Restriction map for
pF1KE0194
(Circular) (Six-base) MAPSORT of: pF1KE0194.seq Check: 7465 from: 1 to: 3656
pF1KE0194 3656 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 694 694
Size: 3656
Acc65I G'GTAC_C
Cuts at: 672 672
Size: 3656
AclI AA'CG_TT
Cuts at: 3496 3496
Size: 3656
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 1481 1913 2660 2928 373
Size: 1108 432 747 268 1101
Fragments arranged by size:
1108 1101 747 432 268
AflIII A'CryG_T
Cuts at: 1008 2112 3089 1008
Size: 1104 977 1575
Fragments arranged by size:
1575 1104 977
AgeI A'CCGG_T
Cuts at: 2044 2044
Size: 3656
AlwNI CAG_nnn'CTG
Cuts at: 961 2528 961
Size: 1567 2089
ApaLI G'TGCA_C
Cuts at: 2426 2426
Size: 3656
ApoI r'AATT_y
Cuts at: 179 361 452 463 660 3009 3265 179
Size: 182 91 11 197 2349 256 570
Fragments arranged by size:
2349 570 256 197 182 91 11
AseI AT'TA_AT
Cuts at: 20 20
Size: 3656
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
AvaI C'yCGr_G
Cuts at: 676 676
Size: 3656
BamHI G'GATC_C
Cuts at: 681 681
Size: 3656
BanI G'GyrC_C
Cuts at: 672 1086 1334 1369 672
Size: 414 248 35 2959
Fragments arranged by size:
2959 414 248 35
BanII G_rGCy'C
Cuts at: 670 1700 670
Size: 1030 2626
BbeI G_GCGC'C
Cuts at: 1090 1338 1090
Size: 248 3408
BciVI GTATCCnnnnn_n'
Cuts at: 1554 2315 3656 1554
Size: 761 1341 1554
Fragments arranged by size:
1554 1341 761
BclI T'GATC_A
Cuts at: 1176 1176
Size: 3656
BglI GCCn_nnn'nGGC
Cuts at: 433 433
Size: 3656
BglII A'GATC_T
Cuts at: 1171 3208 1171
Size: 2037 1619
BlpI GC'TnA_GC
Cuts at: 768 768
Size: 3656
Bme1580I G_kGCm'C
Cuts at: 1281 1374 2430 1281
Size: 93 1056 2507
Fragments arranged by size:
2507 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 868 949 1284 3428 868
Size: 81 335 2144 1096
Fragments arranged by size:
2144 1096 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2023 2023
Size: 3656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 3656
Bpu10I CC'TnA_GC
Cuts at: 550 550
Size: 3656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 832 1711 2203 2501 400
Size: 432 879 492 298 1555
Fragments arranged by size:
1555 879 492 432 298
BsaI GGTCTCn'nnnn_
Cuts at: 3360 3360
Size: 3656
BsaAI yAC'GTr
Cuts at: 1009 1009
Size: 3656
BsaBI GATnn'nnATC
Cuts at: 6 166 1170 3207 6
Size: 160 1004 2037 455
Fragments arranged by size:
2037 1004 455 160
BsaHI Gr'CG_yC
Cuts at: 1087 1335 2037 3533 1087
Size: 248 702 1496 1210
Fragments arranged by size:
1496 1210 702 248
BsaWI w'CCGG_w
Cuts at: 845 1366 2044 2318 2465 2876 845
Size: 521 678 274 147 411 1625
Fragments arranged by size:
1625 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 465 495 465
Size: 30 3626
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 175
Size: 3656
BseYI C'CCAG_C
Cuts at: 1081 1839 2416 2912 1081
Size: 758 577 496 1825
Fragments arranged by size:
1825 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1198 1244 2452 104
Size: 1094 46 1208 1308
Fragments arranged by size:
1308 1208 1094 46
BsiHKAI G_wGCw'C
Cuts at: 670 1448 1638 2430 670
Size: 778 190 792 1896
Fragments arranged by size:
1896 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 670 1281 1374 1448 1638 1700 2430 670
Size: 611 93 74 190 62 730 1896
Fragments arranged by size:
1896 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2876 2876
Size: 3656
BspHI T'CATG_A
Cuts at: 2832 2832
Size: 3656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 706 920 1222 1603 706
Size: 214 302 381 2759
Fragments arranged by size:
2759 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1948 2002 3486 3651 50
Size: 1898 54 1484 165 55
Fragments arranged by size:
1898 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1568 1568
Size: 3656
BsrFI r'CCGG_y
Cuts at: 1654 1835 2044 1654
Size: 181 209 3266
Fragments arranged by size:
3266 209 181
BssSI C'ACGA_G
Cuts at: 1927 2285 1927
Size: 358 3298
BstAPI GCAn_nnn'nTGC
Cuts at: 1033 1033
Size: 3656
BstBI TT'CG_AA
Cuts at: 2017 2017
Size: 3656
BstEII G'GTnAC_C
Cuts at: 208 208
Size: 3656
BstYI r'GATC_y
Cuts at: 1 681 1148 1171 1506 1752 2764 2850 2862
Size: 680 467 23 335 246 1012 86 12
Cuts at: 2862 3208 1
Size: 346 449
Fragments arranged by size:
1012 680 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1606 1787 1950 1606
Size: 181 163 3312
Fragments arranged by size:
3312 181 163
BtsI GCAGTG_nn'
Cuts at: 292 899 292
Size: 607 3049
DraI TTT'AAA
Cuts at: 655 655
Size: 3656
DrdI GACnn_nn'nnGTC
Cuts at: 1362 2220 1362
Size: 858 2798
EaeI y'GGCC_r
Cuts at: 1241 1415 1806 1833 3178 1241
Size: 174 391 27 1345 1719
Fragments arranged by size:
1719 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1241 1241
Size: 3656
EarI CTCTTCn'nnn_
Cuts at: 1679 1889 1679
Size: 210 3446
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1864 2174 2320 3466 1864
Size: 310 146 1146 2054
Fragments arranged by size:
2054 1146 310 146
EcoICRI GAG'CTC
Cuts at: 668 668
Size: 3656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 373 1481 1913 2660 2928 172
Size: 201 1108 432 747 268 900
Fragments arranged by size:
1108 900 747 432 268 201
EcoO109I rG'GnC_Cy
Cuts at: 279 795 279
Size: 516 3140
EcoRI G'AATT_C
Cuts at: 179 361 463 660 179
Size: 182 102 197 3175
Fragments arranged by size:
3175 197 182 102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1675 1675
Size: 3656
FspI TGC'GCA
Cuts at: 1437 1437
Size: 3656
HaeII r_GCGC'y
Cuts at: 1090 1338 2360 3339 1090
Size: 248 1022 979 1407
Fragments arranged by size:
1407 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1498 1528 1530 1560 1498
Size: 30 2 30 3594
Fragments arranged by size:
3594 30 30 2
HincII GTy'rAC
Cuts at: 695 695
Size: 3656
KasI G'GCGC_C
Cuts at: 1086 1334 1086
Size: 248 3408
KpnI G_GTAC'C
Cuts at: 676 676
Size: 3656
MluI A'CGCG_T
Cuts at: 3089 3089
Size: 3656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2327 2511 2327
Size: 184 3472
MscI TGG'CCA
Cuts at: 1417 1417
Size: 3656
MslI CAynn'nnrTG
Cuts at: 523 1645 1772 523
Size: 1122 127 2407
Fragments arranged by size:
2407 1122 127
MspA1I CmG'CkG
Cuts at: 767 1081 1441 2454 2699 767
Size: 314 360 1013 245 1724
Fragments arranged by size:
1724 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1837 1837
Size: 3656
NarI GG'CG_CC
Cuts at: 1087 1335 1087
Size: 248 3408
NgoMIV G'CCGG_C
Cuts at: 1835 1835
Size: 3656
NspI r_CATG'y
Cuts at: 709 1209 2116 709
Size: 500 907 2249
Fragments arranged by size:
2249 907 500
PciI A'CATG_T
Cuts at: 2112 2112
Size: 3656
PmeI GTTT'AAAC
Cuts at: 655 655
Size: 3656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2795 2827 2795
Size: 32 3624
PstI C_TGCA'G
Cuts at: 287 317 703 1388 287
Size: 30 386 685 2555
Fragments arranged by size:
2555 685 386 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3656
PvuII CAG'CTG
Cuts at: 1081 1441 1081
Size: 360 3296
RsrII CG'GwC_CG
Cuts at: 1851 1851
Size: 3656
SacI G_AGCT'C
Cuts at: 670 670
Size: 3656
SalI G'TCGA_C
Cuts at: 693 693
Size: 3656
SapI GCTCTTCn'nnn_
Cuts at: 1679 1889 1679
Size: 210 3446
SbfI CC_TGCA'GG
Cuts at: 703 703
Size: 3656
ScaI AGT'ACT
Cuts at: 3173 3173
Size: 3656
SexAI A'CCwGG_T
Cuts at: 533 533
Size: 3656
SfcI C'TryA_G
Cuts at: 33 283 313 699 1384 2377 2568 33
Size: 250 30 386 685 993 191 1121
Fragments arranged by size:
1121 993 685 386 250 191 30
SfoI GGC'GCC
Cuts at: 1088 1336 1088
Size: 248 3408
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
SmaI CCC'GGG
Cuts at: 678 678
Size: 3656
SmlI C'TyrA_G
Cuts at: 379 811 1726 2218 2480 379
Size: 432 915 492 262 1555
Fragments arranged by size:
1555 915 492 432 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3656
SphI G_CATG'C
Cuts at: 709 1209 709
Size: 500 3156
SspI AAT'ATT
Cuts at: 498 498
Size: 3656
StyI C'CwwG_G
Cuts at: 155 790 1144 155
Size: 635 354 2667
Fragments arranged by size:
2667 635 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1239 1372 1831 2040 1239
Size: 133 459 209 2855
Fragments arranged by size:
2855 459 209 133
TatI w'GTAC_w
Cuts at: 3171 3171
Size: 3656
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 381 413 3173 3205 381
Size: 32 2760 32 832
Fragments arranged by size:
2760 832 32 32
Tth111I GACn'n_nGTC
Cuts at: 214 1453 214
Size: 1239 2417
XbaI T'CTAG_A
Cuts at: 687 687
Size: 3656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1151 1151
Size: 3656
XmaI C'CCGG_G
Cuts at: 676 676
Size: 3656
XmnI GAAnn'nnTTC
Cuts at: 467 467
Size: 3656
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI