Restriction map for
pF1KE0218
(Circular) (Six-base) MAPSORT of: pF1KE0218.seq Check: 9304 from: 1 to: 3647
pF1KE0218 3647 bp
With 182 enzymes: SgfI *
October 6, 2008 10:37 ..
AccI GT'mk_AC
Cuts at: 128 685 128
Size: 557 3090
Acc65I G'GTAC_C
Cuts at: 663 663
Size: 3647
AclI AA'CG_TT
Cuts at: 3487 3487
Size: 3647
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1472 1904 2651 2919 1472
Size: 432 747 268 2200
Fragments arranged by size:
2200 747 432 268
AflIII A'CryG_T
Cuts at: 999 2103 3080 999
Size: 1104 977 1566
Fragments arranged by size:
1566 1104 977
AgeI A'CCGG_T
Cuts at: 2035 2035
Size: 3647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 643 643
Size: 3647
AlwNI CAG_nnn'CTG
Cuts at: 952 2519 952
Size: 1567 2080
ApaLI G'TGCA_C
Cuts at: 2417 2417
Size: 3647
ApoI r'AATT_y
Cuts at: 651 3000 3256 651
Size: 2349 256 1042
Fragments arranged by size:
2349 1042 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3647
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3647
AvaI C'yCGr_G
Cuts at: 667 667
Size: 3647
BamHI G'GATC_C
Cuts at: 672 672
Size: 3647
BanI G'GyrC_C
Cuts at: 663 1077 1325 1360 663
Size: 414 248 35 2950
Fragments arranged by size:
2950 414 248 35
BanII G_rGCy'C
Cuts at: 661 1691 661
Size: 1030 2617
BbeI G_GCGC'C
Cuts at: 1081 1329 1081
Size: 248 3399
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 444 478 444
Size: 34 3613
BciVI GTATCCnnnnn_n'
Cuts at: 1545 2306 3647 1545
Size: 761 1341 1545
Fragments arranged by size:
1545 1341 761
BclI T'GATC_A
Cuts at: 1167 1167
Size: 3647
BfrBI ATG'CAT
Cuts at: 506 506
Size: 3647
BglII A'GATC_T
Cuts at: 1162 3199 1162
Size: 2037 1610
BlpI GC'TnA_GC
Cuts at: 214 759 214
Size: 545 3102
Bme1580I G_kGCm'C
Cuts at: 1272 1365 2421 1272
Size: 93 1056 2498
Fragments arranged by size:
2498 1056 93
BmgBI CAC'GTC
Cuts at: 294 294
Size: 3647
BmrI ACTGGGnnnn_n'
Cuts at: 859 940 1275 3419 859
Size: 81 335 2144 1087
Fragments arranged by size:
2144 1087 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2014 2014
Size: 3647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 1702 2194 2492 823
Size: 879 492 298 1978
Fragments arranged by size:
1978 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3351 3351
Size: 3647
BsaAI yAC'GTr
Cuts at: 126 1000 126
Size: 874 2773
BsaBI GATnn'nnATC
Cuts at: 6 1161 3198 6
Size: 1155 2037 455
Fragments arranged by size:
2037 1155 455
BsaHI Gr'CG_yC
Cuts at: 1078 1326 2028 3524 1078
Size: 248 702 1496 1201
Fragments arranged by size:
1496 1201 702 248
BsaWI w'CCGG_w
Cuts at: 836 1357 2035 2309 2456 2867 836
Size: 521 678 274 147 411 1616
Fragments arranged by size:
1616 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1072 1830 2407 2903 1072
Size: 758 577 496 1816
Fragments arranged by size:
1816 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 466
Size: 3647
BsiEI CG_ry'CG
Cuts at: 104 1189 1235 2443 104
Size: 1085 46 1208 1308
Fragments arranged by size:
1308 1208 1085 46
BsiHKAI G_wGCw'C
Cuts at: 661 1439 1629 2421 661
Size: 778 190 792 1887
Fragments arranged by size:
1887 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 661 1272 1365 1439 1629 1691 2421 661
Size: 611 93 74 190 62 730 1887
Fragments arranged by size:
1887 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2867 2867
Size: 3647
BspHI T'CATG_A
Cuts at: 2823 2823
Size: 3647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 697 911 1213 1594 697
Size: 214 302 381 2750
Fragments arranged by size:
2750 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1939 1993 3477 3642 50
Size: 1889 54 1484 165 55
Fragments arranged by size:
1889 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 618 1559 618
Size: 941 2706
BsrFI r'CCGG_y
Cuts at: 1645 1826 2035 1645
Size: 181 209 3257
Fragments arranged by size:
3257 209 181
BssSI C'ACGA_G
Cuts at: 1918 2276 1918
Size: 358 3289
BstAPI GCAn_nnn'nTGC
Cuts at: 1024 1024
Size: 3647
BstBI TT'CG_AA
Cuts at: 2008 2008
Size: 3647
BstYI r'GATC_y
Cuts at: 1 672 1139 1162 1497 1743 2755 2841 2853
Size: 671 467 23 335 246 1012 86 12
Cuts at: 2853 3199 1
Size: 346 449
Fragments arranged by size:
1012 671 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 200 200
Size: 3647
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1597 1778 1941 1597
Size: 181 163 3303
Fragments arranged by size:
3303 181 163
BtsI GCAGTG_nn'
Cuts at: 890 890
Size: 3647
DraI TTT'AAA
Cuts at: 646 646
Size: 3647
DrdI GACnn_nn'nnGTC
Cuts at: 1353 2211 1353
Size: 858 2789
EaeI y'GGCC_r
Cuts at: 1232 1406 1797 1824 3169 1232
Size: 174 391 27 1345 1710
Fragments arranged by size:
1710 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1232 1232
Size: 3647
EarI CTCTTCn'nnn_
Cuts at: 1670 1880 1670
Size: 210 3437
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1855 2165 2311 3457 1855
Size: 310 146 1146 2045
Fragments arranged by size:
2045 1146 310 146
EcoICRI GAG'CTC
Cuts at: 659 659
Size: 3647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1472 1904 2651 2919 1472
Size: 432 747 268 2200
Fragments arranged by size:
2200 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 786 786
Size: 3647
EcoRI G'AATT_C
Cuts at: 651 651
Size: 3647
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 324 387 1666 324
Size: 63 1279 2305
Fragments arranged by size:
2305 1279 63
FspI TGC'GCA
Cuts at: 1428 1428
Size: 3647
HaeII r_GCGC'y
Cuts at: 1081 1329 2351 3330 1081
Size: 248 1022 979 1398
Fragments arranged by size:
1398 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1489 1519 1521 1551 1489
Size: 30 2 30 3585
Fragments arranged by size:
3585 30 30 2
HincII GTy'rAC
Cuts at: 264 686 264
Size: 422 3225
KasI G'GCGC_C
Cuts at: 1077 1325 1077
Size: 248 3399
KpnI G_GTAC'C
Cuts at: 667 667
Size: 3647
MluI A'CGCG_T
Cuts at: 3080 3080
Size: 3647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2318 2502 2318
Size: 184 3463
MscI TGG'CCA
Cuts at: 1408 1408
Size: 3647
MslI CAynn'nnrTG
Cuts at: 436 1636 1763 436
Size: 1200 127 2320
Fragments arranged by size:
2320 1200 127
MspA1I CmG'CkG
Cuts at: 758 1072 1432 2445 2690 758
Size: 314 360 1013 245 1715
Fragments arranged by size:
1715 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1828 1828
Size: 3647
NarI GG'CG_CC
Cuts at: 1078 1326 1078
Size: 248 3399
NcoI C'CATG_G
Cuts at: 200 200
Size: 3647
NgoMIV G'CCGG_C
Cuts at: 1826 1826
Size: 3647
NsiI A_TGCA'T
Cuts at: 508 508
Size: 3647
NspI r_CATG'y
Cuts at: 700 1200 2107 700
Size: 500 907 2240
Fragments arranged by size:
2240 907 500
PciI A'CATG_T
Cuts at: 2103 2103
Size: 3647
PfoI T'CCnGG_A
Cuts at: 161 161
Size: 3647
PmeI GTTT'AAAC
Cuts at: 646 646
Size: 3647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2786 2818 2786
Size: 32 3615
PstI C_TGCA'G
Cuts at: 694 1379 694
Size: 685 2962
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3647
PvuII CAG'CTG
Cuts at: 1072 1432 1072
Size: 360 3287
RsrII CG'GwC_CG
Cuts at: 1842 1842
Size: 3647
SacI G_AGCT'C
Cuts at: 661 661
Size: 3647
SalI G'TCGA_C
Cuts at: 684 684
Size: 3647
SapI GCTCTTCn'nnn_
Cuts at: 1670 1880 1670
Size: 210 3437
SbfI CC_TGCA'GG
Cuts at: 694 694
Size: 3647
ScaI AGT'ACT
Cuts at: 3164 3164
Size: 3647
SexAI A'CCwGG_T
Cuts at: 257 257
Size: 3647
SfcI C'TryA_G
Cuts at: 33 690 1375 2368 2559 33
Size: 657 685 993 191 1121
Fragments arranged by size:
1121 993 685 657 191
SfoI GGC'GCC
Cuts at: 1079 1327 1079
Size: 248 3399
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3647
SmaI CCC'GGG
Cuts at: 669 669
Size: 3647
SmlI C'TyrA_G
Cuts at: 802 1717 2209 2471 802
Size: 915 492 262 1978
Fragments arranged by size:
1978 915 492 262
SnaBI TAC'GTA
Cuts at: 126 126
Size: 3647
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3647
SphI G_CATG'C
Cuts at: 700 1200 700
Size: 500 3147
SspI AAT'ATT
Cuts at: 605 605
Size: 3647
StyI C'CwwG_G
Cuts at: 200 245 781 1135 200
Size: 45 536 354 2712
Fragments arranged by size:
2712 536 354 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1230 1363 1822 2031 1230
Size: 133 459 209 2846
Fragments arranged by size:
2846 459 209 133
TatI w'GTAC_w
Cuts at: 3162 3162
Size: 3647
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3615
Tth111I GACn'n_nGTC
Cuts at: 259 295 1444 259
Size: 36 1149 2462
Fragments arranged by size:
2462 1149 36
XbaI T'CTAG_A
Cuts at: 678 678
Size: 3647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1142 1142
Size: 3647
XmaI C'CCGG_G
Cuts at: 667 667
Size: 3647
XmnI GAAnn'nnTTC
Cuts at: 604 604
Size: 3647
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BglI BmtI BpmI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SrfI StuI SwaI XhoI ZraI