Restriction map for
pF1KE0191
(Circular) (Six-base) MAPSORT of: pF1KE0191.seq Check: 6753 from: 1 to: 3506
pF1KE0191 3506 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 544 544
Size: 3506
Acc65I G'GTAC_C
Cuts at: 522 522
Size: 3506
AclI AA'CG_TT
Cuts at: 3346 3346
Size: 3506
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 311 1331 1763 2510 2778 232
Size: 79 1020 432 747 268 960
Fragments arranged by size:
1020 960 747 432 268 79
AflIII A'CryG_T
Cuts at: 858 1962 2939 858
Size: 1104 977 1425
Fragments arranged by size:
1425 1104 977
AgeI A'CCGG_T
Cuts at: 1894 1894
Size: 3506
AlwNI CAG_nnn'CTG
Cuts at: 811 2378 811
Size: 1567 1939
ApaLI G'TGCA_C
Cuts at: 2276 2276
Size: 3506
ApoI r'AATT_y
Cuts at: 429 510 2859 3115 429
Size: 81 2349 256 820
Fragments arranged by size:
2349 820 256 81
AseI AT'TA_AT
Cuts at: 20 20
Size: 3506
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3506
AvaI C'yCGr_G
Cuts at: 526 526
Size: 3506
BamHI G'GATC_C
Cuts at: 531 531
Size: 3506
BanI G'GyrC_C
Cuts at: 111 522 936 1184 1219 111
Size: 411 414 248 35 2398
Fragments arranged by size:
2398 414 411 248 35
BanII G_rGCy'C
Cuts at: 520 1550 520
Size: 1030 2476
BbeI G_GCGC'C
Cuts at: 115 940 1188 115
Size: 825 248 2433
Fragments arranged by size:
2433 825 248
BciVI GTATCCnnnnn_n'
Cuts at: 414 1404 2165 3506 414
Size: 990 761 1341 414
Fragments arranged by size:
1341 990 761 414
BclI T'GATC_A
Cuts at: 1026 1026
Size: 3506
BfrBI ATG'CAT
Cuts at: 448 448
Size: 3506
BglII A'GATC_T
Cuts at: 1021 3058 1021
Size: 2037 1469
BlpI GC'TnA_GC
Cuts at: 618 618
Size: 3506
Bme1580I G_kGCm'C
Cuts at: 1131 1224 2280 1131
Size: 93 1056 2357
Fragments arranged by size:
2357 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 718 799 1134 3278 718
Size: 81 335 2144 946
Fragments arranged by size:
2144 946 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1873 1873
Size: 3506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1561 2053 2351 682
Size: 879 492 298 1837
Fragments arranged by size:
1837 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 151 3210 151
Size: 3059 447
BsaAI yAC'GTr
Cuts at: 859 859
Size: 3506
BsaBI GATnn'nnATC
Cuts at: 6 1020 3057 6
Size: 1014 2037 455
Fragments arranged by size:
2037 1014 455
BsaHI Gr'CG_yC
Cuts at: 112 937 1185 1887 3383 112
Size: 825 248 702 1496 235
Fragments arranged by size:
1496 825 702 248 235
BsaWI w'CCGG_w
Cuts at: 695 1216 1894 2168 2315 2726 695
Size: 521 678 274 147 411 1475
Fragments arranged by size:
1475 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 931 1689 2266 2762 931
Size: 758 577 496 1675
Fragments arranged by size:
1675 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1048 1094 2302 104
Size: 944 46 1208 1308
Fragments arranged by size:
1308 1208 944 46
BsiHKAI G_wGCw'C
Cuts at: 520 1298 1488 2280 520
Size: 778 190 792 1746
Fragments arranged by size:
1746 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 520 1131 1224 1298 1488 1550 2280 520
Size: 611 93 74 190 62 730 1746
Fragments arranged by size:
1746 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2726 2726
Size: 3506
BspHI T'CATG_A
Cuts at: 2682 2682
Size: 3506
BspMI ACCTGCnnnn'nnnn_
Cuts at: 556 770 1072 1453 556
Size: 214 302 381 2609
Fragments arranged by size:
2609 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1798 1852 3336 3501 50
Size: 1748 54 1484 165 55
Fragments arranged by size:
1748 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1418 1418
Size: 3506
BsrFI r'CCGG_y
Cuts at: 1504 1685 1894 1504
Size: 181 209 3116
Fragments arranged by size:
3116 209 181
BssSI C'ACGA_G
Cuts at: 1777 2135 1777
Size: 358 3148
BstAPI GCAn_nnn'nTGC
Cuts at: 883 883
Size: 3506
BstBI TT'CG_AA
Cuts at: 1867 1867
Size: 3506
BstYI r'GATC_y
Cuts at: 1 531 998 1021 1356 1602 2614 2700 2712
Size: 530 467 23 335 246 1012 86 12
Cuts at: 2712 3058 1
Size: 346 449
Fragments arranged by size:
1012 530 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3506
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1456 1637 1800 1456
Size: 181 163 3162
Fragments arranged by size:
3162 181 163
BtsI GCAGTG_nn'
Cuts at: 226 490 749 226
Size: 264 259 2983
Fragments arranged by size:
2983 264 259
DraI TTT'AAA
Cuts at: 309 505 309
Size: 196 3310
DrdI GACnn_nn'nnGTC
Cuts at: 1212 2070 1212
Size: 858 2648
EaeI y'GGCC_r
Cuts at: 1091 1265 1656 1683 3028 1091
Size: 174 391 27 1345 1569
Fragments arranged by size:
1569 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1091 1091
Size: 3506
EarI CTCTTCn'nnn_
Cuts at: 1529 1739 1529
Size: 210 3296
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1714 2024 2170 3316 1714
Size: 310 146 1146 1904
Fragments arranged by size:
1904 1146 310 146
EcoICRI GAG'CTC
Cuts at: 518 518
Size: 3506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 311 1331 1763 2510 2778 232
Size: 79 1020 432 747 268 960
Fragments arranged by size:
1020 960 747 432 268 79
EcoO109I rG'GnC_Cy
Cuts at: 645 645
Size: 3506
EcoRI G'AATT_C
Cuts at: 510 510
Size: 3506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1525 1525
Size: 3506
FspI TGC'GCA
Cuts at: 182 1287 182
Size: 1105 2401
HaeII r_GCGC'y
Cuts at: 115 940 1188 2210 3189 115
Size: 825 248 1022 979 432
Fragments arranged by size:
1022 979 825 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 436 468 1348 1378 1380 1410 436
Size: 32 880 30 2 30 2532
Fragments arranged by size:
2532 880 32 30 30 2
HincII GTy'rAC
Cuts at: 545 545
Size: 3506
KasI G'GCGC_C
Cuts at: 111 936 1184 111
Size: 825 248 2433
Fragments arranged by size:
2433 825 248
KpnI G_GTAC'C
Cuts at: 526 526
Size: 3506
MluI A'CGCG_T
Cuts at: 2939 2939
Size: 3506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2177 2361 2177
Size: 184 3322
MscI TGG'CCA
Cuts at: 1267 1267
Size: 3506
MslI CAynn'nnrTG
Cuts at: 1495 1622 1495
Size: 127 3379
MspA1I CmG'CkG
Cuts at: 178 617 931 1291 2304 2549 178
Size: 439 314 360 1013 245 1135
Fragments arranged by size:
1135 1013 439 360 314 245
NaeI GCC'GGC
Cuts at: 1687 1687
Size: 3506
NarI GG'CG_CC
Cuts at: 112 937 1185 112
Size: 825 248 2433
Fragments arranged by size:
2433 825 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3506
NgoMIV G'CCGG_C
Cuts at: 1685 1685
Size: 3506
NsiI A_TGCA'T
Cuts at: 450 450
Size: 3506
NspI r_CATG'y
Cuts at: 559 1059 1966 559
Size: 500 907 2099
Fragments arranged by size:
2099 907 500
PciI A'CATG_T
Cuts at: 1962 1962
Size: 3506
PmeI GTTT'AAAC
Cuts at: 505 505
Size: 3506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 3474
PstI C_TGCA'G
Cuts at: 553 1238 553
Size: 685 2821
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3506
PvuII CAG'CTG
Cuts at: 931 1291 931
Size: 360 3146
RsrII CG'GwC_CG
Cuts at: 1701 1701
Size: 3506
SacI G_AGCT'C
Cuts at: 520 520
Size: 3506
SalI G'TCGA_C
Cuts at: 543 543
Size: 3506
SapI GCTCTTCn'nnn_
Cuts at: 1529 1739 1529
Size: 210 3296
SbfI CC_TGCA'GG
Cuts at: 553 553
Size: 3506
ScaI AGT'ACT
Cuts at: 404 3023 404
Size: 2619 887
SfcI C'TryA_G
Cuts at: 33 549 1234 2227 2418 33
Size: 516 685 993 191 1121
Fragments arranged by size:
1121 993 685 516 191
SfoI GGC'GCC
Cuts at: 113 938 1186 113
Size: 825 248 2433
Fragments arranged by size:
2433 825 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3506
SmaI CCC'GGG
Cuts at: 528 528
Size: 3506
SmlI C'TyrA_G
Cuts at: 661 1576 2068 2330 661
Size: 915 492 262 1837
Fragments arranged by size:
1837 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3506
SphI G_CATG'C
Cuts at: 559 1059 559
Size: 500 3006
StyI C'CwwG_G
Cuts at: 107 640 994 107
Size: 533 354 2619
Fragments arranged by size:
2619 533 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 133 383 1089 1222 1681 1890 133
Size: 250 706 133 459 209 1749
Fragments arranged by size:
1749 706 459 250 209 133
TatI w'GTAC_w
Cuts at: 402 3021 402
Size: 2619 887
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 3474
Tth111I GACn'n_nGTC
Cuts at: 1303 1303
Size: 3506
XbaI T'CTAG_A
Cuts at: 537 537
Size: 3506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1001 1001
Size: 3506
XmaI C'CCGG_G
Cuts at: 526 526
Size: 3506
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI