153
Fragments arranged by size:
1496 1153 702 248
BsaWI w'CCGG_w
Cuts at: 788 1309 1987 2261 2408 2819 788
Size: 521 678 274 147 411 1568
Fragments arranged by size:
1568 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 142 172 142
Size: 30 3569
BseYI C'CCAG_C
Cuts at: 258 1024 1782 2359 2855 258
Size: 766 758 577 496 1002
Fragments arranged by size:
1002 766 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 514 421
Size: 93 3506
BsiEI CG_ry'CG
Cuts at: 104 1141 1187 2395 104
Size: 1037 46 1208 1308
Fragments arranged by size:
1308 1208 1037 46
BsiHKAI G_wGCw'C
Cuts at: 613 1391 1581 2373 613
Size: 778 190 792 1839
Fragments arranged by size:
1839 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 112 112
Size: 3599
Bsp1286I G_dGCh'C
Cuts at: 613 1224 1317 1391 1581 1643 2373 613
Size: 611 93 74 190 62 730 1839
Fragments arranged by size:
1839 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2819 2819
Size: 3599
BspHI T'CATG_A
Cuts at: 2775 2775
Size: 3599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 478 649 863 1165 1546 478
Size: 171 214 302 381 2531
Fragments arranged by size:
2531 381 302 214 171
BsrBI CCG'CTC
Cuts at: 50 1891 1945 3429 3594 50
Size: 1841 54 1484 165 55
Fragments arranged by size:
1841 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 401 527 1511 401
Size: 126 984 2489
Fragments arranged by size:
2489 984 126
BsrFI r'CCGG_y
Cuts at: 236 285 1597 1778 1987 236
Size: 49 1312 181 209 1848
Fragments arranged by size:
1848 1312 209 181 49
BsrGI T'GTAC_A
Cuts at: 359 359
Size: 3599
BssSI C'ACGA_G
Cuts at: 1870 2228 1870
Size: 358 3241
BstAPI GCAn_nnn'nTGC
Cuts at: 976 976
Size: 3599
BstBI TT'CG_AA
Cuts at: 1960 1960
Size: 3599
BstXI CCAn_nnnn'nTGG
Cuts at: 114 393 114
Size: 279 3320
BstYI r'GATC_y
Cuts at: 1 228 317 624 1091 1114 1449 1695 2707
Size: 227 89 307 467 23 335 246 1012
Cuts at: 2707 2793 2805 3151 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 307 246 227
89 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3599
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1549 1730 1893 1549
Size: 181 163 3255
Fragments arranged by size:
3255 181 163
BtsI GCAGTG_nn'
Cuts at: 842 842
Size: 3599
DraI TTT'AAA
Cuts at: 598 598
Size: 3599
DrdI GACnn_nn'nnGTC
Cuts at: 1305 2163 1305
Size: 858 2741
EaeI y'GGCC_r
Cuts at: 383 1184 1358 1749 1776 3121 383
Size: 801 174 391 27 1345 861
Fragments arranged by size:
1345 861 801 391 174 27
EagI C'GGCC_G
Cuts at: 1184 1184
Size: 3599
EarI CTCTTCn'nnn_
Cuts at: 148 502 570 1622 1832 148
Size: 354 68 1052 210 1915
Fragments arranged by size:
1915 1052 354 210 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 1807 2117 2263 3409 145
Size: 1662 310 146 1146 335
Fragments arranged by size:
1662 1146 335 310 146
EcoICRI GAG'CTC
Cuts at: 611 611
Size: 3599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 473 1424 1856 2603 2871 136
Size: 337 951 432 747 268 864
Fragments arranged by size:
951 864 747 432 337 268
EcoO109I rG'GnC_Cy
Cuts at: 738 738
Size: 3599
EcoRI G'AATT_C
Cuts at: 410 603 410
Size: 193 3406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 279 1618 279
Size: 1339 2260
FspI TGC'GCA
Cuts at: 1380 1380
Size: 3599
HaeII r_GCGC'y
Cuts at: 1033 1281 2303 3282 1033
Size: 248 1022 979 1350
Fragments arranged by size:
1350 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 1441 1471 1473 1503 142
Size: 32 1267 30 2 30 2238
Fragments arranged by size:
2238 1267 32 30 30 2
HincII GTy'rAC
Cuts at: 638 638
Size: 3599
KasI G'GCGC_C
Cuts at: 1029 1277 1029
Size: 248 3351
KpnI G_GTAC'C
Cuts at: 619 619
Size: 3599
MluI A'CGCG_T
Cuts at: 3032 3032
Size: 3599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2270 2454 2270
Size: 184 3415
MscI TGG'CCA
Cuts at: 385 1360 385
Size: 975 2624
MslI CAynn'nnrTG
Cuts at: 1588 1715 1588
Size: 127 3472
MspA1I CmG'CkG
Cuts at: 176 431 710 1024 1384 2397 2642 176
Size: 255 279 314 360 1013 245 1133
Fragments arranged by size:
1133 1013 360 314 279 255 245
NaeI GCC'GGC
Cuts at: 1780 1780
Size: 3599
NarI GG'CG_CC
Cuts at: 1030 1278 1030
Size: 248 3351
NcoI C'CATG_G
Cuts at: 107 107
Size: 3599
NgoMIV G'CCGG_C
Cuts at: 1778 1778
Size: 3599
NspI r_CATG'y
Cuts at: 652 1152 2059 652
Size: 500 907 2192
Fragments arranged by size:
2192 907 500
PciI A'CATG_T
Cuts at: 2055 2055
Size: 3599
PmeI GTTT'AAAC
Cuts at: 598 598
Size: 3599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2770 2738
Size: 32 3567
PspXI vC'TCGA_Gb
Cuts at: 181 181
Size: 3599
PstI C_TGCA'G
Cuts at: 325 646 1331 325
Size: 321 685 2593
Fragments arranged by size:
2593 685 321
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3599
PvuII CAG'CTG
Cuts at: 431 1024 1384 431
Size: 593 360 2646
Fragments arranged by size:
2646 593 360
RsrII CG'GwC_CG
Cuts at: 1794 1794
Size: 3599
SacI G_AGCT'C
Cuts at: 613 613
Size: 3599
SalI G'TCGA_C
Cuts at: 636 636
Size: 3599
SapI GCTCTTCn'nnn_
Cuts at: 148 1622 1832 148
Size: 1474 210 1915
Fragments arranged by size:
1915 1474 210
SbfI CC_TGCA'GG
Cuts at: 646 646
Size: 3599
ScaI AGT'ACT
Cuts at: 3116 3116
Size: 3599
SfcI C'TryA_G
Cuts at: 33 162 321 642 1327 2320 2511 33
Size: 129 159 321 685 993 191 1121
Fragments arranged by size:
1121 993 685 321 191 159 129
SfoI GGC'GCC
Cuts at: 1031 1279 1031
Size: 248 3351
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3599
SmaI CCC'GGG
Cuts at: 621 621
Size: 3599
SmlI C'TyrA_G
Cuts at: 181 253 754 1669 2161 2423 181
Size: 72 501 915 492 262 1357
Fragments arranged by size:
1357 915 501 492 262 72
SnaBI TAC'GTA
Cuts at: 354 354
Size: 3599
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3599
SphI G_CATG'C
Cuts at: 652 1152 652
Size: 500 3099
StyI C'CwwG_G
Cuts at: 107 733 1087 107
Size: 626 354 2619
Fragments arranged by size:
2619 626 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1182 1315 1774 1983 1182
Size: 133 459 209 2798
Fragments arranged by size:
2798 459 209 133
TatI w'GTAC_w
Cuts at: 359 3114 359
Size: 2755 844
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 3567
Tth111I GACn'n_nGTC
Cuts at: 1396 1396
Size: 3599
XbaI T'CTAG_A
Cuts at: 630 630
Size: 3599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1094 1094
Size: 3599
XhoI C'TCGA_G
Cuts at: 181 181
Size: 3599
XmaI C'CCGG_G
Cuts at: 619 619
Size: 3599
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BseRI BsiWI BsmI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XmnI ZraI