Restriction map for
pF1KE0215
(Circular) (Six-base) MAPSORT of: pF1KE0215.seq Check: 7993 from: 1 to: 3446
pF1KE0215 3446 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 431 484 431
Size: 53 3393
Acc65I G'GTAC_C
Cuts at: 338 462 338
Size: 124 3322
AclI AA'CG_TT
Cuts at: 3286 3286
Size: 3446
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1271 1703 2450 2718 1271
Size: 432 747 268 1999
Fragments arranged by size:
1999 747 432 268
AfeI AGC'GCT
Cuts at: 388 388
Size: 3446
AflIII A'CryG_T
Cuts at: 798 1902 2879 798
Size: 1104 977 1365
Fragments arranged by size:
1365 1104 977
AgeI A'CCGG_T
Cuts at: 1834 1834
Size: 3446
AlwNI CAG_nnn'CTG
Cuts at: 751 2318 751
Size: 1567 1879
ApaLI G'TGCA_C
Cuts at: 2216 2216
Size: 3446
ApoI r'AATT_y
Cuts at: 450 2799 3055 450
Size: 2349 256 841
Fragments arranged by size:
2349 841 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3446
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3446
AvaI C'yCGr_G
Cuts at: 466 466
Size: 3446
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 330 363 330
Size: 33 3413
BamHI G'GATC_C
Cuts at: 471 471
Size: 3446
BanI G'GyrC_C
Cuts at: 338 462 876 1124 1159 338
Size: 124 414 248 35 2625
Fragments arranged by size:
2625 414 248 124 35
BanII G_rGCy'C
Cuts at: 460 1490 460
Size: 1030 2416
BbeI G_GCGC'C
Cuts at: 880 1128 880
Size: 248 3198
BciVI GTATCCnnnnn_n'
Cuts at: 1344 2105 3446 1344
Size: 761 1341 1344
Fragments arranged by size:
1344 1341 761
BclI T'GATC_A
Cuts at: 966 966
Size: 3446
BglII A'GATC_T
Cuts at: 961 2998 961
Size: 2037 1409
BlpI GC'TnA_GC
Cuts at: 558 558
Size: 3446
Bme1580I G_kGCm'C
Cuts at: 1071 1164 2220 1071
Size: 93 1056 2297
Fragments arranged by size:
2297 1056 93
BmgBI CAC'GTC
Cuts at: 258 258
Size: 3446
BmrI ACTGGGnnnn_n'
Cuts at: 658 739 1074 3218 658
Size: 81 335 2144 886
Fragments arranged by size:
2144 886 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1813 1813
Size: 3446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 1501 1993 2291 622
Size: 879 492 298 1777
Fragments arranged by size:
1777 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3150 3150
Size: 3446
BsaAI yAC'GTr
Cuts at: 799 799
Size: 3446
BsaBI GATnn'nnATC
Cuts at: 6 310 960 2997 6
Size: 304 650 2037 455
Fragments arranged by size:
2037 650 455 304
BsaHI Gr'CG_yC
Cuts at: 877 1125 1827 3323 877
Size: 248 702 1496 1000
Fragments arranged by size:
1496 1000 702 248
BsaWI w'CCGG_w
Cuts at: 135 635 1156 1834 2108 2255 2666 135
Size: 500 521 678 274 147 411 915
Fragments arranged by size:
915 678 521 500 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 313
Size: 3446
BseYI C'CCAG_C
Cuts at: 871 1629 2206 2702 871
Size: 758 577 496 1615
Fragments arranged by size:
1615 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 346
Size: 3446
BsiEI CG_ry'CG
Cuts at: 104 161 988 1034 2242 104
Size: 57 827 46 1208 1308
Fragments arranged by size:
1308 1208 827 57 46
BsiHKAI G_wGCw'C
Cuts at: 226 356 382 460 1238 1428 2220 226
Size: 130 26 78 778 190 792 1452
Fragments arranged by size:
1452 792 778 190 130 78 26
Bsp1286I G_dGCh'C
Cuts at: 226 356 382 460 1071 1164 1238 1428 1490
Size: 130 26 78 611 93 74 190 62
Cuts at: 1490 2220 226
Size: 730 1452
Fragments arranged by size:
1452 730 611 190 130 93 78 74
62 26
BspEI T'CCGG_A
Cuts at: 2666 2666
Size: 3446
BspHI T'CATG_A
Cuts at: 2622 2622
Size: 3446
BspMI ACCTGCnnnn'nnnn_
Cuts at: 496 710 1012 1393 496
Size: 214 302 381 2549
Fragments arranged by size:
2549 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1738 1792 3276 3441 50
Size: 1688 54 1484 165 55
Fragments arranged by size:
1688 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1358 1358
Size: 3446
BsrFI r'CCGG_y
Cuts at: 1444 1625 1834 1444
Size: 181 209 3056
Fragments arranged by size:
3056 209 181
BsrGI T'GTAC_A
Cuts at: 188 188
Size: 3446
BssSI C'ACGA_G
Cuts at: 1717 2075 1717
Size: 358 3088
BstAPI GCAn_nnn'nTGC
Cuts at: 360 823 360
Size: 463 2983
BstBI TT'CG_AA
Cuts at: 1807 1807
Size: 3446
BstYI r'GATC_y
Cuts at: 1 242 471 938 961 1296 1542 2554 2640
Size: 241 229 467 23 335 246 1012 86
Cuts at: 2640 2652 2998 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 246 241 229
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1396 1577 1740 1396
Size: 181 163 3102
Fragments arranged by size:
3102 181 163
BtsI GCAGTG_nn'
Cuts at: 291 354 689 291
Size: 63 335 3048
Fragments arranged by size:
3048 335 63
DraI TTT'AAA
Cuts at: 445 445
Size: 3446
DrdI GACnn_nn'nnGTC
Cuts at: 1152 2010 1152
Size: 858 2588
EaeI y'GGCC_r
Cuts at: 158 1031 1205 1596 1623 2968 158
Size: 873 174 391 27 1345 636
Fragments arranged by size:
1345 873 636 391 174 27
EagI C'GGCC_G
Cuts at: 158 1031 158
Size: 873 2573
EarI CTCTTCn'nnn_
Cuts at: 1469 1679 1469
Size: 210 3236
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1654 1964 2110 3256 1654
Size: 310 146 1146 1844
Fragments arranged by size:
1844 1146 310 146
EcoICRI GAG'CTC
Cuts at: 458 458
Size: 3446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1271 1703 2450 2718 1271
Size: 432 747 268 1999
Fragments arranged by size:
1999 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 235 585 235
Size: 350 3096
EcoRI G'AATT_C
Cuts at: 450 450
Size: 3446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1465 1465
Size: 3446
FspI TGC'GCA
Cuts at: 1227 1227
Size: 3446
HaeII r_GCGC'y
Cuts at: 187 390 880 1128 2150 3129 187
Size: 203 490 248 1022 979 504
Fragments arranged by size:
1022 979 504 490 248 203
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 227 259 1288 1318 1320 1350 227
Size: 32 1029 30 2 30 2323
Fragments arranged by size:
2323 1029 32 30 30 2
HincII GTy'rAC
Cuts at: 485 485
Size: 3446
KasI G'GCGC_C
Cuts at: 876 1124 876
Size: 248 3198
KpnI G_GTAC'C
Cuts at: 342 466 342
Size: 124 3322
MluI A'CGCG_T
Cuts at: 2879 2879
Size: 3446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2117 2301 2117
Size: 184 3262
MscI TGG'CCA
Cuts at: 1207 1207
Size: 3446
MslI CAynn'nnrTG
Cuts at: 1435 1562 1435
Size: 127 3319
MspA1I CmG'CkG
Cuts at: 557 871 1231 2244 2489 557
Size: 314 360 1013 245 1514
Fragments arranged by size:
1514 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1627 1627
Size: 3446
NarI GG'CG_CC
Cuts at: 877 1125 877
Size: 248 3198
NgoMIV G'CCGG_C
Cuts at: 1625 1625
Size: 3446
NotI GC'GGCC_GC
Cuts at: 158 158
Size: 3446
NspI r_CATG'y
Cuts at: 499 999 1906 499
Size: 500 907 2039
Fragments arranged by size:
2039 907 500
PciI A'CATG_T
Cuts at: 1902 1902
Size: 3446
PfoI T'CCnGG_A
Cuts at: 226 226
Size: 3446
PmeI GTTT'AAAC
Cuts at: 445 445
Size: 3446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2585 2617 2585
Size: 32 3414
PpuMI rG'GwC_Cy
Cuts at: 235 235
Size: 3446
PstI C_TGCA'G
Cuts at: 377 493 1178 377
Size: 116 685 2645
Fragments arranged by size:
2645 685 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3446
PvuII CAG'CTG
Cuts at: 871 1231 871
Size: 360 3086
RsrII CG'GwC_CG
Cuts at: 1641 1641
Size: 3446
SacI G_AGCT'C
Cuts at: 460 460
Size: 3446
SalI G'TCGA_C
Cuts at: 483 483
Size: 3446
SanDI GG'GwC_CC
Cuts at: 235 235
Size: 3446
SapI GCTCTTCn'nnn_
Cuts at: 1469 1679 1469
Size: 210 3236
SbfI CC_TGCA'GG
Cuts at: 493 493
Size: 3446
ScaI AGT'ACT
Cuts at: 2963 2963
Size: 3446
SfcI C'TryA_G
Cuts at: 33 249 373 489 1174 2167 2358 33
Size: 216 124 116 685 993 191 1121
Fragments arranged by size:
1121 993 685 216 191 124 116
SfoI GGC'GCC
Cuts at: 878 1126 878
Size: 248 3198
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3446
SmaI CCC'GGG
Cuts at: 468 468
Size: 3446
SmlI C'TyrA_G
Cuts at: 601 1516 2008 2270 601
Size: 915 492 262 1777
Fragments arranged by size:
1777 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3446
SphI G_CATG'C
Cuts at: 499 999 499
Size: 500 2946
StyI C'CwwG_G
Cuts at: 580 934 580
Size: 354 3092
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1029 1162 1621 1830 1029
Size: 133 459 209 2645
Fragments arranged by size:
2645 459 209 133
TatI w'GTAC_w
Cuts at: 188 2961 188
Size: 2773 673
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 3414
Tth111I GACn'n_nGTC
Cuts at: 1243 1243
Size: 3446
XbaI T'CTAG_A
Cuts at: 477 477
Size: 3446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 941 941
Size: 3446
XmaI C'CCGG_G
Cuts at: 466 466
Size: 3446
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NotI NspI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BcgI BfrBI BglI BmtI
BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI