Restriction map for
pF1KE0204
(Circular) (Six-base) MAPSORT of: pF1KE0204.seq Check: 2246 from: 1 to: 3734
pF1KE0204 3734 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 772 772
Size: 3734
Acc65I G'GTAC_C
Cuts at: 750 750
Size: 3734
AclI AA'CG_TT
Cuts at: 3574 3574
Size: 3734
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1559 1991 2738 3006 646
Size: 913 432 747 268 1374
Fragments arranged by size:
1374 913 747 432 268
AfeI AGC'GCT
Cuts at: 637 637
Size: 3734
AflIII A'CryG_T
Cuts at: 1086 2190 3167 1086
Size: 1104 977 1653
Fragments arranged by size:
1653 1104 977
AgeI A'CCGG_T
Cuts at: 2122 2122
Size: 3734
AleI CACnn'nnGTG
Cuts at: 685 685
Size: 3734
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 475 507 475
Size: 32 3702
AlwNI CAG_nnn'CTG
Cuts at: 180 312 1039 2606 180
Size: 132 727 1567 1308
Fragments arranged by size:
1567 1308 727 132
ApaI G_GGCC'C
Cuts at: 595 595
Size: 3734
ApaLI G'TGCA_C
Cuts at: 2504 2504
Size: 3734
ApoI r'AATT_y
Cuts at: 421 738 3087 3343 421
Size: 317 2349 256 812
Fragments arranged by size:
2349 812 317 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
AvaI C'yCGr_G
Cuts at: 236 602 754 236
Size: 366 152 3216
Fragments arranged by size:
3216 366 152
BamHI G'GATC_C
Cuts at: 707 759 707
Size: 52 3682
BanI G'GyrC_C
Cuts at: 472 750 1164 1412 1447 472
Size: 278 414 248 35 2759
Fragments arranged by size:
2759 414 278 248 35
BanII G_rGCy'C
Cuts at: 237 595 748 1778 237
Size: 358 153 1030 2193
Fragments arranged by size:
2193 1030 358 153
BbeI G_GCGC'C
Cuts at: 1168 1416 1168
Size: 248 3486
BciVI GTATCCnnnnn_n'
Cuts at: 1632 2393 3734 1632
Size: 761 1341 1632
Fragments arranged by size:
1632 1341 761
BclI T'GATC_A
Cuts at: 1254 1254
Size: 3734
BglII A'GATC_T
Cuts at: 1249 3286 1249
Size: 2037 1697
BlpI GC'TnA_GC
Cuts at: 846 846
Size: 3734
Bme1580I G_kGCm'C
Cuts at: 333 595 1359 1452 2508 333
Size: 262 764 93 1056 1559
Fragments arranged by size:
1559 1056 764 262 93
BmgBI CAC'GTC
Cuts at: 621 621
Size: 3734
BmrI ACTGGGnnnn_n'
Cuts at: 946 1027 1362 3506 946
Size: 81 335 2144 1174
Fragments arranged by size:
2144 1174 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 3734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 529
Size: 3734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1789 2281 2579 910
Size: 879 492 298 2065
Fragments arranged by size:
2065 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3438 3438
Size: 3734
BsaAI yAC'GTr
Cuts at: 216 558 1087 216
Size: 342 529 2863
Fragments arranged by size:
2863 529 342
BsaBI GATnn'nnATC
Cuts at: 6 1248 3285 6
Size: 1242 2037 455
Fragments arranged by size:
2037 1242 455
BsaHI Gr'CG_yC
Cuts at: 1165 1413 2115 3611 1165
Size: 248 702 1496 1288
Fragments arranged by size:
1496 1288 702 248
BsaWI w'CCGG_w
Cuts at: 398 923 1444 2122 2396 2543 2954 398
Size: 525 521 678 274 147 411 1178
Fragments arranged by size:
1178 678 525 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 475 505 475
Size: 30 3704
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 205
Size: 3734
BseYI C'CCAG_C
Cuts at: 359 1159 1917 2494 2990 359
Size: 800 758 577 496 1103
Fragments arranged by size:
1103 800 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 114
Size: 3734
BsiEI CG_ry'CG
Cuts at: 104 583 1276 1322 2530 104
Size: 479 693 46 1208 1308
Fragments arranged by size:
1308 1208 693 479 46
BsiHKAI G_wGCw'C
Cuts at: 748 1526 1716 2508 748
Size: 778 190 792 1974
Fragments arranged by size:
1974 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 409 409
Size: 3734
Bsp1286I G_dGCh'C
Cuts at: 237 333 595 748 1359 1452 1526 1716 1778
Size: 96 262 153 611 93 74 190 62
Cuts at: 1778 2508 237
Size: 730 1463
Fragments arranged by size:
1463 730 611 262 190 153 96 93
74 62
BspEI T'CCGG_A
Cuts at: 398 2954 398
Size: 2556 1178
BspHI T'CATG_A
Cuts at: 629 2910 629
Size: 2281 1453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 784 998 1300 1681 784
Size: 214 302 381 2837
Fragments arranged by size:
2837 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2026 2080 3564 3729 50
Size: 1976 54 1484 165 55
Fragments arranged by size:
1976 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1646 1646
Size: 3734
BsrFI r'CCGG_y
Cuts at: 1732 1913 2122 1732
Size: 181 209 3344
Fragments arranged by size:
3344 209 181
BssSI C'ACGA_G
Cuts at: 454 2005 2363 454
Size: 1551 358 1825
Fragments arranged by size:
1825 1551 358
BstAPI GCAn_nnn'nTGC
Cuts at: 621 1111 621
Size: 490 3244
BstBI TT'CG_AA
Cuts at: 2095 2095
Size: 3734
BstEII G'GTnAC_C
Cuts at: 687 687
Size: 3734
BstYI r'GATC_y
Cuts at: 1 707 759 1226 1249 1584 1830 2842 2928
Size: 706 52 467 23 335 246 1012 86
Cuts at: 2928 2940 3286 1
Size: 12 346 449
Fragments arranged by size:
1012 706 467 449 346 335 246 86
52 23 12
BtgI C'CryG_G
Cuts at: 333 583 333
Size: 250 3484
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1684 1865 2028 1684
Size: 181 163 3390
Fragments arranged by size:
3390 181 163
BtsI GCAGTG_nn'
Cuts at: 977 977
Size: 3734
DraI TTT'AAA
Cuts at: 733 733
Size: 3734
DrdI GACnn_nn'nnGTC
Cuts at: 696 1440 2298 696
Size: 744 858 2132
Fragments arranged by size:
2132 858 744
EaeI y'GGCC_r
Cuts at: 580 1319 1493 1884 1911 3256 580
Size: 739 174 391 27 1345 1058
Fragments arranged by size:
1345 1058 739 391 174 27
EagI C'GGCC_G
Cuts at: 580 1319 580
Size: 739 2995
EarI CTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 114 1942 2252 2398 3544 114
Size: 1828 310 146 1146 304
Fragments arranged by size:
1828 1146 310 304 146
EcoICRI GAG'CTC
Cuts at: 746 746
Size: 3734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 646 1559 1991 2738 3006 529
Size: 117 913 432 747 268 1257
Fragments arranged by size:
1257 913 747 432 268 117
EcoO109I rG'GnC_Cy
Cuts at: 309 591 592 698 873 309
Size: 282 1 106 175 3170
Fragments arranged by size:
3170 282 175 106 1
EcoRI G'AATT_C
Cuts at: 421 738 421
Size: 317 3417
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1753 1753
Size: 3734
FspI TGC'GCA
Cuts at: 1515 1515
Size: 3734
HaeII r_GCGC'y
Cuts at: 121 639 1168 1416 2438 3417 121
Size: 518 529 248 1022 979 438
Fragments arranged by size:
1022 979 529 518 438 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 219 251 687 719 1576 1606 1608 1638 219
Size: 32 436 32 857 30 2 30 2315
Fragments arranged by size:
2315 857 436 32 32 30 30 2
HincII GTy'rAC
Cuts at: 773 773
Size: 3734
KasI G'GCGC_C
Cuts at: 1164 1412 1164
Size: 248 3486
KpnI G_GTAC'C
Cuts at: 754 754
Size: 3734
MluI A'CGCG_T
Cuts at: 3167 3167
Size: 3734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 685 2405 2589 685
Size: 1720 184 1830
Fragments arranged by size:
1830 1720 184
MscI TGG'CCA
Cuts at: 1495 1495
Size: 3734
MslI CAynn'nnrTG
Cuts at: 214 685 1723 1850 214
Size: 471 1038 127 2098
Fragments arranged by size:
2098 1038 471 127
MspA1I CmG'CkG
Cuts at: 261 335 585 845 1159 1519 2532 2777 261
Size: 74 250 260 314 360 1013 245 1218
Fragments arranged by size:
1218 1013 360 314 260 250 245 74
NaeI GCC'GGC
Cuts at: 1915 1915
Size: 3734
NarI GG'CG_CC
Cuts at: 1165 1413 1165
Size: 248 3486
NgoMIV G'CCGG_C
Cuts at: 1913 1913
Size: 3734
NotI GC'GGCC_GC
Cuts at: 580 580
Size: 3734
NspI r_CATG'y
Cuts at: 787 1287 2194 787
Size: 500 907 2327
Fragments arranged by size:
2327 907 500
PciI A'CATG_T
Cuts at: 2190 2190
Size: 3734
PflMI CCAn_nnn'nTGG
Cuts at: 312 312
Size: 3734
PmeI GTTT'AAAC
Cuts at: 733 733
Size: 3734
PmlI CAC'GTG
Cuts at: 216 216
Size: 3734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 475 507 2873 2905 475
Size: 32 2366 32 1304
Fragments arranged by size:
2366 1304 32 32
PpuMI rG'GwC_Cy
Cuts at: 309 698 309
Size: 389 3345
PspOMI G'GGCC_C
Cuts at: 591 591
Size: 3734
PstI C_TGCA'G
Cuts at: 781 1466 781
Size: 685 3049
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3734
PvuII CAG'CTG
Cuts at: 261 1159 1519 261
Size: 898 360 2476
Fragments arranged by size:
2476 898 360
RsrII CG'GwC_CG
Cuts at: 1929 1929
Size: 3734
SacI G_AGCT'C
Cuts at: 748 748
Size: 3734
SacII CC_GC'GG
Cuts at: 336 586 336
Size: 250 3484
SalI G'TCGA_C
Cuts at: 771 771
Size: 3734
SapI GCTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
SbfI CC_TGCA'GG
Cuts at: 781 781
Size: 3734
ScaI AGT'ACT
Cuts at: 544 3251 544
Size: 2707 1027
SfcI C'TryA_G
Cuts at: 33 279 777 1462 2455 2646 33
Size: 246 498 685 993 191 1121
Fragments arranged by size:
1121 993 685 498 246 191
SfoI GGC'GCC
Cuts at: 1166 1414 1166
Size: 248 3486
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
SmaI CCC'GGG
Cuts at: 604 756 604
Size: 152 3582
SmlI C'TyrA_G
Cuts at: 889 1804 2296 2558 889
Size: 915 492 262 2065
Fragments arranged by size:
2065 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3734
SphI G_CATG'C
Cuts at: 787 1287 787
Size: 500 3234
StyI C'CwwG_G
Cuts at: 644 868 1222 644
Size: 224 354 3156
Fragments arranged by size:
3156 354 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1317 1450 1909 2118 1317
Size: 133 459 209 2933
Fragments arranged by size:
2933 459 209 133
TatI w'GTAC_w
Cuts at: 542 677 3249 542
Size: 135 2572 1027
Fragments arranged by size:
2572 1027 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 3702
Tth111I GACn'n_nGTC
Cuts at: 1531 1531
Size: 3734
XbaI T'CTAG_A
Cuts at: 765 765
Size: 3734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1229 1229
Size: 3734
XmaI C'CCGG_G
Cuts at: 602 754 602
Size: 152 3582
XmnI GAAnn'nnTTC
Cuts at: 670 670
Size: 3734
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NotI NspI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI
BsmI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NruI NsiI PacI PasI PfoI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI